##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116310 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8454647063881 31.0 31.0 34.0 30.0 34.0 2 32.049746367466255 33.0 31.0 34.0 30.0 34.0 3 32.07988994927349 33.0 31.0 34.0 30.0 34.0 4 35.78438655317685 37.0 35.0 37.0 35.0 37.0 5 35.70426446565214 37.0 35.0 37.0 33.0 37.0 6 35.738148052618 37.0 35.0 37.0 33.0 37.0 7 35.638982030779815 37.0 35.0 37.0 33.0 37.0 8 35.65974550769495 37.0 35.0 37.0 33.0 37.0 9 37.21770269108417 39.0 37.0 39.0 33.0 39.0 10-11 37.157664861146934 39.0 37.0 39.0 33.0 39.0 12-13 37.074189665548964 39.0 37.0 39.0 33.0 39.0 14-15 38.15481901814117 40.0 37.5 41.0 33.0 41.0 16-17 38.03651018828991 40.0 37.0 41.0 33.0 41.0 18-19 38.168287335568735 40.0 37.0 41.0 33.0 41.0 20-21 38.19763992777921 40.0 37.0 41.0 33.0 41.0 22-23 38.04980655145731 40.0 37.0 41.0 33.0 41.0 24-25 37.89754965179262 40.0 37.0 41.0 33.0 41.0 26-27 37.62345026223025 40.0 36.0 41.0 32.5 41.0 28-29 37.36875591092769 39.5 36.0 41.0 32.0 41.0 30-31 36.974353022096125 39.0 35.0 41.0 31.5 41.0 32-33 36.503469177198866 39.0 35.0 41.0 30.5 41.0 34-35 36.22097841974035 38.5 35.0 41.0 30.0 41.0 36-37 35.931205399363776 38.0 35.0 41.0 30.0 41.0 38-39 35.5645387326971 38.0 35.0 41.0 28.5 41.0 40-41 35.315974550769496 38.0 35.0 40.5 27.0 41.0 42-43 34.887520419568396 38.0 35.0 40.0 25.5 41.0 44-45 34.38674662539765 38.0 34.0 40.0 23.5 41.0 46-47 33.73343220703293 38.0 33.0 40.0 18.0 41.0 48-49 33.13348379331097 38.0 33.0 40.0 8.5 41.0 50-51 32.715020204625574 37.5 33.0 40.0 2.0 41.0 52-53 32.47547502364371 37.0 33.0 40.0 2.0 41.0 54-55 32.10698994067578 37.0 32.0 40.0 2.0 41.0 56-57 31.668192760725645 36.0 31.0 40.0 2.0 41.0 58-59 31.185947038087868 36.0 31.0 40.0 2.0 41.0 60-61 30.586716533402114 35.0 29.5 39.5 2.0 41.0 62-63 30.056886768119682 35.0 28.5 39.0 2.0 40.5 64-65 29.48605021064397 35.0 28.0 39.0 2.0 40.0 66-67 28.847033789012123 35.0 26.5 38.0 2.0 40.0 68-69 28.419284670277705 35.0 26.0 38.0 2.0 40.0 70-71 27.996513627375116 35.0 25.5 37.0 2.0 39.5 72-73 27.52819619981085 34.0 24.5 36.5 2.0 39.0 74-75 27.061129739489296 34.0 23.0 36.0 2.0 39.0 76-77 25.261555326283208 31.5 20.0 34.5 2.0 36.5 78-79 26.07467113747743 33.0 20.0 35.0 2.0 37.0 80-81 26.076149944114867 34.0 20.0 35.0 2.0 37.0 82-83 25.725977989854698 33.0 17.5 35.0 2.0 36.5 84-85 25.321954260166798 33.0 10.5 35.0 2.0 36.0 86-87 24.75273407273665 33.0 4.0 35.0 2.0 35.5 88-89 24.171262144269626 32.0 2.0 35.0 2.0 35.0 90-91 23.6590705872238 32.0 2.0 35.0 2.0 35.0 92-93 23.348903791591436 32.0 2.0 35.0 2.0 35.0 94-95 23.007325251483106 31.0 2.0 34.0 2.0 35.0 96-97 22.440899320780673 31.0 2.0 34.0 2.0 35.0 98-99 21.69788496259995 31.0 2.0 34.0 2.0 35.0 100-101 18.82539334537013 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12575110011061952 1101 2 0.15047324923029493 1101 3 -0.30750960467070243 1101 4 0.1355815842816881 1101 5 0.07106067623539047 1101 6 0.18916134729854406 1101 7 0.12374935298293366 1101 8 0.15294344452266984 1101 9 0.2627077206881765 1101 10-11 0.22065748059629442 1101 12-13 0.056781517458993847 1101 14-15 0.3788218629157285 1101 16-17 0.3451744133594872 1101 18-19 0.34790746332495814 1101 20-21 0.5938713231247306 1101 22-23 0.31492595221602215 1101 24-25 0.6676604461894158 1101 26-27 0.30123233700916074 1101 28-29 0.4467795577421967 1101 30-31 0.6182130017408767 1101 32-33 -0.07595217316098513 1101 34-35 0.4896244892981514 1101 36-37 0.6157950676900512 1101 38-39 0.6622559874735927 1101 40-41 0.46352328797472353 1101 42-43 0.3944309985690708 1101 44-45 0.35527688776138433 1101 46-47 -0.05661153484343373 1101 48-49 0.18421119282513132 1101 50-51 0.11054043769983224 1101 52-53 -0.3934691527040286 1101 54-55 -0.43577240450634136 1101 56-57 0.06513620296636091 1101 58-59 -0.16098962515278714 1101 60-61 -0.03562486062462966 1101 62-63 -0.793750048425057 1101 64-65 -0.9836212639676312 1101 66-67 -0.7010764025002345 1101 68-69 -0.5280924596988861 1101 70-71 -0.32611799122889096 1101 72-73 -0.36530626585104287 1101 74-75 -0.31128239400144153 1101 76-77 -0.4134836566695519 1101 78-79 -1.0084415534556932 1101 80-81 -0.6614339756377348 1101 82-83 -0.40859829514291945 1101 84-85 -1.5821782386615233 1101 86-87 -1.086249980997973 1101 88-89 -1.064292879007322 1101 90-91 -1.638060188082438 1101 92-93 -1.2413577778119596 1101 94-95 -1.1214475674875253 1101 96-97 -0.18470792541303283 1101 98-99 -0.3497366276610272 1101 100-101 -0.35430442714546473 1102 1 0.5105742264801911 1102 2 0.5325212896854126 1102 3 0.31726915639124087 1102 4 0.015059038980382411 1102 5 0.21728907910095785 1102 6 0.3334512756592716 1102 7 0.4292697912467318 1102 8 0.5177137774345937 1102 9 0.5013086477846755 1102 10-11 0.5160219980847032 1102 12-13 0.4530731314497203 1102 14-15 0.22875865179057087 1102 16-17 0.5241461790569133 1102 18-19 0.7611396588580419 1102 20-21 0.47225311832068684 1102 22-23 0.3887144056504894 1102 24-25 0.5925234887515671 1102 26-27 0.38429175546680483 1102 28-29 0.806113733890534 1102 30-31 0.14965000131947903 1102 32-33 0.6599517459287796 1102 34-35 1.1440281976841575 1102 36-37 0.9160900529407883 1102 38-39 1.4381936191634352 1102 40-41 1.301449963069544 1102 42-43 1.609138120355837 1102 44-45 1.4376199134672376 1102 46-47 1.2537129489323746 1102 48-49 0.8268576319317447 1102 50-51 1.0234355072320653 1102 52-53 0.8521271389099567 1102 54-55 1.0624155432391333 1102 56-57 1.263871980463204 1102 58-59 1.4048763672618811 1102 60-61 1.7409027415666891 1102 62-63 0.76916609148223 1102 64-65 0.5780727941866068 1102 66-67 0.9767575629443499 1102 68-69 0.8858561285859885 1102 70-71 0.8936460205705146 1102 72-73 1.1584189764582682 1102 74-75 1.0926788365084654 1102 76-77 0.7576920702583898 1102 78-79 0.543433709923999 1102 80-81 1.1844151604768847 1102 82-83 1.6026111443851043 1102 84-85 1.6893346142672563 1102 86-87 2.418006234762668 1102 88-89 2.066554150069795 1102 90-91 1.9804817419639171 1102 92-93 2.7894049697649486 1102 94-95 2.2951137473038763 1102 96-97 3.0044197610597827 1102 98-99 3.3581858333587817 1102 100-101 2.2393323195886268 1103 1 -0.13326989710310144 1103 2 0.176287785571148 1103 3 0.30477109708368033 1103 4 -0.00978182423961016 1103 5 0.06855441057123102 1103 6 -8.1359004481385E-4 1103 7 0.20896238556438362 1103 8 -0.08064051537707684 1103 9 -0.09293137705618904 1103 10-11 0.2863216409972935 1103 12-13 0.22896196858680895 1103 14-15 0.2295737426149813 1103 16-17 0.023369902081292082 1103 18-19 -0.562994792314143 1103 20-21 -0.18319634604820578 1103 22-23 -0.07154021319751536 1103 24-25 -0.10965784954993296 1103 26-27 0.24308697360063292 1103 28-29 0.5190853221532237 1103 30-31 0.3012957085078014 1103 32-33 -0.03547598268480101 1103 34-35 -0.06626523501262938 1103 36-37 -1.0219242305555625 1103 38-39 -0.6506512806968416 1103 40-41 -0.17883260174958338 1103 42-43 0.14317786573698754 1103 44-45 -0.172417347827583 1103 46-47 0.019704254630248386 1103 48-49 0.29010091713590214 1103 50-51 0.3571569790532152 1103 52-53 0.6150521505541136 1103 54-55 0.6954306030124542 1103 56-57 0.8503492355478137 1103 58-59 1.121341201914884 1103 60-61 -0.18011107616347388 1103 62-63 0.5938439365373505 1103 64-65 0.5676318688644493 1103 66-67 0.13100379800102147 1103 68-69 -0.4475160185961258 1103 70-71 -0.023486412281524593 1103 72-73 -0.10803809542497689 1103 74-75 -0.16015457445256587 1103 76-77 0.20393488969636664 1103 78-79 0.6069719803788978 1103 80-81 0.46600963338481804 1103 82-83 0.3966648627518161 1103 84-85 0.3593004580803303 1103 86-87 0.06487783855089546 1103 88-89 -0.4320873652228592 1103 90-91 -0.7059799875811841 1103 92-93 -0.7256936174109576 1103 94-95 0.16050731973051668 1103 96-97 -0.9124021610020066 1103 98-99 -0.8843230938264384 1103 100-101 -1.047036256719398 1104 1 -0.13765586201538227 1104 2 -0.22464990347906522 1104 3 -0.40061305294656435 1104 4 -0.04093729913374489 1104 5 -0.22984244363324535 1104 6 -0.41067444908897954 1104 7 -0.09094687033397975 1104 8 -0.24639974102741036 1104 9 0.007945815926269972 1104 10-11 -0.1784393995350726 1104 12-13 -0.3757718487150683 1104 14-15 -0.6265968562960964 1104 16-17 -0.34021146512984046 1104 18-19 -0.34104984373580294 1104 20-21 0.1949796975089697 1104 22-23 -0.2354845568151802 1104 24-25 -0.3139076983097624 1104 26-27 0.16625696755102837 1104 28-29 0.17539203721674568 1104 30-31 -0.16187731024597696 1104 32-33 -0.2739817298112328 1104 34-35 -0.708733474577059 1104 36-37 -0.6770210242639649 1104 38-39 -0.6082284131832196 1104 40-41 0.04061688403383812 1104 42-43 0.4594966800140625 1104 44-45 0.5054410913738607 1104 46-47 -0.11724371875148165 1104 48-49 0.5503852486543579 1104 50-51 0.09785570863579096 1104 52-53 -0.02355946469089787 1104 54-55 -0.22645384949813518 1104 56-57 0.15245147390232106 1104 58-59 0.4439364832215986 1104 60-61 1.297051658258784 1104 62-63 1.2781300829378317 1104 64-65 1.05160041090922 1104 66-67 0.5392766352665994 1104 68-69 1.040914108445616 1104 70-71 0.7672728133688089 1104 72-73 1.0624851955611057 1104 74-75 1.3601872283302505 1104 76-77 1.0302355551531086 1104 78-79 0.3600724038513725 1104 80-81 1.0534264512916565 1104 82-83 0.999735038190412 1104 84-85 1.0309580503368316 1104 86-87 1.1484380321383156 1104 88-89 1.682628303258685 1104 90-91 1.0557411255464615 1104 92-93 0.865579824815299 1104 94-95 0.8896361763548377 1104 96-97 0.2022378873948476 1104 98-99 -0.11972841748040253 1104 100-101 1.0415904825425493 1105 1 0.1716661718829222 1105 2 0.03207131218429282 1105 3 0.2823209038038712 1105 4 0.2594718183414173 1105 5 -0.18761567566129855 1105 6 0.32895150064801726 1105 7 0.11931028922183629 1105 8 0.3222735494459954 1105 9 0.7409966633838962 1105 10-11 0.15645247656077998 1105 12-13 0.40863785321363366 1105 14-15 -0.08933794337966816 1105 16-17 -0.04740024362195072 1105 18-19 -0.08001828324485416 1105 20-21 -0.41424005702054956 1105 22-23 0.22477260274063582 1105 24-25 0.0396136342442901 1105 26-27 0.011522893909884147 1105 28-29 0.40267140600693097 1105 30-31 0.6237012987546038 1105 32-33 0.5167839043208531 1105 34-35 0.8061700905895108 1105 36-37 0.9146651241872235 1105 38-39 1.1997055435672124 1105 40-41 0.845847743180542 1105 42-43 1.0744148565190272 1105 44-45 1.0459144987379787 1105 46-47 0.8902811205237953 1105 48-49 1.0687955350365854 1105 50-51 1.512598549079975 1105 52-53 0.8679513476995169 1105 54-55 0.5608781201102815 1105 56-57 0.47821126349904475 1105 58-59 0.6750878566874086 1105 60-61 -0.06823180170614407 1105 62-63 0.1934573769179586 1105 64-65 -0.44270116711532737 1105 66-67 -0.6891716405954718 1105 68-69 -0.8558567857682959 1105 70-71 -0.8077564093079914 1105 72-73 -1.1159923029777623 1105 74-75 -1.3998215384727892 1105 76-77 -1.0358807511005423 1105 78-79 -0.8911695599125089 1105 80-81 -1.0188996737814016 1105 82-83 -0.5786547923180656 1105 84-85 -0.9387964791780696 1105 86-87 -0.20303771303994367 1105 88-89 -0.6006124915981239 1105 90-91 -0.20835881600818595 1105 92-93 -0.37251193438984487 1105 94-95 -0.48222238588946453 1105 96-97 -0.4963302014420883 1105 98-99 -0.19037423863762015 1105 100-101 0.13145021369392396 1106 1 0.29495283363678837 1106 2 0.1392931250066951 1106 3 -0.18577047423591964 1106 4 0.1310267188572638 1106 5 -0.01672400285363551 1106 6 0.49682180278508525 1106 7 0.17509510867651557 1106 8 0.40967222299057937 1106 9 0.6101197289697495 1106 10-11 0.23887694229402 1106 12-13 0.017444043338912252 1106 14-15 0.09559204925113107 1106 16-17 0.5182592994878448 1106 18-19 0.7445948339253761 1106 20-21 0.3901446150501968 1106 22-23 0.467513943934442 1106 24-25 0.5003726615103261 1106 26-27 0.03042488359300677 1106 28-29 -0.23686433459734246 1106 30-31 0.1633475773102404 1106 32-33 0.21162546659056858 1106 34-35 0.21840295720704717 1106 36-37 0.39550521261759286 1106 38-39 0.4837880785709032 1106 40-41 0.7057593128194526 1106 42-43 0.028799528589772194 1106 44-45 0.39771995194357856 1106 46-47 0.8375913637072898 1106 48-49 0.6278964930321678 1106 50-51 -0.4540558355920936 1106 52-53 -1.4657258814824985 1106 54-55 -1.469519816514623 1106 56-57 -2.208155722499477 1106 58-59 -2.2137846562086843 1106 60-61 -2.702498566628769 1106 62-63 -2.8132117461476263 1106 64-65 -3.246975301234709 1106 66-67 -2.8956933797259055 1106 68-69 -3.0446556066968107 1106 70-71 -2.9637991506491836 1106 72-73 -2.7829046095363488 1106 74-75 -2.512938708701231 1106 76-77 -2.209756948595018 1106 78-79 -2.439290414739336 1106 80-81 -2.1616410149752134 1106 82-83 -1.592656266157416 1106 84-85 -2.1258635388685647 1106 86-87 -1.6168918029441421 1106 88-89 -1.3742307672060825 1106 90-91 -1.4239815131341977 1106 92-93 -0.9709644030707558 1106 94-95 -0.7717581264937401 1106 96-97 -1.18719239746272 1106 98-99 -0.5617449092345232 1106 100-101 0.19331462047358627 1107 1 0.06886587711505143 1107 2 -0.12157644021069913 1107 3 -0.620553082931572 1107 4 -0.2689732811427419 1107 5 -0.37324574198407134 1107 6 -0.1466564580844789 1107 7 -0.16403532610609517 1107 8 -0.25989299440072955 1107 9 -0.19857592320416728 1107 10-11 -0.526340449010334 1107 12-13 -0.095599434921958 1107 14-15 -0.5739731681401707 1107 16-17 -0.49478417877301695 1107 18-19 -0.26844864433549276 1107 20-21 -0.3968119066889386 1107 22-23 -0.48465996910903897 1107 24-25 -0.595279512402719 1107 26-27 -0.7913142468417789 1107 28-29 -1.102081725901698 1107 30-31 -1.4888263357332434 1107 32-33 -1.1666354029746486 1107 34-35 -0.8120318254016468 1107 36-37 -0.7392773960780659 1107 38-39 -0.816211921429101 1107 40-41 -1.785545035006642 1107 42-43 -2.053809167062397 1107 44-45 -1.315323526317293 1107 46-47 -1.301539071075318 1107 48-49 -2.1894948113156616 1107 50-51 -2.041012357331226 1107 52-53 -1.7613780553955465 1107 54-55 -1.3956067730363628 1107 56-57 -1.5603296355429563 1107 58-59 -1.926828134469556 1107 60-61 -1.7894550883678981 1107 62-63 -1.3610378331041453 1107 64-65 -1.6295839968868826 1107 66-67 -2.117432510160686 1107 68-69 -1.6924816936533347 1107 70-71 -1.7855382810839657 1107 72-73 -1.4481220008406943 1107 74-75 -1.0129387087012311 1107 76-77 -1.0401917312037128 1107 78-79 -1.4653773712610736 1107 80-81 -0.8399018845404278 1107 82-83 -0.48830844007045826 1107 84-85 -0.5780374519120421 1107 86-87 -0.3125439768571887 1107 88-89 -0.6698829411191234 1107 90-91 -1.0674597740037655 1107 92-93 -0.7057470117664089 1107 94-95 -0.33262769171112794 1107 96-97 -0.4089315278975043 1107 98-99 -1.183484039669306 1107 100-101 -1.1979897273525033 1108 1 0.3272974090126546 1108 2 0.18358406364951918 1108 3 0.4532380588807001 1108 4 0.13647165993812393 1108 5 0.42041537138447893 1108 6 0.29267714614144325 1108 7 0.37030030981426165 1108 8 0.17448327540829212 1108 9 0.3934598346178575 1108 10-11 0.32083549572757875 1108 12-13 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>>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 17.0 6 57.0 7 74.0 8 163.0 9 257.0 10 438.0 11 632.0 12 904.0 13 1279.0 14 1342.0 15 1688.0 16 2064.0 17 2654.0 18 2576.0 19 1685.0 20 1621.0 21 1709.0 22 1815.0 23 1816.0 24 2040.0 25 2114.0 26 1889.0 27 2008.0 28 2309.0 29 2699.0 30 3114.0 31 3606.0 32 4380.0 33 5339.0 34 6920.0 35 10103.0 36 12260.0 37 18104.0 38 15314.0 39 1315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.92364636027542 23.299063245977646 16.252294487198267 20.52499590654866 2 15.24116584988393 23.911959418794602 40.19860717049265 20.64826756082882 3 16.85409681024847 30.45395924684034 34.150116069125616 18.541827873785575 4 11.585418278737855 22.434872323961827 36.334794944544754 29.644914452755565 5 10.608015135878913 40.356037151702786 34.67836257309941 14.357585139318886 6 24.198235688614517 40.27221295547951 18.608670231974276 16.920881123931697 7 21.788324305734676 38.3956667526438 21.606912561258707 18.209096380362823 8 23.984180208064654 35.39420514143238 21.607772332559538 19.01384231794343 9 24.588599432550943 17.92107299458344 22.07892700541656 35.41140056744906 10-11 21.761241509758406 29.445877396612502 27.997592640357666 20.79528845327143 12-13 24.129911443556015 28.1299974206861 28.157080216662365 19.58301091909552 14-15 21.989080904479408 28.604591178746453 27.693233599862438 21.7130943169117 16-17 18.685031855348345 32.43699863292837 28.583834163034037 20.294135348689245 18-19 18.03800189149686 28.89605364972917 33.52205313386639 19.543891324907577 20-21 18.8436076003783 27.86733728828132 33.55687387155017 19.73218123979022 22-23 18.942481299974208 29.54432121055799 33.05132834665979 18.461869142808013 24-25 18.803198349239103 30.121657639068005 33.6033015217952 17.47184248989769 26-27 18.084232880657904 29.512030505079164 34.55336454343406 17.850372070828875 28-29 17.130083397816183 28.25724357320953 35.84730461697189 18.765368412002406 30-31 17.78995787120626 28.648439515088985 34.13507007136102 19.426532542343736 32-33 18.436076003782993 29.220617315794 34.85383887885823 17.489467801564786 34-35 18.220393633760672 29.97738626495043 34.23616305964695 17.566057041641947 36-37 17.933261386453637 29.444618817692053 33.375326233236876 19.246793562617434 38-39 17.626219415196633 28.802920147383176 34.262288738601235 19.30857169881896 40-41 17.32395516709158 28.63579495866241 35.169413191055774 18.87083668319024 42-43 18.27006994871001 28.57277976259571 34.66136999729147 18.4957802914028 44-45 17.249547523956508 29.352260660593526 34.27511403256108 19.12307778288888 46-47 18.71910170322159 28.636219037219817 32.30059582663423 20.344083432924364 48-49 18.403528607601466 28.61018085833552 32.36878420380632 20.617506330256692 50-51 17.731923308399967 29.0916516206689 32.58662195855903 20.58980311237211 52-53 17.480870088556443 29.679305304788926 33.53108073252515 19.30874387412948 54-55 17.427994153555154 29.58129137649385 31.976614220617318 21.01410024933368 56-57 18.443893233140887 30.35736547745455 30.43388545217717 20.764855837227397 58-59 17.134885800386037 30.37370120239533 32.25187968308694 20.23953331413169 60-61 18.280928920873208 31.886300910521292 29.98615732501053 19.84661284359497 62-63 17.19650756393557 32.58919167558691 29.952239088286753 20.262061672190768 64-65 17.235553568511467 32.15400355948379 30.19241847148544 20.418024400519304 66-67 17.212723606770215 31.925092538057754 29.83745115151307 21.02473270365896 68-69 17.172642077207463 31.368755910927693 29.965609147966642 21.492992863898202 70-71 17.510962084085634 30.56229043074542 30.020204625569598 21.906542859599345 72-73 17.671739317341586 29.851689450606138 29.777749118734416 22.698822113317856 74-75 17.43014358180724 29.93035852463245 29.460493508726675 23.179004384833636 76-77 17.24679324495221 29.488381696332556 30.365453935426146 22.899371123289086 78-79 17.2476432353321 29.184710085661404 30.236752615040245 23.33089406396625 80-81 17.113538563389604 29.204228409789483 29.8804472587988 23.801785768022114 82-83 17.183892975208387 28.828685532996012 29.64676144253049 24.340660049265107 84-85 17.184248989768722 28.32516550597541 29.583440804745937 24.90714469950993 86-87 17.135921912254577 28.194479113766064 29.70983929693485 24.95975967704451 88-89 17.59965487489215 27.606988783433994 29.73295944779983 25.06039689387403 90-91 17.211526427316787 27.166599772993084 29.95757646378463 25.664297335905502 92-93 17.12449488436076 27.339437709569253 29.79881351560485 25.737253890465134 94-95 16.919869314762273 26.62754707247872 30.028802338577936 26.423781274181067 96-97 17.41853666924598 26.073854354741638 29.622560398933885 26.885048577078496 98-99 17.211761671395408 26.183905081248387 29.637176511048064 26.96715673630814 100-101 17.329120453959245 25.778952798555583 30.324133780414407 26.56779296707076 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1350.0 1 834.0 2 352.5 3 383.5 4 319.0 5 291.0 6 439.0 7 703.5 8 924.5 9 961.0 10 744.5 11 514.0 12 452.5 13 425.5 14 431.5 15 408.5 16 381.0 17 398.0 18 419.5 19 436.0 20 464.5 21 498.5 22 542.5 23 620.5 24 684.5 25 832.0 26 1080.5 27 1309.0 28 1391.5 29 1506.0 30 1772.0 31 2122.0 32 2535.0 33 2950.5 34 3419.0 35 3699.5 36 3996.5 37 4369.0 38 4260.5 39 3926.5 40 3747.0 41 3706.0 42 3646.5 43 3638.5 44 3738.5 45 3849.5 46 3805.0 47 3576.5 48 3413.0 49 3232.0 50 3068.0 51 2921.0 52 2627.0 53 2259.5 54 1892.5 55 1596.5 56 1480.5 57 1412.0 58 1308.5 59 1205.0 60 1089.0 61 950.5 62 816.0 63 661.0 64 529.0 65 461.5 66 388.0 67 373.0 68 351.5 69 298.5 70 272.0 71 254.0 72 227.5 73 202.5 74 179.0 75 144.5 76 118.5 77 108.0 78 89.0 79 58.5 80 42.5 81 30.0 82 23.0 83 17.5 84 12.0 85 6.5 86 3.0 87 2.0 88 1.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23299802252600807 2 0.0 3 0.0 4 0.0 5 0.025793139025019347 6 0.0034390852033359125 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0025793139025019345 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0012896569512509672 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007737941707505804 36-37 0.017625311667096553 38-39 0.012466683862092683 40-41 0.009887369959590749 42-43 0.009027598658756771 44-45 0.00472874215458688 46-47 8.597713008339781E-4 48-49 0.0030091995529189235 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.2988565041698906E-4 58-59 4.2988565041698906E-4 60-61 0.0025793139025019345 62-63 0.0012896569512509672 64-65 8.597713008339781E-4 66-67 0.005588513455420858 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.061043762359212446 78-79 0.13326455162926662 80-81 0.0012896569512509672 82-83 4.2988565041698906E-4 84-85 0.0 86-87 0.1143495830109191 88-89 0.35250623334193104 90-91 0.3907660562290431 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 116310.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.093715071790896 #Duplication Level Percentage of deduplicated Percentage of total 1 86.0175978253094 51.69117014874044 2 7.501251877816725 9.015561860545095 3 2.1889977823878675 3.94635027082796 4 1.0730381286215036 2.5793139025019345 5 0.692467272337077 2.080646548018227 6 0.4535374490306889 1.6352850141862263 7 0.3448029186637099 1.450434184506921 8 0.2718363259174476 1.3068523772676468 9 0.22891480077258747 1.2380706732009286 >10 1.1431432863581086 12.161465050296622 >50 0.0557979826883182 2.158025965093285 >100 0.017168610057944057 2.1133178574499185 >500 0.005722870019314687 2.8931304273063363 >1k 0.005722870019314687 5.730375720058464 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 2021 1.7375977989854698 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1690 1.4530134984094232 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1566 1.34640185710601 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1388 1.1933625655575617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1000 0.8597713008339782 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 936 0.8047459375806035 RNA PCR Primer, Index 26 (100% over 22bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.7737941707505804 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 529 0.4548190181411745 RNA PCR Primer, Index 26 (100% over 24bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 421 0.3619637176511048 RNA PCR Primer, Index 26 (100% over 29bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.275986587567707 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 272 0.23385779382684205 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 248 0.21322328260682658 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.17969220187430143 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 197 0.1693749462642937 RNA PCR Primer, Index 26 (100% over 27bp) TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 180 0.15475883415011607 RNA PCR Primer, Index 26 (95% over 22bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.12552660992176082 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 128 0.1100507265067492 RNA PCR Primer, Index 26 (96% over 27bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.10059324219757544 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0012896569512509672 0.0 22-23 0.0 0.0 0.0 0.005158627805003869 0.0 24-25 0.0 0.0 0.0 0.01461611211417763 0.0 26-27 0.0 0.0 0.0 0.03654028028544407 0.0 28-29 0.0 0.0 0.0 0.09156564353881867 0.0 30-31 0.0 0.0 0.0 0.20634511220015478 0.0 32-33 0.0 0.0 0.0 0.3740005158627805 0.0 34-35 0.0 0.0 0.0 0.6413893904221477 0.0 36-37 0.0 0.0 0.0 1.0149600206345113 0.0 38-39 0.0 0.0 0.0 1.4762273235319405 0.0 40-41 0.0 0.0 0.0 1.9628578798039722 0.0 42-43 0.0 0.0 0.0 2.3837159315622047 0.0 44-45 0.0 0.0 0.0 2.8471326627117186 0.0 46-47 0.0 0.0 0.0 3.3565471584558506 0.0 48-49 0.0 0.0 0.0 3.857363941191643 0.0 50-51 0.0 0.0 0.0 4.409337116327057 0.0 52-53 0.0 0.0 0.0 4.8658756770699 0.0 54-55 0.0 0.0 0.0 5.408821253546557 0.0 56-57 0.0 0.0 0.0 6.064396870432464 0.0 58-59 0.0 0.0 0.0 6.72040237296879 0.0 60-61 0.0 0.0 0.0 7.402201014530135 0.0 62-63 0.0 0.0 0.0 8.055627203163958 0.0 64-65 0.0 0.0 0.0 8.703464878342361 0.0 66-67 0.0 0.0 0.0 9.430831398847907 0.0 68-69 0.0 0.0 0.0 10.246754363339353 0.0 70-71 0.0 0.0 0.0 11.192502794256729 0.0 72-73 0.0 0.0 0.0 12.056143065944458 0.0 74-75 0.0 0.0 0.0 12.939987963201787 0.0 76-77 0.0 0.0 0.0 13.853065084687472 0.0 78-79 0.0 0.0 0.0 14.83148482503654 0.0 80-81 0.0 0.0 0.0 15.79098959676726 0.0 82-83 0.0 0.0 0.0 16.791333505287596 0.0 84-85 0.0 0.0 0.0 17.795546384661677 0.0 86-87 0.0 0.0 0.0 18.79202132232826 0.0 88-89 0.0 0.0 0.0 19.846530822801135 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTCAG 15 6.625822E-4 94.91702 8 TGAGATG 50 0.0016167747 37.966812 4 GGTATCA 1070 0.0 34.65552 1 TATGAGA 70 2.052553E-4 33.898937 2 CATAGAC 60 0.0039533675 31.639006 2 CCAGTTG 30 0.0039614406 31.639006 12-13 GACTTTA 65 0.005847697 29.205238 7 TTATAGG 65 0.005847697 29.205238 3 AGACGAG 65 0.005847697 29.205238 5 GTATCAA 1585 0.0 29.094046 1 TAGGACT 85 6.4049894E-4 27.91677 4 ACATAGA 70 0.008326587 27.165869 1 GACGAGT 70 0.008396847 27.11915 6 GGCTAGT 35 0.008413841 27.11915 12-13 ATGGGAC 90 8.9446E-4 26.365839 7 ATCAACG 1865 0.0 24.683516 3 AACGCAG 1865 0.0 24.683516 6 GCTTATA 135 1.32055375E-5 24.650509 1 TCAACGC 1870 0.0 24.617516 4 CAACGCA 1875 0.0 24.551872 5 >>END_MODULE