FastQCFastQC Report
Thu 26 May 2016
SRR938849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72547
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8111.1178959846720058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8101.1165175679214854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.62580120473624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.4659048616758791No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.35149627138268985No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC1870.2577639323473059No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.23984451459054132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.19573517857389No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.14886900905619804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.10613808979006714No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.10200283953850607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAG405.40847E-447.4035953
GGTATCA5300.041.1711961
ACGCAGC500.001632991437.870538
AACAAGT300.004006034731.55877718-19
GTATCAA7600.031.2081411
TTGAAGT650.005866372529.1714444
GCTTGAT700.00839527927.10651
ATCAACG9850.024.5439913
TATCAAC9950.023.8209022
TCAACGC10200.023.7017974
AACGCAG10450.023.5558326
CAACGCA10350.023.3582945
GAGTACT7600.021.8004712-13
ACGCAGA12000.020.5132057
AGTACTT7500.020.51320512-13
ATCATTA704.7772084E-420.38626956-57
CGCAGAG12100.020.3436748
GTACTTT8200.020.20531314-15
GCAGAGT12200.019.7889049
AGAGTAC11050.018.63538610-11
Produced by FastQC (version 0.11.5)