FastQCFastQC Report
Thu 26 May 2016
SRR938849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72547
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT21252.9291355948557487No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT12471.7188856878988794RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7921.091706066412119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7381.01727156188402No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT6140.8463478848194964RNA PCR Primer, Index 17 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.6712889575034116No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG4640.6395853722414435RNA PCR Primer, Index 17 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.49623003018732686No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT2530.34873943788164913RNA PCR Primer, Index 17 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC2040.28119701710615186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.2632775993493873No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.23984451459054132No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG1710.23570926433898026TruSeq Adapter, Index 12 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.19987042882545109No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT1380.1902215115718086No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC930.12819275779839276No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA880.12130067404579101No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC860.11854384054475031TruSeq Adapter, Index 25 (95% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.11027334004162818No Hit
CTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC770.10613808979006714No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA760.10475967303954677RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAAC250.00502109956.946214
CTAACCT352.783462E-454.2344864
CACTGAC600.00394498631.6367847
TCCCGAC600.00394498631.6367847
CATGGGC1102.7070182E-630.1987464
ATGGGCT650.005835361329.2031845
GGTATCA5950.028.8316781
GTACATG4650.027.6691071
CTCCCGA700.00837922227.1172436
GTATCAA8050.026.6379621
TACATGG4700.026.2517992
GTTTCTG601.7117156E-423.72758916-17
ACATGGG4800.023.7275893
AGTACTT5750.022.28329812-13
CATGGGG1301.2148115E-421.9023884
TATCAAC9800.021.7906422
TCAACGC9850.021.6800294
AACGCAG10000.021.3548286
ATCAACG10050.021.2485853
CAACGCA10200.020.9361065
Produced by FastQC (version 0.11.5)