##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938849_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72547 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.749486539760433 31.0 31.0 34.0 30.0 34.0 2 31.982135718913256 33.0 31.0 34.0 30.0 34.0 3 32.02471501233683 33.0 31.0 34.0 30.0 34.0 4 35.7292789501978 37.0 35.0 37.0 33.0 37.0 5 35.631025404220715 37.0 35.0 37.0 33.0 37.0 6 35.67434904268956 37.0 35.0 37.0 33.0 37.0 7 35.59795718637573 37.0 35.0 37.0 33.0 37.0 8 35.558562035645856 37.0 35.0 37.0 33.0 37.0 9 37.10981846251396 39.0 37.0 39.0 33.0 39.0 10-11 37.09627551794009 39.0 37.0 39.0 33.0 39.0 12-13 36.99880077742705 39.0 37.0 39.0 33.0 39.0 14-15 38.05950625111997 40.0 37.0 41.0 33.0 41.0 16-17 37.9794546983335 40.0 37.0 41.0 32.5 41.0 18-19 38.046059795718634 40.0 37.0 41.0 33.0 41.0 20-21 38.131004727969454 40.0 37.0 41.0 33.0 41.0 22-23 38.08664038485396 40.0 37.0 41.0 33.0 41.0 24-25 37.92214702193061 40.0 37.0 41.0 33.0 41.0 26-27 37.6123271809999 40.0 36.5 41.0 32.0 41.0 28-29 37.310391883882176 39.5 36.0 41.0 31.5 41.0 30-31 36.846389237322015 39.0 35.0 41.0 31.0 41.0 32-33 36.38435083463135 38.5 35.0 41.0 30.0 41.0 34-35 36.035204763808295 38.0 35.0 41.0 30.0 41.0 36-37 35.72637049085421 38.0 35.0 41.0 29.0 41.0 38-39 35.3084827766827 38.0 35.0 41.0 26.5 41.0 40-41 34.947461645553915 38.0 34.5 40.0 25.0 41.0 42-43 34.55980261072133 38.0 34.0 40.0 23.5 41.0 44-45 34.03049057852151 38.0 33.5 40.0 20.5 41.0 46-47 33.28813734544502 38.0 33.0 40.0 9.5 41.0 48-49 32.63301032434146 38.0 33.0 40.0 2.0 41.0 50-51 32.05448192206432 37.5 31.5 40.0 2.0 41.0 52-53 31.734661667608584 37.0 31.0 40.0 2.0 41.0 54-55 31.33864942726784 37.0 31.0 40.0 2.0 41.0 56-57 30.781782844225123 36.0 30.0 40.0 2.0 41.0 58-59 30.08606834190249 35.5 28.5 40.0 2.0 41.0 60-61 29.348146718678926 35.0 27.0 39.0 2.0 41.0 62-63 28.6282065419659 35.0 25.5 39.0 2.0 40.5 64-65 27.905171819647954 35.0 22.5 38.5 2.0 40.0 66-67 27.081629839965814 34.5 15.0 38.0 2.0 40.0 68-69 26.414924118157884 34.0 4.5 37.0 2.0 40.0 70-71 25.92959047238342 34.0 2.0 37.0 2.0 39.0 72-73 25.584593435979436 34.0 2.0 36.0 2.0 39.0 74-75 25.197878616620947 33.5 2.0 36.0 2.0 39.0 76-77 23.467400443850195 30.5 2.0 34.0 2.0 36.0 78-79 24.25118199236357 32.5 2.0 35.0 2.0 37.0 80-81 24.22755592925965 33.0 2.0 35.0 2.0 37.0 82-83 23.916936606613643 33.0 2.0 35.0 2.0 36.0 84-85 23.538795539443395 32.5 2.0 35.0 2.0 36.0 86-87 23.059223675686106 32.0 2.0 35.0 2.0 35.0 88-89 22.58657146401643 31.0 2.0 35.0 2.0 35.0 90-91 22.22117385970474 31.0 2.0 35.0 2.0 35.0 92-93 21.95437440555778 31.0 2.0 34.5 2.0 35.0 94-95 21.606282823548874 31.0 2.0 34.0 2.0 35.0 96-97 21.138792782609894 30.5 2.0 34.0 2.0 35.0 98-99 20.52931892428357 30.0 2.0 34.0 2.0 35.0 100-101 18.35994596606338 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3522980069901358 1101 2 -0.13187161332072606 1101 3 -0.23401741761313843 1101 4 0.3628379489452769 1101 5 -0.142385491171936 1101 6 0.24947706043118245 1101 7 0.28972948388755526 1101 8 0.3457130810887463 1101 9 0.356779950410818 1101 10-11 0.6288085169240318 1101 12-13 0.5385347306562025 1101 14-15 0.78821207969483 1101 16-17 0.5221077719284821 1101 18-19 0.2609487205574865 1101 20-21 -0.03897920522331333 1101 22-23 0.3710847680113858 1101 24-25 1.12737579634166 1101 26-27 0.533277310910222 1101 28-29 0.23933875437376884 1101 30-31 0.02674225275276143 1101 32-33 0.25397644050773494 1101 34-35 -0.32498213464600667 1101 36-37 1.0687207167808523 1101 38-39 0.08548478406932247 1101 40-41 0.4542483012344931 1101 42-43 0.87582201850487 1101 44-45 0.7999412753645601 1101 46-47 0.9329137790196924 1101 48-49 1.4354745374766367 1101 50-51 1.7418813975834695 1101 52-53 1.3312932753948843 1101 54-55 1.3686614817208813 1101 56-57 0.5185770431997447 1101 58-59 1.4550927237177866 1101 60-61 1.6265780473544673 1101 62-63 0.512716712284206 1101 64-65 0.42829918073070417 1101 66-67 0.5388093241997574 1101 68-69 0.8049578807971187 1101 70-71 1.2777928560027192 1101 72-73 0.5746544991060567 1101 74-75 0.6791189604491414 1101 76-77 -0.22146934583741995 1101 78-79 -0.6398926411012695 1101 80-81 -0.3681098469162265 1101 82-83 -0.5632552495922312 1101 84-85 0.10872479491973408 1101 86-87 -0.33060518554394136 1101 88-89 -0.3876485232772069 1101 90-91 0.7904993205051234 1101 92-93 0.686910378990504 1101 94-95 0.82617138073077 1101 96-97 -1.096322543063696 1101 98-99 -0.9348659273086355 1101 100-101 0.042001943139982956 1102 1 0.18079022830398017 1102 2 0.2854077984439769 1102 3 0.21543111179862962 1102 4 0.2663305960041029 1102 5 0.529489508828064 1102 6 0.26602117807824044 1102 7 0.2327441897699103 1102 8 0.6278821987358043 1102 9 0.08839759746964404 1102 10-11 0.3967313110416768 1102 12-13 -0.01293585757908744 1102 14-15 0.64299149145954 1102 16-17 0.45685041898730816 1102 18-19 0.5762060734986605 1102 20-21 0.40081858889433164 1102 22-23 0.20748182683491478 1102 24-25 0.2744346198710659 1102 26-27 0.527303046204338 1102 28-29 -0.07729727503799921 1102 30-31 0.0037643115762904245 1102 32-33 -0.11734708890403311 1102 34-35 -0.03178360523423862 1102 36-37 -1.0199741361603216 1102 38-39 -1.0776585982836195 1102 40-41 -0.6854575811184489 1102 42-43 -1.152211069730427 1102 44-45 -1.604470489341324 1102 46-47 -2.1066082798038366 1102 48-49 -2.1559776684057184 1102 50-51 -1.3114274259459435 1102 52-53 -1.3768868716639382 1102 54-55 -1.7140591065144157 1102 56-57 -1.4837207509179002 1102 58-59 -1.0177933056939779 1102 60-61 -0.8922638644102392 1102 62-63 -0.838386228892265 1102 64-65 -1.2003772898575313 1102 66-67 -1.8196465581531847 1102 68-69 -1.0636082956734683 1102 70-71 -1.1027218498796358 1102 72-73 -1.0963013832468853 1102 74-75 -0.9601273630802716 1102 76-77 -0.649318610543304 1102 78-79 -1.7409955822777405 1102 80-81 -1.6135142586809295 1102 82-83 -1.8909206907687022 1102 84-85 -1.100834028609679 1102 86-87 -0.4119470973086443 1102 88-89 -0.23369631739485186 1102 90-91 -0.20398597361252158 1102 92-93 -0.052519768068318484 1102 94-95 0.745748586613125 1102 96-97 0.496048780465717 1102 98-99 -0.4036159273086355 1102 100-101 0.869207825492925 1103 1 0.4255866083944788 1103 2 0.1618783866792768 1103 3 0.2604537362330177 1103 4 0.39144371817604906 1103 5 0.46410489344344796 1103 6 0.1576501373542598 1103 7 0.15174871465678308 1103 8 -0.03818114968048292 1103 9 -0.005720049589179155 1103 10-11 0.13926524769326676 1103 12-13 -0.14800373088225882 1103 14-15 -0.04976868953593794 1103 16-17 -0.025728766533056557 1103 18-19 -0.3835224332886682 1103 20-21 -0.21049362830024165 1103 22-23 -0.09093446275784345 1103 24-25 -0.11300881904295323 1103 26-27 0.268253272448689 1103 28-29 0.368064715912233 1103 30-31 -0.1888827472472414 1103 32-33 -0.25083125179995847 1103 34-35 0.6536236345847612 1103 36-37 0.40706206293469904 1103 38-39 0.8832011302231706 1103 40-41 0.698479070465261 1103 42-43 0.9492595185048671 1103 44-45 1.63647973690302 1103 46-47 0.9552695482504632 1103 48-49 0.9461716528612527 1103 50-51 0.5567852437373162 1103 52-53 0.01146154462565363 1103 54-55 0.008565327874727302 1103 56-57 -0.42048545680025384 1103 58-59 -1.0339697762822162 1103 60-61 -0.6533498372609152 1103 62-63 -0.23103328771579257 1103 64-65 -0.4726623577308331 1103 66-67 -1.1664791373387011 1103 68-69 -1.1856671192028827 1103 70-71 -1.1659571439972822 1103 72-73 -0.960441654740098 1103 74-75 -1.0130685395508614 1103 76-77 -1.4506760766066513 1103 78-79 -1.866334948793579 1103 80-81 -0.5899848469162237 1103 82-83 -0.9931831342076158 1103 84-85 -0.06531366661872795 1103 86-87 -0.3980330701593253 1103 88-89 -0.6627687155848996 1103 90-91 -0.8811352948794919 1103 92-93 -1.1641713517787267 1103 94-95 -0.8679391961923066 1103 96-97 -0.9529331199867705 1103 98-99 -0.422846696539402 1103 100-101 -0.32819036455232364 1104 1 -0.007189311337960191 1104 2 0.29521172001260965 1104 3 0.19646265485062742 1104 4 0.10627091995976912 1104 5 0.279489508828064 1104 6 0.20826329231523744 1104 7 0.1180809331629149 1104 8 0.4070808347119339 1104 9 0.34500458809197454 1104 10-11 0.6853076111269303 1104 12-13 0.7323753103663435 1104 14-15 0.7397519347672983 1104 16-17 0.47070378642123245 1104 18-19 0.7978599524415415 1104 20-21 0.6314238744868277 1104 22-23 0.9702695506200811 1104 24-25 0.27049173837064444 1104 26-27 0.5023669848232686 1104 28-29 0.7514537906056518 1104 30-31 0.39517522376725367 1104 32-33 0.905244556449766 1104 34-35 1.1829435899916732 1104 36-37 0.7058357530127424 1104 38-39 1.3283516318954085 1104 40-41 1.0321468519591335 1104 42-43 0.9173754605338544 1104 44-45 1.6821876521761538 1104 46-47 1.3425605181501297 1104 48-49 0.1018015302302615 1104 50-51 -0.9053106314020383 1104 52-53 -0.8853054564891707 1104 54-55 -0.9868638805979622 1104 56-57 -0.17265936984373198 1104 58-59 -0.49339006613728387 1104 60-61 -0.08322720626872027 1104 62-63 -0.46146807032448933 1104 64-65 0.06145135464374718 1104 66-67 -0.4208827047857504 1104 68-69 0.1752024460145094 1104 70-71 -0.4282759845769917 1104 72-73 -1.2283346313287247 1104 74-75 -0.6608946265073818 1104 76-77 -0.2358488748229277 1104 78-79 0.10512547484076151 1104 80-81 -0.18853557155390632 1104 82-83 -0.26943731480962185 1104 84-85 -0.19652882826867213 1104 86-87 -0.017765511630898345 1104 88-89 0.1425824549836605 1104 90-91 0.344394248041354 1104 92-93 1.2796413572513714 1104 94-95 0.7669548952235203 1104 96-97 0.5210914062116672 1104 98-99 -0.6246304200622603 1104 100-101 -0.6436864626571186 1105 1 -0.12313134032346795 1105 2 0.10680592291115687 1105 3 -0.3542619828305291 1105 4 0.19322744169890171 1105 5 0.22151849433531368 1105 6 0.09232126332972967 1105 7 -0.5485857335037494 1105 8 -0.39002061456342574 1105 9 0.4754393707006699 1105 10-11 -0.18425760626437437 1105 12-13 -0.12269715340176646 1105 14-15 0.058592514477439295 1105 16-17 0.15910958352268523 1105 18-19 -0.17315454031207622 1105 20-21 -0.23814134290446987 1105 22-23 -0.5152376957567313 1105 24-25 -1.2874792761221059 1105 26-27 -0.8237199716984733 1105 28-29 -0.9586911369305753 1105 30-31 -0.6410566602907153 1105 32-33 -1.7976539942748744 1105 34-35 -1.3170564100083197 1105 36-37 -1.1637294643785694 1105 38-39 -0.6354164840466154 1105 40-41 -0.6562589451423193 1105 42-43 -1.3724796119299114 1105 44-45 -0.14389930434558096 1105 46-47 0.2338648659762157 1105 48-49 0.2757145737085196 1105 50-51 -0.03574541401073361 1105 52-53 0.6726655580035796 1105 54-55 1.1363245251991465 1105 56-57 0.5447319345040924 1105 58-59 -0.5368683270068502 1105 60-61 -0.2716330033701695 1105 62-63 0.35737250938565523 1105 64-65 -0.0037660366606004914 1105 66-67 1.4994071502867143 1105 68-69 1.0882459242753768 1105 70-71 1.3253472038288052 1105 72-73 1.5035494266422909 1105 74-75 1.6796850836375476 1105 76-77 1.5829917048872169 1105 78-79 1.1703428661451056 1105 80-81 1.4491455878663828 1105 82-83 0.8320119605526983 1105 84-85 1.0570943601371248 1105 86-87 1.243104053586496 1105 88-89 1.4904085419401838 1105 90-91 2.0762783060123695 1105 92-93 0.7216703427586211 1105 94-95 0.7017375039191762 1105 96-97 -0.007894101034711554 1105 98-99 0.686963782836294 1105 100-101 1.588197595313897 1106 1 0.10875271764754402 1106 2 0.19376244465028947 1106 3 -0.13687067848270473 1106 4 -0.08213487714167655 1106 5 0.23601124795850126 1106 6 -0.02362076565577098 1106 7 0.2775012230179925 1106 8 0.23316779123367581 1106 9 0.41746835620791956 1106 10-11 0.23603224880809393 1106 12-13 0.04396951326489784 1106 14-15 0.23975193476729828 1106 16-17 0.10838494584152869 1106 18-19 0.04423676403575172 1106 20-21 0.06620648318248357 1106 22-23 0.0789652027939951 1106 24-25 0.284984491993832 1106 26-27 0.625555390620363 1106 28-29 0.5268161094462371 1106 30-31 1.3734360933324723 1106 32-33 1.369012672391797 1106 34-35 0.6104798218757281 1106 36-37 1.1985893762011415 1106 38-39 1.9805255449388852 1106 40-41 1.2930164171765242 1106 42-43 1.8811435764758855 1106 44-45 2.17494127536456 1106 46-47 2.8498068949617235 1106 48-49 2.4133957331288087 1106 50-51 2.5367183541052043 1106 52-53 3.1364336739456107 1106 54-55 2.542121626648413 1106 56-57 2.6751667171127913 1106 58-59 3.2457403686453183 1106 60-61 3.938511924166061 1106 62-63 3.9805609151827532 1106 64-65 4.249857151745196 1106 66-67 4.484914396663527 1106 68-69 4.689695199637697 1106 70-71 4.34708633426359 1106 72-73 4.74992623823649 1106 74-75 5.0420039242172585 1106 76-77 4.633716342568377 1106 78-79 3.518168953101629 1106 80-81 3.9346528342431952 1106 82-83 3.2160699315671906 1106 84-85 3.520862476079156 1106 86-87 3.1851330390937385 1106 88-89 3.0121476723749687 1106 90-91 2.9965681610848343 1106 92-93 3.2796413572513714 1106 94-95 3.3756505473974343 1106 96-97 3.8399319859218117 1106 98-99 3.3391376958797707 1106 100-101 4.28384976922694 1107 1 0.0458184313660297 1107 2 -0.0929161338686697 1107 3 -0.011200636791222962 1107 4 0.16741842839733323 1107 5 0.2931881389650499 1107 6 0.1163434987873444 1107 7 0.01960962087385809 1107 8 0.3651908208147745 1107 9 0.2326361147943814 1107 10-11 0.3769891676089614 1107 12-13 -0.1960543104396848 1107 14-15 -0.2627297011270855 1107 16-17 0.20040400480519338 1107 18-19 0.13059769316022596 1107 20-21 0.13450110299586981 1107 22-23 -0.3998912593858748 1107 24-25 -0.018172148863818904 1107 26-27 0.2656189204992643 1107 28-29 0.017485158483360408 1107 30-31 -0.36559507601436536 1107 32-33 -0.13386602524568758 1107 34-35 -1.2224564497144996 1107 36-37 -1.20621507089038 1107 38-39 -1.3369253186704029 1107 40-41 -0.6022585480805773 1107 42-43 -0.4534802075225244 1107 44-45 -0.6058806424436582 1107 46-47 -0.44957765933646954 1107 48-49 -1.608938989920624 1107 50-51 -2.3921083284439284 1107 52-53 -1.6495500465229256 1107 54-55 -1.304711805950351 1107 56-57 -1.7054169636495722 1107 58-59 -1.2175314201178296 1107 60-61 -2.3975015759332017 1107 62-63 -2.4543209589486708 1107 64-65 -2.0246117781734085 1107 66-67 -1.8802831415536652 1107 68-69 -1.9623794479700045 1107 70-71 -1.8878749522164568 1107 72-73 -2.0353626241816123 1107 74-75 -2.66238360804401 1107 76-77 -2.010317804741529 1107 78-79 -1.4339851068546956 1107 80-81 -1.53656018938198 1107 82-83 -1.193499256441548 1107 84-85 -1.3065149311076603 1107 86-87 -1.3024587814343498 1107 88-89 -0.8047075986196717 1107 90-91 -0.7424630082619998 1107 92-93 -0.7566897922423728 1107 94-95 -1.297865434337723 1107 96-97 -1.1458730567623263 1107 98-99 -1.2495063382675404 1107 100-101 -0.7670733993257706 1108 1 0.19570923938667661 1108 2 -0.19754190317580012 1108 3 -0.48469676543922446 1108 4 -0.25604792061994175 1108 5 -0.02485831725888943 1108 6 0.3097125676775576 1108 7 -0.09931037118490593 1108 8 0.030269240509035455 1108 9 -0.1187635278500494 1108 10-11 -0.046576446844078134 1108 12-13 0.21063617993156214 1108 14-15 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2316 74-75 -3.8496539054045193 2316 76-77 -3.632101967788639 2316 78-79 -3.053764592320782 2316 80-81 -3.1924238713064703 2316 82-83 -3.136616530080037 2316 84-85 -2.8562142294705097 2316 86-87 -3.2621981733488177 2316 88-89 -3.118021998886963 2316 90-91 -4.646238484372926 2316 92-93 -4.072426511253397 2316 94-95 -3.772723436342403 2316 96-97 -3.67844906745394 2316 98-99 -3.5316647077964376 2316 100-101 -3.1241565934453845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 3.0 5 7.0 6 24.0 7 57.0 8 94.0 9 177.0 10 322.0 11 497.0 12 671.0 13 917.0 14 1024.0 15 1317.0 16 1539.0 17 2008.0 18 1962.0 19 1395.0 20 1312.0 21 1372.0 22 1450.0 23 1462.0 24 1579.0 25 1578.0 26 1225.0 27 1113.0 28 1345.0 29 1484.0 30 1752.0 31 2125.0 32 2398.0 33 2905.0 34 3899.0 35 5302.0 36 7291.0 37 10822.0 38 9175.0 39 942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.58742961497489 21.543697513869482 16.029108617755703 21.839764253399927 2 15.268722345513943 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28.07115485347228 33.27888512116451 20.859868221542193 46-47 20.325035150111653 26.1289388801588 31.27532875692664 22.27069721280291 48-49 19.68723723930693 26.347057769446014 31.25628902642425 22.709415964822803 50-51 18.583125422140128 27.396032916592 31.897942023791476 22.122899637476394 52-53 18.633437633534122 27.955670117303267 33.22604656291783 20.184845686244778 54-55 17.985581760789557 28.33128868182006 31.10535239224227 22.57777716514811 56-57 20.0373550939391 29.309964574689513 28.730340331095704 21.922340000275682 58-59 17.72161495306491 30.676664782830443 30.947523674307693 20.654196589796957 60-61 18.774854748333826 32.791382079080314 27.663636426292104 20.77012674629376 62-63 17.512784815361076 33.6643830895833 27.84332915213586 20.979502942919762 64-65 17.87944367099949 33.33011702758212 27.902601072408235 20.887838229010157 66-67 17.778375099936593 32.76817467537838 27.936068149863537 21.51738207482149 68-69 17.831888293106537 31.875198147407886 27.959805367554825 22.333108191930748 70-71 18.405690221998608 30.919216216029938 27.734318462206474 22.940775099764977 72-73 18.38670103519098 30.07567507960357 27.81507160875019 23.722552276455264 74-75 18.718899472066386 30.0405254524653 27.365018539705293 23.875556535763025 76-77 18.62427219294131 29.761444852231023 28.093352465575762 23.520930489251914 78-79 18.287309959664906 29.299824180370255 28.272485951666837 24.140379908297994 80-81 18.157194646229343 29.694542848084687 27.764070188980938 24.384192316705033 82-83 18.433567204708673 29.20244806814892 27.50010338125629 24.863881345886117 84-85 18.508001709236773 28.901264008160226 27.451858794988077 25.138875487614925 86-87 18.7310177259926 28.72466729250649 27.243359655419958 25.300955326080953 88-89 18.77796294887664 28.154151482252388 27.45434303061316 25.61354253825781 90-91 18.61633742068374 28.22345411062112 27.27995355074167 25.880254917953465 92-93 18.643775759163024 28.292693012805493 27.127241650240535 25.93628957779095 94-95 18.530056377245096 28.099025459357378 27.11621431623637 26.25470384716115 96-97 18.989758363543636 27.807490316622328 26.48834548637435 26.714405833459686 98-99 18.527299543744054 27.89157373840407 26.869477717893226 26.711648999958648 100-101 18.946338235902243 27.81576081712545 27.34709912194853 25.89080182502378 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 224.0 1 147.0 2 80.5 3 95.5 4 89.0 5 89.5 6 128.5 7 189.0 8 262.0 9 307.5 10 272.0 11 227.0 12 231.0 13 239.0 14 255.0 15 246.0 16 237.5 17 287.5 18 322.5 19 329.0 20 350.0 21 383.5 22 439.5 23 496.0 24 566.5 25 639.5 26 739.0 27 886.0 28 976.0 29 1049.0 30 1156.5 31 1249.0 32 1333.5 33 1393.0 34 1481.5 35 1595.0 36 1661.0 37 1698.5 38 1770.0 39 1852.5 40 1857.5 41 1996.5 42 2237.0 43 2421.5 44 2619.5 45 2736.0 46 2823.0 47 2896.5 48 2883.5 49 2780.5 50 2692.0 51 2572.5 52 2256.0 53 1974.0 54 1760.0 55 1554.0 56 1396.0 57 1226.5 58 1100.0 59 1002.5 60 854.5 61 682.0 62 539.5 63 428.0 64 314.0 65 219.0 66 168.5 67 138.0 68 116.0 69 88.0 70 66.0 71 56.0 72 44.5 73 43.0 74 36.0 75 25.0 76 23.5 77 17.0 78 10.0 79 8.0 80 4.0 81 3.0 82 5.5 83 4.0 84 2.0 85 2.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3666588556384137 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0013784167505203524 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010338125628902642 36-37 0.018608626132024755 38-39 0.01102733400416282 40-41 0.007581292127861938 42-43 0.0020676251257805284 44-45 0.0013784167505203524 46-47 0.0013784167505203524 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 6.892083752601762E-4 62-63 0.0 64-65 0.0 66-67 0.0013784167505203524 68-69 0.0 70-71 0.0020676251257805284 72-73 0.0 74-75 0.0 76-77 0.0951107557859043 78-79 0.040663294140350394 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1530042593077591 88-89 0.33288764525066505 90-91 0.28946751760927397 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 72547.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.72672887920933 #Duplication Level Percentage of deduplicated Percentage of total 1 91.49819228215156 68.37360607606104 2 5.762561794436656 8.61234785725116 3 1.2912270346048846 2.89467517609274 4 0.49804471334759837 1.4886900905619804 5 0.2730022873164613 1.0200283953850606 6 0.18077178484468384 0.8105090493059672 7 0.11436582306500406 0.598232869725833 8 0.06456135173024423 0.3859566901456986 9 0.044270641186453186 0.2977380181123961 >10 0.2213532059322659 3.113843439425476 >50 0.023979930642662142 1.2350614084662357 >100 0.018446100494355494 3.5659641335961516 >500 0.005533830148306648 2.9553255131156355 >1k 0.0036892200988710984 4.648021282754629 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2125 2.9291355948557487 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1247 1.7188856878988794 RNA PCR Primer, Index 17 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 792 1.091706066412119 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 738 1.01727156188402 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 614 0.8463478848194964 RNA PCR Primer, Index 17 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 487 0.6712889575034116 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 464 0.6395853722414435 RNA PCR Primer, Index 17 (96% over 28bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 360 0.49623003018732686 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 253 0.34873943788164913 RNA PCR Primer, Index 17 (96% over 26bp) TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 204 0.28119701710615186 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.2632775993493873 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.23984451459054132 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 171 0.23570926433898026 TruSeq Adapter, Index 12 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.19987042882545109 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 138 0.1902215115718086 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 93 0.12819275779839276 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA 88 0.12130067404579101 No Hit ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC 86 0.11854384054475031 TruSeq Adapter, Index 25 (95% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.11027334004162818 No Hit CTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 77 0.10613808979006714 No Hit CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA 76 0.10475967303954677 RNA PCR Primer, Index 17 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.892083752601762E-4 0.0 20-21 0.0 0.0 0.0 0.003446041876300881 0.0 22-23 0.0 0.0 0.0 0.01998704288254511 0.0 24-25 0.0 0.0 0.0 0.03997408576509022 0.0 26-27 0.0 0.0 0.0 0.06685321240023709 0.0 28-29 0.0 0.0 0.0 0.15300425930775913 0.0 30-31 0.0 0.0 0.0 0.2984272264876563 0.0 32-33 0.0 0.0 0.0 0.5134602395688312 0.0 34-35 0.0 0.0 0.0 0.8670241360773017 0.0 36-37 0.0 0.0 0.0 1.3763491253945719 0.0 38-39 0.0 0.0 0.0 1.8808496560850207 0.0 40-41 0.0 0.0 0.0 2.3708768108950062 0.0 42-43 0.0 0.0 0.0 2.802321253807876 0.0 44-45 0.0 0.0 0.0 3.3481742870139355 0.0 46-47 0.0 0.0 0.0 3.9126359463520197 0.0 48-49 0.0 0.0 0.0 4.448840062304438 0.0 50-51 0.0 0.0 0.0 4.965746343749569 0.0 52-53 0.0 0.0 0.0 5.4068397039160825 0.0 54-55 0.0 0.0 0.0 5.94924669524584 0.0 56-57 0.0 0.0 0.0 6.588832067487284 0.0 58-59 0.0 0.0 0.0 7.220836147600865 0.0 60-61 0.0 0.0 0.0 7.888679063227976 0.0 62-63 0.0 0.0 0.0 8.542737811349884 0.0 64-65 0.0 0.0 0.0 9.236081436861621 0.0 66-67 0.0 0.0 0.0 9.972845190014748 0.0 68-69 0.0 0.0 0.0 10.744069361930887 0.0 70-71 0.0 0.0 0.0 11.560092078238934 0.0 72-73 0.0 0.0 0.0 12.391277378802707 0.0 74-75 0.0 0.0 0.0 13.245206555750066 0.0 76-77 0.0 0.0 0.0 14.117055150454188 0.0 78-79 0.0 0.0 0.0 15.098487876824679 0.0 80-81 0.0 0.0 0.0 16.095083187450896 0.0 82-83 0.0 0.0 0.0 17.082029580823466 0.0 84-85 0.0 0.0 0.0 18.088963017078584 0.0 86-87 0.0 0.0 0.0 19.11588349621625 0.0 88-89 0.0 0.0 0.0 20.167615476863276 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGAAC 25 0.005021099 56.94621 4 CTAACCT 35 2.783462E-4 54.234486 4 CACTGAC 60 0.003944986 31.636784 7 TCCCGAC 60 0.003944986 31.636784 7 CATGGGC 110 2.7070182E-6 30.198746 4 ATGGGCT 65 0.0058353613 29.203184 5 GGTATCA 595 0.0 28.831678 1 GTACATG 465 0.0 27.669107 1 CTCCCGA 70 0.008379222 27.117243 6 GTATCAA 805 0.0 26.637962 1 TACATGG 470 0.0 26.251799 2 GTTTCTG 60 1.7117156E-4 23.727589 16-17 ACATGGG 480 0.0 23.727589 3 AGTACTT 575 0.0 22.283298 12-13 CATGGGG 130 1.2148115E-4 21.902388 4 TATCAAC 980 0.0 21.790642 2 TCAACGC 985 0.0 21.680029 4 AACGCAG 1000 0.0 21.354828 6 ATCAACG 1005 0.0 21.248585 3 CAACGCA 1020 0.0 20.936106 5 >>END_MODULE