FastQCFastQC Report
Thu 26 May 2016
SRR938848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72544
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8131.1206991618879578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7681.05866784296427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.6037715041905602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.39975738861932075No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.3556462284958094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.23709748566387295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.21917732686369654No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC1000.13784737538597264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.12268416409351565No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.1088994265549184No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG200.002054017571.35611
GTATTAT352.750123E-454.3665541
ACCCATG601.4858815E-647.4391483
GTACCCA554.8739843E-543.2461241
TACCCAT554.9544527E-543.126492
TATTATA500.001615964337.9513132
ATGGGGC651.3258748E-436.491655
CATGGGG1255.5679266E-934.1561854
GTATCAT352.0515922E-433.90855454-55
GAGTAAA352.0557693E-433.89682412-13
CGCCGTA300.00395102831.64798250-51
GGTATCA8200.030.1668071
ATGGGGG856.396612E-427.9053785
CCGTATC350.00839186227.12684252-53
GCCGTAT350.00839186227.12684250-51
CAGCAGG700.00839289127.1080829
AGAGTAG458.9403446E-426.37331810-11
GTATCAA10600.024.2341481
GTACATG5900.024.1885091
GAGTACC701.7783985E-523.72777712-13
Produced by FastQC (version 0.11.5)