FastQCFastQC Report
Thu 26 May 2016
SRR938848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72544
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT20642.845169827966475No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT11441.5769739744155271RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8141.1220776356418174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7661.0559108954565506No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT6280.8656815174239083RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG5420.7471327745919717RNA PCR Primer, Index 17 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.6561535068372298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.5210630789589766No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT2660.36667401852668724RNA PCR Primer, Index 17 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.3032642258491398No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC2130.2936149095721217No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG2020.27845169827966476TruSeq Adapter, Index 12 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.25226069695632997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.21228495809439787No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT1480.20401411557123952No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA1080.14887516541685047RNA PCR Primer, Index 17 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC870.1199272165857962No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1199272165857962No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA760.10476400529333921No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT200.00207343871.1874163
GTACCCA250.00497877657.0680051
TGCGGGG250.001569353238.2037590-91
CCTGGAC550.002574855234.515113
GGTATCA7600.034.4160081
CTTGCAA300.00395665931.6388556-57
GAGTACC404.4975986E-429.66142312-13
GTATCAA9400.027.7681392
CTAACAT350.00840377727.11901594-95
CATGGGG1551.3910503E-624.4945934
TATCAAC10700.024.3944423
ATCAACG11050.024.0512524
AACGCAG10950.023.837497
TCAACGC11250.023.6236745
CAACGCA11500.023.1101196
TACATGG4950.023.0100732
GTACATG5000.022.8272021
ACATGGG4850.021.5274643
GAGTACT6450.020.60204312-13
CGCAGAG12650.020.258879
Produced by FastQC (version 0.11.5)