##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938848_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72544 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.794056021173358 31.0 31.0 34.0 30.0 34.0 2 32.028341420379355 33.0 31.0 34.0 30.0 34.0 3 32.04464876488752 33.0 31.0 34.0 30.0 34.0 4 35.75587229819144 37.0 35.0 37.0 33.0 37.0 5 35.66527900308778 37.0 35.0 37.0 33.0 37.0 6 35.702759704455225 37.0 35.0 37.0 33.0 37.0 7 35.57753914865461 37.0 35.0 37.0 33.0 37.0 8 35.596341530657256 37.0 35.0 37.0 33.0 37.0 9 37.12362152624614 39.0 37.0 39.0 33.0 39.0 10-11 37.10364744155271 39.0 37.0 39.0 33.0 39.0 12-13 37.00769188354654 39.0 37.0 39.0 33.0 39.0 14-15 38.07418945743273 40.0 37.0 41.0 33.0 41.0 16-17 37.97690367225408 40.0 37.0 41.0 33.0 41.0 18-19 38.15268664534627 40.0 37.0 41.0 33.0 41.0 20-21 38.19133215703573 40.0 37.0 41.0 33.0 41.0 22-23 38.08444530216145 40.0 37.0 41.0 33.0 41.0 24-25 37.94251075209528 40.0 37.0 41.0 33.0 41.0 26-27 37.60233099911778 40.0 36.5 41.0 32.0 41.0 28-29 37.28097430524923 39.5 36.0 41.0 31.5 41.0 30-31 36.81871002426114 39.0 35.0 41.0 31.0 41.0 32-33 36.40895180855757 39.0 35.0 41.0 30.0 41.0 34-35 36.112324933833264 38.5 35.0 41.0 30.0 41.0 36-37 35.77284820247023 38.0 35.0 41.0 29.5 41.0 38-39 35.281070798412 38.0 35.0 41.0 26.5 41.0 40-41 34.93100738861932 38.0 35.0 40.0 25.0 41.0 42-43 34.50340483017203 38.0 34.0 40.0 23.5 41.0 44-45 33.94822452580503 38.0 33.5 40.0 19.5 41.0 46-47 33.15715979267755 38.0 33.0 40.0 5.5 41.0 48-49 32.52690780767534 38.0 32.5 40.0 2.0 41.0 50-51 31.904575154389057 37.5 31.0 40.0 2.0 41.0 52-53 31.558295655050728 37.0 31.0 40.0 2.0 41.0 54-55 31.146359450816057 37.0 31.0 40.0 2.0 41.0 56-57 30.556889611821788 36.0 30.0 40.0 2.0 41.0 58-59 29.850015163211292 35.5 28.5 39.5 2.0 41.0 60-61 29.087278065725627 35.0 26.5 39.0 2.0 41.0 62-63 28.387626819585357 35.0 25.0 39.0 2.0 40.0 64-65 27.659441166740187 35.0 21.0 38.5 2.0 40.0 66-67 26.893664534627263 34.0 13.0 38.0 2.0 40.0 68-69 26.38165113586237 34.0 4.0 37.0 2.0 40.0 70-71 25.932413431848257 34.0 2.0 37.0 2.0 39.0 72-73 25.46788845390384 34.0 2.0 36.0 2.0 39.0 74-75 25.01766514115571 33.0 2.0 35.5 2.0 38.5 76-77 23.36918973312748 30.5 2.0 34.0 2.0 36.0 78-79 24.033152293780326 32.5 2.0 35.0 2.0 37.0 80-81 24.022896449051608 33.0 2.0 35.0 2.0 36.5 82-83 23.72063161667402 33.0 2.0 35.0 2.0 36.0 84-85 23.333949051610055 32.0 2.0 35.0 2.0 36.0 86-87 22.81234147551831 31.5 2.0 35.0 2.0 35.0 88-89 22.282221823996473 31.0 2.0 35.0 2.0 35.0 90-91 21.832991012351126 31.0 2.0 34.0 2.0 35.0 92-93 21.54028589545655 30.5 2.0 34.0 2.0 35.0 94-95 21.251985002205558 30.0 2.0 34.0 2.0 35.0 96-97 20.676134483899425 30.0 2.0 34.0 2.0 35.0 98-99 19.993238586237318 29.0 2.0 34.0 2.0 35.0 100-101 17.473740074988974 22.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.9805865651135548 1101 2 -0.32837875864971267 1101 3 -0.5611805996784938 1101 4 -0.31635867078335167 1101 5 -1.372998506163924 1101 6 -1.5354065047519256 1101 7 -1.0329236087205942 1101 8 -0.5264725049330607 1101 9 -0.5710248586501621 1101 10-11 -1.225051558507353 1101 12-13 -0.7346610244623335 1101 14-15 -0.4123576077328224 1101 16-17 -0.21872287635960674 1101 18-19 -0.5247136391676079 1101 20-21 -0.5819487491177568 1101 22-23 -0.5075572848399901 1101 24-25 -0.30619297150312264 1101 26-27 -1.420138324551118 1101 28-29 -1.0658772801494862 1101 30-31 -0.9879725892380051 1101 32-33 -0.2948104219274512 1101 34-35 0.20513157188652542 1101 36-37 -0.843879890131042 1101 38-39 -0.8483091603661208 1101 40-41 -0.9936756198016994 1101 42-43 -0.8870051927460025 1101 44-45 -1.1553831430456185 1101 46-47 -1.421110858340743 1101 48-49 -3.401412451242244 1101 50-51 -3.3075880111004423 1101 52-53 -3.1329216549741794 1101 54-55 -2.5152368030075927 1101 56-57 -1.7967850856275795 1101 58-59 -2.629886415278591 1101 60-61 -1.6071693063675276 1101 62-63 -1.8575112646460283 1101 64-65 -2.3707410021494475 1101 66-67 -2.0969792403419945 1101 68-69 -1.6946159193536694 1101 70-71 -1.3733518212356977 1101 72-73 -1.9648552814892533 1101 74-75 -2.3340652447486328 1101 76-77 -2.131595197846739 1101 78-79 -1.6193978746280386 1101 80-81 -1.0991148041510321 1101 82-83 -1.0064980371348966 1101 84-85 -1.6671644552504432 1101 86-87 -2.225810569277172 1101 88-89 -2.1716814072110573 1101 90-91 -2.9395963085659247 1101 92-93 -2.3657702993435343 1101 94-95 -2.1778019590139017 1101 96-97 -1.8394345666986176 1101 98-99 -2.50420254336294 1101 100-101 -2.313882532531874 1102 1 0.41994044410910547 1102 2 0.1894078026151078 1102 3 0.23394456501188898 1102 4 0.25017624357764845 1102 5 0.46033482716940455 1102 6 -0.3147213927624719 1102 7 -0.46243612518962607 1102 8 -0.28075445486718564 1102 9 -0.13492472689785728 1102 10-11 -0.28928080751920504 1102 12-13 0.15203199266547074 1102 14-15 -0.3105789516063382 1102 16-17 0.43246289439137797 1102 18-19 0.5517026981182909 1102 20-21 -0.13530843291222538 1102 22-23 0.13308830145776795 1102 24-25 -0.04170021787993505 1102 26-27 0.7030500812459834 1102 28-29 0.8096167909967633 1102 30-31 0.4234239852020423 1102 32-33 -0.012860487803607157 1102 34-35 0.8457771581842763 1102 36-37 0.5454481731100671 1102 38-39 1.2514932111753794 1102 40-41 0.7342558689993552 1102 42-43 1.159108114236865 1102 44-45 1.1147090835683429 1102 46-47 -0.5890950480640598 1102 48-49 0.3456231218802799 1102 50-51 -0.4762309623520906 1102 52-53 -1.0798913519438749 1102 54-55 -0.6394133510971862 1102 56-57 -0.960487325416775 1102 58-59 -0.5607164548042824 1102 60-61 -1.1921495435216798 1102 62-63 -0.815021146068954 1102 64-65 -0.6988042432561663 1102 66-67 -0.21654445773330266 1102 68-69 -0.38598614333259107 1102 70-71 0.042326703138478194 1102 72-73 -0.38843894420335445 1102 74-75 -0.19177275463005472 1102 76-77 -0.23831456543566887 1102 78-79 0.520918330905559 1102 80-81 0.6778944185103661 1102 82-83 0.7461370089784118 1102 84-85 0.6836260585835525 1102 86-87 0.7524503002880465 1102 88-89 1.4202158259905246 1102 90-91 0.7702192382061455 1102 92-93 0.6819899114601533 1102 94-95 -0.1175252791720034 1102 96-97 -0.41222771557872306 1102 98-99 0.5158896832510251 1102 100-101 -0.2799563138230461 1103 1 0.2368047392342696 1103 2 -0.009538178939571651 1103 3 -0.05393422286689997 1103 4 0.1184239379122971 1103 5 -0.06865068007697062 1103 6 0.015318132929230899 1103 7 -0.16335839132928953 1103 8 -0.10618264986059955 1103 9 0.06665630077012707 1103 10-11 0.07929626757960762 1103 12-13 0.10591868568259599 1103 14-15 0.5586568850208025 1103 16-17 0.2377988627708234 1103 18-19 -0.27833682757340483 1103 20-21 -0.6689052708568965 1103 22-23 -0.5003109080284034 1103 24-25 -0.0815552903437009 1103 26-27 0.18855732762278876 1103 28-29 -0.036891772903111075 1103 30-31 0.004781033950393976 1103 32-33 0.6182330563334162 1103 34-35 0.7123779486981192 1103 36-37 0.43872880552113713 1103 38-39 0.6516908396338792 1103 40-41 0.6367591628070031 1103 42-43 0.7216904594279043 1103 44-45 1.6054864221717722 1103 46-47 2.6151210257172224 1103 48-49 2.7797469690476078 1103 50-51 2.888064162812597 1103 52-53 1.7366435624171253 1103 54-55 1.8253429071373368 1103 56-57 2.282925059299952 1103 58-59 2.0150411209532955 1103 60-61 2.4145698240672537 1103 62-63 2.2946626483974484 1103 64-65 3.1437517514737436 1103 66-67 2.9320062669043807 1103 68-69 2.232920312530389 1103 70-71 2.9092568744164744 1103 72-73 2.411956312713645 1103 74-75 2.789123161048469 1103 76-77 2.426375816646015 1103 78-79 2.351616618125586 1103 80-81 2.3791460654141865 1103 82-83 2.863067180256408 1103 84-85 3.071965979532166 1103 86-87 2.7234647930416713 1103 88-89 2.371796853658509 1103 90-91 1.9372152856369738 1103 92-93 2.2356789760187823 1103 94-95 1.9308936931600122 1103 96-97 2.7982465927216715 1103 98-99 2.8943481812747436 1103 100-101 3.468726163120298 1104 1 0.5317628939597263 1104 2 0.4962793348387535 1104 3 0.4671925376964836 1104 4 0.4452253259506733 1104 5 0.5753583013478121 1104 6 0.4311172756114132 1104 7 0.3036756972602177 1104 8 0.6123313325847946 1104 9 0.5530821019540397 1104 10-11 0.5166304174061054 1104 12-13 0.05856208229415216 1104 14-15 0.728793647625416 1104 16-17 0.694626786836956 1104 18-19 0.4842677856129569 1104 20-21 0.5544056089144931 1104 22-23 -0.256689760855501 1104 24-25 0.18952043939706442 1104 26-27 0.2570406915746162 1104 28-29 0.37747850674579553 1104 30-31 -0.48470661659053604 1104 32-33 0.5022910273479084 1104 34-35 0.8550601287348556 1104 36-37 0.4359731410998293 1104 38-39 0.08749406478187183 1104 40-41 -1.1138021762417836 1104 42-43 -0.9343617961344535 1104 44-45 -0.37910226939794 1104 46-47 -0.9226417830192517 1104 48-49 -1.8268053885763962 1104 50-51 -1.6631707830947278 1104 52-53 -1.0484146127206593 1104 54-55 -1.7786579093558252 1104 56-57 -1.077964918246483 1104 58-59 -1.1422358743518721 1104 60-61 -1.2681205209011068 1104 62-63 -0.6772703991632767 1104 64-65 -0.24489899357626754 1104 66-67 -0.7083284952919868 1104 68-69 -0.43752263500992683 1104 70-71 -0.11921832460373238 1104 72-73 0.18731863155422346 1104 74-75 -0.17811505083151147 1104 76-77 -0.08658601229866392 1104 78-79 0.18331696513518025 1104 80-81 0.013255067251296992 1104 82-83 -0.35320144599385017 1104 84-85 -0.478452473213288 1104 86-87 -0.14895815041617766 1104 88-89 0.24319918066402124 1104 90-91 0.22145696761288747 1104 92-93 0.32294168269361734 1104 94-95 0.11113455864276389 1104 96-97 -0.21216369509216904 1104 98-99 -0.12228786689835403 1104 100-101 0.608754740380963 1105 1 -0.20394547389445705 1105 2 0.3440423837202786 1105 3 -0.568640105219842 1105 4 -0.2654209043724407 1105 5 -1.0739788983207887 1105 6 -0.032635831265146464 1105 7 0.4751752830100173 1105 8 0.26871078577452323 1105 9 0.23183106632852457 1105 10-11 0.5311291746554758 1105 12-13 0.3879945595103962 1105 14-15 0.14262960454339435 1105 16-17 0.028719578883361407 1105 18-19 0.3455975287778301 1105 20-21 0.9351015492624555 1105 22-23 0.8541221695504575 1105 24-25 1.2916331154534006 1105 26-27 1.0755991008538217 1105 28-29 1.1217825663978331 1105 30-31 1.1867929691592636 1105 32-33 1.2375851449949664 1105 34-35 0.9834776929436373 1105 36-37 0.4364909538587298 1105 38-39 1.159470038269852 1105 40-41 1.197713979516287 1105 42-43 1.2186000928464935 1105 44-45 0.7849408126236028 1105 46-47 0.303953080278184 1105 48-49 -0.44403819719279625 1105 50-51 0.35598828898481116 1105 52-53 1.0803136391434656 1105 54-55 0.28037274515950017 1105 56-57 -0.05072796712800809 1105 58-59 -0.25946868296827574 1105 60-61 0.3996508129845573 1105 62-63 -0.142784069420113 1105 64-65 0.4201797139630834 1105 66-67 0.0898280912863072 1105 68-69 0.09396038073158053 1105 70-71 -0.22661269080091628 1105 72-73 0.3343774550836365 1105 74-75 0.22422546283874922 1105 76-77 0.0029955949921394165 1105 78-79 0.024037760495577487 1105 80-81 -0.07321966348606779 1105 82-83 -0.255645522215886 1105 84-85 -0.2546537988139548 1105 86-87 0.2671561826409885 1105 88-89 0.33599122706004536 1105 90-91 0.47632440754660976 1105 92-93 0.7305638865047186 1105 94-95 0.8441503001506341 1105 96-97 0.6713284341538959 1105 98-99 -0.027113881811359875 1105 100-101 0.6309171264621583 1106 1 -0.16360933944067568 1106 2 -0.0530164398091344 1106 3 -0.3539342228669007 1106 4 -0.23726964386823823 1106 5 -0.24918898235440423 1106 6 -0.5380980161390951 1106 7 -0.11221967497318275 1106 8 -0.04179341590614882 1106 9 -0.40808490005802867 1106 10-11 -0.3596271278655294 1106 12-13 -0.6323835917501128 1106 14-15 -0.23720232822972065 1106 16-17 -0.7090955471670597 1106 18-19 -0.5273016308860363 1106 20-21 -0.027083324687126265 1106 22-23 -0.0017601833907150422 1106 24-25 -0.2726525988323161 1106 26-27 -0.008854680658785696 1106 28-29 0.17325315463312307 1106 30-31 0.2493980111760692 1106 32-33 0.038005313062193125 1106 34-35 0.59188105428818 1106 36-37 0.140902718564611 1106 38-39 0.33699104667321933 1106 40-41 0.9775459122893935 1106 42-43 0.5104068155355677 1106 44-45 0.452587871447129 1106 46-47 1.0245413155723 1106 48-49 1.1399954162525745 1106 50-51 1.576366440245316 1106 52-53 0.6242212021686733 1106 54-55 0.7118853502015163 1106 56-57 -0.32152628645573955 1106 58-59 0.688850644762816 1106 60-61 -0.26379456516670174 1106 62-63 0.7196108885630821 1106 64-65 0.7823645879126637 1106 66-67 0.14297935179050825 1106 68-69 0.3250528177063714 1106 70-71 0.012673023484797596 1106 72-73 -0.03410993987434807 1106 74-75 0.3807380678807668 1106 76-77 0.45908803196692816 1106 78-79 -0.2873067773195501 1106 80-81 -0.6242700836541353 1106 82-83 0.04267380551520361 1106 84-85 -0.239317664360172 1106 86-87 0.8238788717166194 1106 88-89 0.013722319497016855 1106 90-91 0.06350928149618795 1106 92-93 0.2620764915467362 1106 94-95 0.03658727494054759 1106 96-97 0.8501099467589377 1106 98-99 1.303768471129814 1106 100-101 1.432597798731063 1107 1 0.07546629081142342 1107 2 0.16757179548498158 1107 3 0.22547754183898405 1107 4 0.29340262503932024 1107 5 0.2348446310909722 1107 6 0.3938347569701506 1107 7 0.0781164594806043 1107 8 0.16576960930393625 1107 9 0.46712518397558256 1107 10-11 0.06054093936135985 1107 12-13 0.3071122065692151 1107 14-15 0.5838060751316263 1107 16-17 0.5654842847657093 1107 18-19 0.345597528777823 1107 20-21 0.3542191963212815 1107 22-23 0.8247104048445735 1107 24-25 0.7989860566298717 1107 26-27 0.6123638067361767 1107 28-29 0.6953119781625361 1107 30-31 0.6353223809239665 1107 32-33 0.9361145567596765 1107 34-35 0.6746541635318692 1107 36-37 0.34090271856461385 1107 38-39 -0.009647608788966977 1107 40-41 -0.19934484401311892 1107 42-43 -0.4137528483299775 1107 44-45 -0.40623565796463623 1107 46-47 0.392188374395829 1107 48-49 0.7471382733954357 1107 50-51 0.34863534780834016 1107 52-53 0.19060775679052355 1107 54-55 0.13331392163008715 1107 56-57 -0.12425737889271105 1107 58-59 0.07876661114937278 1107 60-61 0.3702390482786768 1107 62-63 0.3278041658740065 1107 64-65 0.5819444198454384 1107 66-67 0.41335750305101016 1107 68-69 1.1160192042609935 1107 70-71 1.3248578974343772 1107 72-73 1.8932009844953974 1107 74-75 1.5109901687211007 1107 76-77 1.0544661832274294 1107 78-79 2.0755083487308674 1107 80-81 2.000309748278635 1107 82-83 1.862001536607643 1107 84-85 1.0909344364801647 1107 86-87 0.9509797120527494 1107 88-89 0.6521676976482773 1107 90-91 1.3880891134289612 1107 92-93 0.6937991806223636 1107 94-95 0.800032653091808 1107 96-97 1.4213284341538959 1107 98-99 0.03170964760040107 1107 100-101 0.27062300881510026 1108 1 0.09187720300238666 1108 2 -0.4443207876352204 1108 3 -0.06842697649008755 1108 4 -0.2149093954210315 1108 5 -0.0831434337001582 1108 6 0.47908624887126194 1108 7 0.34388798548230426 1108 8 0.2561361907191113 1108 9 -0.0058074673458179404 1108 10-11 -0.7395443121305405 1108 12-13 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2316 72-73 2.7114150170963924 2316 74-75 2.400961820032226 2316 76-77 1.963218840917012 2316 78-79 2.590834358652913 2316 80-81 2.236401172800253 2316 82-83 2.0379405869265668 2316 84-85 2.1495807865864727 2316 86-87 1.957269577396481 2316 88-89 1.6620011491011475 2316 90-91 1.3922528271072032 2316 92-93 1.9148303641801263 2316 94-95 1.3836612852675714 2316 96-97 1.145548845209884 2316 98-99 1.8067805193225865 2316 100-101 0.21684908960886062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 0.0 5 11.0 6 21.0 7 56.0 8 119.0 9 196.0 10 329.0 11 499.0 12 686.0 13 901.0 14 1091.0 15 1325.0 16 1600.0 17 2077.0 18 2024.0 19 1467.0 20 1308.0 21 1406.0 22 1412.0 23 1383.0 24 1538.0 25 1550.0 26 1279.0 27 1181.0 28 1323.0 29 1494.0 30 1813.0 31 2047.0 32 2489.0 33 2964.0 34 3903.0 35 5466.0 36 7563.0 37 10831.0 38 8388.0 39 802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2785261325486 21.862090880203368 15.951700031776294 21.907682955471742 2 15.307951036612263 21.954951477723863 40.46509704455227 22.2720004411116 3 17.87466916629907 28.47926775474195 34.596934274371414 19.04912880458756 4 12.380072783414203 22.263729598588444 32.97584913983238 32.380348478164976 5 11.25589476296848 39.44182454979179 34.01450594302419 15.287774744215548 6 25.68962902714402 37.6969630129999 17.74907291250224 18.864335047353837 7 21.471107190119103 37.87494486104985 19.681848257609175 20.972099691221878 8 24.543725187472432 34.117225408028226 21.617225408028233 19.72182399647111 9 23.74007498897221 16.875275694750773 20.61093956771063 38.77370974856639 10-11 22.558722981914425 27.93752756947508 26.907118438464934 22.59663101014557 12-13 25.49831826202029 25.540361711513015 27.235195191883548 21.72612483458315 14-15 23.13561424790472 27.013260917512135 26.186176665196292 23.664948169386854 16-17 20.35855090843925 31.057180667751094 27.048468473436078 21.535799950373576 18-19 19.535730039700045 27.20142258491398 31.526383987648877 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18.07216459316952 28.0690629630906 33.14884378123169 20.709928662508183 46-47 20.526304906711147 25.982341629505058 31.0772157395218 22.414137724261995 48-49 19.71933887487421 26.36505245164937 31.23044263402396 22.68516603945246 50-51 18.85131782090869 27.40474746360829 31.822755844728718 21.9211788707543 52-53 18.615598808998676 27.86584693427437 33.20467578297309 20.31387847375386 54-55 17.9815008822232 28.43033193647993 31.123180414644906 22.46498676665196 56-57 19.8570512864695 29.749736365077506 28.409850641339336 21.983361707113662 58-59 17.697535288928098 30.853688795765326 30.937775694750773 20.5110002205558 60-61 18.99149469969535 32.635815998786924 27.784210743972544 20.58847855754518 62-63 17.850545875606528 33.76985002205558 27.469535730039702 20.91006837229819 64-65 17.957625132679926 33.079690666225545 27.992363150131645 20.970321050962877 66-67 17.979789624605374 32.52546976026028 27.866468147290348 21.628272467844 68-69 17.97943317159241 32.19149757388619 27.76935377150419 22.059715483017204 70-71 18.4129631671813 31.023930304367003 27.57912439347155 22.98398213498015 72-73 18.35093184825761 30.55938464931628 27.339269960299955 23.750413542126157 74-75 18.686590207322453 30.325733348037055 27.24966916629907 23.73800727834142 76-77 18.63417863817872 29.94889549438954 27.72884956240474 23.688076305027 78-79 18.427937349430856 29.4166373294125 28.197588132562974 23.957837188593675 80-81 18.2999283193648 29.857741508601677 27.58325981473313 24.2590703573004 82-83 18.59905712395236 29.19331715924129 27.541216365240405 24.666409351565946 84-85 18.577690780767533 28.86386193206881 27.323417512130572 25.235029775033084 86-87 18.83408071748879 28.620213866850637 27.315626077957916 25.230079337702655 88-89 18.98813846526265 28.308607393574714 27.263547394266347 25.43970674689629 90-91 18.51759610927782 28.27691647814928 27.57473244366962 25.63075496890328 92-93 18.62675498149386 28.285787148400615 27.163012537305203 25.92444533280032 94-95 18.510834803705336 28.168421923246584 27.138702029113364 26.182041243934716 96-97 18.660399205999116 28.014032862814293 26.71757829730922 26.60798963387737 98-99 18.60526025584473 27.82173577415086 26.796151301279224 26.776852668725187 100-101 18.84718239964711 27.631506396118215 27.30756506396118 26.213746140273493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 238.0 1 166.0 2 102.0 3 99.0 4 88.0 5 88.5 6 115.5 7 180.5 8 251.0 9 291.0 10 262.0 11 222.0 12 225.5 13 239.5 14 247.0 15 257.0 16 276.5 17 292.5 18 303.0 19 316.0 20 353.0 21 399.0 22 423.5 23 497.0 24 591.5 25 665.0 26 743.0 27 836.5 28 928.0 29 1024.0 30 1121.0 31 1195.0 32 1324.0 33 1441.0 34 1549.0 35 1587.5 36 1601.5 37 1666.5 38 1771.5 39 1815.5 40 1855.0 41 2021.5 42 2214.0 43 2421.5 44 2643.5 45 2826.0 46 2899.0 47 2892.5 48 2850.5 49 2841.0 50 2763.0 51 2520.5 52 2222.5 53 1987.0 54 1802.0 55 1617.5 56 1433.0 57 1220.0 58 1044.5 59 917.5 60 785.0 61 665.0 62 542.0 63 416.5 64 299.5 65 225.5 66 193.0 67 158.0 68 115.5 69 91.0 70 78.0 71 62.0 72 42.5 73 35.0 74 34.5 75 34.5 76 28.0 77 17.0 78 12.5 79 9.0 80 9.5 81 7.0 82 3.5 83 4.5 84 4.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22469122187913543 2 0.0 3 0.0 4 0.0 5 0.03032642258491398 6 0.006892368769298633 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002756947507719453 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.892368769298632E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006892368769298633 36-37 0.017920158800176444 38-39 0.013095500661667401 40-41 0.006892368769298633 42-43 0.005513895015438906 44-45 0.0020677106307895898 46-47 6.892368769298632E-4 48-49 0.0013784737538597264 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.892368769298632E-4 58-59 0.0 60-61 0.0013784737538597264 62-63 0.0 64-65 0.0013784737538597264 66-67 0.009649316277018085 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.06272055580061756 78-79 0.15232134980149978 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.09511468901632113 88-89 0.3466861490957212 90-91 0.37149867666519626 92-93 6.892368769298632E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 72544.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.68570798411999 #Duplication Level Percentage of deduplicated Percentage of total 1 91.32152085640458 68.20412439347155 2 5.93576965669989 8.86634318482576 3 1.3159837578442228 2.948555359505955 4 0.533407161314138 1.5935156594618436 5 0.26947212993724623 1.0062858403176005 6 0.1568844592100406 0.7030216144684606 7 0.09597637504614248 0.5017644464049404 8 0.05721668512366187 0.34186149095721213 9 0.04245108896271687 0.28534406704896337 >10 0.21779254337393872 2.9416629907366563 >50 0.02399409376153562 1.1579179532421702 >100 0.018456995201181246 3.236656374062638 >500 0.007382798080472499 3.7908028231142477 >1k 0.0036913990402362494 4.422143802382003 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2064 2.845169827966475 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1144 1.5769739744155271 RNA PCR Primer, Index 17 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 814 1.1220776356418174 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 766 1.0559108954565506 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 628 0.8656815174239083 RNA PCR Primer, Index 17 (95% over 23bp) TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 542 0.7471327745919717 RNA PCR Primer, Index 17 (96% over 28bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.6561535068372298 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 378 0.5210630789589766 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 266 0.36667401852668724 RNA PCR Primer, Index 17 (96% over 26bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 220 0.3032642258491398 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 213 0.2936149095721217 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 202 0.27845169827966476 TruSeq Adapter, Index 12 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.25226069695632997 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.21228495809439787 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 148 0.20401411557123952 No Hit CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA 108 0.14887516541685047 RNA PCR Primer, Index 17 (96% over 28bp) CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 87 0.1199272165857962 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 87 0.1199272165857962 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA 76 0.10476400529333921 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0041354212615791796 0.0 22-23 0.0 0.0 0.0 0.019987869430966032 0.0 24-25 0.0 0.0 0.0 0.03584031760035289 0.0 26-27 0.0 0.0 0.0 0.06616674018526687 0.0 28-29 0.0 0.0 0.0 0.1419827966475518 0.0 30-31 0.0 0.0 0.0 0.2667346713718571 0.0 32-33 0.0 0.0 0.0 0.48177657697397447 0.0 34-35 0.0 0.0 0.0 0.8153672254080282 0.0 36-37 0.0 0.0 0.0 1.279912880458756 0.0 38-39 0.0 0.0 0.0 1.785812748125276 0.0 40-41 0.0 0.0 0.0 2.208314953683282 0.0 42-43 0.0 0.0 0.0 2.6218570798411998 0.0 44-45 0.0 0.0 0.0 3.121553815615351 0.0 46-47 0.0 0.0 0.0 3.643995368328187 0.0 48-49 0.0 0.0 0.0 4.174018526687252 0.0 50-51 0.0 0.0 0.0 4.644767313630348 0.0 52-53 0.0 0.0 0.0 5.118962284958094 0.0 54-55 0.0 0.0 0.0 5.650363917071019 0.0 56-57 0.0 0.0 0.0 6.24862152624614 0.0 58-59 0.0 0.0 0.0 6.899261138067931 0.0 60-61 0.0 0.0 0.0 7.533359064843405 0.0 62-63 0.0 0.0 0.0 8.148847595941774 0.0 64-65 0.0 0.0 0.0 8.796730260255845 0.0 66-67 0.0 0.0 0.0 9.559026246140274 0.0 68-69 0.0 0.0 0.0 10.359919497132775 0.0 70-71 0.0 0.0 0.0 11.148406484340537 0.0 72-73 0.0 0.0 0.0 11.980315394794882 0.0 74-75 0.0 0.0 0.0 12.821184384649317 0.0 76-77 0.0 0.0 0.0 13.71994927216586 0.0 78-79 0.0 0.0 0.0 14.664203793559771 0.0 80-81 0.0 0.0 0.0 15.650501764446405 0.0 82-83 0.0 0.0 0.0 16.57683612704014 0.0 84-85 0.0 0.0 0.0 17.53901080723423 0.0 86-87 0.0 0.0 0.0 18.60663872959859 0.0 88-89 0.0 0.0 0.0 19.699768416409352 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 20 0.002073438 71.187416 3 GTACCCA 25 0.004978776 57.068005 1 TGCGGGG 25 0.0015693532 38.20375 90-91 CCTGGAC 55 0.0025748552 34.51511 3 GGTATCA 760 0.0 34.416008 1 CTTGCAA 30 0.003956659 31.63885 56-57 GAGTACC 40 4.4975986E-4 29.661423 12-13 GTATCAA 940 0.0 27.768139 2 CTAACAT 35 0.008403777 27.119015 94-95 CATGGGG 155 1.3910503E-6 24.494593 4 TATCAAC 1070 0.0 24.394442 3 ATCAACG 1105 0.0 24.051252 4 AACGCAG 1095 0.0 23.83749 7 TCAACGC 1125 0.0 23.623674 5 CAACGCA 1150 0.0 23.110119 6 TACATGG 495 0.0 23.010073 2 GTACATG 500 0.0 22.827202 1 ACATGGG 485 0.0 21.527464 3 GAGTACT 645 0.0 20.602043 12-13 CGCAGAG 1265 0.0 20.25887 9 >>END_MODULE