FastQCFastQC Report
Thu 26 May 2016
SRR938847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118028
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.7659199511980208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.7489748195343477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.4693801470837428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.439726166672315No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.32111024502660385No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.18554919171721965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.14064459280848612No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1420.1203104348120785No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1290.10929609923069103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTCA250.001598448638.071168-69
ACTTGAC500.001635711237.8773548
TGTAGGA600.00399950431.5644632
TCCAGTA753.1204507E-431.564462
GGTATCA11500.030.0548571
CGCCGTA350.00830215527.19364290-91
AGACTTG700.00849448827.0552526
GTACAGG700.00849448827.0552521
CCAGTAC700.00849448827.0552523
ACCCATG700.00849448827.0552523
GTAAACA700.00849448827.0552524
GAGTACT7800.024.58385812-13
GTATCAA14950.022.802422
GTACTTT8400.022.54604314-15
TATCAAC16250.020.9782263
ATCAACG16600.020.8211364
TCAACGC16800.020.5732655
CAACGCA17350.019.9210876
AACGCAG17350.019.9210877
AGTACTT7850.019.90370412-13
Produced by FastQC (version 0.11.5)