FastQCFastQC Report
Thu 26 May 2016
SRR938847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118028
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT18631.5784390144711427No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT9520.8065882671908361TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.7769342867794082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.7260988917883892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.4888670484969668No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG5210.44142067983868233TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT4890.4143084691768055RNA PCR Primer, Index 11 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.36770935710170466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.34059714643982786No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.21689768529501474No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT2370.20079981021452536TruSeq Adapter, Index 11 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.17199308638628122No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1690.14318636255803707No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1460.1236994611448131No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1420.1203104348120785No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA1240.10505981631477275RNA PCR Primer, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1240.10505981631477275TruSeq Adapter, Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG250.00502689956.951959
TACACCA651.3275442E-436.507665
GGGGACC550.002580946834.5163359
CCAGGTT600.003953010331.6399733
CCTGTTC650.00584716829.206138
AGTAGCT700.00839609227.1199743
GGTATCA10850.024.1398741
GTATCAA14500.022.9896351
CTGAGCA1100.002876298721.5727089
AGTACTT8750.020.61118112-13
TATCAAC16400.019.9679092
ATCAACG16450.019.9072173
GAGTACT8650.019.47778512-13
TCAACGC16900.019.3771444
CAACGCA17000.019.2631595
AACGCAG17100.019.150516
TACTTTT9350.019.03474214-15
GTACTTT9100.018.2538314-15
GTGGTGG1350.009215333517.637551
ACGCAGA18850.017.372617
Produced by FastQC (version 0.11.5)