##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938847_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 118028 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811604026163284 31.0 31.0 34.0 30.0 34.0 2 32.03916019927475 33.0 31.0 34.0 30.0 34.0 3 32.08738604398956 33.0 31.0 34.0 30.0 34.0 4 35.78289897312502 37.0 35.0 37.0 35.0 37.0 5 35.684286779408275 37.0 35.0 37.0 33.0 37.0 6 35.720540888602706 37.0 35.0 37.0 33.0 37.0 7 35.63745043548988 37.0 35.0 37.0 33.0 37.0 8 35.635239095807776 37.0 35.0 37.0 33.0 37.0 9 37.21216152099502 39.0 37.0 39.0 33.0 39.0 10-11 37.148943471040766 39.0 37.0 39.0 33.0 39.0 12-13 37.0652006303589 39.0 37.0 39.0 33.0 39.0 14-15 38.197940319246285 40.0 37.0 41.0 33.0 41.0 16-17 38.09808689463517 40.0 37.0 41.0 33.0 41.0 18-19 38.14499101908022 40.0 37.0 41.0 33.0 41.0 20-21 38.2317966923103 40.0 37.5 41.0 33.0 41.0 22-23 38.15545887416545 40.0 37.0 41.0 33.0 41.0 24-25 38.053385637306405 40.0 37.0 41.0 33.0 41.0 26-27 37.79558240417528 40.0 37.0 41.0 32.5 41.0 28-29 37.52612515674247 40.0 36.0 41.0 32.0 41.0 30-31 37.21342393330396 39.0 36.0 41.0 31.5 41.0 32-33 36.86820923848578 39.0 35.0 41.0 31.0 41.0 34-35 36.69819873250415 39.0 35.0 41.0 30.5 41.0 36-37 36.49159521469482 39.0 35.0 41.0 30.0 41.0 38-39 36.188357847290476 39.0 35.0 41.0 30.0 41.0 40-41 35.96659267292507 39.0 35.0 41.0 29.0 41.0 42-43 35.74367099332362 39.0 35.0 41.0 28.5 41.0 44-45 35.377821364422005 39.0 35.0 41.0 26.5 41.0 46-47 34.81528959230013 38.5 34.5 41.0 24.0 41.0 48-49 34.33401819907141 38.0 34.0 40.0 22.0 41.0 50-51 33.91349086657404 38.0 33.5 40.0 18.5 41.0 52-53 33.66203782153387 38.0 33.0 40.0 16.0 41.0 54-55 33.32223285999932 38.0 33.0 40.0 11.0 41.0 56-57 32.92190412444505 37.0 33.0 40.0 5.0 41.0 58-59 32.45191395262141 37.0 32.0 40.0 2.0 41.0 60-61 31.879532822720034 36.5 31.5 40.0 2.0 41.0 62-63 31.29421408479344 36.0 31.0 39.5 2.0 41.0 64-65 30.74762768156709 35.5 30.5 39.0 2.0 40.5 66-67 30.144016674009556 35.0 29.5 39.0 2.0 40.0 68-69 29.592829667536517 35.0 29.0 38.0 2.0 40.0 70-71 29.111210899108684 35.0 28.0 37.5 2.0 40.0 72-73 28.76550903175518 34.5 28.0 37.0 2.0 39.0 74-75 28.352420612058154 34.0 27.0 36.5 2.0 39.0 76-77 26.449393364286443 32.0 24.5 34.5 2.0 36.5 78-79 27.38019791913783 33.5 26.0 35.0 2.0 37.0 80-81 27.405149625512593 34.0 26.0 35.0 2.0 37.0 82-83 27.100840478530518 34.0 26.0 35.0 2.0 36.5 84-85 26.751800420239267 34.0 25.0 35.0 2.0 36.0 86-87 26.235156064662625 33.0 23.5 35.0 2.0 36.0 88-89 25.71071694852069 33.0 20.5 35.0 2.0 35.0 90-91 25.33966092791541 33.0 18.5 35.0 2.0 35.0 92-93 25.074961026197172 33.0 17.5 35.0 2.0 35.0 94-95 24.72205324160369 32.5 9.5 35.0 2.0 35.0 96-97 24.226941912088655 32.0 2.0 35.0 2.0 35.0 98-99 23.572999627207103 32.0 2.0 34.0 2.0 35.0 100-101 20.98336411698919 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6249120389373992 1101 2 0.23646759893499336 1101 3 0.33420676178117503 1101 4 0.38037880470675844 1101 5 0.11316433258549097 1101 6 0.2401731989100071 1101 7 0.7748078401092258 1101 8 0.48655536968739455 1101 9 0.6723521620848842 1101 10-11 0.46475135745770757 1101 12-13 0.5340181273597224 1101 14-15 0.7299127633546334 1101 16-17 0.9081602780910174 1101 18-19 0.42189123062289013 1101 20-21 0.385093628180293 1101 22-23 0.6000086953331376 1101 24-25 0.6415857998129795 1101 26-27 0.8542621725492481 1101 28-29 0.9410515051676853 1101 30-31 0.5740725089893672 1101 32-33 1.2470263240772397 1101 34-35 1.4211382744093441 1101 36-37 1.1072930855052547 1101 38-39 0.7082123571418961 1101 40-41 0.6744475446767879 1101 42-43 1.2442293115740526 1101 44-45 1.4301673146254785 1101 46-47 1.3761141071956544 1101 48-49 1.2683497864503934 1101 50-51 1.3982124889428036 1101 52-53 0.743307878043808 1101 54-55 -0.035291837303596196 1101 56-57 -0.7119049192936728 1101 58-59 -0.2073718277552743 1101 60-61 -0.1570168259128586 1101 62-63 -0.483972402733432 1101 64-65 0.11345146053766086 1101 66-67 0.5056401681642413 1101 68-69 0.6128198794614974 1101 70-71 0.061337708012935366 1101 72-73 0.13905465768285907 1101 74-75 0.5159121522919996 1101 76-77 0.5367997691220552 1101 78-79 -0.2706207094433033 1101 80-81 -0.47327467556823777 1101 82-83 -0.7857178244210026 1101 84-85 -0.4173402255833132 1101 86-87 0.23435900692986422 1101 88-89 0.27495945543145695 1101 90-91 0.19015858266542907 1101 92-93 0.8117813535993967 1101 94-95 0.9012185758976337 1101 96-97 0.39975436614568594 1101 98-99 -0.07003507063891945 1101 100-101 1.2894630245325658 1102 1 0.6165902636253371 1102 2 0.5263427723053127 1102 3 0.495527843611967 1102 4 0.402830261017435 1102 5 0.33334463771725353 1102 6 0.16059622248837258 1102 7 0.22106304121879816 1102 8 0.375858421005006 1102 9 0.3847308028615828 1102 10-11 0.26593894414287433 1102 12-13 0.25640890405875183 1102 14-15 0.27399216696073836 1102 16-17 0.6533059091589806 1102 18-19 0.4773697327033304 1102 20-21 0.006539536640765675 1102 22-23 -0.19008319093593684 1102 24-25 -0.10074256357121669 1102 26-27 -0.2977835972149663 1102 28-29 -0.1653111855396716 1102 30-31 0.23990121911419493 1102 32-33 0.08509844612994044 1102 34-35 -0.06785271033406559 1102 36-37 -0.23806058994550483 1102 38-39 0.3641156165038879 1102 40-41 0.6349624545242207 1102 42-43 0.1652157886891743 1102 44-45 -0.4936797727531612 1102 46-47 -0.37882694689587737 1102 48-49 -0.47995985293933785 1102 50-51 -0.9066592170211294 1102 52-53 -0.38515952833621725 1102 54-55 -0.028053626485295524 1102 56-57 -0.015779745923353516 1102 58-59 0.3703414177371016 1102 60-61 -0.5886222350668113 1102 62-63 -0.666357978322889 1102 64-65 -0.39387343544014897 1102 66-67 -0.5714662812116273 1102 68-69 0.03957091968341331 1102 70-71 0.21598403256217225 1102 72-73 -0.10587426048634896 1102 74-75 0.19296659057216559 1102 76-77 -0.14276853878363127 1102 78-79 -0.07445219349323295 1102 80-81 -0.09225005698294098 1102 82-83 -0.026529197513927016 1102 84-85 -0.09101394264294882 1102 86-87 -0.5022481359272781 1102 88-89 -0.18390843638546173 1102 90-91 -0.18913233272985508 1102 92-93 -0.26055741200393 1102 94-95 0.6430268283248175 1102 96-97 0.05947177252571123 1102 98-99 0.746149048639861 1102 100-101 1.1459124003994177 1103 1 -0.03216465118462608 1103 2 -0.06343442297646718 1103 3 -0.4054387356802991 1103 4 0.1694587013844071 1103 5 0.1508413611772781 1103 6 -0.2730538430424261 1103 7 -0.36904180806036635 1103 8 -0.08162847283509933 1103 9 -0.09348020631272647 1103 10-11 -0.27235561680078035 1103 12-13 -0.24330439869354592 1103 14-15 -0.11603896016901416 1103 16-17 -0.22513445781349617 1103 18-19 -0.1471387863005944 1103 20-21 0.018744648043124812 1103 22-23 0.2728509506105894 1103 24-25 -0.19719570904958061 1103 26-27 -0.6229801896134006 1103 28-29 -0.2740103991700735 1103 30-31 -0.4667665397324683 1103 32-33 -0.22781112136678416 1103 34-35 -0.5636751218674831 1103 36-37 0.01292237204663138 1103 38-39 0.2783112259403282 1103 40-41 -0.1492364314521879 1103 42-43 -0.1524038050198655 1103 44-45 -0.12470041495499373 1103 46-47 -0.012591691325766874 1103 48-49 0.31996642491125016 1103 50-51 0.4753034304231676 1103 52-53 0.5360396852941847 1103 54-55 -0.09608278769105993 1103 56-57 0.23589948081321666 1103 58-59 0.19754489086947302 1103 60-61 0.4586418540550774 1103 62-63 0.6173903834071233 1103 64-65 -0.2463062663706843 1103 66-67 0.34731648418810934 1103 68-69 0.13909582138721177 1103 70-71 0.09082020556348525 1103 72-73 -0.123526619595129 1103 74-75 0.24575984089981873 1103 76-77 0.8312239251744273 1103 78-79 0.6053479375683644 1103 80-81 -0.11899481451898097 1103 82-83 0.39794655609026464 1103 84-85 0.7679719682351624 1103 86-87 0.7481204748197712 1103 88-89 1.1578756396302659 1103 90-91 1.3661183225781421 1103 92-93 0.9120752878650045 1103 94-95 1.1295520576826164 1103 96-97 0.9969308157760395 1103 98-99 1.0028249988364522 1103 100-101 1.3287433309367707 1104 1 0.31066034448786795 1104 2 0.2473670310654228 1104 3 0.32184185978447744 1104 4 0.13985856290423015 1104 5 -0.023124364978166057 1104 6 -0.02370296888629042 1104 7 -0.2472657997515526 1104 8 0.22744197895649165 1104 9 0.04274967078610814 1104 10-11 0.24850296031537766 1104 12-13 0.054168338021014506 1104 14-15 0.2218526790901123 1104 16-17 0.016513456328787868 1104 18-19 0.3062107030537433 1104 20-21 -0.09100089462607741 1104 22-23 0.12991343979183512 1104 24-25 -0.0019386552154116998 1104 26-27 -0.04205583441173388 1104 28-29 -0.002069945647484417 1104 30-31 -0.685132473608185 1104 32-33 -0.31786651343879413 1104 34-35 -0.4649214434877038 1104 36-37 -0.6086805360371486 1104 38-39 -0.31235001691928943 1104 40-41 0.05047795317651804 1104 42-43 0.22426228464605202 1104 44-45 0.28874947252985095 1104 46-47 -0.257701609968656 1104 48-49 -0.4386020362277492 1104 50-51 -1.0928115081262604 1104 52-53 -1.1604290701151996 1104 54-55 -0.9556985051914921 1104 56-57 -1.0311941664085253 1104 58-59 -0.6972967224246283 1104 60-61 -0.5527394857406627 1104 62-63 -0.4931773853309771 1104 64-65 -0.5290654839576696 1104 66-67 0.12470959479915322 1104 68-69 -0.12367032020877389 1104 70-71 0.02460936948939718 1104 72-73 -0.6374611877100698 1104 74-75 0.43833384124601693 1104 76-77 0.4405367172810557 1104 78-79 0.6821178873692979 1104 80-81 0.8904824228014263 1104 82-83 0.48324169197394085 1104 84-85 0.651994817464864 1104 86-87 1.2720948559864667 1104 88-89 1.169949380326127 1104 90-91 0.9763831659224387 1104 92-93 0.9765551216887935 1104 94-95 0.6907862622870802 1104 96-97 1.345944144493366 1104 98-99 1.2457278896102117 1104 100-101 1.5124892198064295 1105 1 0.3051359521710246 1105 2 -0.11715787119532806 1105 3 0.005421666005787529 1105 4 0.07118611687329235 1105 5 -0.08074802637541012 1105 6 0.21384590073805043 1105 7 0.05793562809137853 1105 8 0.38526961791620806 1105 9 0.09306413619491849 1105 10-11 0.20235303680696148 1105 12-13 0.02977602117586997 1105 14-15 0.18836636258493655 1105 16-17 -0.1642616584085843 1105 18-19 0.2804585357921354 1105 20-21 -0.18916509656386893 1105 22-23 0.053039395936650635 1105 24-25 0.0658838843052365 1105 26-27 0.18886376443239072 1105 28-29 0.11292240519392038 1105 30-31 0.19027123198420526 1105 32-33 0.0847766958081877 1105 34-35 -0.6655843705657247 1105 36-37 -0.36611721800213815 1105 38-39 0.06726072589899701 1105 40-41 -0.05265820184643388 1105 42-43 0.3810700357934209 1105 44-45 0.20817833535495112 1105 46-47 0.3731839926150542 1105 48-49 0.38397997975049236 1105 50-51 -0.059410182272095824 1105 52-53 0.018476250299556796 1105 54-55 -0.04281392249558991 1105 56-57 -0.07904390293751362 1105 58-59 -0.07660999796431867 1105 60-61 -0.1121904461350205 1105 62-63 -0.03894485090976474 1105 64-65 0.04181671900000694 1105 66-67 0.92897612548078 1105 68-69 1.4144904821029733 1105 70-71 1.5473868639650057 1105 72-73 1.373332624970537 1105 74-75 1.1216586755142508 1105 76-77 1.4913611265960327 1105 78-79 1.5729898914488487 1105 80-81 1.3974941515112675 1105 82-83 2.0058067098219787 1105 84-85 1.7757412053621984 1105 86-87 1.405530178101035 1105 88-89 1.3754142791872503 1105 90-91 1.6502740379265184 1105 92-93 1.2328869251904067 1105 94-95 1.7947723238203146 1105 96-97 1.9263877956917383 1105 98-99 2.4157516869925004 1105 100-101 2.399451653938673 1106 1 0.045161692196764136 1106 2 -0.229959748703088 1106 3 -0.02705619000147408 1106 4 0.08087647950483046 1106 5 -0.013294017744932773 1106 6 0.3003021048413146 1106 7 0.1685420328154308 1106 8 -0.038532335297510656 1106 9 0.17989887008847205 1106 10-11 -0.06678164409242271 1106 12-13 0.4546757107814372 1106 14-15 0.1692127551960354 1106 16-17 0.06717145537746916 1106 18-19 0.23787393438400528 1106 20-21 0.29277903243908554 1106 22-23 0.2854945401565061 1106 24-25 0.7608645792859292 1106 26-27 -0.021523093013286143 1106 28-29 0.20706276404016677 1106 30-31 0.6479894544083109 1106 32-33 0.43173710159212675 1106 34-35 0.6585128358844159 1106 36-37 0.8375696621553388 1106 38-39 0.6175294820501094 1106 40-41 0.7087544713309484 1106 42-43 1.419680074403452 1106 44-45 0.8311626642216297 1106 46-47 0.9504797757931911 1106 48-49 1.3454077941194882 1106 50-51 1.6957567493654224 1106 52-53 1.5843782867898284 1106 54-55 1.1649610793970595 1106 56-57 1.3468778171018556 1106 58-59 1.145814106812729 1106 60-61 1.250138269134876 1106 62-63 1.6781798368031637 1106 64-65 1.0638471527951445 1106 66-67 1.9088383406371143 1106 68-69 2.2722389869103026 1106 70-71 2.0058999989487276 1106 72-73 2.3030017899338198 1106 74-75 2.7341955821688018 1106 76-77 2.7209394444096446 1106 78-79 2.866199067010964 1106 80-81 2.6637898589834457 1106 82-83 2.623680886519683 1106 84-85 2.3588284943318385 1106 86-87 2.4906615279382684 1106 88-89 2.3195054291607526 1106 90-91 3.296424389959224 1106 92-93 3.135188386315395 1106 94-95 2.6480163241231374 1106 96-97 2.2687679910131067 1106 98-99 2.5631133343541457 1106 100-101 3.7299460138447955 1107 1 -0.22864783161276137 1107 2 -0.09508579912325743 1107 3 -0.06935310876898626 1107 4 0.10699692768410074 1107 5 -0.1107029813303626 1107 6 0.008215270107420736 1107 7 -0.15512743497168202 1107 8 0.10621556386215047 1107 9 -0.02360253047174865 1107 10-11 0.02814132509524825 1107 12-13 0.008492237392083268 1107 14-15 -7.10213991645503E-4 1107 16-17 0.34497257582564345 1107 18-19 0.35951258984619017 1107 20-21 -0.020246177644949626 1107 22-23 -0.23502366712641276 1107 24-25 0.13288838880974652 1107 26-27 -0.11980740673877932 1107 28-29 0.038260243031764674 1107 30-31 -0.29700354279056995 1107 32-33 0.14581273184422372 1107 34-35 0.5220282420468791 1107 36-37 0.3053917910068762 1107 38-39 0.28167514031341767 1107 40-41 0.46085531166708193 1107 42-43 0.2470610267844151 1107 44-45 0.10840356058017164 1107 46-47 0.5604587673898322 1107 48-49 0.41855205182256583 1107 50-51 -0.3929687408306535 1107 52-53 -1.0137309569076507 1107 54-55 -1.1104941026757658 1107 56-57 -1.326791650685255 1107 58-59 -1.3180514394057603 1107 60-61 -1.1308841398287122 1107 62-63 -0.5925484545133664 1107 64-65 -1.1721472449639556 1107 66-67 -1.1997400907354354 1107 68-69 -0.4890005088880187 1107 70-71 -0.5822302531521117 1107 72-73 -0.8311718795339651 1107 74-75 -0.6960215046659322 1107 76-77 -0.6603280625931554 1107 78-79 -0.348856955397995 1107 80-81 -0.4788571998400819 1107 82-83 -0.19914824513297802 1107 84-85 -0.017502037881047983 1107 86-87 0.2301923402631978 1107 88-89 0.38127013504310625 1107 90-91 0.19926052441300257 1107 92-93 0.32545449275797367 1107 94-95 0.05344349499148393 1107 96-97 0.5234598677638083 1107 98-99 0.24525619149700262 1107 100-101 0.25722192420894174 1108 1 -0.32983830780323586 1108 2 -0.16115722769468377 1108 3 -0.1651864421023177 1108 4 -0.4007411675539956 1108 5 -0.13094107656846177 1108 6 -0.16976092036876622 1108 7 0.14963446979022166 1108 8 -0.1152130075664175 1108 9 0.6258022314330134 1108 10-11 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0.05335802053118499 2316 84-85 -0.620384243394831 2316 86-87 -0.42729512840848116 2316 88-89 -0.7386546769869682 2316 90-91 -0.6076003778426369 2316 92-93 -1.1343825999738577 2316 94-95 -1.392452494983452 2316 96-97 -1.5779812349930822 2316 98-99 -1.6248565904578811 2316 100-101 -1.621004892833664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 9.0 6 26.0 7 71.0 8 122.0 9 180.0 10 336.0 11 485.0 12 694.0 13 913.0 14 1100.0 15 1381.0 16 1765.0 17 2176.0 18 2093.0 19 1508.0 20 1484.0 21 1522.0 22 1568.0 23 1581.0 24 1810.0 25 1875.0 26 1773.0 27 1823.0 28 2165.0 29 2507.0 30 2985.0 31 3750.0 32 4312.0 33 5365.0 34 7000.0 35 9573.0 36 13766.0 37 20738.0 38 17772.0 39 1798.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.304599607132836 21.320271775640535 16.190038861535584 22.185089755691045 2 15.812349611956487 22.390449723794354 39.24577218964991 22.55142847459925 3 18.706578100111837 26.576744501304777 33.49967804249839 21.216999356084997 4 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31.068475277052904 20.75100823533399 24-25 20.750160978750806 28.213220591723996 31.59504524350154 19.441573186023657 26-27 20.87258955502084 27.770952655302132 31.779323550343985 19.577134239333038 28-29 18.815874199342527 26.878791473209745 33.16331717897448 21.142017148473244 30-31 20.181651811434577 27.23506286643847 33.045972142203546 19.53731317992341 32-33 19.40556478123835 28.551699596705866 31.58403090792015 20.458704714135628 34-35 19.39128703307504 28.17542863679307 32.71832197221646 19.71496235791543 36-37 20.80995881565344 27.48546684066912 31.55390403877769 20.15067030489975 38-39 20.2946198201099 26.3094567285083 32.52807870085962 20.86784475052217 40-41 19.65294164996759 26.630119343673346 33.219228862782316 20.497710143576754 42-43 20.25630163101038 27.226435077314125 32.30713831815294 20.210124973522557 44-45 19.33930091970871 27.91110470953557 31.841577950714456 20.90801642004126 46-47 20.698493122249005 26.820898873548515 30.06002940042618 22.420578603776303 48-49 20.2892533974989 27.05163181617921 30.421171247500595 22.2379435388213 50-51 19.92281492527197 27.588368861626055 30.86598095367201 21.622835259429966 52-53 19.603399193411732 28.05647812383502 31.540397193886193 20.79972548886705 54-55 19.251787711390516 28.139085640695427 30.493611685362794 22.11551496255126 56-57 20.128698274985595 28.799098518995493 28.91178364455892 22.160419561459992 58-59 18.986676833788735 29.21522526529834 30.27430048082015 21.523797420092773 60-61 19.672533943360655 29.71934506788672 28.93054584736608 21.67757514138654 62-63 19.325499034127493 30.17843223641848 28.68302436709933 21.813044362354695 64-65 19.163249398447828 30.288999220523944 28.964737181007898 21.583014200020333 66-67 18.943283683256233 29.804026231508313 28.89659905444563 22.356091030789827 68-69 18.765541928787783 29.65961322573129 28.79456058969308 22.780284255787848 70-71 19.05054416206667 29.042876327573282 28.21000546492072 23.696574045439334 72-73 19.068356661131258 28.519080218253297 28.396651641983254 24.01591147863219 74-75 19.11326126003999 28.6224455214017 28.397922526858032 23.866370691700276 76-77 19.39230244105139 28.307630988674575 28.528542535012996 23.771524035261045 78-79 19.019850485667305 27.963587665915142 28.820074248614198 24.19648759980336 80-81 18.893821804995426 28.21364422001559 28.424187480936723 24.46834649405226 82-83 18.887043752329955 27.7934049547565 28.430965533602198 24.88858575931135 84-85 18.898058087911345 27.17575490561562 28.530518182126276 25.395668824346767 86-87 19.207941794115776 27.031796873342806 28.472944021381753 25.28731731115966 88-89 19.302779277192418 26.793524228887648 28.268806555676925 25.634889938243006 90-91 18.78602527344165 26.583837740256982 28.762875650419456 25.867261335881913 92-93 18.693445623072492 26.729250686277833 28.54153251770766 26.035771172942013 94-95 18.474006168027927 26.638594231877182 28.567797471786356 26.31960212830854 96-97 18.598976514047514 26.2645304504016 28.3530179279493 26.783475107601586 98-99 18.61049331723539 26.294295820889204 28.168435322276586 26.926775539598825 100-101 18.71224380862006 26.08555669465461 28.801037050844297 26.401162445881027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 418.0 1 277.0 2 142.5 3 135.0 4 111.0 5 108.5 6 174.5 7 270.0 8 342.5 9 396.5 10 365.5 11 309.0 12 309.0 13 287.0 14 277.0 15 318.5 16 360.5 17 370.5 18 372.0 19 408.0 20 447.0 21 510.5 22 585.5 23 656.0 24 747.0 25 835.0 26 957.0 27 1166.0 28 1334.5 29 1440.5 30 1623.5 31 1874.0 32 2028.0 33 2198.5 34 2432.0 35 2602.5 36 2816.5 37 3016.0 38 3050.0 39 3043.5 40 3178.5 41 3384.0 42 3634.5 43 3857.5 44 4101.5 45 4434.0 46 4676.5 47 4812.0 48 4997.5 49 4930.0 50 4624.5 51 4313.5 52 3864.0 53 3489.5 54 3204.5 55 2900.0 56 2604.5 57 2267.0 58 1985.0 59 1726.5 60 1418.0 61 1143.0 62 904.5 63 672.5 64 488.0 65 356.0 66 265.0 67 212.5 68 151.5 69 116.0 70 92.0 71 68.5 72 52.5 73 48.5 74 44.0 75 27.5 76 17.5 77 15.0 78 15.5 79 10.5 80 5.5 81 4.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3651675873521537 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0021181414579591286 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0072016809570610366 36-37 0.018639644830040332 38-39 0.00974345070661199 40-41 0.006354424373877385 42-43 0.002541769749550954 44-45 0.001270884874775477 46-47 0.0021181414579591286 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.2362829159182567E-4 60-61 4.2362829159182567E-4 62-63 0.0 64-65 0.0 66-67 0.0016945131663673027 68-69 4.2362829159182567E-4 70-71 0.0021181414579591286 72-73 0.0 74-75 0.0 76-77 0.09108008269224252 78-79 0.03897380282644796 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14445724743281255 88-89 0.3300064391500322 90-91 0.267309451994442 92-93 0.0 94-95 0.0 96-97 0.0 98-99 4.2362829159182567E-4 100-101 8.472565831836513E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 118028.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.53668621005185 #Duplication Level Percentage of deduplicated Percentage of total 1 90.55018303010436 70.20961127867965 2 6.59564005900672 10.228081472193038 3 1.3298366388023821 3.093333785203511 4 0.5572856908703491 1.7284034296946489 5 0.2939408840080861 1.1395601043820112 6 0.17483472654756052 0.8133663198563053 7 0.10817898705130306 0.5871488121462703 8 0.07321204174179097 0.4541295285864371 9 0.049172266841501396 0.3431389161893788 >10 0.23274872971643995 3.2949808520012196 >50 0.014205321531989291 0.8023519842749177 >100 0.014205321531989291 2.4875453282272004 >500 0.005463585204611266 3.2399091740942825 >1k 0.0010927170409222532 1.5784390144711427 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1863 1.5784390144711427 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 952 0.8065882671908361 TruSeq Adapter, Index 11 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 917 0.7769342867794082 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 857 0.7260988917883892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 577 0.4888670484969668 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 521 0.44142067983868233 TruSeq Adapter, Index 11 (96% over 28bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 489 0.4143084691768055 RNA PCR Primer, Index 11 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 434 0.36770935710170466 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.34059714643982786 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.21689768529501474 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 237 0.20079981021452536 TruSeq Adapter, Index 11 (96% over 26bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 0.17199308638628122 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 169 0.14318636255803707 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 146 0.1236994611448131 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 142 0.1203104348120785 No Hit CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA 124 0.10505981631477275 RNA PCR Primer, Index 11 (96% over 28bp) CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 124 0.10505981631477275 TruSeq Adapter, Index 11 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0021181414579591286 0.0 22-23 0.0 0.0 0.0 0.011437963872979292 0.0 24-25 0.0 0.0 0.0 0.022875927745958584 0.0 26-27 0.0 0.0 0.0 0.041939200867590745 0.0 28-29 0.0 0.0 0.0 0.10209441827362999 0.0 30-31 0.0 0.0 0.0 0.20545972142203545 0.0 32-33 0.0 0.0 0.0 0.38889077168129593 0.0 34-35 0.0 0.0 0.0 0.6816179211712474 0.0 36-37 0.0 0.0 0.0 1.0997390449723794 0.0 38-39 0.0 0.0 0.0 1.5195546819398786 0.0 40-41 0.0 0.0 0.0 1.910139966787542 0.0 42-43 0.0 0.0 0.0 2.310892330633409 0.0 44-45 0.0 0.0 0.0 2.8128918561697223 0.0 46-47 0.0 0.0 0.0 3.375470227403667 0.0 48-49 0.0 0.0 0.0 3.852052055444471 0.0 50-51 0.0 0.0 0.0 4.334141051275969 0.0 52-53 0.0 0.0 0.0 4.814111905649507 0.0 54-55 0.0 0.0 0.0 5.337716474057004 0.0 56-57 0.0 0.0 0.0 6.008320059646863 0.0 58-59 0.0 0.0 0.0 6.66409665503101 0.0 60-61 0.0 0.0 0.0 7.338089266953604 0.0 62-63 0.0 0.0 0.0 8.006151082793913 0.0 64-65 0.0 0.0 0.0 8.73182634629071 0.0 66-67 0.0 0.0 0.0 9.53248381739926 0.0 68-69 0.0 0.0 0.0 10.386094824956789 0.0 70-71 0.0 0.0 0.0 11.278256007049174 0.0 72-73 0.0 0.0 0.0 12.140339580438539 0.0 74-75 0.0 0.0 0.0 13.064272884400312 0.0 76-77 0.0 0.0 0.0 13.957281323075879 0.0 78-79 0.0 0.0 0.0 14.953655064899854 0.0 80-81 0.0 0.0 0.0 16.024163757752397 0.0 82-83 0.0 0.0 0.0 17.10356864472837 0.0 84-85 0.0 0.0 0.0 18.255837597858136 0.0 86-87 0.0 0.0 0.0 19.447503982105943 0.0 88-89 0.0 0.0 0.0 20.560375504117665 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGTCG 25 0.005026899 56.95195 9 TACACCA 65 1.3275442E-4 36.50766 5 GGGGACC 55 0.0025809468 34.516335 9 CCAGGTT 60 0.0039530103 31.639973 3 CCTGTTC 65 0.005847168 29.20613 8 AGTAGCT 70 0.008396092 27.119974 3 GGTATCA 1085 0.0 24.139874 1 GTATCAA 1450 0.0 22.989635 1 CTGAGCA 110 0.0028762987 21.572708 9 AGTACTT 875 0.0 20.611181 12-13 TATCAAC 1640 0.0 19.967909 2 ATCAACG 1645 0.0 19.907217 3 GAGTACT 865 0.0 19.477785 12-13 TCAACGC 1690 0.0 19.377144 4 CAACGCA 1700 0.0 19.263159 5 AACGCAG 1710 0.0 19.15051 6 TACTTTT 935 0.0 19.034742 14-15 GTACTTT 910 0.0 18.25383 14-15 GTGGTGG 135 0.0092153335 17.63755 1 ACGCAGA 1885 0.0 17.37261 7 >>END_MODULE