FastQCFastQC Report
Thu 26 May 2016
SRR938846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117161
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.7980471317247207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.7570778672083713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.4583436467766578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.41737438226030843No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.3379964322598817No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.21082100699038075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.18436169032357183No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1460.12461484623722911No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1270.10839784569950753No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.10839784569950753No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCT200.00208063871.152844
GTACCCA407.4688232E-659.421061
TACCCAT451.5197593E-552.705812
TACAAGA405.4044655E-447.4352262
AGGATAT250.001603663738.0457832-33
GTAGCCC550.00258745834.498353
GAGTACC552.7411334E-630.19896512-13
AGAGGGC804.50513E-429.6470178
GGTATCA11700.029.2534451
CAACAGT650.005861868629.1909084
GTCCCAG856.343651E-427.9628491
ACCCATG700.0084171627.1058463
GAGTACT7900.023.12705612-13
GTATCAA14700.022.9108922
GTACTTT8700.021.27316314-15
CAACGCA16900.021.0511366
AACGCAG16950.020.989047
ATCAACG16900.020.7704544
TCAACGC17000.020.6482755
AGTACTT8100.020.5054712-13