FastQCFastQC Report
Thu 26 May 2016
SRR938846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117161
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT18261.5585391043094545No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT9480.8091429741978986TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.7852442365633615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.7024521811865723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.5027270166693695No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG5490.46858596290574506TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT4930.42078848763667087RNA PCR Primer, Index 11 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.40115738172258686No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.3140976946253446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.22106332311946805No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.18606874301175305No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT2000.17070526881812206TruSeq Adapter, Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1750.1493671102158568No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1400.11949368817268545No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1370.11693310914041362No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1360.11607958279632302TruSeq Adapter, Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACC250.00503714156.922416
ATGTGTA650.005861841629.1909794
CTGTGTA650.005861841629.1909799
GTGTAGA700.00834720727.1522621
GGCCAAC350.00841648527.11748166-67
CTTTACA908.881396E-426.3980331
ACAGTCA950.001229715424.965978
GAGTACT8400.022.30590412-13
GGTATCA12800.021.5308971
CATGGGG2657.8398443E-1021.4801524
ATGGGGA1353.802761E-421.0823755
GTATCAA16600.020.3233051
AGTACTT8650.020.2902612-13
ACATGGG5800.019.6284163
GTACTTT9500.019.22379514-15
GTACATG6250.019.0065841
TATCAAC17750.018.9741362
ATCAACG18000.018.7106063
TCAACGC18400.018.5616554
GACACAT650.00758642718.25215120-21
Produced by FastQC (version 0.11.5)