##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938846_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117161 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861856761208934 31.0 31.0 34.0 30.0 34.0 2 32.06655798431218 33.0 31.0 34.0 30.0 34.0 3 32.11412500746835 34.0 31.0 34.0 30.0 34.0 4 35.79958347914408 37.0 35.0 37.0 35.0 37.0 5 35.69314874403599 37.0 35.0 37.0 33.0 37.0 6 35.72384155136948 37.0 35.0 37.0 33.0 37.0 7 35.6249434538797 37.0 35.0 37.0 33.0 37.0 8 35.636525806369015 37.0 35.0 37.0 33.0 37.0 9 37.22228386579152 39.0 37.0 39.0 33.0 39.0 10-11 37.169237203506285 39.0 37.0 39.0 33.0 39.0 12-13 37.0766594685945 39.0 37.0 39.0 33.0 39.0 14-15 38.185338977987556 40.0 37.0 41.0 33.0 41.0 16-17 38.09419516733384 40.0 37.0 41.0 33.0 41.0 18-19 38.24560220551207 40.0 37.5 41.0 33.0 41.0 20-21 38.26217768711432 40.0 37.5 41.0 33.0 41.0 22-23 38.16086411007076 40.0 37.0 41.0 33.0 41.0 24-25 38.057109447683104 40.0 37.0 41.0 33.0 41.0 26-27 37.761981376055175 40.0 37.0 41.0 32.5 41.0 28-29 37.49381620163706 40.0 36.0 41.0 32.0 41.0 30-31 37.16783742030198 39.0 36.0 41.0 31.0 41.0 32-33 36.88136410580313 39.0 35.0 41.0 31.0 41.0 34-35 36.723474535041525 39.0 35.0 41.0 30.5 41.0 36-37 36.46372939800787 39.0 35.0 41.0 30.0 41.0 38-39 36.15004139602769 39.0 35.0 41.0 30.0 41.0 40-41 35.925491417792614 39.0 35.0 41.0 29.0 41.0 42-43 35.64459163032067 39.0 35.0 41.0 27.5 41.0 44-45 35.264170671127765 39.0 35.0 41.0 26.5 41.0 46-47 34.69000776708973 38.0 34.5 40.5 23.5 41.0 48-49 34.18786968359778 38.0 34.0 40.0 21.0 41.0 50-51 33.738001553417945 38.0 33.0 40.0 16.5 41.0 52-53 33.46106212818259 38.0 33.0 40.0 13.5 41.0 54-55 33.0984030522102 37.5 33.0 40.0 8.5 41.0 56-57 32.67754628246601 37.0 32.5 40.0 3.5 41.0 58-59 32.19098505475372 37.0 32.0 40.0 2.0 41.0 60-61 31.59417382917524 36.0 31.0 40.0 2.0 41.0 62-63 31.04096499688463 36.0 30.5 39.0 2.0 41.0 64-65 30.459227046542793 35.0 29.5 39.0 2.0 40.0 66-67 29.8447606285368 35.0 29.0 38.5 2.0 40.0 68-69 29.41455347769309 35.0 28.5 38.0 2.0 40.0 70-71 28.98285692337894 35.0 28.0 37.5 2.0 40.0 72-73 28.52608803270713 34.0 27.0 37.0 2.0 39.0 74-75 28.05531277472879 34.0 26.0 36.0 2.0 39.0 76-77 26.199844658205375 31.5 23.5 34.5 2.0 36.5 78-79 27.03042394653511 33.5 25.0 35.0 2.0 37.0 80-81 27.065141130580997 34.0 25.0 35.0 2.0 37.0 82-83 26.76329153899335 34.0 25.0 35.0 2.0 36.5 84-85 26.38573416068487 33.5 23.5 35.0 2.0 36.0 86-87 25.84779064705832 33.0 21.0 35.0 2.0 36.0 88-89 25.245935080786268 33.0 18.5 35.0 2.0 35.0 90-91 24.7435622775497 32.0 12.5 35.0 2.0 35.0 92-93 24.461433412142267 32.0 7.5 35.0 2.0 35.0 94-95 24.147160744616382 32.0 2.0 34.5 2.0 35.0 96-97 23.558440948779882 32.0 2.0 34.0 2.0 35.0 98-99 22.8179897747544 31.0 2.0 34.0 2.0 35.0 100-101 19.774173999880507 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26377759159043634 1101 2 0.14323127175494932 1101 3 0.054425158277872754 1101 4 -0.1517182209709631 1101 5 0.39206547704112893 1101 6 0.19081867778719186 1101 7 0.6205706021061204 1101 8 0.1898970546420955 1101 9 0.39974181414497423 1101 10-11 0.6605982229197593 1101 12-13 0.48139937070583727 1101 14-15 0.44274378826743543 1101 16-17 0.7138570766613839 1101 18-19 0.2417571442900197 1101 20-21 0.5493766080078188 1101 22-23 0.4378436471654936 1101 24-25 0.8711358397102131 1101 26-27 0.5034556352572537 1101 28-29 0.2930305185239277 1101 30-31 0.6732848574238446 1101 32-33 1.120989177271987 1101 34-35 1.4358560382298933 1101 36-37 1.0336633243883213 1101 38-39 1.5546913403704394 1101 40-41 1.189909505566959 1101 42-43 1.38381037810408 1101 44-45 1.4382772600358535 1101 46-47 1.5403660501625183 1101 48-49 1.2529055124490753 1101 50-51 1.1147109114779354 1101 52-53 1.201486811507685 1101 54-55 1.3847054574336184 1101 56-57 1.1486679218183369 1101 58-59 1.3640988341303526 1101 60-61 0.574430951980279 1101 62-63 0.40804957087879856 1101 64-65 0.27121656954011186 1101 66-67 0.33789442747903564 1101 68-69 0.8800029552161419 1101 70-71 1.128696048357984 1101 72-73 1.1739934049625838 1101 74-75 1.3403844932412348 1101 76-77 1.388075707030108 1101 78-79 0.8644498676316807 1101 80-81 1.448998231976102 1101 82-83 1.338636562909663 1101 84-85 1.3060072397682667 1101 86-87 1.7429445547116416 1101 88-89 1.0119637151894274 1101 90-91 0.52651635030373 1101 92-93 0.22832843526161994 1101 94-95 0.8030116596803545 1101 96-97 0.14871467651930814 1101 98-99 0.06861899718866127 1101 100-101 1.01546070338172 1102 1 -0.0805147982627048 1102 2 0.1876237951194355 1102 3 0.4223323678906894 1102 4 0.10562490319459528 1102 5 -0.629963895455532 1102 6 -0.037151949716147215 1102 7 -0.07610830310082406 1102 8 -0.15806689729382128 1102 9 0.2845882760942402 1102 10-11 0.3077527623056113 1102 12-13 0.21633762170717574 1102 14-15 0.5784246961446087 1102 16-17 0.4308964625091747 1102 18-19 0.0892621509655882 1102 20-21 0.5434103196233053 1102 22-23 0.43129324663144786 1102 24-25 0.5330183497235623 1102 26-27 0.20409815862173986 1102 28-29 0.2115885959604995 1102 30-31 0.4738940029512122 1102 32-33 0.2032238234669066 1102 34-35 0.6217705909668751 1102 36-37 0.2506609879397246 1102 38-39 -0.22660038192595522 1102 40-41 -0.19310117534092086 1102 42-43 0.3049135156207683 1102 44-45 0.3926747900759082 1102 46-47 0.7511888138474347 1102 48-49 1.2173163936239746 1102 50-51 0.5885927539345417 1102 52-53 1.0893372787974087 1102 54-55 0.8768199434149224 1102 56-57 1.1990684558637312 1102 58-59 1.2116872853987033 1102 60-61 1.38213288789483 1102 62-63 1.506180412000294 1102 64-65 1.2686715094600025 1102 66-67 2.1367512365578065 1102 68-69 2.5271574946019904 1102 70-71 2.089018144486154 1102 72-73 1.7117520832002349 1102 74-75 1.334084426485564 1102 76-77 1.2987899927443927 1102 78-79 0.6840426580188641 1102 80-81 1.2695089128839818 1102 82-83 1.3214052544984476 1102 84-85 1.8655866790206055 1102 86-87 2.014431537355165 1102 88-89 1.0263579074457674 1102 90-91 1.3382653489686192 1102 92-93 1.9021017997476015 1102 94-95 2.6855216730314915 1102 96-97 2.9606722866661705 1102 98-99 2.6883619878428675 1102 100-101 2.777560169336329 1103 1 0.37484234459443755 1103 2 0.5715523665480049 1103 3 0.4937609393192588 1103 4 0.4627677603374494 1103 5 0.1914646759730445 1103 6 0.3021337645695681 1103 7 0.28996312547060654 1103 8 0.493718816991894 1103 9 0.2578025618085249 1103 10-11 0.3256099051627501 1103 12-13 0.028837621707175742 1103 14-15 0.23467469614460867 1103 16-17 0.15857503393774408 1103 18-19 0.0669407223941576 1103 20-21 0.05680317676616653 1103 22-23 0.19915038948859376 1103 24-25 0.4258754925807082 1103 26-27 0.48981244433602456 1103 28-29 0.8990885959604995 1103 30-31 0.6926440029512122 1103 32-33 0.948759537752629 1103 34-35 0.920877733824021 1103 36-37 0.978339559368294 1103 38-39 1.6350067609311907 1103 40-41 1.311363110373371 1103 42-43 2.041520658477914 1103 44-45 2.205174790075908 1103 46-47 2.603867385276004 1103 48-49 1.6771378221954052 1103 50-51 1.9993070396488264 1103 52-53 2.4821944216545475 1103 54-55 2.492891371986353 1103 56-57 2.328532741578023 1103 58-59 1.9081158568272798 1103 60-61 2.06963288789483 1103 62-63 2.4079661262860093 1103 64-65 2.835635795174287 1103 66-67 2.145679807986383 1103 68-69 2.5762646374591327 1103 70-71 2.4506252873432963 1103 72-73 2.7028235117716655 1103 74-75 3.0572987121998487 1103 76-77 2.4461114213158233 1103 78-79 2.54564980087601 1103 80-81 2.769508912883982 1103 82-83 2.691940968784163 1103 84-85 3.0084438218777514 1103 86-87 2.91621725164088 1103 88-89 2.3165364788743403 1103 90-91 1.2891582061114732 1103 92-93 2.053887514033317 1103 94-95 2.261414530174349 1103 96-97 2.7285294295233093 1103 98-99 2.1124691307000134 1103 100-101 2.8311315979077563 1104 1 0.09853071374740807 1104 2 0.18541140573890402 1104 3 0.23182983944568036 1104 4 -0.07926763789264157 1104 5 -0.2589145907779056 1104 6 -0.17590036943801834 1104 7 0.08752949715201908 1104 8 0.00612400030415472 1104 9 0.30702822552533604 1104 10-11 0.4542050379061138 1104 12-13 0.382069290480878 1104 14-15 0.4350934698487805 1104 16-17 0.13483135505026667 1104 18-19 0.5294091168315802 1104 20-21 0.49714767739827437 1104 22-23 0.5150084805126127 1104 24-25 0.1477465418853825 1104 26-27 0.359795061276607 1104 28-29 0.7722712761122139 1104 30-31 1.083920867679403 1104 32-33 0.9904788803569247 1104 34-35 0.4906075062639701 1104 36-37 0.2891012534264519 1104 38-39 0.23871251314358233 1104 40-41 0.6060454744694468 1104 42-43 0.46519322484959247 1104 44-45 0.13094122496844562 1104 46-47 0.18580480626209805 1104 48-49 0.6831428790854233 1104 50-51 0.6482482533024339 1104 52-53 0.18668241154076526 1104 54-55 -0.14945218047888176 1104 56-57 0.22719740655904985 1104 58-59 0.022646514222977032 1104 60-61 0.41828175009458235 1104 62-63 0.16104766863746178 1104 64-65 0.7973930644536829 1104 66-67 0.9331719065957316 1104 68-69 0.7621053454237341 1104 70-71 0.5797340104785711 1104 72-73 1.1304072665124991 1104 74-75 1.0612099005690006 1104 76-77 1.0650668574725835 1104 78-79 0.5366027085877647 1104 80-81 0.8868445639585687 1104 82-83 1.1314163164453497 1104 84-85 1.6211176524719342 1104 86-87 0.8171733199088962 1104 88-89 1.0733316748288289 1104 90-91 0.8521717965033844 1104 92-93 1.86863941036707 1104 94-95 1.192474896798874 1104 96-97 1.0542642588532694 1104 98-99 2.2066142002322486 1104 100-101 2.6714444929772903 1105 1 -0.03587194111984715 1105 2 1.2379511943549915E-4 1105 3 0.1276895107478282 1105 4 0.052053474623164675 1105 5 0.1914646759730445 1105 6 0.14141947885527628 1105 7 0.30782026832775244 1105 8 0.09193310270617872 1105 9 0.1953025618085249 1105 10-11 0.4416813337341807 1105 12-13 0.34133762170717574 1105 14-15 0.3150318390017546 1105 16-17 0.2835750339377512 1105 18-19 0.6606907223941576 1105 20-21 0.04787460533759713 1105 22-23 0.49825753234573256 1105 24-25 0.2785540640092776 1105 26-27 0.40499101576459395 1105 28-29 0.4571243102462219 1105 30-31 0.8355011458083581 1105 32-33 0.4532238234669066 1105 34-35 0.7914134481097364 1105 36-37 1.0854824165111552 1105 38-39 1.5233996180740519 1105 40-41 1.6729702532305097 1105 42-43 1.621877801335053 1105 44-45 0.8569605043616164 1105 46-47 0.8092245281331429 1105 48-49 0.944994965052544 1105 50-51 1.3385927539345417 1105 52-53 0.991122993083124 1105 54-55 0.3812842291292071 1105 56-57 0.9981755987208771 1105 58-59 1.091151571112995 1105 60-61 1.274990030751976 1105 62-63 0.5106446977145787 1105 64-65 0.9561715094600025 1105 66-67 0.9492512365578065 1105 68-69 0.9334074946019904 1105 70-71 0.3434824302004422 1105 72-73 1.5197877974859502 1105 74-75 1.5572987121998487 1105 76-77 1.044325707030108 1105 78-79 0.6885069437331488 1105 80-81 0.6623660557411206 1105 82-83 0.27229811164130524 1105 84-85 -0.01834189240796391 1105 86-87 -0.08378274835911981 1105 88-89 0.3879650503029133 1105 90-91 1.2132653489686192 1105 92-93 0.9779946568904592 1105 94-95 0.9533788158886338 1105 96-97 0.514243715237594 1105 98-99 0.6615762735571522 1105 100-101 1.2373815979077563 1106 1 0.38692217652721084 1106 2 0.16451455142195925 1106 3 0.36613488889909007 1106 4 0.34722154185005394 1106 5 0.07171677681337485 1106 6 0.5111673780149459 1106 7 0.49059337757145016 1106 8 0.7568490690927376 1106 9 0.7278655870186057 1106 10-11 0.5207254513812387 1106 12-13 0.5238481259088559 1106 14-15 0.5416599902622607 1106 16-17 0.7570519246940464 1106 18-19 0.296457529116843 1106 20-21 0.8955636809678467 1106 22-23 0.6558205575558205 1106 24-25 0.5477242320765043 1106 26-27 0.12137757038644992 1106 28-29 0.6275549825151288 1106 30-31 0.44054316261507864 1106 32-33 1.011784747836657 1106 34-35 1.2373168094542706 1106 36-37 1.2414698114691376 1106 38-39 0.744513063452203 1106 40-41 0.6566887406254693 1106 42-43 1.08458788536867 1106 44-45 0.5898911766305233 1106 46-47 0.3249808306541553 1106 48-49 1.0910033684138938 1106 50-51 0.733025527043786 1106 52-53 0.45908517795707127 1106 54-55 0.5803388509779452 1106 56-57 0.3734382037628947 1106 58-59 0.5517608148104713 1106 60-61 0.16443330806290035 1106 62-63 0.3633232691431516 1106 64-65 0.2284929380314331 1106 66-67 0.47682476596957457 1106 68-69 -0.091802589431623 1106 70-71 -0.26681168744661576 1106 72-73 -0.2197080008333785 1106 74-75 -0.4755269180522532 1106 76-77 -0.05310076355812754 1106 78-79 0.02753005297684652 1106 80-81 -0.1900499106454312 1106 82-83 -0.517092644661215 1106 84-85 -0.3224385310634297 1106 86-87 -0.3933940928969335 1106 88-89 -0.34207696650381436 1106 90-91 -0.9096338106952473 1106 92-93 -0.8455347548742473 1106 94-95 -1.2640581589012818 1106 96-97 -1.127300402409464 1106 98-99 -0.6987178440899058 1106 100-101 0.38732907689935203 1107 1 -0.18708161853920302 1107 2 -0.04624717262250044 1107 3 -0.0480017334918017 1107 4 0.11599356678906503 1107 5 0.05667204924493063 1107 6 0.04234113784145421 1107 7 0.019801835148022917 1107 8 0.2503432409550257 1107 9 -0.07485872851405873 1107 10-11 0.018150227743397807 1107 12-13 0.06901619313574514 1107 14-15 0.03522193116765493 1107 16-17 0.3747328680391391 1107 18-19 0.22909510027434266 1107 20-21 0.07379626432376796 1107 22-23 0.23385661068675034 1107 24-25 0.20698148336411748 1107 26-27 0.05994493281529145 1107 28-29 -0.4393330630256713 1107 30-31 -0.22210254082758496 1107 32-33 0.009819445586721542 1107 34-35 0.4737291163124979 1107 36-37 0.4895722782623082 1107 38-39 0.28924063189893445 1107 40-41 0.2395025112950222 1107 42-43 -0.03105998668338117 1107 44-45 0.20791096519111107 1107 46-47 0.35069918251102195 1107 48-49 0.45248344431522014 1107 50-51 1.0474061179898442 1107 52-53 0.8393372787974087 1107 54-55 0.4443602659955701 1107 56-57 -0.20300527685515846 1107 58-59 0.4266930457673723 1107 60-61 0.8258252842081966 1107 62-63 0.9335997668390057 1107 64-65 0.4214652882618459 1107 66-67 0.26131920890803784 1107 68-69 0.4835227019752608 1107 70-71 0.3745884209838515 1107 72-73 0.13269102329240212 1107 74-75 0.38635977210768147 1107 76-77 0.509043448965592 1107 78-79 0.7047799852078036 1107 80-81 0.21795361334480745 1107 82-83 0.6107197706274796 1107 84-85 0.9905866790206055 1107 86-87 0.8774787723782111 1107 88-89 0.9997162023766428 1107 90-91 0.13146811394557645 1107 92-93 0.3063356707153453 1107 94-95 0.8422037006812602 1107 96-97 1.0083393373574125 1107 98-99 1.9121523104235152 1107 100-101 2.3711661601197385 1108 1 -0.27179536454326936 1108 2 -0.24255638506074462 1108 3 -0.15850740044907852 1108 4 -0.12957458200489214 1108 5 0.10040933491769977 1108 6 -0.022029684593881882 1108 7 0.0455937561012405 1108 8 0.11622525199832978 1108 9 0.0725548140607799 1108 10-11 -0.29178874348589545 1108 12-13 -0.3397675906480373 1108 14-15 0.11172585444576555 1108 16-17 0.45752130163401716 1108 18-19 -0.04800458005114905 1108 20-21 0.07248850495149384 1108 22-23 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10 358.0 11 514.0 12 695.0 13 938.0 14 1094.0 15 1408.0 16 1790.0 17 2326.0 18 2179.0 19 1620.0 20 1448.0 21 1557.0 22 1640.0 23 1691.0 24 1857.0 25 1910.0 26 1769.0 27 1865.0 28 2148.0 29 2550.0 30 3108.0 31 3712.0 32 4514.0 33 5500.0 34 7073.0 35 9762.0 36 13660.0 37 20509.0 38 16089.0 39 1440.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.30452076472349 21.448184423249646 16.229417048030452 22.017877763996406 2 15.585391043094546 22.299229265711286 39.26391888085626 22.85146081033791 3 18.301311869990865 26.55405809100298 34.08728160394671 21.05734843505945 4 12.984696272650456 20.450491204411026 34.75559273136965 31.809219791568864 5 12.179191639772553 38.00095623516555 34.84794153304988 14.971910592012023 6 27.56898862210538 37.102349838251236 18.111593844157838 17.217067695485543 7 23.41393467109362 36.19293109481824 21.068444277532624 19.32468995655551 8 24.33574312271148 34.204214713087126 21.542151398502916 19.917890765698484 9 24.069442903355213 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31.633820127858247 20.431286861668983 34-35 19.39541116820595 28.18341669938713 32.84224183553272 19.5789302968742 36-37 20.855368461489213 27.504535074802057 31.51033997054869 20.129756493160038 38-39 20.20026121027427 26.401871153337257 32.55823879399387 20.839628842394596 40-41 19.679470763977804 26.712334613743067 33.17883055911225 20.42936406316688 42-43 20.183012449797484 27.369067993734554 32.22862898579178 20.219290570676183 44-45 19.316979070640855 27.896138482023968 31.83148963774796 20.955392809587217 46-47 20.734377200506998 26.729571826682204 30.273001566227524 22.263049406583278 48-49 20.442993406312016 26.69398032563003 30.53709749695922 22.325928771098734 50-51 20.179496590162255 27.610723704987155 30.675310043444494 21.5344696614061 52-53 19.666527257363796 28.17618490794718 31.54462662490078 20.612661209788243 54-55 19.259821954404625 28.30464062273282 30.27543295123804 22.160104471624518 56-57 20.276204661167043 29.02496169751493 28.904186173549736 21.7946474677683 58-59 19.032438407142337 29.25772764711656 30.215388292129177 21.494445653611926 60-61 19.708253489989886 29.920661679036158 28.873771408329883 21.49731342264407 62-63 19.153376380063076 30.34282324523406 28.82608751317648 21.67771286152638 64-65 19.235906912430593 30.26327581865592 28.83700286364199 21.66381440527149 66-67 18.869285397248113 30.197770494042132 28.7432995322476 22.189644576462154 68-69 18.71356509418663 29.839707752579788 28.908937274348972 22.537789878884613 70-71 19.087836396070365 29.229009653382953 28.331526702571676 23.35162724797501 72-73 19.175749609511698 28.800112665477418 28.22526267273239 23.79887505227849 74-75 19.0891166855865 28.768958953918112 28.31146883348555 23.830455527009843 76-77 19.28443939639786 28.338471523608632 28.500730163881226 23.876358916112284 78-79 19.042755060175054 28.20192833698031 28.655805525164112 24.099511077680525 80-81 18.716604926680212 28.307072500384095 28.50552245685314 24.470800116082554 82-83 18.780540891185872 27.969801593567688 28.30652492136721 24.943132593879234 84-85 18.99352173504835 27.5018990961156 28.307627964937137 25.19695120389891 86-87 19.19109072035473 27.461308112587734 28.28704830985976 25.06055285719778 88-89 19.33295647258338 26.71428816143344 28.354917176285184 25.597838189697995 90-91 18.784516184314818 26.75049806130974 28.587648079518434 25.87733767485701 92-93 18.573160010583727 26.968018367886927 28.486868497196166 25.97195312433318 94-95 18.486100323486486 26.79048488831608 28.408344073539833 26.315070714657608 96-97 18.54029924633624 26.237399817345363 28.21416683025922 27.008134106059185 98-99 18.610288406551668 26.38505987487304 28.127960669506063 26.87669104906923 100-101 18.673580436591767 25.819943238802466 28.77878070119284 26.727695623412927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 380.0 1 256.5 2 133.5 3 136.0 4 127.0 5 120.0 6 174.0 7 276.5 8 350.5 9 404.5 10 385.5 11 306.0 12 281.0 13 290.0 14 316.5 15 332.0 16 338.5 17 363.0 18 407.5 19 425.5 20 451.5 21 516.0 22 571.0 23 637.0 24 732.0 25 840.0 26 966.5 27 1162.0 28 1341.5 29 1467.0 30 1622.5 31 1878.0 32 2083.0 33 2200.5 34 2404.0 35 2593.0 36 2784.0 37 2959.0 38 3052.5 39 3090.0 40 3141.0 41 3310.5 42 3504.5 43 3745.5 44 4086.5 45 4441.5 46 4732.0 47 4862.5 48 4945.0 49 4803.0 50 4538.0 51 4235.0 52 3794.0 53 3457.0 54 3129.5 55 2860.5 56 2571.0 57 2227.0 58 1994.0 59 1752.0 60 1406.5 61 1094.0 62 893.5 63 688.0 64 501.0 65 379.5 66 271.5 67 206.0 68 160.0 69 105.0 70 82.5 71 68.0 72 43.5 73 36.0 74 32.5 75 27.0 76 22.0 77 14.5 78 8.5 79 7.5 80 5.0 81 5.0 82 3.5 83 1.5 84 1.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21850274408719625 2 0.0 3 0.0 4 0.0 5 0.029873422043171362 6 0.0034141053763624414 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002987342204317136 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012802895161359155 26-27 0.0021338158602265257 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005974684408634272 36-37 0.01579023736567629 38-39 0.011949368817268545 40-41 0.009388789784996713 42-43 0.008962026612951408 44-45 0.004267631720453051 46-47 4.267631720453052E-4 48-49 0.002987342204317136 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012802895161359155 58-59 4.267631720453052E-4 60-61 0.003840868548407747 62-63 0.0012802895161359155 64-65 0.0021338158602265257 66-67 0.004267631720453051 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.05462568602179906 78-79 0.14424595215131317 80-81 0.002560579032271831 82-83 0.0021338158602265257 84-85 0.0 86-87 0.0977287663983749 88-89 0.34738522204487843 90-91 0.3913418287655448 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.002987342204317136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 117161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.38838009235155 #Duplication Level Percentage of deduplicated Percentage of total 1 90.59656552956358 70.111214482635 2 6.48181848261258 10.032348648441033 3 1.3598914733811998 3.1571939467911676 4 0.5878525185013621 1.8197181656011814 5 0.2669048958298867 1.0327668763496385 6 0.18970100034190296 0.8808391871015099 7 0.11580584323197564 0.6273418629065987 8 0.07830680828066924 0.48480296344346663 9 0.04632233729279026 0.3226329580662507 >10 0.23933207601274964 3.2997328462542996 >50 0.015440779097596752 0.827920553767892 >100 0.015440779097596752 2.5767960328095527 >500 0.005514563963427412 3.268152371522947 >1k 0.0011029127926854823 1.5585391043094545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1826 1.5585391043094545 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 948 0.8091429741978986 TruSeq Adapter, Index 11 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 920 0.7852442365633615 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 823 0.7024521811865723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 589 0.5027270166693695 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 549 0.46858596290574506 TruSeq Adapter, Index 11 (96% over 28bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 493 0.42078848763667087 RNA PCR Primer, Index 11 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 470 0.40115738172258686 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.3140976946253446 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.22106332311946805 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.18606874301175305 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 200 0.17070526881812206 TruSeq Adapter, Index 11 (96% over 26bp) TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 175 0.1493671102158568 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 140 0.11949368817268545 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 137 0.11693310914041362 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 136 0.11607958279632302 TruSeq Adapter, Index 11 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.267631720453052E-4 0.0 18-19 0.0 0.0 0.0 0.002560579032271831 0.0 20-21 0.0 0.0 0.0 0.003414105376362441 0.0 22-23 0.0 0.0 0.0 0.009815552957042019 0.0 24-25 0.0 0.0 0.0 0.018777579569993427 0.0 26-27 0.0 0.0 0.0 0.03670163279589624 0.0 28-29 0.0 0.0 0.0 0.0827920553767892 0.0 30-31 0.0 0.0 0.0 0.17369261102243921 0.0 32-33 0.0 0.0 0.0 0.3465316957007878 0.0 34-35 0.0 0.0 0.0 0.6392912317238671 0.0 36-37 0.0 0.0 0.0 1.0118554809194187 0.0 38-39 0.0 0.0 0.0 1.3899676513515589 0.0 40-41 0.0 0.0 0.0 1.7403402156007546 0.0 42-43 0.0 0.0 0.0 2.1261341231297104 0.0 44-45 0.0 0.0 0.0 2.641664034960439 0.0 46-47 0.0 0.0 0.0 3.1687165524363907 0.0 48-49 0.0 0.0 0.0 3.618098172600097 0.0 50-51 0.0 0.0 0.0 4.070040371796075 0.0 52-53 0.0 0.0 0.0 4.5330784134652315 0.0 54-55 0.0 0.0 0.0 5.057570351908911 0.0 56-57 0.0 0.0 0.0 5.685765741159601 0.0 58-59 0.0 0.0 0.0 6.357490973958911 0.0 60-61 0.0 0.0 0.0 7.03305707530663 0.0 62-63 0.0 0.0 0.0 7.667227148965953 0.0 64-65 0.0 0.0 0.0 8.365838461604117 0.0 66-67 0.0 0.0 0.0 9.115234591715673 0.0 68-69 0.0 0.0 0.0 9.943581908655611 0.0 70-71 0.0 0.0 0.0 10.781318015380545 0.0 72-73 0.0 0.0 0.0 11.64978107049274 0.0 74-75 0.0 0.0 0.0 12.547264021304017 0.0 76-77 0.0 0.0 0.0 13.554851870502983 0.0 78-79 0.0 0.0 0.0 14.594873720777391 0.0 80-81 0.0 0.0 0.0 15.678425414600422 0.0 82-83 0.0 0.0 0.0 16.77776734578913 0.0 84-85 0.0 0.0 0.0 17.90271506730055 0.0 86-87 0.0 0.0 0.0 19.098078712199452 0.0 88-89 0.0 0.0 0.0 20.24905898720564 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACC 25 0.005037141 56.92241 6 ATGTGTA 65 0.0058618416 29.190979 4 CTGTGTA 65 0.0058618416 29.190979 9 GTGTAGA 70 0.008347207 27.152262 1 GGCCAAC 35 0.008416485 27.117481 66-67 CTTTACA 90 8.881396E-4 26.398033 1 ACAGTCA 95 0.0012297154 24.96597 8 GAGTACT 840 0.0 22.305904 12-13 GGTATCA 1280 0.0 21.530897 1 CATGGGG 265 7.8398443E-10 21.480152 4 ATGGGGA 135 3.802761E-4 21.082375 5 GTATCAA 1660 0.0 20.323305 1 AGTACTT 865 0.0 20.29026 12-13 ACATGGG 580 0.0 19.628416 3 GTACTTT 950 0.0 19.223795 14-15 GTACATG 625 0.0 19.006584 1 TATCAAC 1775 0.0 18.974136 2 ATCAACG 1800 0.0 18.710606 3 TCAACGC 1840 0.0 18.561655 4 GACACAT 65 0.007586427 18.252151 20-21 >>END_MODULE