FastQCFastQC Report
Thu 26 May 2016
SRR938845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155786
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17471.1214101395504088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16541.061712862516529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.6316357053907283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.4364962191724545No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.38899516002721685No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.2792291990294378No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.20412617308358905No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.13865174020772084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGAG753.085433E-431.6331185
CCAAGAC953.496877E-529.968223
AGGGTGT1004.972604E-528.4606556
GGTATCA15250.028.3233431
GTATCAA21650.028.2816521
GCCGTAT605.3422427E-627.7235594-95
TCCAAGA1205.386746E-627.678982
GTGTTGC700.00839985427.1228241
TGTTGGC700.00841304927.1141012
ACAGGTG700.00841304927.1141015
ATGGGAG2550.026.0508045
TATCAAC24800.024.4901562
ATCAACG25150.023.7720053
ATGGGGG1401.7812306E-523.7248385
CATGGGA7500.023.4085064
TCAACGC25650.023.1236254
TACATGG10950.022.966512
GTACATG11100.022.663441
ACATGGG10700.022.6162013
GTATTAA1050.00219715722.6023541
Produced by FastQC (version 0.11.5)