FastQCFastQC Report
Thu 26 May 2016
SRR938845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155786
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17491.1226939519597396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16371.0508004570372178No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT15350.9853260241613495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.6573119535773433No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT9600.6162299564787593RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7080.45446959290308503No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.3453455381099714No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT4970.319027383718691RNA PCR Primer, Index 46 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.3023378223973913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.2291605150655386No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG2200.14121936502638235RNA PCR Primer, Index 46 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.12581361611441336No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1800.11554311683976738RNA PCR Primer, Index 46 (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.1123335858164405No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1650.10591452376978674No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG200.002077599371.1902853
ACGTTAA250.005029813456.9522366
TACGCCT250.005029813456.9522367
GGTATCA15900.032.897461
ATAGGTT700.00840400227.1201118
GACCGTC350.00841669527.12011148-49
GTATCAA21800.025.0846581
GAGTACT14850.022.2120112-13
TAAACAG1100.002880310421.5728155
GTCCAAA550.002886718821.57281546-47
ATCAACG25950.021.2153473
TATCAAC26000.021.1745472
GTACTTT15650.020.9249414-15
TCAACGC26600.020.6969264
CAACGCA26650.020.6580965
AACGCAG26850.020.3274576
AGTACTT15250.019.9177212-13
GTGAGCC1200.00476843919.775084
GGTGAAG1200.00476843919.775088
CGCAGAG28950.018.8529268
Produced by FastQC (version 0.11.5)