##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938845_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155786 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68454161477925 31.0 31.0 34.0 30.0 34.0 2 31.900665014828032 31.0 31.0 34.0 30.0 34.0 3 31.93462827211688 33.0 31.0 34.0 30.0 34.0 4 35.67741645590746 37.0 35.0 37.0 33.0 37.0 5 35.583544092537196 37.0 35.0 37.0 33.0 37.0 6 35.63800983400306 37.0 35.0 37.0 33.0 37.0 7 35.559106723325584 37.0 35.0 37.0 33.0 37.0 8 35.54548547366259 37.0 35.0 37.0 33.0 37.0 9 37.08781276879822 39.0 37.0 39.0 33.0 39.0 10-11 37.031889900247776 39.0 37.0 39.0 33.0 39.0 12-13 36.95001797337373 39.0 37.0 39.0 33.0 39.0 14-15 37.98729025714762 40.0 37.0 41.0 33.0 41.0 16-17 37.90965491122437 40.0 37.0 41.0 32.5 41.0 18-19 37.99142413310567 40.0 37.0 41.0 33.0 41.0 20-21 38.111149268868836 40.0 37.0 41.0 33.0 41.0 22-23 38.02882158858948 40.0 37.0 41.0 33.0 41.0 24-25 37.90238532345654 40.0 37.0 41.0 33.0 41.0 26-27 37.611476641033214 40.0 36.5 41.0 32.0 41.0 28-29 37.324011785397914 39.5 36.0 41.0 32.0 41.0 30-31 36.89888693464111 39.0 35.5 41.0 31.0 41.0 32-33 36.39984979394811 38.5 35.0 40.5 30.0 41.0 34-35 36.088339131886045 38.0 35.0 41.0 30.0 41.0 36-37 35.778054510674906 38.0 35.0 41.0 29.5 41.0 38-39 35.464592453750654 38.0 35.0 41.0 27.5 41.0 40-41 35.12286084757295 38.0 35.0 40.0 26.0 41.0 42-43 34.703506733596086 38.0 34.0 40.0 24.5 41.0 44-45 34.17179656708562 38.0 33.5 40.0 21.5 41.0 46-47 33.424007933960695 38.0 33.0 40.0 11.5 41.0 48-49 32.80661612725149 38.0 33.0 40.0 2.0 41.0 50-51 32.27974272399317 37.5 32.0 40.0 2.0 41.0 52-53 31.97896794320414 37.0 31.5 40.0 2.0 41.0 54-55 31.644743430089996 37.0 31.0 40.0 2.0 41.0 56-57 31.244139396351407 36.5 31.0 40.0 2.0 41.0 58-59 30.81975915679201 36.0 30.0 40.0 2.0 41.0 60-61 30.335787554722504 35.5 29.0 39.0 2.0 41.0 62-63 29.833954912508183 35.0 28.0 39.0 2.0 40.5 64-65 29.329849922329352 35.0 28.0 39.0 2.0 40.0 66-67 28.780246620363833 35.0 26.5 38.0 2.0 40.0 68-69 28.243975710269215 34.5 26.0 37.5 2.0 40.0 70-71 27.728521818391897 34.0 24.5 37.0 2.0 39.5 72-73 27.369478001874366 34.0 24.0 36.5 2.0 39.0 74-75 26.93754252628606 34.0 22.5 36.0 2.0 39.0 76-77 25.072503305816955 31.5 19.5 34.5 2.0 36.0 78-79 25.981368672409587 33.0 20.0 35.0 2.0 37.0 80-81 25.98636912174393 33.0 20.0 35.0 2.0 37.0 82-83 25.64871361996585 33.0 18.0 35.0 2.0 36.5 84-85 25.232665322943014 33.0 12.0 35.0 2.0 36.0 86-87 24.672563645000196 32.5 4.5 35.0 2.0 35.5 88-89 24.13899836955824 32.0 2.0 35.0 2.0 35.0 90-91 23.672191339401486 32.0 2.0 35.0 2.0 35.0 92-93 23.337215796027884 31.0 2.0 34.0 2.0 35.0 94-95 22.893594417983643 31.0 2.0 34.0 2.0 35.0 96-97 22.32935244502073 31.0 2.0 34.0 2.0 35.0 98-99 21.55531947671806 31.0 2.0 34.0 2.0 35.0 100-101 18.981233872106607 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13641637472146328 1101 2 0.13553512125557887 1101 3 0.10242443985501026 1101 4 0.10036149770250802 1101 5 -0.05946590106969296 1101 6 0.12266036939819003 1101 7 0.20405569429220805 1101 8 0.06713974624219077 1101 9 0.5312882561416714 1101 10-11 0.22217974482884983 1101 12-13 0.3612931685953953 1101 14-15 0.4082976194621537 1101 16-17 0.12292492276889533 1101 18-19 0.174409310960975 1101 20-21 0.004853696497868043 1101 22-23 0.39587679003359 1101 24-25 0.6057459038783151 1101 26-27 0.7660644772280207 1101 28-29 0.5441979576398808 1101 30-31 0.2553654571763815 1101 32-33 0.635112926655232 1101 34-35 0.47186225167420304 1101 36-37 0.14026622497680563 1101 38-39 -0.13157343403186417 1101 40-41 0.10438863742482596 1101 42-43 -0.28348211154445124 1101 44-45 0.07177827022130856 1101 46-47 0.2899777708649296 1101 48-49 0.24328588496180004 1101 50-51 0.05727961605558107 1101 52-53 -0.7214541804964725 1101 54-55 -0.8240041258056365 1101 56-57 -1.1715663093089788 1101 58-59 -0.5408625903488655 1101 60-61 -0.43002718265766404 1101 62-63 -0.3818664781383454 1101 64-65 -0.4857758662604752 1101 66-67 -0.0381092868601165 1101 68-69 -0.6369517871371109 1101 70-71 -0.3017947053046868 1101 72-73 -0.18896238225857687 1101 74-75 -0.09191637038911438 1101 76-77 0.18635623670522605 1101 78-79 0.2248389959786472 1101 80-81 0.3653083801829986 1101 82-83 0.019874108483538322 1101 84-85 -0.22894375354287178 1101 86-87 -0.22274676140039773 1101 88-89 0.24239875979234426 1101 90-91 0.37309356249665626 1101 92-93 0.2458543058019309 1101 94-95 -0.20886600173743375 1101 96-97 0.1682723817654086 1101 98-99 0.6771556995025669 1101 100-101 1.473771305672983 1102 1 0.1927525121834286 1102 2 0.10161709727132973 1102 3 0.14824509494718896 1102 4 0.14466076385607352 1102 5 -0.21563200094440305 1102 6 0.2599433477407729 1102 7 0.221327933418749 1102 8 -0.3560748590916205 1102 9 -0.2427854864751211 1102 10-11 0.23059213072468054 1102 12-13 0.22935742490467703 1102 14-15 -0.03386722750401816 1102 16-17 0.3774890895847136 1102 18-19 0.16098529091443226 1102 20-21 0.3764753181194891 1102 22-23 0.45641912044346356 1102 24-25 0.3707136862302036 1102 26-27 0.24536911298961428 1102 28-29 0.2944888114075539 1102 30-31 0.22923336481912315 1102 32-33 0.7214070021877177 1102 34-35 0.3511131913556014 1102 36-37 0.554374870045713 1102 38-39 0.45436266763271504 1102 40-41 0.5829996988173463 1102 42-43 0.567981106640616 1102 44-45 0.5877752274434371 1102 46-47 -0.41601381674917803 1102 48-49 -0.9316514696113174 1102 50-51 -1.0469355262390323 1102 52-53 -0.5726041715471268 1102 54-55 -0.7336604708924455 1102 56-57 -0.9690873402737168 1102 58-59 -0.8555216202396849 1102 60-61 -1.095053135763088 1102 62-63 -1.6296067680971795 1102 64-65 -1.2190629613025337 1102 66-67 -1.097398708732321 1102 68-69 -1.6414488338526994 1102 70-71 -1.2919951706707167 1102 72-73 -1.2972494773006353 1102 74-75 -1.2372090140261243 1102 76-77 -0.9963223027613886 1102 78-79 -1.1617951547283525 1102 80-81 -0.8883832253297967 1102 82-83 -1.1309223833725568 1102 84-85 -1.3228671471351383 1102 86-87 -1.4858799276792602 1102 88-89 -1.4293168299696042 1102 90-91 -0.9864688144498288 1102 92-93 -0.7303404319822135 1102 94-95 -1.355210193611427 1102 96-97 -0.7331953110930094 1102 98-99 -0.2902463051510935 1102 100-101 0.13776808390817408 1103 1 0.3120920503971405 1103 2 0.5814810672015227 1103 3 0.1294514668820419 1103 4 0.30982095716196056 1103 5 0.21080436920058077 1103 6 0.46725496399278654 1103 7 0.3459475861840957 1103 8 0.39822082732327146 1103 9 0.4434504183038328 1103 10-11 0.5160986637477691 1103 12-13 0.5234553307575567 1103 14-15 0.577216538381073 1103 16-17 0.7310330308770006 1103 18-19 0.38386877042042755 1103 20-21 0.5791780208221908 1103 22-23 0.3452011143579128 1103 24-25 0.6935837417161537 1103 26-27 0.245794206957747 1103 28-29 0.520549308991221 1103 30-31 0.09320329501422009 1103 32-33 0.7161940077363198 1103 34-35 0.07659198140392931 1103 36-37 0.2314824411930161 1103 38-39 0.7062644038059673 1103 40-41 0.6246589076950997 1103 42-43 0.4394908614285171 1103 44-45 0.923129621572663 1103 46-47 0.7359237168108805 1103 48-49 0.8412588579347684 1103 50-51 0.8714688052447741 1103 52-53 0.7717890627467696 1103 54-55 0.8449147931132828 1103 56-57 0.7676228798802107 1103 58-59 0.73278605829978 1103 60-61 0.6544322768017992 1103 62-63 1.2904308191589529 1103 64-65 1.3351700796854722 1103 66-67 1.1713501725993396 1103 68-69 1.1839941588088365 1103 70-71 1.4313134028034185 1103 72-73 1.5475241042279109 1103 74-75 1.0398403863676435 1103 76-77 0.568112993461984 1103 78-79 0.9174065635462121 1103 80-81 0.8653083801829986 1103 82-83 1.34757681118624 1103 84-85 1.4163265167273984 1103 86-87 1.6961721575185216 1103 88-89 1.3944257868193723 1103 90-91 1.5825530219561124 1103 92-93 2.0431516030992256 1103 94-95 1.3519448090733803 1103 96-97 1.235839949332977 1103 98-99 0.8697232670701354 1103 100-101 0.9028253597270393 1104 1 0.01586160942985515 1104 2 0.18709984387293588 1104 3 0.10829214967008838 1104 4 -0.13701403572566306 1104 5 0.2292965455874949 1104 6 0.16195624422037724 1104 7 0.38044260752122483 1104 8 0.1820046111070539 1104 9 0.3239625093422376 1104 10-11 0.11380492263823783 1104 12-13 0.17557126247875487 1104 14-15 0.29521084848065016 1104 16-17 0.44804938934073135 1104 18-19 0.21912837212740754 1104 20-21 0.0014753181194890885 1104 22-23 0.11751619259403867 1104 24-25 0.3558348085724816 1104 26-27 0.5358013193332809 1104 28-29 0.017170930612849133 1104 30-31 0.13961154536984566 1104 32-33 0.5498533249482662 1104 34-35 0.17376481212939154 1104 36-37 -0.25420390304595486 1104 38-39 0.3331314023834935 1104 40-41 -0.25398618476578605 1104 42-43 -0.12496504184317558 1104 44-45 0.3155549416295642 1104 46-47 -0.23616020922042225 1104 48-49 -0.41825394434119545 1104 50-51 0.22735429599867985 1104 52-53 -0.17122302829163516 1104 54-55 -0.22407524956096836 1104 56-57 -0.25095820120087 1104 58-59 -0.09251621766038909 1104 60-61 -0.15628962931484836 1104 62-63 0.00709155884600321 1104 64-65 -0.18403333425478507 1104 66-67 -0.10567685442768493 1104 68-69 0.21235475624838074 1104 70-71 0.14539590635960664 1104 72-73 -0.17998300814761947 1104 74-75 0.08953811040747439 1104 76-77 0.16039606600821443 1104 78-79 0.1513148139018341 1104 80-81 -0.4685287464172845 1104 82-83 -0.40464580616795587 1104 84-85 -0.26343877487999734 1104 86-87 -0.014265253576784431 1104 88-89 0.10486319791467835 1104 90-91 0.48235743162894096 1104 92-93 0.35085074961416396 1104 94-95 0.3187833581487709 1104 96-97 0.6737844728038134 1104 98-99 1.0207723424613135 1104 100-101 1.6997670382476642 1105 1 -0.06379222313814026 1105 2 -0.12648192935207447 1105 3 0.38412224504275017 1105 4 0.19586303951277273 1105 5 0.14505069606435939 1105 6 -0.00322390413709428 1105 7 -0.04290603253529213 1105 8 0.21723951043590972 1105 9 -0.09037871284073162 1105 10-11 -0.007640679622440416 1105 12-13 0.07735066904886878 1105 14-15 0.03969159733264149 1105 16-17 0.06238619612278029 1105 18-19 -0.40730663317108196 1105 20-21 -0.14365891006843157 1105 22-23 0.033778114176520546 1105 24-25 -0.28013746270975304 1105 26-27 -0.19282814022164274 1105 28-29 0.007942742693387572 1105 30-31 -0.5174442652977689 1105 32-33 -0.3916646898874774 1105 34-35 -0.18637736378018843 1105 36-37 0.2074483399777094 1105 38-39 0.34935800075863455 1105 40-41 0.740340025054067 1105 42-43 -0.10547104678842345 1105 44-45 0.039150844137509466 1105 46-47 0.505627145071351 1105 48-49 0.4000743848711039 1105 50-51 0.4873993330880495 1105 52-53 0.5212222207369734 1105 54-55 0.7266488761896497 1105 56-57 -0.034934711267982976 1105 58-59 0.3796888335582622 1105 60-61 -0.5372283435500975 1105 62-63 -0.3581724821289143 1105 64-65 0.11550746404970269 1105 66-67 0.4362694542971468 1105 68-69 0.6903881366793243 1105 70-71 1.208227061428488 1105 72-73 1.4433612165079772 1105 74-75 1.0122465173308228 1105 76-77 0.08997042135968059 1105 78-79 0.33360464081811614 1105 80-81 0.657685488835277 1105 82-83 0.5181509087737588 1105 84-85 0.04246020074699075 1105 86-87 0.3472015426083104 1105 88-89 0.9069870057564309 1105 90-91 0.4127044821411303 1105 92-93 0.11743058006276641 1105 94-95 0.20254792897179996 1105 96-97 0.2049130492785629 1105 98-99 1.0416351118007725 1105 100-101 1.9508708884772616 1106 1 -0.2960160577109683 1106 2 -0.36446487874442113 1106 3 -0.4043323169017441 1106 4 0.12063176797277464 1106 5 -0.19460103620482272 1106 6 0.06860631534413386 1106 7 0.10946109969761153 1106 8 0.1887613678638118 1106 9 -0.03627931142589347 1106 10-11 -0.22714457949547295 1106 12-13 -0.11167980437758018 1106 14-15 -0.08156724540270943 1106 16-17 -0.03585886101489422 1106 18-19 0.025760662312322324 1106 20-21 0.07917802082219083 1106 22-23 -0.10412320996641711 1106 24-25 -0.03614598801357971 1106 26-27 -0.30825984709630205 1106 28-29 -0.1348560964141825 1106 30-31 0.03239248420341312 1106 32-33 -0.4088059922636873 1106 34-35 -0.3288134240014742 1106 36-37 -0.11649053177995228 1106 38-39 0.04072386326543409 1106 40-41 -0.15236811933193195 1106 42-43 -0.2868604899228373 1106 44-45 -0.1816001081570633 1106 46-47 0.14470750059466297 1106 48-49 -0.24320060152468415 1106 50-51 -0.45285551907955224 1106 52-53 -0.3599676940099883 1106 54-55 -0.2598149366164435 1106 56-57 -0.34386360660627346 1106 58-59 -0.6455923200785989 1106 60-61 -0.7171893448198254 1106 62-63 -0.8379475592194261 1106 64-65 -0.7256407311253383 1106 66-67 -0.6462173949682253 1106 68-69 -1.133573408758732 1106 70-71 -1.359227137737122 1106 72-73 -1.4727461660423593 1106 74-75 -1.6290785325512758 1106 76-77 -1.0433734930245002 1106 78-79 -1.0927285715889177 1106 80-81 -1.0536105387359207 1106 82-83 -1.020666432057002 1106 84-85 -0.8404302400293595 1106 86-87 -0.645044058697696 1106 88-89 -0.7238174564238697 1106 90-91 -0.5863658969628034 1106 92-93 0.18166511661273788 1106 94-95 0.17964751177608207 1106 96-97 0.6243534628464893 1106 98-99 0.8832367805836476 1106 100-101 1.2102577921594673 1107 1 0.05533529364038259 1107 2 0.32525773860977836 1107 3 0.10829214967008838 1107 4 0.17877543795854933 1107 5 0.25561233506117986 1107 6 0.017219402115117077 1107 7 -0.06034686616298757 1107 8 -0.08115328362978858 1107 9 -0.057616438026180106 1107 10-11 0.03156808053297766 1107 12-13 -0.04153400067914248 1107 14-15 0.41363190111222536 1107 16-17 0.3592335998670464 1107 18-19 0.27833889844318804 1107 20-21 0.16594900233001653 1107 22-23 0.20633198206772363 1107 24-25 0.27359796646721435 1107 26-27 -0.023409206982499597 1107 28-29 0.0369077727181093 1107 30-31 0.07711154536984566 1107 32-33 -0.20672562242015857 1107 34-35 0.15731744370832956 1107 36-37 0.23263820221719556 1107 38-39 -0.1701580713007118 1107 40-41 0.8019348678657963 1107 42-43 0.7039823265778793 1107 44-45 1.1379233626821943 1107 46-47 -0.11116020922042225 1107 48-49 -0.6189118390780308 1107 50-51 -0.525935177685529 1107 52-53 -0.3060914493442688 1107 54-55 -0.15170682850833828 1107 56-57 0.02535758827281498 1107 58-59 -0.06291095450249173 1107 60-61 -0.23523699773589968 1107 62-63 0.3788020851617979 1107 64-65 0.22715087627152997 1107 66-67 -0.10238738074347253 1107 68-69 -0.3929084016463591 1107 70-71 -0.5157883041667048 1107 72-73 -0.7293251134107805 1107 74-75 -0.5091461001188406 1107 76-77 -0.5567091971496794 1107 78-79 -0.8552641334665871 1107 80-81 -0.8073445358909694 1107 82-83 -0.9803037009047983 1107 84-85 -0.9969914064589425 1107 86-87 -0.8991336746294145 1107 88-89 -0.9115841705063765 1107 90-91 -0.4123794104763192 1107 92-93 -0.16559661880689092 1107 94-95 0.17404651604350363 1107 96-97 0.36457394648802577 1107 98-99 0.2345881319349985 1107 100-101 0.5945038803529279 1108 1 0.3786686269737132 1108 2 0.4083279140483711 1108 3 0.07539741282798573 1108 4 0.09675789409890001 1108 5 0.10684040523661764 1108 6 -0.18742972069190245 1108 7 -0.03990826967175565 1108 8 -0.18132872222628293 1108 9 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709.0 11 990.0 12 1298.0 13 1822.0 14 1959.0 15 2535.0 16 3121.0 17 3972.0 18 3590.0 19 2404.0 20 1928.0 21 1945.0 22 2121.0 23 2177.0 24 2321.0 25 2523.0 26 2532.0 27 2738.0 28 3110.0 29 3735.0 30 4356.0 31 4959.0 32 5963.0 33 7460.0 34 9455.0 35 12769.0 36 17478.0 37 24653.0 38 18802.0 39 1580.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.865484604629884 21.79577215238807 16.26129928032524 20.077443962656805 2 16.116339080533553 23.106055743134814 39.04779633599939 21.72980884033225 3 18.825183264221433 28.046165894239532 33.36243308127816 19.766217760260872 4 12.986404426585189 20.68478553913702 35.397917656272064 30.930892378005726 5 11.658942395337194 39.33408650328014 34.291913265633625 14.71505783574904 6 26.79059735791406 37.40451645205602 18.229494306291965 17.575391883737947 7 23.0283850923703 37.23762083884303 21.305508839048436 18.428485229738232 8 24.792985248995418 33.920249573132374 21.17905331672936 20.10771186114285 9 25.018936233037632 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18.68268008678572 34-35 19.187166187345934 27.559123356614627 33.95901807723911 19.29469237880033 36-37 19.04332361740649 28.231936722351342 33.67788492404885 19.046854736193325 38-39 19.181903392311444 26.95134947741455 34.23159394982152 19.63515318045248 40-41 18.88358567297168 27.305320798199972 34.41747900022789 19.39361452860045 42-43 19.291938823000756 27.58967116560479 33.571921490587535 19.54646852080692 44-45 18.73316964357357 28.14083738545729 32.86601511723076 20.259977853738384 46-47 19.743679448458256 27.507117340634156 31.686689369405226 21.062513841502362 48-49 19.62307267662049 27.660380265235645 31.314431335293285 21.402115722850574 50-51 19.504507829726318 28.09425841466898 31.235135716326077 21.16609803927862 52-53 19.27194998266853 28.546852733878524 31.686095027794536 20.495102255658402 54-55 18.862413824092023 28.614573838470726 30.922226644242745 21.60078569319451 56-57 19.758193932702554 29.29178488439269 29.431399483907413 21.518621698997343 58-59 18.992139833296424 29.50691816632485 30.50123406863925 20.999707931739476 60-61 19.35654702489657 29.932374530200374 29.68106583132468 21.03001261357837 62-63 18.805284171876803 30.15161824554196 29.794718395748017 21.24837918683322 64-65 18.684605805399716 30.325253873903947 29.617552283260366 21.372588037435968 66-67 18.61800464752025 30.094940365382396 29.340039285668436 21.94701570142892 68-69 18.613033263579524 29.883301451991834 29.314572554658312 22.189092729770326 70-71 18.86939608688954 29.454244338314588 28.93525650901248 22.741103065783392 72-73 18.797260344318488 29.1720693772226 28.813243808814654 23.217426469644256 74-75 18.89547199363229 29.09439872645809 28.654051070057644 23.356078209851976 76-77 18.958521407528536 28.852535909454524 29.045937758016375 23.14300492500056 78-79 18.46549498815265 28.79805562155255 29.08476796527345 23.65168142502135 80-81 18.387403232639645 29.023147137740235 28.828649557726628 23.760800071893495 82-83 18.704825850846674 28.6261281501547 28.485550691332982 24.183495307665645 84-85 18.586715109188244 28.38509237030285 28.34818276353459 24.68000975697431 86-87 18.64702592552118 28.30400935967241 28.442540225377826 24.606424489428587 88-89 18.79765584750129 27.95305255023184 28.406427099433284 24.842864502833592 90-91 18.538984130866446 27.877990145754016 28.668389496957154 24.91463622642238 92-93 18.487861553669777 28.148870886985993 28.33694940495295 25.02631815439128 94-95 18.510007317730732 27.848137830100267 28.33630749874828 25.30554735342072 96-97 18.604367529816543 27.520123759516256 28.028192520508906 25.847316190158292 98-99 18.631006637310158 27.48289319964567 27.96079236901904 25.925307794025137 100-101 18.703602068228427 27.268584046653892 28.57743499876433 25.450378886353352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 566.0 1 387.0 2 214.0 3 205.0 4 195.5 5 195.5 6 273.0 7 451.0 8 592.0 9 656.5 10 589.5 11 487.5 12 495.0 13 516.5 14 524.0 15 557.5 16 587.0 17 620.0 18 671.0 19 719.5 20 780.0 21 900.5 22 1029.0 23 1152.5 24 1309.5 25 1449.5 26 1624.5 27 1880.5 28 2126.5 29 2305.0 30 2512.5 31 2784.5 32 3059.0 33 3324.5 34 3535.0 35 3696.0 36 3928.0 37 4126.0 38 4231.0 39 4250.0 40 4264.0 41 4445.0 42 4644.0 43 4834.0 44 5120.5 45 5455.5 46 5650.0 47 5761.0 48 5951.5 49 5848.0 50 5465.0 51 4996.0 52 4483.0 53 4042.0 54 3632.5 55 3327.5 56 3037.5 57 2630.0 58 2321.0 59 2143.0 60 1874.5 61 1587.5 62 1288.0 63 942.5 64 683.5 65 514.0 66 379.5 67 284.0 68 213.5 69 176.5 70 142.0 71 106.5 72 81.5 73 63.0 74 50.5 75 35.5 76 26.0 77 19.0 78 14.0 79 8.0 80 4.5 81 4.0 82 3.0 83 2.0 84 1.5 85 2.5 86 2.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37037988009192097 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.419062046653743E-4 16-17 0.001604765511663436 18-19 3.2095310233268715E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.419062046653743E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006419062046653744 36-37 0.01797337373063048 38-39 0.014121936502638235 40-41 0.00674001514898643 42-43 0.005456202739655682 44-45 0.00224667171632881 46-47 0.0028885779209941844 48-49 0.0 50-51 9.628593069980615E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.2095310233268715E-4 60-61 9.628593069980615E-4 62-63 0.0 64-65 0.0 66-67 0.0025676248186614972 68-69 0.0 70-71 0.0012838124093307486 72-73 0.0 74-75 0.0 76-77 0.09724879000680421 78-79 0.03530484125659559 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1444288960497092 88-89 0.3248045395606794 90-91 0.27056346526645525 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.2095310233268715E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 155786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.87306110665499 #Duplication Level Percentage of deduplicated Percentage of total 1 88.85226701668574 64.74936683771767 2 7.329271510950304 10.682129013694942 3 1.6714973499130092 3.6542138555946777 4 0.7649499007717768 2.2297696344988545 5 0.37023654110792636 1.349013504203725 6 0.24546512742452437 1.0732677138216133 7 0.14268625406929064 0.7278588878309781 8 0.10975828849855726 0.6398737971773786 9 0.09479119517987246 0.6216952102844135 >10 0.3671911145986786 4.551099270668895 >50 0.0279384542771 1.4048128731748104 >100 0.01696263295395357 2.39007241931967 >500 0.0029934058154035717 1.6040461688528116 >1k 0.003991207753871429 4.322780813159549 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1749 1.1226939519597396 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1637 1.0508004570372178 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1535 0.9853260241613495 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1024 0.6573119535773433 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 960 0.6162299564787593 RNA PCR Primer, Index 46 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 708 0.45446959290308503 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 538 0.3453455381099714 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 497 0.319027383718691 RNA PCR Primer, Index 46 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 471 0.3023378223973913 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 357 0.2291605150655386 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 220 0.14121936502638235 RNA PCR Primer, Index 46 (96% over 28bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 0.12581361611441336 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 180 0.11554311683976738 RNA PCR Primer, Index 46 (96% over 26bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.1123335858164405 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 165 0.10591452376978674 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.419062046653743E-4 0.0 14-15 0.0 0.0 0.0 6.419062046653743E-4 0.0 16-17 0.0 0.0 0.0 6.419062046653743E-4 0.0 18-19 0.0 0.0 0.0 6.419062046653743E-4 0.0 20-21 0.0 0.0 0.0 0.0025676248186614972 0.0 22-23 0.0 0.0 0.0 0.010591452376978675 0.0 24-25 0.0 0.0 0.0 0.017331467525965108 0.0 26-27 0.0 0.0 0.0 0.03145340402860334 0.0 28-29 0.0 0.0 0.0 0.0651534797735355 0.0 30-31 0.0 0.0 0.0 0.13929364641238623 0.0 32-33 0.0 0.0 0.0 0.2644653563221342 0.0 34-35 0.0 0.0 0.0 0.4942677775923382 0.0 36-37 0.0 0.0 0.0 0.8165046923343562 0.0 38-39 0.0 0.0 0.0 1.174688354537635 0.0 40-41 0.0 0.0 0.0 1.4953205037679895 0.0 42-43 0.0 0.0 0.0 1.824939339863659 0.0 44-45 0.0 0.0 0.0 2.243783138407816 0.0 46-47 0.0 0.0 0.0 2.7377299628978213 0.0 48-49 0.0 0.0 0.0 3.1780776192982683 0.0 50-51 0.0 0.0 0.0 3.626770056359365 0.0 52-53 0.0 0.0 0.0 4.071290103090137 0.0 54-55 0.0 0.0 0.0 4.57582837995712 0.0 56-57 0.0 0.0 0.0 5.154185870360623 0.0 58-59 0.0 0.0 0.0 5.755651984132079 0.0 60-61 0.0 0.0 0.0 6.373486706122502 0.0 62-63 0.0 0.0 0.0 6.957300399265659 0.0 64-65 0.0 0.0 0.0 7.59054087016805 0.0 66-67 0.0 0.0 0.0 8.26518429127136 0.0 68-69 0.0 0.0 0.0 9.040286033404799 0.0 70-71 0.0 0.0 0.0 9.835928774087531 0.0 72-73 0.0 0.0 0.0 10.675221136687508 0.0 74-75 0.0 0.0 0.0 11.505847765524502 0.0 76-77 0.0 0.0 0.0 12.363434454957442 0.0 78-79 0.0 0.0 0.0 13.333996636411488 0.0 80-81 0.0 0.0 0.0 14.357516079750427 0.0 82-83 0.0 0.0 0.0 15.342521150809443 0.0 84-85 0.0 0.0 0.0 16.393642560948994 0.0 86-87 0.0 0.0 0.0 17.438665862144223 0.0 88-89 0.0 0.0 0.0 18.504872068093412 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTAG 20 0.0020775993 71.190285 3 ACGTTAA 25 0.0050298134 56.952236 6 TACGCCT 25 0.0050298134 56.952236 7 GGTATCA 1590 0.0 32.89746 1 ATAGGTT 70 0.008404002 27.120111 8 GACCGTC 35 0.008416695 27.120111 48-49 GTATCAA 2180 0.0 25.084658 1 GAGTACT 1485 0.0 22.21201 12-13 TAAACAG 110 0.0028803104 21.572815 5 GTCCAAA 55 0.0028867188 21.572815 46-47 ATCAACG 2595 0.0 21.215347 3 TATCAAC 2600 0.0 21.174547 2 GTACTTT 1565 0.0 20.92494 14-15 TCAACGC 2660 0.0 20.696926 4 CAACGCA 2665 0.0 20.658096 5 AACGCAG 2685 0.0 20.327457 6 AGTACTT 1525 0.0 19.91772 12-13 GTGAGCC 120 0.004768439 19.77508 4 GGTGAAG 120 0.004768439 19.77508 8 CGCAGAG 2895 0.0 18.852926 8 >>END_MODULE