FastQCFastQC Report
Thu 26 May 2016
SRR938844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153770
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17331.127007868895103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16151.0502698835923783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.661377381803993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7220.46953241854718086No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.3836899265136242No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.3108538726669702No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.2315145997268648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.12681277232229954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.10730311504194576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTC459.638287E-442.187186
GGTATCA13950.032.0432471
GTTGTAG600.003918688731.7023641
AGGACGT600.003956433431.6403835
GAAGTCC600.003956433431.6403836
GTATCAA20350.030.1445081
TATCAAC23650.025.8875852
ATCAACG24050.025.6543663
TCAACGC23950.025.5633134
CAACGCA23900.025.4182155
AACGCAG24150.025.1550886
CCTGGAC950.001227644624.9792483
ATGGGAT2151.6498234E-924.2821545
ATGGGAC1201.711029E-423.7302885
ACGCAGA26350.023.0548527
GAGTACT14600.022.92507612-13
CGCAGAG26350.022.694628
GCAGAGT27050.021.7493329
AGTACTT14900.021.66691812-13
ATGGGAG2056.804312E-720.8363487
Produced by FastQC (version 0.11.5)