FastQCFastQC Report
Thu 26 May 2016
SRR938844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153770
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17061.1094491773427848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15741.023606685309228No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT12580.8181049619561682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.6730831761722053No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT9210.5989464785068609RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.4675814528191454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.35182415295571307No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.29264485920530664No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT4000.26012876373805033RNA PCR Primer, Index 46 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.21330558626520127No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG3030.19704753853157314RNA PCR Primer, Index 46 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.11770826559146777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.10925408076998115No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1580.10275086167652989No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1570.10210053976718476RNA PCR Primer, Index 46 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG459.505437E-442.3062971
GACAGCG459.643637E-442.1824767
CTTGGAC608.287304E-539.5589373
CTCCCGC500.00161893837.964237
GTATCCT550.002580498634.5241623
GGTATCA15850.032.7306631
GTTGCAC600.003952314631.647153
ATACTGG650.005772109629.2889771
GTGTAAG650.005772109629.2889771
TCTAGGT650.005846127429.2127532
TGGACAG856.4048124E-427.9239545
TCCGGCT458.981844E-426.36404666-67
GTATCAA20200.025.615392
GAGTACT14200.021.55539312-13
TATCAAC24050.021.5147953
ATCAACG24450.021.356974
CAACGCA24900.020.9641826
TCAACGC25000.020.8871175
ATGGGAG2056.7904875E-720.8408055
AACGCAG25400.020.738337
Produced by FastQC (version 0.11.5)