##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938844_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 153770 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.726825778760485 31.0 31.0 34.0 30.0 34.0 2 31.92183780971581 31.0 31.0 34.0 30.0 34.0 3 31.944579566885608 33.0 31.0 34.0 30.0 34.0 4 35.68314365610978 37.0 35.0 37.0 33.0 37.0 5 35.58616765298823 37.0 35.0 37.0 33.0 37.0 6 35.63617740781687 37.0 35.0 37.0 33.0 37.0 7 35.532320998894455 37.0 35.0 37.0 33.0 37.0 8 35.54948949730117 37.0 35.0 37.0 33.0 37.0 9 37.07419522663719 39.0 37.0 39.0 33.0 39.0 10-11 37.02077453339403 39.0 37.0 39.0 33.0 39.0 12-13 36.92145086817975 39.0 37.0 39.0 32.5 39.0 14-15 37.94507055992716 40.0 37.0 41.0 33.0 41.0 16-17 37.85286466801067 40.0 37.0 41.0 32.5 41.0 18-19 38.06303895428237 40.0 37.0 41.0 33.0 41.0 20-21 38.104877414320086 40.0 37.0 41.0 33.0 41.0 22-23 37.984831241464526 40.0 37.0 41.0 33.0 41.0 24-25 37.85321259023216 40.0 37.0 41.0 33.0 41.0 26-27 37.55110879885544 40.0 36.5 41.0 32.0 41.0 28-29 37.24561032711192 39.0 36.0 41.0 31.5 41.0 30-31 36.83637575599922 39.0 35.0 41.0 31.0 41.0 32-33 36.38096182610392 38.0 35.0 40.5 30.0 41.0 34-35 36.08170319308058 38.0 35.0 41.0 30.0 41.0 36-37 35.746897964492426 38.0 35.0 41.0 29.0 41.0 38-39 35.3843012291084 38.0 35.0 40.5 27.0 41.0 40-41 35.03479547375951 38.0 35.0 40.0 25.5 41.0 42-43 34.573642453014244 38.0 34.0 40.0 23.5 41.0 44-45 34.02089484294726 38.0 33.5 40.0 21.0 41.0 46-47 33.27525525134942 38.0 33.0 40.0 11.0 41.0 48-49 32.604679066137734 38.0 32.5 40.0 2.0 41.0 50-51 32.09593873967614 37.5 31.5 40.0 2.0 41.0 52-53 31.77037458541978 37.0 31.0 40.0 2.0 41.0 54-55 31.39022891331209 37.0 31.0 40.0 2.0 41.0 56-57 30.973232750211356 36.0 30.5 40.0 2.0 41.0 58-59 30.5194641347467 36.0 29.5 40.0 2.0 41.0 60-61 30.038603108538727 35.0 28.5 39.0 2.0 41.0 62-63 29.562456916173506 35.0 28.0 39.0 2.0 40.0 64-65 29.029774988619366 35.0 26.5 38.5 2.0 40.0 66-67 28.496120829810756 35.0 26.0 38.0 2.0 40.0 68-69 28.092248162840605 34.5 25.5 37.5 2.0 40.0 70-71 27.639959029719712 34.0 24.5 37.0 2.0 39.5 72-73 27.12541132860766 34.0 22.5 36.5 2.0 39.0 74-75 26.623831046367954 33.5 21.0 36.0 2.0 39.0 76-77 24.868156987708915 31.0 18.0 34.5 2.0 36.0 78-79 25.675814528191452 33.0 18.0 35.0 2.0 37.0 80-81 25.657244586070107 33.0 17.5 35.0 2.0 37.0 82-83 25.294849450477987 33.0 12.0 35.0 2.0 36.0 84-85 24.8456298367692 33.0 4.5 35.0 2.0 36.0 86-87 24.209306106522728 32.0 2.0 35.0 2.0 35.5 88-89 23.594433244456006 31.5 2.0 34.5 2.0 35.0 90-91 23.009618261039215 31.0 2.0 34.0 2.0 35.0 92-93 22.660255576510373 31.0 2.0 34.0 2.0 35.0 94-95 22.228184951551015 31.0 2.0 34.0 2.0 35.0 96-97 21.57354490472784 30.0 2.0 34.0 2.0 35.0 98-99 20.714749300903947 29.5 2.0 34.0 2.0 35.0 100-101 17.773548156337384 23.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20286979827576346 1101 2 -0.1612372492169314 1101 3 -0.11844778813715351 1101 4 0.24962801126362422 1101 5 -0.47430705056751066 1101 6 -0.09550452481020955 1101 7 -0.33197112647041394 1101 8 -0.32174530082384223 1101 9 -0.14936612751205303 1101 10-11 0.07968359870758945 1101 12-13 -0.11627798760935093 1101 14-15 0.06009880410989865 1101 16-17 0.28576274143431846 1101 18-19 0.17940567267854846 1101 20-21 -0.21117729400743457 1101 22-23 -0.15380212469363386 1101 24-25 -0.5379866388203567 1101 26-27 -0.6501269201768451 1101 28-29 -0.5533135485011442 1101 30-31 -0.08161207829846262 1101 32-33 0.012220286405522529 1101 34-35 -0.17845538621345725 1101 36-37 -0.3195749386033029 1101 38-39 -0.5478918822287042 1101 40-41 -0.6889653600170362 1101 42-43 -0.8957597756806095 1101 44-45 -0.5145510821666122 1101 46-47 -0.8889057901466515 1101 48-49 -0.4105490636766973 1101 50-51 -0.2499474530962189 1101 52-53 0.04535230907414345 1101 54-55 0.13642637807593516 1101 56-57 0.11235713573184825 1101 58-59 0.02610592598725958 1101 60-61 -0.44133485375958514 1101 62-63 -0.4965351724632221 1101 64-65 -0.051844203172759507 1101 66-67 -0.18589529728686216 1101 68-69 0.04081001495330483 1101 70-71 0.16742718822424152 1101 72-73 0.6662870605525981 1101 74-75 -0.21447506520271986 1101 76-77 0.055564484115940616 1101 78-79 0.43052789350356235 1101 80-81 1.0174856028153094 1101 82-83 0.8151216453001489 1101 84-85 1.011066106443728 1101 86-87 1.6059441513513164 1101 88-89 0.4108569233366346 1101 90-91 0.14009612438803032 1101 92-93 -0.3537812569432397 1101 94-95 -0.7295485184708248 1101 96-97 -1.1361577992882133 1101 98-99 -1.9563209558024077 1101 100-101 -0.9549269252714048 1102 1 0.18938707532765875 1102 2 0.14693335216264458 1102 3 0.2636079779174487 1102 4 0.40225601087500706 1102 5 0.38457762834048737 1102 6 0.4195542529999514 1102 7 0.009571669604589772 1102 8 0.06725693370448482 1102 9 0.10575871461366404 1102 10-11 0.24984778796242324 1102 12-13 0.2872972577029955 1102 14-15 0.5289125610320795 1102 16-17 0.5729724956362006 1102 18-19 0.5510417360225759 1102 20-21 0.5098224145323513 1102 22-23 0.305077696544096 1102 24-25 0.49329675766269077 1102 26-27 0.5898419907334826 1102 28-29 0.7491007136187449 1102 30-31 0.44061176314717443 1102 32-33 0.48958354298766693 1102 34-35 0.9548633741090953 1102 36-37 1.0460133252653492 1102 38-39 1.0961623293714098 1102 40-41 0.8952909306659649 1102 42-43 0.6118910549810082 1102 44-45 1.1403114457649934 1102 46-47 0.8510631207636763 1102 48-49 0.4499629347688483 1102 50-51 -0.0036392824948379143 1102 52-53 -0.46152129434565836 1102 54-55 -0.46959227537786674 1102 56-57 -0.47823841464219186 1102 58-59 -0.9752056449832978 1102 60-61 -0.45768091418899814 1102 62-63 0.1420784484441917 1102 64-65 0.16211188348808037 1102 66-67 0.38478380501567244 1102 68-69 0.412810403566926 1102 70-71 0.21095191376587152 1102 72-73 0.22263603558417344 1102 74-75 -0.008266962608725947 1102 76-77 0.011165377927270015 1102 78-79 -0.4186171565304413 1102 80-81 0.17961034778761586 1102 82-83 0.5827792767001334 1102 84-85 0.6195378833795075 1102 86-87 0.46954077041281295 1102 88-89 0.34348103681181286 1102 90-91 0.24288442711803881 1102 92-93 -0.07140488465139327 1102 94-95 -0.10111171676092212 1102 96-97 -0.03438960731308427 1102 98-99 0.38148143417135927 1102 100-101 0.4943104204975661 1103 1 0.29738852683813377 1103 2 0.3022449733184125 1103 3 0.3157262760214117 1103 4 0.14742520256510971 1103 5 0.054132196062525395 1103 6 0.026330992544544074 1103 7 0.467568585144825 1103 8 0.38413981495278193 1103 9 0.06584217646615542 1103 10-11 0.40543156733283325 1103 12-13 -0.08750996356162943 1103 14-15 0.44681149961504474 1103 16-17 0.24513524624855876 1103 18-19 0.348397265370302 1103 20-21 0.5373784337648573 1103 22-23 0.6574091852495343 1103 24-25 0.35363423384884385 1103 26-27 0.11524342410007904 1103 28-29 0.25665310407506325 1103 30-31 0.43487376078847007 1103 32-33 0.3889757229749691 1103 34-35 0.49988287878114335 1103 36-37 -0.003338030989752383 1103 38-39 0.33001427530264493 1103 40-41 0.18588786435007165 1103 42-43 0.10247891672464249 1103 44-45 0.20544797846933704 1103 46-47 0.6711665408852383 1103 48-49 0.5481893704034277 1103 50-51 0.4184100688614123 1103 52-53 0.969146400474262 1103 54-55 1.1333969290129495 1103 56-57 0.9735895813661557 1103 58-59 1.0864835503355792 1103 60-61 1.7489098505755827 1103 62-63 1.88409514081469 1103 64-65 1.858587434608463 1103 66-67 1.7024378012054555 1103 68-69 1.1575259982326251 1103 70-71 1.3840445382782534 1103 72-73 1.4871738202163058 1103 74-75 1.5419008682903055 1103 76-77 1.747852305261027 1103 78-79 1.5202606444311826 1103 80-81 1.7614619308412323 1103 82-83 2.090989382841837 1103 84-85 1.8897002711142434 1103 86-87 1.401773374966929 1103 88-89 1.0289115003879736 1103 90-91 1.2362165508593073 1103 92-93 1.4439266947734488 1103 94-95 2.0552431775509667 1103 96-97 2.246069432875615 1103 98-99 1.9750585003058063 1103 100-101 2.344260524824879 1104 1 -0.24230242238923694 1104 2 -0.22932235559990843 1104 3 0.044247247323841776 1104 4 0.06636559991610369 1104 5 0.16512557354596424 1104 6 0.024211787246528615 1104 7 -0.27892148108033865 1104 8 0.15711994740311042 1104 9 0.033612595892201114 1104 10-11 0.1518112582820592 1104 12-13 -0.34521415782212017 1104 14-15 -0.20720616042910223 1104 16-17 0.06895423079602381 1104 18-19 0.2178453889906038 1104 20-21 -0.09465247131240062 1104 22-23 -0.16117801121846043 1104 24-25 -0.387844794848732 1104 26-27 0.08363194507138161 1104 28-29 -0.3194837612671009 1104 30-31 -0.31267590808569423 1104 32-33 -0.5487726213958979 1104 34-35 -0.22888091812835398 1104 36-37 0.22560236636122966 1104 38-39 -0.5548422368386312 1104 40-41 -0.17534833874043443 1104 42-43 -0.7723555203614509 1104 44-45 -1.129231933230436 1104 46-47 -1.1118135915650953 1104 48-49 -0.42026537573343603 1104 50-51 -1.6577488715359365 1104 52-53 -1.9686902441173473 1104 54-55 -1.8393183027751299 1104 56-57 -2.1530329351901365 1104 58-59 -1.7121919463531654 1104 60-61 -1.9653064849652537 1104 62-63 -1.9723507753000966 1104 64-65 -1.4509931393429696 1104 66-67 -1.8420655100528194 1104 68-69 -1.8367786375289654 1104 70-71 -2.0016508259601586 1104 72-73 -2.3383228685254167 1104 74-75 -2.7365318027913723 1104 76-77 -2.157556083259948 1104 78-79 -2.69784090082268 1104 80-81 -3.0451385106598714 1104 82-83 -2.689275517820416 1104 84-85 -2.391558007031449 1104 86-87 -2.093701238719607 1104 88-89 -1.8865189631881876 1104 90-91 -1.7984854358956603 1104 92-93 -1.4717245193545914 1104 94-95 -1.115860575208412 1104 96-97 -1.034526593614455 1104 98-99 -0.37582450190170036 1104 100-101 0.21947023784916198 1105 1 0.06978091094409677 1105 2 0.06859431106675729 1105 3 -0.03741941934282522 1105 4 0.3063655999161057 1105 5 -0.03862442645403519 1105 6 -0.06828821275347252 1105 7 0.016078518919663054 1105 8 0.2683699474031087 1105 9 0.3815292625588711 1105 10-11 -0.11089707505127677 1105 12-13 -0.010005824488786175 1105 14-15 0.1182105062375598 1105 16-17 0.2285375641293541 1105 18-19 0.4186787223239392 1105 20-21 0.17638919535426822 1105 22-23 0.18611365544820302 1105 24-25 0.08798853848460908 1105 26-27 0.2038402784047122 1105 28-29 0.017807905399571666 1105 30-31 -0.4483009080856988 1105 32-33 -0.5175226213958908 1105 34-35 -0.34179758479501743 1105 36-37 -0.21939763363876352 1105 38-39 -0.5415089035052958 1105 40-41 -0.3130566720737704 1105 42-43 0.06514447963854053 1105 44-45 -0.023815266563779858 1105 46-47 -0.3849385915650885 1105 48-49 -0.1398487090667686 1105 50-51 -0.47024887153593653 1105 52-53 -0.27410691078401683 1105 54-55 -0.04056830277512802 1105 56-57 -0.31115793519013835 1105 58-59 0.050308053646837436 1105 60-61 0.028235181701411705 1105 62-63 0.21348255803323113 1105 64-65 0.1831735273236994 1105 66-67 0.15918448994717949 1105 68-69 -0.5686536375289677 1105 70-71 -0.9116508259601552 1105 72-73 -0.8851978685254167 1105 74-75 -0.7306984694580407 1105 76-77 -0.5096394165932807 1105 78-79 -0.9207575674893462 1105 80-81 -1.0578468439932074 1105 82-83 -0.8411505178204131 1105 84-85 -0.5434330070314495 1105 86-87 -1.1707845720529413 1105 88-89 -1.0440189631881864 1105 90-91 -0.7741104358956612 1105 92-93 -0.3271411860212545 1105 94-95 -0.6854439085417461 1105 96-97 -0.5426515936144511 1105 98-99 0.25438383143163534 1105 100-101 1.4034285711824985 1106 1 0.2942407874873041 1106 2 0.29043073081984616 1106 3 0.44498798806457884 1106 4 0.34340263695314377 1106 5 0.15203915379287736 1106 6 0.1898907995922059 1106 7 -0.0342301230556501 1106 8 0.06674957703273776 1106 9 0.3570693860156595 1106 10-11 0.2787248385289729 1106 12-13 0.3675018915605932 1106 14-15 0.10501606179311551 1106 16-17 0.07352213203058966 1106 18-19 0.07006761121282779 1106 20-21 0.2957178990579763 1106 22-23 0.5689454455716643 1106 24-25 0.46746384712658084 1106 26-27 0.18103935247879122 1106 28-29 0.7296520412020371 1106 30-31 0.6228796474698584 1106 32-33 1.1222150329250908 1106 34-35 1.3792672300197921 1106 36-37 1.1617752058674071 1106 38-39 1.0708367755070398 1106 40-41 1.6521825254570928 1106 42-43 1.0004839858113783 1106 44-45 0.5679285605967195 1106 46-47 0.6613962849781245 1106 48-49 0.3225741304394063 1106 50-51 0.4008931037727095 1106 52-53 0.18785296575919475 1106 54-55 0.5592002157433917 1106 56-57 0.9175843487604816 1106 58-59 0.4248450906838741 1106 60-61 0.7671626508372142 1106 62-63 0.8478961382801451 1106 64-65 0.6444389594224624 1106 66-67 0.2668233788360652 1106 68-69 -0.19369221777587597 1106 70-71 0.3525467049040394 1106 72-73 0.1666154030795184 1106 74-75 0.2049496786901095 1106 76-77 0.36466613896227784 1106 78-79 0.7941344078192962 1106 80-81 0.5416516127969189 1106 82-83 0.08442818588328649 1106 84-85 0.5198000176599074 1106 86-87 0.8618543168359452 1106 88-89 1.1768143701451486 1106 90-91 1.0001565394129805 1106 92-93 1.2854359744725734 1106 94-95 1.9183369556557857 1106 96-97 2.0978190853978944 1106 98-99 1.8173853746415105 1106 100-101 1.602927027972619 1107 1 -0.15281524290205795 1107 2 -0.12291209918965151 1107 3 -0.22523993216334048 1107 4 0.024314317864821078 1107 5 0.08461275303314864 1107 6 0.1134425564772954 1107 7 0.10774518558632451 1107 8 0.25122251150567365 1107 9 0.33617669845630616 1107 10-11 -0.33677848530768273 1107 12-13 -0.0023936450016037725 1107 14-15 0.19689640367345618 1107 16-17 0.23639012823192473 1107 18-19 -0.05600076485554695 1107 20-21 0.2554757338158069 1107 22-23 0.2083091682687197 1107 24-25 -0.06874223074616737 1107 26-27 0.20311912455856174 1107 28-29 0.3630803412969996 1107 30-31 0.21796511755532322 1107 32-33 0.019176096552818933 1107 34-35 0.14778574853831827 1107 36-37 0.19726903302790078 1107 38-39 0.09374750675110732 1107 40-41 0.005933712541619229 1107 42-43 0.33084960784366757 1107 44-45 0.5516655026669994 1107 46-47 0.022032562281061985 1107 48-49 -1.0073166577847203 1107 50-51 -0.6256975894846555 1107 52-53 -0.8906133210404228 1107 54-55 -0.3823952258520471 1107 56-57 -0.1195713967285954 1107 58-59 -0.5256534848147041 1107 60-61 -0.003255202913976518 1107 62-63 -0.886196929146255 1107 64-65 -0.8229162162660479 1107 66-67 -0.3111680741553826 1107 68-69 -0.39549658624691375 1107 70-71 -0.6879328772422078 1107 72-73 -0.5537074839100313 1107 74-75 -0.8951215463811195 1107 76-77 -1.287043262747126 1107 78-79 -1.0625844905662696 1107 80-81 -0.9119333824547446 1107 82-83 -0.6192755178204159 1107 84-85 -0.37989134036478234 1107 86-87 -0.5110089310272983 1107 88-89 -0.3135702452394682 1107 90-91 -0.016818769228994057 1107 92-93 -0.22121169884176695 1107 94-95 -0.2727836521314906 1107 96-97 0.2553452012573416 1107 98-99 -0.0014655275427237768 1107 100-101 -0.1487348903559642 1108 1 0.024080556334165948 1108 2 -0.20379044070629249 1108 3 -0.2035541711158757 1108 4 -0.19008830079311423 1108 5 -0.10551272432637404 1108 6 -0.21607190069673265 1108 7 -0.027006587463318965 1108 8 0.20307739421162552 1108 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-1.0667726889114313 2316 94-95 -0.8890204981371213 2316 96-97 -0.7454514491057829 2316 98-99 -0.8520287408226999 2316 100-101 -0.8244796658117259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 20.0 6 50.0 7 103.0 8 209.0 9 418.0 10 713.0 11 1046.0 12 1395.0 13 1745.0 14 1983.0 15 2646.0 16 3184.0 17 3988.0 18 3701.0 19 2269.0 20 1993.0 21 2050.0 22 2173.0 23 2211.0 24 2512.0 25 2387.0 26 2501.0 27 2858.0 28 3275.0 29 3780.0 30 4433.0 31 5321.0 32 6255.0 33 7437.0 34 9641.0 35 13078.0 36 17281.0 37 23711.0 38 16356.0 39 1040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.783370587353616 21.86430847632143 16.431303803820114 19.92101713250484 2 16.313975417831827 23.029199453729596 38.988749430968326 21.66807569747025 3 18.915913377121676 28.13617740781687 33.20153475970605 19.7463744553554 4 12.76451843662613 20.82200689341224 35.36775703973467 31.04571763022696 5 11.77561096186245 39.4469632414641 34.04604086305478 14.731384933618678 6 26.87407734979092 37.08419772515917 18.34895199942771 17.6927729256222 7 22.9557130779736 37.46569551928204 21.225206477206218 18.35338492553814 8 24.88716914872862 34.07621772777525 21.16602718345581 19.87058594004032 9 25.07121024907329 17.18345581062626 22.012746309423164 35.73258763087728 10-11 23.124146452493985 27.91311699291149 27.17207517721272 21.790661377381802 12-13 25.77778500357677 26.393639851726604 27.86499317161995 19.96358197307667 14-15 22.846458997203616 27.467971645964752 28.039279443324443 21.646289913507186 16-17 19.398233656772717 30.76727972737442 29.1550688067428 20.679417809110063 18-19 18.75398322169474 27.628275996618324 33.54913182025103 20.06860896143591 20-21 20.161930155426937 26.60044221889836 33.597580802497234 19.640046823177475 22-23 19.999024517135982 27.475775508876893 33.277947584054104 19.247252389933017 24-25 19.734993821941863 27.933927294010534 33.856083761461925 18.474995122585682 26-27 19.729269228518103 27.933458629502695 34.23284277064948 18.104429371329722 28-29 18.75138193405736 27.306366651492485 34.957729075892566 18.984522338557586 30-31 19.150354425440593 28.609936918774793 33.78617415620732 18.45353449957729 32-33 19.896598816414123 27.83507836379008 33.55986213175522 18.708460688040578 34-35 19.280599402938478 27.559462254395033 34.005840536444815 19.154097806221667 36-37 19.129145146812025 28.330427881925925 33.61227835091782 18.92814862034423 38-39 19.275674339644734 26.99635105338129 34.05911163435083 19.668862972623145 40-41 18.822580592709663 27.397131084899982 34.37982718867784 19.400461133712515 42-43 19.399170102891635 27.548551582397856 33.55815133264826 19.494126982062255 44-45 18.709759238303352 28.253079434450644 32.79939126702306 20.237770060222942 46-47 19.856604018989398 27.50113806334135 31.597190609351628 21.045067308317616 48-49 19.790267774399663 27.641081502918368 31.1857186986847 21.38293202399727 50-51 19.532743708135527 28.390453274370813 31.13025947844183 20.94654353905183 52-53 19.40105352149314 28.717240033816736 31.482408792352214 20.399297652337907 54-55 18.774793522793782 28.831371528906807 30.846719125967354 21.547115822332056 56-57 19.664492381330195 29.412032490716474 29.548602756118026 21.37487237183531 58-59 18.890612247552344 29.569257882740075 30.346395091354267 21.193734778353317 60-61 19.28866170459756 30.055961474053188 29.613732510007058 21.041644311342196 62-63 18.88384524839207 30.30910001365685 29.66787844103818 21.1391762969129 64-65 18.860292324450874 30.275578389451606 29.47566943599915 21.388459850098364 66-67 18.615276057061656 30.076058362355173 29.324904805106545 21.983760775476625 68-69 18.70065682512844 29.890745919230017 29.307732327502112 22.10086492813943 70-71 18.897379202705338 29.4228393054562 28.961761071730507 22.718020420107955 72-73 18.84015087468297 29.192300188593357 28.73284775964102 23.234701177082655 74-75 18.68972289603236 29.033160129805097 28.736936573691708 23.540180400470838 76-77 18.902133529400285 28.7694630064611 29.15302981996109 23.175373644177526 78-79 18.652119270416172 28.693664654515615 29.212330774189766 23.441885300878443 80-81 18.51315934941373 28.952194525554564 28.805220818240347 23.729425306791356 82-83 18.64192392484831 28.621503684097576 28.698242168447475 24.03833022260664 84-85 18.661962671522403 28.34525590167133 28.424920335566107 24.567861091240164 86-87 18.827328270613545 28.31292946587547 28.29632860588582 24.563413657625166 88-89 18.88433580852702 27.878554556487924 28.374137053518993 24.862972581466064 90-91 18.529893663548595 27.91935662856658 28.656335472247424 24.8944142356374 92-93 18.51700109579598 28.068310035475175 28.28909504160448 25.125593827124366 94-95 18.638936850285656 27.699901475910373 28.198700002276134 25.462461671527837 96-97 18.776744488521818 27.30669181244716 27.947909215061458 25.968654483969566 98-99 18.672367822071926 27.371398842427002 27.930350523509134 26.025882811991934 100-101 18.76678654345154 26.991136054731445 28.71027320201081 25.531804199806203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 564.0 1 381.5 2 201.5 3 194.0 4 173.5 5 182.0 6 279.5 7 454.5 8 584.5 9 656.0 10 618.5 11 513.5 12 478.0 13 509.5 14 523.0 15 515.5 16 569.5 17 629.0 18 669.5 19 731.0 20 816.5 21 869.5 22 949.0 23 1091.0 24 1220.5 25 1387.5 26 1587.5 27 1845.5 28 2050.0 29 2245.5 30 2515.0 31 2747.5 32 3023.5 33 3291.5 34 3520.5 35 3731.5 36 3986.5 37 4175.5 38 4196.5 39 4160.0 40 4144.0 41 4271.5 42 4505.0 43 4727.5 44 5065.0 45 5455.5 46 5674.0 47 5769.0 48 5870.0 49 5719.5 50 5329.0 51 4903.0 52 4360.0 53 3948.0 54 3664.0 55 3301.5 56 2925.5 57 2607.5 58 2384.5 59 2143.5 60 1869.5 61 1548.0 62 1215.5 63 942.5 64 675.0 65 497.0 66 386.5 67 298.5 68 223.5 69 160.5 70 133.5 71 120.5 72 96.5 73 74.5 74 50.0 75 36.0 76 31.5 77 23.0 78 19.5 79 13.5 80 8.5 81 6.0 82 3.5 83 2.5 84 3.0 85 3.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20745268908109515 2 0.0 3 0.0 4 0.0 5 0.024061910645769655 6 6.503219093451258E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0039019314560707554 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.503219093451258E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011055472458867139 36-37 0.02341158873642453 38-39 0.017558691552318397 40-41 0.012030955322884828 42-43 0.010405150549522013 44-45 0.0052025752747610066 46-47 0.0 48-49 0.0016258047733628146 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0019509657280353777 58-59 3.251609546725629E-4 60-61 0.0022761266827079404 62-63 6.503219093451258E-4 64-65 0.0016258047733628146 66-67 0.004227092410743318 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.503219093451258E-4 76-77 0.052676074656955195 78-79 0.13201534759706055 80-81 6.503219093451258E-4 82-83 6.503219093451258E-4 84-85 0.0 86-87 0.10632763217792807 88-89 0.33556610522208496 90-91 0.3752357416921376 92-93 3.251609546725629E-4 94-95 3.251609546725629E-4 96-97 0.0 98-99 0.0 100-101 6.503219093451258E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 153770.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.41824225060677 #Duplication Level Percentage of deduplicated Percentage of total 1 89.07200262470293 65.39509866447122 2 7.212798349694821 10.591019530853423 3 1.6378029649086412 3.607338445092739 4 0.6731340651237687 1.9768127944157023 5 0.4182166010736633 1.5352363865425793 6 0.22855007612861733 1.0067846913363268 7 0.1656712891577068 0.8514306388945587 8 0.10479181668065439 0.6154904786353168 9 0.08083936835080793 0.5341575896069086 >10 0.35729002029676377 4.518723728808772 >50 0.024950420347763822 1.3071839014153284 >100 0.016966285836479397 2.3791626277441544 >500 0.0029940504417316585 1.5980762830643673 >1k 0.003992067255642212 4.083484239118624 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1706 1.1094491773427848 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1574 1.023606685309228 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1258 0.8181049619561682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.6730831761722053 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 921 0.5989464785068609 RNA PCR Primer, Index 46 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 719 0.4675814528191454 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.35182415295571307 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.29264485920530664 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 400 0.26012876373805033 RNA PCR Primer, Index 46 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 328 0.21330558626520127 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 303 0.19704753853157314 RNA PCR Primer, Index 46 (96% over 28bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.11770826559146777 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168 0.10925408076998115 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 158 0.10275086167652989 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 157 0.10210053976718476 RNA PCR Primer, Index 46 (96% over 26bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.503219093451258E-4 0.0 20-21 0.0 0.0 0.0 0.0013006438186902516 0.0 22-23 0.0 0.0 0.0 0.00780386291214151 0.0 24-25 0.0 0.0 0.0 0.019834818235026336 0.0 26-27 0.0 0.0 0.0 0.030890290693893478 0.0 28-29 0.0 0.0 0.0 0.07121024907329128 0.0 30-31 0.0 0.0 0.0 0.1489237172400338 0.0 32-33 0.0 0.0 0.0 0.28158938674643946 0.0 34-35 0.0 0.0 0.0 0.4848149834167913 0.0 36-37 0.0 0.0 0.0 0.78786499317162 0.0 38-39 0.0 0.0 0.0 1.1130259478441829 0.0 40-41 0.0 0.0 0.0 1.429082395785914 0.0 42-43 0.0 0.0 0.0 1.7483904532743708 0.0 44-45 0.0 0.0 0.0 2.161019704753853 0.0 46-47 0.0 0.0 0.0 2.6006373154711584 0.0 48-49 0.0 0.0 0.0 3.0308252585029587 0.0 50-51 0.0 0.0 0.0 3.4519086948039277 0.0 52-53 0.0 0.0 0.0 3.869740521558171 0.0 54-55 0.0 0.0 0.0 4.371789035572608 0.0 56-57 0.0 0.0 0.0 4.938544579566885 0.0 58-59 0.0 0.0 0.0 5.5046498016518175 0.0 60-61 0.0 0.0 0.0 6.120504649801652 0.0 62-63 0.0 0.0 0.0 6.696689861481433 0.0 64-65 0.0 0.0 0.0 7.315145997268648 0.0 66-67 0.0 0.0 0.0 8.009689796449242 0.0 68-69 0.0 0.0 0.0 8.768615464655003 0.0 70-71 0.0 0.0 0.0 9.563308837874747 0.0 72-73 0.0 0.0 0.0 10.395070559927163 0.0 74-75 0.0 0.0 0.0 11.234636144891722 0.0 76-77 0.0 0.0 0.0 12.09468687000065 0.0 78-79 0.0 0.0 0.0 13.015217532678676 0.0 80-81 0.0 0.0 0.0 13.989399752877674 0.0 82-83 0.0 0.0 0.0 14.960980685439292 0.0 84-85 0.0 0.0 0.0 15.944592573323796 0.0 86-87 0.0 0.0 0.0 17.003966963647002 0.0 88-89 0.0 0.0 0.0 18.030825258502958 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGG 45 9.505437E-4 42.306297 1 GACAGCG 45 9.643637E-4 42.182476 7 CTTGGAC 60 8.287304E-5 39.558937 3 CTCCCGC 50 0.001618938 37.96423 7 GTATCCT 55 0.0025804986 34.524162 3 GGTATCA 1585 0.0 32.730663 1 GTTGCAC 60 0.0039523146 31.64715 3 ATACTGG 65 0.0057721096 29.288977 1 GTGTAAG 65 0.0057721096 29.288977 1 TCTAGGT 65 0.0058461274 29.212753 2 TGGACAG 85 6.4048124E-4 27.923954 5 TCCGGCT 45 8.981844E-4 26.364046 66-67 GTATCAA 2020 0.0 25.61539 2 GAGTACT 1420 0.0 21.555393 12-13 TATCAAC 2405 0.0 21.514795 3 ATCAACG 2445 0.0 21.35697 4 CAACGCA 2490 0.0 20.964182 6 TCAACGC 2500 0.0 20.887117 5 ATGGGAG 205 6.7904875E-7 20.840805 5 AACGCAG 2540 0.0 20.73833 7 >>END_MODULE