FastQCFastQC Report
Thu 26 May 2016
SRR938843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601358
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.42054815933271034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.3088010802217647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.2254896417774437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA902.4420091E-531.6076161
CGTGCAC1057.0164606E-527.076453
GTATCAA34750.026.3321021
GGTATCA24850.024.993551
GTACATG18400.024.4787251
GCGCCGT701.7605396E-523.78299994-95
AGCACCG1201.7381177E-423.6918935
TACATGG19000.023.1931172
GAGTACT20800.022.75792312-13
ATCAACG39500.022.6722433
CAACGCA39600.022.6149885
TATCAAC40300.022.4573272
TCAACGC39900.022.4449524
AACGCAG39800.022.3580866
GTACTAA2205.740185E-821.5506461
AGTACTT21650.021.42713712-13
ACATGGG19400.021.2494283
CGCAGAG42300.020.8146238
GACTGTA2057.0477836E-720.781877
CATGGGG8450.020.747934
Produced by FastQC (version 0.11.5)