##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601358 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.510532827367392 33.0 31.0 34.0 30.0 34.0 2 31.587282450719872 33.0 31.0 34.0 30.0 34.0 3 31.59677430083245 33.0 31.0 34.0 28.0 34.0 4 35.14020433751609 37.0 35.0 37.0 33.0 37.0 5 35.17445182403826 37.0 35.0 37.0 33.0 37.0 6 35.25254507298481 37.0 35.0 37.0 33.0 37.0 7 35.17578547221456 37.0 35.0 37.0 32.0 37.0 8 35.15058916651978 37.0 35.0 37.0 32.0 37.0 9 36.729971165262626 39.0 37.0 39.0 33.0 39.0 10-11 36.62388128203167 39.0 37.0 39.0 32.0 39.0 12-13 36.53183211996847 39.0 37.0 39.0 32.0 39.0 14-15 37.701446559287476 40.0 38.0 41.0 32.0 41.0 16-17 37.63643536795054 40.0 38.0 41.0 32.0 41.0 18-19 37.5968125476006 40.0 37.5 41.0 32.0 41.0 20-21 37.50534373867147 40.0 37.0 41.0 32.0 41.0 22-23 37.40695226470754 40.0 37.0 41.0 32.0 41.0 24-25 37.40810548791236 40.0 37.0 41.0 32.0 41.0 26-27 37.31577945250583 40.0 37.0 41.0 32.0 41.0 28-29 37.19713714625897 40.0 37.0 41.0 31.0 41.0 30-31 36.98944721779705 40.0 36.5 41.0 31.0 41.0 32-33 36.78315495927551 39.5 36.0 41.0 30.5 41.0 34-35 36.47033879984967 39.0 35.5 41.0 30.0 41.0 36-37 36.2095581999408 39.0 35.0 41.0 30.0 41.0 38-39 36.03926197040698 39.0 35.0 41.0 29.0 41.0 40-41 35.86178116862169 39.0 35.0 41.0 28.5 41.0 42-43 35.61558672205242 39.0 35.0 41.0 27.0 41.0 44-45 35.56519660501731 39.0 35.0 41.0 27.0 41.0 46-47 35.367448341919456 39.0 35.0 41.0 25.5 41.0 48-49 35.14493197063979 39.0 35.0 41.0 24.5 41.0 50-51 34.02354088579515 38.0 34.0 40.0 23.0 40.5 52-53 34.12572460996611 38.0 33.5 39.5 23.0 40.5 54-55 34.67268332673715 39.0 34.5 40.5 23.0 41.0 56-57 34.611083580828726 39.0 34.5 41.0 23.0 41.0 58-59 34.432984179141215 38.5 34.0 40.5 22.5 41.0 60-61 34.18360693630083 38.0 34.0 40.0 22.0 41.0 62-63 33.855814672790586 38.0 34.0 40.0 20.5 41.0 64-65 33.49323032203779 37.5 33.0 40.0 19.0 41.0 66-67 33.12385966429315 37.0 33.0 40.0 17.0 41.0 68-69 32.733129849440765 36.0 32.5 39.0 13.0 41.0 70-71 32.33355255937395 36.0 32.0 39.0 9.0 41.0 72-73 31.8940780699683 35.0 31.5 39.0 7.0 40.0 74-75 31.409748436039763 35.0 31.0 38.0 2.0 40.0 76-77 30.928768720130105 35.0 31.0 37.0 2.0 39.5 78-79 30.459965611166727 35.0 30.5 37.0 2.0 39.0 80-81 29.95661070443895 35.0 30.0 36.0 2.0 39.0 82-83 29.415118781158647 34.0 29.5 36.0 2.0 37.5 84-85 28.91815690487197 34.0 29.0 35.0 2.0 37.0 86-87 28.437227907502685 34.0 29.0 35.0 2.0 37.0 88-89 27.982569451142247 34.0 28.0 35.0 2.0 36.0 90-91 27.485898582874096 34.0 26.5 35.0 2.0 36.0 92-93 26.945179078020082 34.0 25.5 35.0 2.0 35.5 94-95 26.368424465958714 34.0 24.5 35.0 2.0 35.0 96-97 25.71223214790524 33.0 21.5 35.0 2.0 35.0 98-99 24.99177195613927 33.0 10.0 35.0 2.0 35.0 100-101 22.887493805686464 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4452183579513509 1101 2 0.21482394569356345 1101 3 0.4779233242637879 1101 4 0.3817069117762557 1101 5 0.038849080465489294 1101 6 -0.02407789702012053 1101 7 0.338444478603833 1101 8 0.14456644595910007 1101 9 0.4502886344803514 1101 10-11 0.5827886564379767 1101 12-13 0.42099670987126814 1101 14-15 0.7393681078897671 1101 16-17 0.7164953612203462 1101 18-19 0.624609632010781 1101 20-21 0.7262746408523952 1101 22-23 0.6027742700394896 1101 24-25 0.7250192413907044 1101 26-27 0.5832203150230626 1101 28-29 0.5663740600323806 1101 30-31 0.5585118403913967 1101 32-33 0.7210009998353399 1101 34-35 0.527130898443076 1101 36-37 0.7402127178465818 1101 38-39 0.13966115915437882 1101 40-41 0.20555475558432335 1101 42-43 0.4091306918185751 1101 44-45 0.4218895087490324 1101 46-47 0.5461667458474082 1101 48-49 0.4797186128040991 1101 50-51 0.49318681575561385 1101 52-53 0.3854810495600134 1101 54-55 0.06505691598946584 1101 56-57 0.15014895258168792 1101 58-59 -0.010217343682938917 1101 60-61 -0.1325872401124002 1101 62-63 -0.04025993731087851 1101 64-65 -0.12347737498489408 1101 66-67 -0.32544476364281394 1101 68-69 -0.1617667043190636 1101 70-71 -0.10958131157390483 1101 72-73 -0.3021595841179696 1101 74-75 -0.3632967652194736 1101 76-77 -0.7253910298506874 1101 78-79 -0.9265652807538345 1101 80-81 -0.6444683359761001 1101 82-83 -0.4910799562163497 1101 84-85 -0.4494499415656108 1101 86-87 -0.45626955529452573 1101 88-89 -0.6228071766301753 1101 90-91 -0.5426230230305329 1101 92-93 -0.4405188030959373 1101 94-95 -0.5797477028082696 1101 96-97 -0.6731133908293288 1101 98-99 -0.3930759900288443 1101 100-101 0.05026514734054999 1102 1 -0.02924495196277732 1102 2 -0.0048124061149188435 1102 3 -0.07754440927771711 1102 4 -0.14145533126303889 1102 5 -0.19608781779108142 1102 6 -0.09669369197198563 1102 7 0.07053204040450822 1102 8 -0.17040883377028138 1102 9 0.16981764827166046 1102 10-11 0.05201191951369566 1102 12-13 0.025425216246496518 1102 14-15 -0.07884520202436107 1102 16-17 0.04542783584954435 1102 18-19 0.08345851309326946 1102 20-21 0.041532902627054114 1102 22-23 0.133933195358253 1102 24-25 0.05826312637638864 1102 26-27 0.3833292793738323 1102 28-29 0.2629343644820281 1102 30-31 0.30922254817177475 1102 32-33 0.17949859286161995 1102 34-35 -0.07374569536385422 1102 36-37 0.15249966814582905 1102 38-39 0.21140361296650667 1102 40-41 0.28224776885520697 1102 42-43 0.4279327344935666 1102 44-45 0.5262984991211326 1102 46-47 0.3665788587800094 1102 48-49 0.4312473663820313 1102 50-51 0.29375603251335036 1102 52-53 0.13839056035886443 1102 54-55 0.009029723691774905 1102 56-57 -0.08711197638006496 1102 58-59 -0.14550065099493992 1102 60-61 -0.1973933811480535 1102 62-63 -0.09893317436526416 1102 64-65 -0.03970655791489719 1102 66-67 -0.16918825830843787 1102 68-69 -0.3902519372100386 1102 70-71 -0.47248301597151254 1102 72-73 -0.33955712510157454 1102 74-75 -0.46042466009327043 1102 76-77 -0.3586316621691914 1102 78-79 -0.6399418745607548 1102 80-81 -0.5031757260359484 1102 82-83 -0.3898276793493167 1102 84-85 -0.5550933191352172 1102 86-87 -0.5904843614355642 1102 88-89 -0.7009638901352524 1102 90-91 -0.5760327940427601 1102 92-93 -0.20050286502672066 1102 94-95 0.016007566512573845 1102 96-97 0.1575446237426199 1102 98-99 -0.04025918024482067 1102 100-101 0.17858612053857215 1103 1 0.06109174622275759 1103 2 0.1769375299954845 1103 3 0.3456059868377963 1103 4 0.29686245560126423 1103 5 0.2904921719865854 1103 6 0.3884040591141371 1103 7 0.2177240925384254 1103 8 0.18557123267490283 1103 9 0.34675350311449193 1103 10-11 0.44148930259573405 1103 12-13 0.4522700284110712 1103 14-15 0.40533094159945904 1103 16-17 0.471581043107399 1103 18-19 0.4898810150099493 1103 20-21 0.5506275870124355 1103 22-23 0.46771002898974245 1103 24-25 0.5484321143651485 1103 26-27 0.4213419934039919 1103 28-29 0.5053458773877324 1103 30-31 0.46816868924000943 1103 32-33 0.16970591460964357 1103 34-35 0.12382809712273968 1103 36-37 0.2837656405455178 1103 38-39 0.40511208472729976 1103 40-41 0.6582268769564052 1103 42-43 0.377059044230343 1103 44-45 0.37183101892691894 1103 46-47 0.13541830421829104 1103 48-49 0.021849206402471566 1103 50-51 0.2496817927995849 1103 52-53 0.374144777072388 1103 54-55 0.4056403804768536 1103 56-57 0.5489680772871992 1103 58-59 0.6859793515606469 1103 60-61 0.6443009710114183 1103 62-63 0.4999791052156226 1103 64-65 0.29482097850217315 1103 66-67 -0.21216838864322085 1103 68-69 0.05224902113393881 1103 70-71 0.07203365921376559 1103 72-73 0.37617409135638624 1103 74-75 0.28909936239587 1103 76-77 0.07089331866392712 1103 78-79 0.23363817910650653 1103 80-81 0.48957599898322 1103 82-83 0.5359996909547959 1103 84-85 0.48857202714640735 1103 86-87 0.11860393398994162 1103 88-89 0.05361539685682715 1103 90-91 0.291480942220975 1103 92-93 0.47354677719152605 1103 94-95 0.2422118854494535 1103 96-97 0.33805669887678746 1103 98-99 0.3157017193207281 1103 100-101 0.3818796293553355 1104 1 0.2251359180322794 1104 2 0.21531240851681588 1104 3 0.24470596145881274 1104 4 0.12955925104046884 1104 5 0.13960793109671243 1104 6 0.27345583842841137 1104 7 0.30920603941587643 1104 8 0.13258177423762874 1104 9 0.060252645800083826 1104 10-11 0.2995527005319829 1104 12-13 0.27211639271708776 1104 14-15 0.19402615239976484 1104 16-17 0.18218636278972866 1104 18-19 0.4261519139830341 1104 20-21 0.07092613050644303 1104 22-23 0.2948977282302252 1104 24-25 0.3643524738800963 1104 26-27 0.38508718841980993 1104 28-29 0.3310232176703707 1104 30-31 0.16767225158255172 1104 32-33 0.04374414402325755 1104 34-35 -0.08496974380675226 1104 36-37 0.1416031036376708 1104 38-39 0.3859803554281953 1104 40-41 0.19643153059519136 1104 42-43 0.3922771487298462 1104 44-45 0.7352116231942958 1104 46-47 0.6675087895758551 1104 48-49 0.710424331285644 1104 50-51 0.43904149964138384 1104 52-53 0.4737557358408253 1104 54-55 0.6254718887931077 1104 56-57 0.5664563627202739 1104 58-59 0.6272471245858782 1104 60-61 0.555844384546468 1104 62-63 0.3331509828270214 1104 64-65 0.10818951227788887 1104 66-67 0.015874149007423455 1104 68-69 0.23008605092639556 1104 70-71 0.2586125104743573 1104 72-73 0.3296686534451432 1104 74-75 0.021044692353591188 1104 76-77 -0.06344196864472096 1104 78-79 0.10060743339772316 1104 80-81 0.17490756610443725 1104 82-83 0.08651315110050817 1104 84-85 0.3136597703358639 1104 86-87 0.2160097067799569 1104 88-89 0.3144741968642535 1104 90-91 0.27704122968437517 1104 92-93 0.4815256816860831 1104 94-95 0.3221185403138591 1104 96-97 0.11634652685680535 1104 98-99 0.141092772300901 1104 100-101 0.49080188919897694 1105 1 -0.13637862018135394 1105 2 0.08651018638692065 1105 3 -0.20523676409271374 1105 4 0.04130318380679654 1105 5 -4.7706062371588587E-4 1105 6 -0.1085320894235906 1105 7 0.07147849960568209 1105 8 0.10431549850122224 1105 9 -0.22789124660704374 1105 10-11 -0.1735639197413903 1105 12-13 -0.105696138813542 1105 14-15 0.0025395688651244086 1105 16-17 -0.19394057875472015 1105 18-19 -0.3031180000162621 1105 20-21 -0.20448829315829187 1105 22-23 -0.19174219057656217 1105 24-25 -0.19062654527271405 1105 26-27 -0.23799055645071832 1105 28-29 -0.23052158994089922 1105 30-31 -0.24921747878240552 1105 32-33 0.03182185921790648 1105 34-35 -0.21560890291101487 1105 36-37 -0.15999880036680025 1105 38-39 0.019276989589883442 1105 40-41 -0.14564827378140421 1105 42-43 0.13562233263127865 1105 44-45 0.17636435307850462 1105 46-47 0.26285887593267176 1105 48-49 -0.014495098700059827 1105 50-51 -0.17766334999092948 1105 52-53 -0.2382680404742672 1105 54-55 -0.05396035226999629 1105 56-57 -0.4165823880438779 1105 58-59 -0.5337311704754626 1105 60-61 -0.2388415556150889 1105 62-63 -0.06499694549979296 1105 64-65 0.0074280291961059675 1105 66-67 -0.313106721920299 1105 68-69 -0.3597799327993485 1105 70-71 -0.3427246846801566 1105 72-73 -0.22318705649093928 1105 74-75 0.028202514618811847 1105 76-77 -0.01626918973596503 1105 78-79 -0.08354178879746499 1105 80-81 -0.02771934279654431 1105 82-83 0.20646305821500732 1105 84-85 0.17491464459915917 1105 86-87 0.26929571697658616 1105 88-89 0.29882017014409 1105 90-91 0.4589172794686327 1105 92-93 0.32650185195441495 1105 94-95 0.4202804163042906 1105 96-97 0.15695500110111027 1105 98-99 0.30006794545966287 1105 100-101 0.6267085170100266 1106 1 -0.194125904343732 1106 2 -0.14015967973939425 1106 3 -0.09702689138973142 1106 4 -0.050562782533702944 1106 5 0.03540428682278929 1106 6 -0.16961806897666776 1106 7 -0.1476489366495244 1106 8 -0.25998445676559356 1106 9 -0.02402641785219828 1106 10-11 0.04054510480853679 1106 12-13 -0.12918402382258876 1106 14-15 -0.18581380552793547 1106 16-17 -0.1431269383917595 1106 18-19 0.019359388987666648 1106 20-21 0.1631311014920911 1106 22-23 0.12090426617000105 1106 24-25 0.0546623985204846 1106 26-27 0.0038248999018364316 1106 28-29 0.10291246712205293 1106 30-31 -0.049442329696468335 1106 32-33 0.1147456320918181 1106 34-35 0.09563038408347779 1106 36-37 0.17896617443746266 1106 38-39 0.03372195740271877 1106 40-41 0.05638575306443272 1106 42-43 -0.005004287465474988 1106 44-45 -0.166967291651126 1106 46-47 -0.20223590805937164 1106 48-49 0.1354232854543227 1106 50-51 0.051290574827689284 1106 52-53 -0.1154013838799699 1106 54-55 -0.2550493535349645 1106 56-57 -0.06950619054241258 1106 58-59 -0.20879789994633313 1106 60-61 -0.10609064243598709 1106 62-63 -0.14046568115036706 1106 64-65 -0.35407091764842846 1106 66-67 -0.32896034071652736 1106 68-69 -0.013399914017334424 1106 70-71 -0.1673217157001048 1106 72-73 -0.3659496121482064 1106 74-75 -0.4711235251315138 1106 76-77 -0.5416797129957409 1106 78-79 0.027944567580870938 1106 80-81 -0.20527602211292617 1106 82-83 -0.026636833062617882 1106 84-85 -0.008063341340989183 1106 86-87 -0.09778450947405304 1106 88-89 0.22582953448848286 1106 90-91 0.07100797822469573 1106 92-93 -0.02965873722015644 1106 94-95 0.043613660763742956 1106 96-97 -0.012090523062216363 1106 98-99 -0.11221205465883344 1106 100-101 0.08673748989460606 1107 1 0.09078400556617794 1107 2 -0.033769935072445634 1107 3 -0.1051023243356326 1107 4 0.14802343321572664 1107 5 -0.16220750891076818 1107 6 -0.24437708965538008 1107 7 -0.35678456191209307 1107 8 -0.07373894960039706 1107 9 -0.23901439806038383 1107 10-11 -0.13037225037047762 1107 12-13 -0.06139910807782911 1107 14-15 0.1442242960451381 1107 16-17 0.0825417354634439 1107 18-19 0.11456372544848392 1107 20-21 0.10034081768496605 1107 22-23 0.1565683304933856 1107 24-25 -0.19704575393249257 1107 26-27 -0.0651021878076321 1107 28-29 0.03445946100712405 1107 30-31 -0.004508146808923641 1107 32-33 -0.13271183184880186 1107 34-35 -0.3097254250935748 1107 36-37 -0.018253227607068823 1107 38-39 0.14289492570782159 1107 40-41 0.10739255649999535 1107 42-43 0.06058234839318288 1107 44-45 0.3280118195844537 1107 46-47 0.3032342641854129 1107 48-49 0.3751904165750801 1107 50-51 0.21419522170254623 1107 52-53 0.3171550391233424 1107 54-55 0.5063752835373379 1107 56-57 0.27234748307939327 1107 58-59 0.37390089340659927 1107 60-61 0.2177610590063921 1107 62-63 0.2459268642447725 1107 64-65 0.14774517953684096 1107 66-67 0.12275189613171733 1107 68-69 0.3066109971528945 1107 70-71 0.31583744734894736 1107 72-73 0.2060509830065378 1107 74-75 -0.08286674503535707 1107 76-77 0.004809457521925964 1107 78-79 0.17407839570951467 1107 80-81 0.12884647473624966 1107 82-83 -0.04134794961958832 1107 84-85 0.20658621754432005 1107 86-87 0.418516603815398 1107 88-89 0.2797552218338595 1107 90-91 -0.15725866276862988 1107 92-93 -0.010300162324018203 1107 94-95 0.09735409160909825 1107 96-97 0.04014597509397433 1107 98-99 0.030807422071781332 1107 100-101 0.004575502777957041 1108 1 0.4790660478041744 1108 2 0.5030091972409458 1108 3 0.43195512463046626 1108 4 0.4636978964228149 1108 5 0.6180959389089935 1108 6 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-3.2041991175647517 2316 98-99 -3.5036259595017256 2316 100-101 -3.751775989534945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4771.0 3 964.0 4 797.0 5 824.0 6 835.0 7 920.0 8 1072.0 9 1305.0 10 1462.0 11 2057.0 12 2735.0 13 3538.0 14 3913.0 15 3808.0 16 4639.0 17 5654.0 18 5044.0 19 3679.0 20 3783.0 21 4072.0 22 4520.0 23 5111.0 24 5924.0 25 6737.0 26 7857.0 27 9398.0 28 11087.0 29 12878.0 30 15146.0 31 18251.0 32 22225.0 33 27391.0 34 35853.0 35 48361.0 36 68541.0 37 104135.0 38 115293.0 39 26715.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.43994536702367 21.398470942918536 14.453253826806803 22.708329863250995 2 14.939854091869192 22.40021039000381 40.51755275146516 22.14238276666184 3 17.923914364130532 26.63296844494968 31.87194897665369 23.5711682142661 4 12.371070522509806 18.314311588171446 39.26248010707223 30.05213778224652 5 12.793278508644129 37.436415737427595 35.596697198213064 14.173608555715218 6 28.44409843346858 38.17399361543209 18.98818405787699 14.393723893222344 7 24.7909728206442 34.25933011488593 23.821446038880886 17.128251025588987 8 23.443676218108255 35.53375833477514 22.542988141661233 18.47957730545537 9 24.803983383713444 17.35709877108021 23.315538091189943 34.52337975401641 10-11 22.78324969493403 27.24756883011881 29.981558793510892 19.987622681436267 12-13 23.970070166244504 25.660797990259077 29.80472405575035 20.56440778774607 14-15 21.437917521708425 27.201682272998372 28.122423957084735 23.237976248208465 16-17 19.992929599607383 30.149767716551807 29.0462861682173 20.811016515623507 18-19 19.833113724237524 28.895399522183844 31.451266745193557 19.820220008385075 20-21 20.992053984986427 28.339705451738272 30.76759902571474 19.90064153756056 22-23 20.548725901619704 28.38309144821573 30.75840219377211 20.309780456392456 24-25 20.529324099091415 28.179942900365212 31.015702815622763 20.275030184920602 26-27 20.25984270970029 28.334388758844565 30.993478552117075 20.41228997933807 28-29 20.249515122922894 28.460682730221965 31.0191529565645 20.270649190290644 30-31 20.51025213104225 28.512871112501394 30.74366427785829 20.233212478598062 32-33 19.973297286055942 28.61033586357492 30.921742747218467 20.49462410315067 34-35 20.51995860080046 28.310997725712788 30.794949697970992 20.37409397551576 36-37 20.711343039921665 28.25913867187337 30.49504603068378 20.534472257521188 38-39 20.509685017666072 28.218775319284 30.733622338603922 20.537917324446003 40-41 20.6101777005812 27.990179704723094 30.86203153183245 20.537611062863252 42-43 20.20637140169835 28.23794941703796 30.637708179845337 20.917971001418355 44-45 20.286409340466555 28.256481886806323 30.496112037479488 20.96099673524763 46-47 20.51036783877282 28.365046837184703 30.07095125474199 21.053634069300486 48-49 20.277955100717573 28.258991387118442 30.033873323462668 21.429180188701316 50-51 20.358056778792292 28.50970081731986 29.822609090855927 21.30963331303192 52-53 20.17493948804842 28.36972405547465 30.055703580419273 21.39963287605765 54-55 20.150242523379323 28.207148215786493 29.96696736335399 21.675641897480197 56-57 20.27090121385193 28.436859737584808 29.546473915923233 21.74576513264003 58-59 20.18971015715096 28.024377229102697 29.719977520039482 22.065935093706866 60-61 20.232377781692108 27.832824238214776 29.843464729684264 22.091333250408848 62-63 20.372668939744504 27.715540067590933 29.92744749841714 21.984343494247423 64-65 20.222352027850828 27.620855029118523 29.921005196960543 22.235787746070105 66-67 20.049271622353405 27.732502557177142 29.68272618482541 22.53549963564404 68-69 20.1969405843529 27.680916805056064 29.739248459886852 22.38289415070418 70-71 20.27390917721942 27.425768557024995 29.505368867255928 22.794953398499658 72-73 19.97717018988352 27.360591502664455 29.594603741515073 23.067634565936952 74-75 19.940456295622877 27.34949463889409 29.625541458327763 23.08450760715527 76-77 19.94462957873201 27.16884887500637 29.744141537088908 23.14238000917271 78-79 19.890608335581355 27.077652380383665 29.874414226762624 23.157325057272356 80-81 19.973824372732913 27.057446899140313 29.714598800464714 23.25412992766206 82-83 19.783534451217985 26.934231695153958 29.826286271804015 23.455947581824045 84-85 19.84869342082842 26.467207719525682 29.843748433809008 23.84035042583689 86-87 19.83549160791665 26.44732214388887 29.841414645190017 23.87577160300446 88-89 20.035258927527032 26.038634426749997 29.926724930234112 23.999381715488862 90-91 20.062315603716876 25.865069756210463 30.06815612490193 24.004458515170732 92-93 20.183578229644084 25.82837900506783 29.948486127184538 24.039556638103555 94-95 20.059128185159125 25.54227297567856 29.7966445005607 24.601954338601615 96-97 20.198667472233435 25.142624593351737 29.818839398900117 24.839868535514714 98-99 20.326323459125593 24.90800375672216 29.864186472457945 24.901486311694303 100-101 20.719198109897306 24.525970532309863 30.104463596475107 24.650367761317725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 733.0 1 460.5 2 232.0 3 291.0 4 257.0 5 255.0 6 417.0 7 691.0 8 975.5 9 1066.5 10 939.5 11 855.0 12 888.0 13 939.0 14 962.0 15 992.0 16 1027.5 17 1083.5 18 1153.5 19 1251.0 20 1428.5 21 1671.5 22 2007.0 23 2426.5 24 2919.0 25 3622.5 26 4641.0 27 5850.5 28 7048.5 29 8304.0 30 9711.0 31 11291.0 32 12861.0 33 14439.5 34 16246.0 35 18170.5 36 20272.0 37 21922.5 38 22486.0 39 22348.5 40 22096.0 41 21985.5 42 21973.5 43 21758.0 44 21296.0 45 20966.0 46 20730.0 47 20599.0 48 21219.5 49 20567.0 50 18515.0 51 17049.0 52 15865.0 53 14915.0 54 13771.0 55 12459.0 56 11300.0 57 9975.0 58 8757.5 59 7791.0 60 6553.0 61 5366.0 62 4399.5 63 3379.0 64 2587.0 65 2073.0 66 1658.0 67 1352.0 68 1100.0 69 866.0 70 698.5 71 577.5 72 465.5 73 391.5 74 322.0 75 265.0 76 212.0 77 170.0 78 135.5 79 106.0 80 82.0 81 63.0 82 50.0 83 36.0 84 26.5 85 16.5 86 12.0 87 10.5 88 6.5 89 3.5 90 2.0 91 1.5 92 2.5 93 2.0 94 3.0 95 3.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16429481274049734 2 0.09461917859245242 3 0.08896530851838672 4 0.10675837022206405 5 0.1292075602220308 6 0.03675015548142704 7 0.03990967111105199 8 0.04356805762956508 9 0.04340176733326904 10-11 0.04298604159252891 12-13 0.04747587959252226 14-15 0.04431636396289731 16-17 0.04331862218512101 18-19 0.04822418592585448 20-21 0.05021966948140709 22-23 0.06385547377768318 24-25 0.0795699067776599 26-27 0.0822305515183967 28-29 0.0719205531480416 30-31 0.06003079696287403 32-33 0.049138782555482756 34-35 0.30422809707362336 36-37 0.48199242381410073 38-39 0.4577971857030255 40-41 0.4320221897771377 42-43 0.46178815281413066 44-45 0.47292960266596606 46-47 0.4736779089992983 48-49 0.49088895466593946 50-51 0.47850032759188366 52-53 0.4859833909252059 54-55 0.4754239571104068 56-57 0.45339049285118016 58-59 0.43277049611047 60-61 0.40233937188829283 62-63 0.4584623468882097 64-65 0.5034438720362913 66-67 0.505855081332584 68-69 0.5165808054436791 70-71 0.5145021767399784 72-73 0.5017809690733307 74-75 0.49454734118445254 76-77 0.472846457517818 78-79 0.44624001011045 80-81 0.4505635578141473 82-83 0.46345105577709117 84-85 0.46095670133265043 86-87 0.4735116187030022 88-89 0.4868979875548342 90-91 0.4909720998140875 92-93 0.49479677662889654 94-95 0.4983720179992617 96-97 0.5024461302585149 98-99 0.492884438221492 100-101 0.4775857309622555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.05718914830925 #Duplication Level Percentage of deduplicated Percentage of total 1 91.65324550431883 76.12460947908635 2 6.046648817327226 10.044353090682955 3 0.9828368404713926 2.4489499608287733 4 0.433226392206608 1.439302656061754 5 0.22591586063575156 0.9381968184213345 6 0.13454407378968644 0.6704911551320439 7 0.09002511117718869 0.5234062880998931 8 0.07270962703707598 0.4831245796577152 9 0.053946150199031234 0.40325540408136346 >10 0.28727030029034434 4.116160984389356 >50 0.013421444899193252 0.7730013307589033 >100 0.005208284478784881 0.8899614130742391 >500 4.006372675344165E-4 0.18983846929051384 >1k 6.009559013016247E-4 0.9553483704348208 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.42054815933271034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1857 0.3088010802217647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1356 0.2254896417774437 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.6629029629604993E-4 0.0 3 0.0 0.0 0.0 1.6629029629604993E-4 0.0 4 0.0 0.0 0.0 1.6629029629604993E-4 0.0 5 0.0 0.0 0.0 1.6629029629604993E-4 0.0 6 0.0 0.0 0.0 1.6629029629604993E-4 0.0 7 0.0 0.0 0.0 1.6629029629604993E-4 0.0 8 0.0 0.0 0.0 1.6629029629604993E-4 0.0 9 0.0 0.0 0.0 1.6629029629604993E-4 0.0 10-11 0.0 0.0 0.0 1.6629029629604993E-4 0.0 12-13 0.0 0.0 0.0 1.6629029629604993E-4 0.0 14-15 0.0 0.0 0.0 1.6629029629604993E-4 0.0 16-17 0.0 0.0 0.0 2.494354444440749E-4 0.0 18-19 0.0 0.0 0.0 4.988708888881498E-4 0.0 20-21 0.0 0.0 0.0 6.651611851841998E-4 0.0 22-23 0.0 0.0 0.0 0.0010808869259243247 0.0 24-25 0.0 1.6629029629604993E-4 0.0 0.0024943544444407492 0.0 26-27 0.0 3.3258059259209987E-4 0.0 0.006485321555545948 0.0 28-29 0.0 3.3258059259209987E-4 0.0 0.027853624629588368 0.0 30-31 0.0 3.3258059259209987E-4 0.0 0.0828957127035809 0.0 32-33 0.0 3.3258059259209987E-4 0.0 0.19588996903674682 0.0 34-35 0.0 3.3258059259209987E-4 0.0 0.36018478177724417 0.0 36-37 0.0 3.3258059259209987E-4 0.0 0.6182673216287137 0.0 38-39 0.0 3.3258059259209987E-4 0.0 1.0102966951466514 0.0 40-41 0.0 3.3258059259209987E-4 0.0 1.4589479145533941 0.0 42-43 0.0 3.3258059259209987E-4 0.0 1.874590510145371 0.0 44-45 0.0 3.3258059259209987E-4 0.0 2.3439448714409723 0.0 46-47 1.6629029629604993E-4 3.3258059259209987E-4 0.0 2.8805636575883247 0.0 48-49 1.6629029629604993E-4 3.3258059259209987E-4 0.0 3.4634111461059804 0.0 50-51 1.6629029629604993E-4 3.3258059259209987E-4 0.0 4.12059039706797 0.0 52-53 1.6629029629604993E-4 3.3258059259209987E-4 0.0 4.730293768437436 1.6629029629604993E-4 54-55 1.6629029629604993E-4 3.3258059259209987E-4 0.0 5.362862055547611 1.6629029629604993E-4 56-57 1.6629029629604993E-4 3.3258059259209987E-4 0.0 6.070593556583599 1.6629029629604993E-4 58-59 1.6629029629604993E-4 3.3258059259209987E-4 0.0 6.836609806471353 1.6629029629604993E-4 60-61 1.6629029629604993E-4 3.3258059259209987E-4 0.0 7.70397999195155 1.6629029629604993E-4 62-63 1.6629029629604993E-4 3.3258059259209987E-4 0.0 8.541417924098457 1.6629029629604993E-4 64-65 3.3258059259209987E-4 3.3258059259209987E-4 0.0 9.377026662986108 1.6629029629604993E-4 66-67 3.3258059259209987E-4 3.3258059259209987E-4 0.0 10.27491111783663 1.6629029629604993E-4 68-69 3.3258059259209987E-4 4.988708888881498E-4 0.0 11.272154024724042 1.6629029629604993E-4 70-71 3.3258059259209987E-4 4.988708888881498E-4 0.0 12.360274578537243 1.6629029629604993E-4 72-73 3.3258059259209987E-4 4.988708888881498E-4 0.0 13.468848838794862 1.6629029629604993E-4 74-75 3.3258059259209987E-4 4.988708888881498E-4 0.0 14.562789552978426 1.6629029629604993E-4 76-77 3.3258059259209987E-4 4.988708888881498E-4 0.0 15.679761473198994 1.6629029629604993E-4 78-79 3.3258059259209987E-4 4.988708888881498E-4 0.0 16.88470096016017 1.6629029629604993E-4 80-81 3.3258059259209987E-4 4.988708888881498E-4 0.0 18.189913495787867 1.6629029629604993E-4 82-83 3.3258059259209987E-4 4.988708888881498E-4 0.0 19.504271997711847 1.6629029629604993E-4 84-85 4.1572574074012487E-4 4.988708888881498E-4 0.0 20.8322663039321 1.6629029629604993E-4 86-87 4.988708888881498E-4 4.988708888881498E-4 0.0 22.14695738644867 1.6629029629604993E-4 88-89 4.988708888881498E-4 4.988708888881498E-4 0.0 23.4970683020763 1.6629029629604993E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCCGA 90 2.4420091E-5 31.607616 1 CGTGCAC 105 7.0164606E-5 27.07645 3 GTATCAA 3475 0.0 26.332102 1 GGTATCA 2485 0.0 24.99355 1 GTACATG 1840 0.0 24.478725 1 GCGCCGT 70 1.7605396E-5 23.782999 94-95 AGCACCG 120 1.7381177E-4 23.691893 5 TACATGG 1900 0.0 23.193117 2 GAGTACT 2080 0.0 22.757923 12-13 ATCAACG 3950 0.0 22.672243 3 CAACGCA 3960 0.0 22.614988 5 TATCAAC 4030 0.0 22.457327 2 TCAACGC 3990 0.0 22.444952 4 AACGCAG 3980 0.0 22.358086 6 GTACTAA 220 5.740185E-8 21.550646 1 AGTACTT 2165 0.0 21.427137 12-13 ACATGGG 1940 0.0 21.249428 3 CGCAGAG 4230 0.0 20.814623 8 GACTGTA 205 7.0477836E-7 20.78187 7 CATGGGG 845 0.0 20.74793 4 >>END_MODULE