FastQCFastQC Report
Thu 26 May 2016
SRR938843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601358
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26780.4453254134808218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.39760009844385547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.31977623977730407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.22898173799966076No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT13010.21634367548116099TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG7540.12538288340722165TruSeq Adapter, Index 3 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.11390885296279421No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT6710.1115807888146495RNA PCR Primer, Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.026.5878581
CCTGCGT909.005883E-426.3570168
GTACATG18250.022.1906641
GTATCAA30650.022.0735471
GTATTAG1805.027752E-621.1754071
GTCTAAC1353.7159206E-421.1754061
TACATGG18550.020.7161882
CGCAGAA1456.1915495E-419.6314332
GAGTACT20900.019.06943712-13
AGACCGT1250.00607009518.977056
AGTACTT21950.018.91378212-13
ACATGGG18650.018.5700383
CTATACG650.00760891918.25019840-41
ATCAACG37200.018.109823
GTCATAT1851.4848229E-418.027711
TCAACGC37600.017.9171624
TATCAAC38400.017.7909852
CAACGCA37700.017.7437935
GGGTAAG2152.3296256E-517.7282491
AACGCAG38500.017.3750936
Produced by FastQC (version 0.11.5)