##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938843_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601358 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933477229869727 31.0 31.0 34.0 30.0 34.0 2 32.16032546336791 33.0 31.0 34.0 30.0 34.0 3 32.25659424169962 34.0 31.0 34.0 30.0 34.0 4 35.90697720825199 37.0 35.0 37.0 35.0 37.0 5 35.807676957818806 37.0 35.0 37.0 35.0 37.0 6 35.83047203163507 37.0 35.0 37.0 35.0 37.0 7 35.7572460996611 37.0 35.0 37.0 33.0 37.0 8 35.7322975665078 37.0 35.0 37.0 33.0 37.0 9 37.37167211544538 39.0 37.0 39.0 34.0 39.0 10-11 37.3059483701888 39.0 37.0 39.0 34.0 39.0 12-13 37.24325609703371 39.0 37.0 39.0 33.0 39.0 14-15 38.460312326434504 40.0 38.0 41.0 33.0 41.0 16-17 38.40422094659088 40.0 38.0 41.0 33.0 41.0 18-19 38.43208787444418 40.0 38.0 41.0 33.5 41.0 20-21 38.489529531493716 40.0 38.0 41.0 34.0 41.0 22-23 38.428628204829735 40.0 38.0 41.0 34.0 41.0 24-25 38.35922029805873 40.0 38.0 41.0 34.0 41.0 26-27 38.190335873140455 40.0 38.0 41.0 33.0 41.0 28-29 38.02225795615922 40.0 37.0 41.0 33.0 41.0 30-31 37.78894103013512 40.0 37.0 41.0 33.0 41.0 32-33 37.56048643237473 40.0 36.5 41.0 32.0 41.0 34-35 37.503717419573704 40.0 36.0 41.0 32.0 41.0 36-37 37.39318259672275 40.0 36.0 41.0 32.0 41.0 38-39 37.28157353855774 40.0 36.0 41.0 31.0 41.0 40-41 37.12799364105907 40.0 36.0 41.0 31.0 41.0 42-43 36.96301620665228 40.0 36.0 41.0 31.0 41.0 44-45 36.72525683536263 40.0 36.0 41.0 30.5 41.0 46-47 36.422861257354185 39.0 35.0 41.0 30.0 41.0 48-49 36.152950488727186 39.0 35.0 41.0 29.5 41.0 50-51 35.91603919794864 39.0 35.0 41.0 28.5 41.0 52-53 35.726175589249664 39.0 35.0 41.0 28.0 41.0 54-55 35.50788631730184 39.0 35.0 40.0 28.0 41.0 56-57 35.23150103598854 38.5 35.0 40.0 27.0 41.0 58-59 34.923360128243075 38.0 34.0 40.0 26.0 41.0 60-61 34.56754379254953 38.0 34.0 40.0 26.0 41.0 62-63 34.1886147685738 37.5 34.0 40.0 24.5 41.0 64-65 33.806533712031765 37.0 33.0 40.0 23.0 41.0 66-67 33.4002740464083 36.5 33.0 39.5 22.5 41.0 68-69 32.950942699689705 36.0 32.5 39.0 21.5 40.5 70-71 32.49184512386964 35.5 32.0 39.0 19.5 40.0 72-73 32.166124837451235 35.0 32.0 38.0 18.5 40.0 74-75 31.75011806611037 35.0 31.0 37.5 17.5 40.0 76-77 29.630364109232772 33.0 28.5 35.5 11.0 38.5 78-79 30.687437433276017 34.5 30.5 36.0 11.0 39.0 80-81 30.728788841255955 35.0 31.0 36.0 9.0 38.5 82-83 30.405317631094952 34.0 31.0 36.0 7.0 37.0 84-85 29.98909301946594 34.0 30.0 35.0 2.0 37.0 86-87 29.446343941545635 34.0 29.5 35.0 2.0 36.5 88-89 28.92014324246123 34.0 29.0 35.0 2.0 36.0 90-91 28.499682385534072 34.0 29.0 35.0 2.0 36.0 92-93 28.216407697245238 34.0 29.0 35.0 2.0 35.0 94-95 27.838192058640608 33.5 28.5 35.0 2.0 35.0 96-97 27.351687347636517 33.0 27.0 35.0 2.0 35.0 98-99 26.662715886377168 33.0 25.5 35.0 2.0 35.0 100-101 23.82258155707582 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09025416949435794 1101 2 -0.035949310690696734 1101 3 0.20475886387197306 1101 4 0.10562138896301576 1101 5 0.11069886286428243 1101 6 0.35235944913667083 1101 7 0.04371283556023542 1101 8 0.14097467169688827 1101 9 0.21414023874390153 1101 10-11 0.12393969145932004 1101 12-13 0.21047138463768533 1101 14-15 0.33728918845275757 1101 16-17 0.2820915035554279 1101 18-19 0.436370650273723 1101 20-21 0.43199106438370904 1101 22-23 0.31748857091665883 1101 24-25 0.17594210719561687 1101 26-27 0.43745637166141904 1101 28-29 0.3030288431805275 1101 30-31 0.43598526057861164 1101 32-33 0.40379785853610883 1101 34-35 0.1662154412183412 1101 36-37 0.08554442374089177 1101 38-39 0.30804356463242044 1101 40-41 0.42248966954070966 1101 42-43 0.3541877358701768 1101 44-45 0.29112338880969446 1101 46-47 0.049752007026597767 1101 48-49 -0.07741649690407115 1101 50-51 -0.2542478386392446 1101 52-53 -0.09550306479028592 1101 54-55 -0.1722031180582917 1101 56-57 -0.3135237265446946 1101 58-59 -0.25723264584188144 1101 60-61 -0.22153611388966254 1101 62-63 -0.2747706925356894 1101 64-65 -0.502312105411896 1101 66-67 -0.4929209334796525 1101 68-69 -0.2819544809137611 1101 70-71 -0.4557512552214149 1101 72-73 -0.4931677350364083 1101 74-75 -0.564633513392927 1101 76-77 -0.593823319872218 1101 78-79 -0.5765643769230842 1101 80-81 -0.7890148694647507 1101 82-83 -0.596858749005122 1101 84-85 -0.7062971481716573 1101 86-87 -0.3285582733538739 1101 88-89 -0.19314991834143314 1101 90-91 -0.11882896665520448 1101 92-93 -0.3144218974500248 1101 94-95 -0.1972701176438214 1101 96-97 -0.33185472089138557 1101 98-99 -0.22957229744418228 1101 100-101 0.7567578663870158 1102 1 0.13032377657216188 1102 2 0.07700931538268208 1102 3 0.03452727396825139 1102 4 0.15483424350374264 1102 5 0.12259386676748107 1102 6 -0.06264835726457818 1102 7 0.07757635364506399 1102 8 0.13772850984416607 1102 9 0.11097539357086106 1102 10-11 0.3391377788910148 1102 12-13 0.25359687253776286 1102 14-15 0.13865205600414754 1102 16-17 0.09292288528845205 1102 18-19 0.21765968227996524 1102 20-21 0.22151097070690184 1102 22-23 0.15751426698743387 1102 24-25 0.16904645275897678 1102 26-27 0.0849323019242334 1102 28-29 -0.0029837121148190704 1102 30-31 0.0816722238885248 1102 32-33 0.2610838330351939 1102 34-35 0.03515409591519614 1102 36-37 0.15236559209895262 1102 38-39 0.20516332783825675 1102 40-41 0.12473563623340311 1102 42-43 0.40597832915667453 1102 44-45 0.23494169482062688 1102 46-47 0.1573225899045596 1102 48-49 0.2304728473582216 1102 50-51 0.15894172680442153 1102 52-53 -0.04092005672367094 1102 54-55 -0.02769849927089041 1102 56-57 -0.20261102813199727 1102 58-59 -0.22574032213643846 1102 60-61 -0.1587108471709584 1102 62-63 -0.23517922753438825 1102 64-65 -0.2199675829034362 1102 66-67 -0.15591859155927779 1102 68-69 -0.09481877963870744 1102 70-71 -0.35255713604368566 1102 72-73 -0.18315765176812704 1102 74-75 -0.17312460108483307 1102 76-77 -0.2878091382432828 1102 78-79 -0.30224906336597 1102 80-81 -0.3204258764905106 1102 82-83 -0.3362469223072324 1102 84-85 -0.0809702551193574 1102 86-87 -0.21484990749387123 1102 88-89 -0.06440935107627155 1102 90-91 -0.3498268849482038 1102 92-93 -0.1719209216498676 1102 94-95 0.09377691592370496 1102 96-97 0.14455368400062696 1102 98-99 0.4332318269378135 1102 100-101 0.7368238955309145 1103 1 -0.14500525486152327 1103 2 0.007727434472890593 1103 3 0.04761186379702309 1103 4 0.07449307304629116 1103 5 0.016961998635615316 1103 6 0.01571223563089319 1103 7 -0.017494563809577812 1103 8 0.057029557633590855 1103 9 -0.102776842565099 1103 10-11 -0.13551562003564754 1103 12-13 -0.0012699176487913633 1103 14-15 0.13353769362234402 1103 16-17 -0.07696594680968616 1103 18-19 0.11334553712279671 1103 20-21 -0.08218823706207701 1103 22-23 -0.06677815570615309 1103 24-25 -0.0034554639289865463 1103 26-27 0.02647331904665151 1103 28-29 -0.018107977384431706 1103 30-31 -0.1537290027917706 1103 32-33 -0.1953562385211498 1103 34-35 -0.06755322583283174 1103 36-37 -0.13909396578502964 1103 38-39 -0.3278532195678281 1103 40-41 -0.42478998349570674 1103 42-43 -0.29005891847941 1103 44-45 -0.10757843040298809 1103 46-47 0.1334071158437382 1103 48-49 0.10597597794856739 1103 50-51 -0.011710585999061607 1103 52-53 0.08113782725281737 1103 54-55 0.10918017438103789 1103 56-57 0.021101596452716365 1103 58-59 -0.07771451073853086 1103 60-61 0.03441143752110065 1103 62-63 0.07068583763300751 1103 64-65 0.11211777360052366 1103 66-67 -0.16527202881969316 1103 68-69 -0.33205044332385825 1103 70-71 -0.23150551324788182 1103 72-73 -0.020317439654661484 1103 74-75 -0.13888137338230422 1103 76-77 -0.36400067926040336 1103 78-79 -0.18606742523665787 1103 80-81 6.417187049940765E-4 1103 82-83 -0.06857346204656167 1103 84-85 -0.21787129013085504 1103 86-87 -0.1884107942815021 1103 88-89 -0.17909118087438358 1103 90-91 0.001176181752533978 1103 92-93 -0.067958169285955 1103 94-95 -0.18623362586009407 1103 96-97 -0.24535495387312523 1103 98-99 -0.12643307211662247 1103 100-101 0.4937670976776012 1104 1 -0.11991720217228163 1104 2 -0.16154728372755045 1104 3 -0.15346086246279356 1104 4 -0.09881133237367123 1104 5 -0.07659669180888784 1104 6 0.012672947728006534 1104 7 -0.24490881689374078 1104 8 -0.054325370531515205 1104 9 -0.2371647448374432 1104 10-11 -0.05885097543424678 1104 12-13 0.03641632384769622 1104 14-15 -0.1287169504219534 1104 16-17 -0.16240336285786583 1104 18-19 0.012830838977940573 1104 20-21 -0.09021666152246155 1104 22-23 -0.1689038002394554 1104 24-25 -0.08750322593603244 1104 26-27 0.09827881700085328 1104 28-29 -0.17741215996454685 1104 30-31 -0.10672372272541253 1104 32-33 -0.40639021540770415 1104 34-35 -0.07134924071358029 1104 36-37 -0.18990764072358957 1104 38-39 0.08009844894552032 1104 40-41 -0.05549483830935742 1104 42-43 -0.10522100351757047 1104 44-45 -0.2521906581205471 1104 46-47 -0.4497059568082449 1104 48-49 -0.5659155609462729 1104 50-51 -0.44641541605272295 1104 52-53 -0.2781976148057268 1104 54-55 -0.3447801848863108 1104 56-57 -0.216445679639655 1104 58-59 -0.21005816692141366 1104 60-61 -0.06649013535682258 1104 62-63 -0.28554996406428756 1104 64-65 -0.18959190321979946 1104 66-67 -0.28634370278023624 1104 68-69 -0.06276860662833172 1104 70-71 -0.28944542076934 1104 72-73 -0.3535697872105388 1104 74-75 -0.3894833504669428 1104 76-77 -0.17943667160956878 1104 78-79 -0.16473979495272317 1104 80-81 0.07389567565975952 1104 82-83 0.020447961528656577 1104 84-85 -0.003983725213277722 1104 86-87 -0.021995854107807133 1104 88-89 -0.0913495389161163 1104 90-91 -0.19116400803273237 1104 92-93 0.11860984948211595 1104 94-95 -0.09659320271198624 1104 96-97 0.0636898678859481 1104 98-99 0.049751228816212745 1104 100-101 0.5822174941468283 1105 1 -0.13398950448640434 1105 2 -0.24244670029974813 1105 3 -0.25917524992295427 1105 4 -0.20717874350924603 1105 5 -0.028162687998502633 1105 6 -0.14580812202812155 1105 7 -0.10368130379183782 1105 8 -0.24383850804360208 1105 9 -0.1905469048854016 1105 10-11 -0.11863451956524784 1105 12-13 -0.31789142689864747 1105 14-15 -0.14907460412817386 1105 16-17 -0.31229029775492023 1105 18-19 -0.290630258910916 1105 20-21 -0.20702982811691584 1105 22-23 -0.16713808537718222 1105 24-25 -0.3209069900246533 1105 26-27 -0.22877757310639169 1105 28-29 -0.36241307991682703 1105 30-31 -0.19610895719453936 1105 32-33 -0.17725235907457915 1105 34-35 -0.10205338999512747 1105 36-37 -0.11556659363983357 1105 38-39 -0.1601990220759788 1105 40-41 -0.3113691787629236 1105 42-43 -0.3788400364399891 1105 44-45 -0.3383323189015002 1105 46-47 -0.26037680975238686 1105 48-49 -0.2340961920928919 1105 50-51 -0.3237739066187615 1105 52-53 -0.3982068736803015 1105 54-55 -0.6011961101505747 1105 56-57 -0.29741928591194977 1105 58-59 -0.5287726671499158 1105 60-61 -0.6106374582956775 1105 62-63 -0.6931674657113049 1105 64-65 -0.6284091413449957 1105 66-67 -0.5371241784252376 1105 68-69 -0.44115239884968815 1105 70-71 -0.7150969547155839 1105 72-73 -1.0221569779136814 1105 74-75 -0.9677475488917437 1105 76-77 -0.9951939703922648 1105 78-79 -1.2495000435178945 1105 80-81 -0.9207107331432915 1105 82-83 -0.9639959421553073 1105 84-85 -0.912639882661626 1105 86-87 -0.7293056168788254 1105 88-89 -0.37321846648916335 1105 90-91 -0.16860292023367762 1105 92-93 -0.456053180900053 1105 94-95 -0.6307590433999906 1105 96-97 -0.4659860121522783 1105 98-99 -0.1549129292493987 1105 100-101 0.22478487323245488 1106 1 -0.06782882643795674 1106 2 -0.14433290026005352 1106 3 0.012124115813797687 1106 4 -0.07952919098922706 1106 5 0.0032779279567236586 1106 6 -0.24791235923842692 1106 7 -0.20610548696682685 1106 8 -0.16779209217902746 1106 9 0.002620471291066906 1106 10-11 -0.22684883596218697 1106 12-13 -0.05825083286563881 1106 14-15 -0.0680497698038991 1106 16-17 -0.2535550323034599 1106 18-19 -0.28161400387843827 1106 20-21 -0.19456971027015868 1106 22-23 -0.163366870441628 1106 24-25 -0.17353928619981218 1106 26-27 -0.17518366155960763 1106 28-29 -0.2998147631880954 1106 30-31 -0.5881094193387071 1106 32-33 -0.47838076109260896 1106 34-35 -0.40292109530118836 1106 36-37 -0.2093063175975729 1106 38-39 -0.32217907285259173 1106 40-41 -0.2189403445215916 1106 42-43 -0.1456423838953853 1106 44-45 -0.20563473004984445 1106 46-47 -0.08100858453413196 1106 48-49 -0.13950772258996125 1106 50-51 -0.05436014834978664 1106 52-53 -0.08388514431311478 1106 54-55 -0.02301523995679844 1106 56-57 -0.05157784283715472 1106 58-59 0.03042178001011564 1106 60-61 -7.835272239731239E-5 1106 62-63 0.00904727135532113 1106 64-65 -0.01716718813412399 1106 66-67 -0.2554991491709231 1106 68-69 -0.36746558447463684 1106 70-71 -0.1974436398680126 1106 72-73 -0.06489494513106564 1106 74-75 0.19700092321815177 1106 76-77 0.11759735077719569 1106 78-79 -0.1830179382734478 1106 80-81 -0.29548447505947095 1106 82-83 -0.20664385050856282 1106 84-85 -0.008386675055206183 1106 86-87 0.16026266344124096 1106 88-89 0.44083367877815505 1106 90-91 0.42132411431159866 1106 92-93 0.4762856537263964 1106 94-95 0.28547288187485265 1106 96-97 0.34096066648274004 1106 98-99 0.43876013929902413 1106 100-101 0.9846757953582035 1107 1 -0.15669359794521753 1107 2 -0.13698682361345504 1107 3 -0.06860979166881975 1107 4 0.040210691380188734 1107 5 -0.14000265832904546 1107 6 -0.07567924529546843 1107 7 -0.1899429513742419 1107 8 -0.25493558281992534 1107 9 -0.04679487669941551 1107 10-11 -0.202923031919795 1107 12-13 -0.07569849425760111 1107 14-15 -0.22032477797268513 1107 16-17 -0.3944564583408905 1107 18-19 -0.2131318235115387 1107 20-21 -0.251341345895419 1107 22-23 -0.18768360946044282 1107 24-25 -0.25373347002094704 1107 26-27 -0.2626787019367711 1107 28-29 -0.01051266964377362 1107 30-31 -0.2031974672311634 1107 32-33 -0.16492968047831624 1107 34-35 -0.25415574964465293 1107 36-37 -0.19454560481224092 1107 38-39 0.2180011656760925 1107 40-41 0.10094219993995779 1107 42-43 -0.13757862065027382 1107 44-45 -0.16539614301721173 1107 46-47 -0.23406254523057868 1107 48-49 -0.20364877426339945 1107 50-51 -0.15840865929596504 1107 52-53 -0.011142064445685662 1107 54-55 -0.13155950313189635 1107 56-57 -0.2841506034215726 1107 58-59 -0.289736461132577 1107 60-61 -0.5853517738118867 1107 62-63 -0.4384128182679774 1107 64-65 -0.19943186861772233 1107 66-67 -0.1697458116364956 1107 68-69 -0.13772418504411377 1107 70-71 -0.43426563025218456 1107 72-73 -0.4599916286021042 1107 74-75 -0.2838388867991206 1107 76-77 -0.2795079799421245 1107 78-79 -0.38123072359762844 1107 80-81 -0.17643456374920063 1107 82-83 -0.11858282964313815 1107 84-85 0.04045831630921626 1107 86-87 0.04200337435941037 1107 88-89 0.05709161417469133 1107 90-91 -0.1627371166084366 1107 92-93 -0.05483597956492403 1107 94-95 0.05970355685034434 1107 96-97 0.04351603921298164 1107 98-99 0.09165364160962497 1107 100-101 0.5016887603957763 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14 2796.0 15 3464.0 16 4376.0 17 5715.0 18 5441.0 19 3949.0 20 3831.0 21 4155.0 22 4536.0 23 4800.0 24 5673.0 25 6442.0 26 6893.0 27 8400.0 28 10050.0 29 12248.0 30 14834.0 31 18176.0 32 22496.0 33 27648.0 34 36459.0 35 49662.0 36 72517.0 37 116993.0 38 124535.0 39 18155.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.539605390360336 22.456717997866164 14.976766551849074 23.02691005992443 2 14.984252308940766 23.006096202262214 39.95340545897785 22.056246029819178 3 17.400616604418666 27.025665244330334 32.52239098839627 23.05132716285474 4 12.522657052870336 19.33108065411951 38.48423069120225 29.662031601807907 5 12.440343356203792 38.13219413394351 35.486182939280766 13.941279570571938 6 28.181549093884172 38.88881498209054 18.554172389824362 14.375463534200925 7 24.428210816186034 34.71176902943006 23.401035655965334 17.458984498418577 8 23.428806135446774 36.17395960476122 22.266270674041085 18.130963585750916 9 24.128223121667958 17.516520940937014 22.96535507966968 35.38990085772535 10-11 22.5372407118555 27.722089005218187 29.84935762058541 19.8913126623409 12-13 23.789406642964757 25.769258910665528 29.645901443067192 20.795433003302524 14-15 21.218016993304282 27.480795437632878 27.691819051823842 23.609368517238998 16-17 20.056306924288307 30.647280189308333 28.5449166620936 20.751496224309758 18-19 19.981042890460333 28.88074065759552 31.069538502471495 20.06867794947265 20-21 20.871843394450558 28.547387745735485 30.36801705473279 20.212751805081165 22-23 20.493200389784455 28.874647048846114 30.328190528769884 20.30396203259955 24-25 20.767579378672938 28.68773675580935 30.665011523917535 19.879672341600177 26-27 20.497059151219656 28.67830589816033 30.755690879128373 20.068944071491643 28-29 20.300303646081037 28.729309329883364 30.788814649509945 20.181572374525658 30-31 20.445142494154897 28.92785994366085 30.644890398065712 19.982107164118545 32-33 20.246259299784818 29.03046105647551 30.584443875362098 20.13883576837757 34-35 20.862221217024572 28.391336378860473 30.69468950756587 20.051752896549086 36-37 20.53945048730998 28.812244286997306 30.28756278481855 20.360742440874162 38-39 20.46619845032921 28.208981039067737 30.68482875954824 20.63999175105482 40-41 20.529698347509456 27.98207896332058 30.972327664269933 20.51589502490003 42-43 20.288710266168547 28.21439826251664 30.875751148081044 20.62114032323377 44-45 20.16421521755398 28.400585354497004 30.446831685638028 20.988367742310988 46-47 20.74329610866245 28.304718478552786 29.726938705259933 21.22504670752484 48-49 20.312193402266203 28.203249977550808 29.937075751881576 21.54748086830141 50-51 20.282246631972708 28.483246543006736 29.85614950274672 21.378357322273835 52-53 20.086121744451724 28.516873476365163 30.189005550770094 21.207999228413023 54-55 19.965644424785236 28.439049617698608 29.80645472414103 21.788851233375127 56-57 20.207097935007102 28.68499296592047 29.211634334290054 21.896274764782376 58-59 19.79288440272385 28.528157244888625 29.63282919407006 22.046129158317466 60-61 20.14498971485966 28.718157222130763 29.246133302735664 21.890719760273917 62-63 19.90985403037791 28.83174415240173 29.285051500104764 21.973350317115596 64-65 19.72934591374855 28.719165621809307 29.149441763475338 22.402046700966814 66-67 19.637449675813293 28.68906844754561 28.991637163653174 22.68184471298792 68-69 19.896816356395142 28.55243574926624 29.026365458007337 22.524382436331287 70-71 20.277175892282546 28.266104155476583 28.530259365994237 22.926460586246638 72-73 20.0703241663036 28.277748030291438 28.571167258105824 23.08076054529914 74-75 20.022349415822188 28.358481969143174 28.440463085217125 23.178705529817513 76-77 19.97073689589709 28.204522131678978 28.711632749708077 23.11310822271586 78-79 20.1346457439535 27.95412995127534 28.67626631710453 23.234957987666625 80-81 20.157643331961435 28.126613536872824 28.309034143583478 23.406708987582263 82-83 19.935712171451947 27.974517674995592 28.444370907180083 23.645399246372378 84-85 19.996823855340747 27.5827377369221 28.297370285254374 24.12306812248278 86-87 19.978265847819298 27.75301342770896 28.19910481940252 24.06961590506922 88-89 20.25271580232846 27.308421552246926 28.187037721353636 24.25182492407098 90-91 20.118944589797078 27.176579271658234 28.25972042268435 24.44475571586033 92-93 20.018358448711083 27.32058108481138 28.133408053106468 24.527652413371072 94-95 19.988343050229645 26.872511881441667 28.162009984069392 24.977135084259295 96-97 20.013386379981956 26.69701467097359 28.10599352298757 25.183605426056882 98-99 20.14577031613501 26.634844194047798 28.05529826708594 25.164087222731258 100-101 20.357211749524403 26.46345235402892 28.20561002540941 24.973725871037264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 561.0 1 372.0 2 223.5 3 267.0 4 222.5 5 211.0 6 353.5 7 623.0 8 870.0 9 919.0 10 773.0 11 654.0 12 651.0 13 679.0 14 742.0 15 774.5 16 798.0 17 878.0 18 983.5 19 1095.5 20 1243.0 21 1476.0 22 1828.0 23 2260.0 24 2732.5 25 3428.5 26 4506.5 27 5691.0 28 6868.5 29 8227.5 30 9637.0 31 11125.0 32 12753.0 33 14358.0 34 16108.5 35 18003.0 36 20120.0 37 21666.0 38 22296.0 39 22429.5 40 22362.0 41 22405.0 42 22282.0 43 22361.0 44 22595.5 45 22294.0 46 21897.0 47 21695.5 48 21908.5 49 21043.5 50 19132.5 51 17673.5 52 16062.5 53 14764.5 54 13651.0 55 12420.0 56 11292.5 57 10014.0 58 8753.0 59 7611.0 60 6372.0 61 5154.5 62 4131.5 63 3204.0 64 2400.5 65 1854.0 66 1418.5 67 1142.0 68 893.5 69 680.5 70 552.5 71 430.5 72 386.0 73 345.0 74 267.5 75 203.0 76 154.5 77 124.0 78 82.5 79 56.0 80 43.5 81 33.0 82 27.5 83 17.0 84 11.5 85 9.5 86 8.5 87 5.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4052494520734737 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.820160370361748E-4 16-17 0.0018291932592565494 18-19 8.314514814802497E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.6629029629604993E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006485321555545948 36-37 0.016296449037012894 38-39 0.011474030444427446 40-41 0.008896530851838672 42-43 0.004739273444437423 44-45 0.002161773851848649 46-47 0.0015797578148124744 48-49 0.0 50-51 7.483063333322247E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.988708888881498E-4 60-61 8.314514814802496E-4 62-63 0.0 64-65 0.0 66-67 0.0011640320740723496 68-69 4.988708888881498E-4 70-71 0.0011640320740723496 72-73 0.0 74-75 0.0 76-77 0.10002361322207405 78-79 0.037415316666611236 80-81 8.314514814802497E-5 82-83 0.0 84-85 0.0 86-87 0.15307021774051396 88-89 0.3241829326291494 90-91 0.27720592392551524 92-93 0.0 94-95 0.0 96-97 8.314514814802497E-5 98-99 1.6629029629604993E-4 100-101 9.977417777762997E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.29784844194083 #Duplication Level Percentage of deduplicated Percentage of total 1 91.18621519628117 74.13243103021466 2 6.339472760166265 10.307709913156184 3 1.1156320317328727 2.720954514983808 4 0.4207217799743569 1.3681510201831537 5 0.22027718164105253 0.8954030464136078 6 0.14999479828417667 0.7316552626791888 7 0.10271686321133301 0.5845461984450564 8 0.07953280231477454 0.5172676566999505 9 0.055933072188156484 0.40925145830803933 >10 0.30518060341654335 4.3143645518923925 >50 0.014920813960462664 0.814889359200801 >100 0.007358162735828827 1.0676258285505396 >500 0.0010219670465113047 0.5289109746335394 >1k 0.0010219670465113047 1.6068391846391226 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2678 0.4453254134808218 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2391 0.39760009844385547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1923 0.31977623977730407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1377 0.22898173799966076 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1301 0.21634367548116099 TruSeq Adapter, Index 3 (95% over 21bp) TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 754 0.12538288340722165 TruSeq Adapter, Index 3 (96% over 28bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 685 0.11390885296279421 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 671 0.1115807888146495 RNA PCR Primer, Index 3 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.3258059259209987E-4 0.0 0.0 0.0 0.0 7 3.3258059259209987E-4 0.0 0.0 0.0 0.0 8 3.3258059259209987E-4 0.0 0.0 0.0 0.0 9 3.3258059259209987E-4 0.0 0.0 1.6629029629604993E-4 0.0 10-11 3.3258059259209987E-4 0.0 0.0 1.6629029629604993E-4 0.0 12-13 3.3258059259209987E-4 0.0 0.0 1.6629029629604993E-4 0.0 14-15 3.3258059259209987E-4 0.0 0.0 1.6629029629604993E-4 0.0 16-17 3.3258059259209987E-4 0.0 0.0 3.3258059259209987E-4 0.0 18-19 3.3258059259209987E-4 0.0 0.0 6.651611851841997E-4 0.0 20-21 3.3258059259209987E-4 1.6629029629604993E-4 0.0 8.314514814802497E-4 0.0 22-23 3.3258059259209987E-4 1.6629029629604993E-4 0.0 0.0012471772222203744 0.0 24-25 3.3258059259209987E-4 2.494354444440749E-4 0.0 0.002660644740736799 0.0 26-27 3.3258059259209987E-4 3.3258059259209987E-4 0.0 0.006568466703693973 0.0 28-29 3.3258059259209987E-4 3.3258059259209987E-4 0.0 0.02810306007403244 0.0 30-31 3.3258059259209987E-4 3.3258059259209987E-4 0.0 0.08480805111098547 0.0 32-33 3.3258059259209987E-4 4.1572574074012487E-4 0.0 0.1990494846663718 1.6629029629604993E-4 34-35 3.3258059259209987E-4 4.988708888881498E-4 0.0 0.3654229261105697 1.6629029629604993E-4 36-37 3.3258059259209987E-4 4.988708888881498E-4 0.0 0.6268312718879603 1.6629029629604993E-4 38-39 3.3258059259209987E-4 4.988708888881498E-4 0.0 1.0242650800355195 1.6629029629604993E-4 40-41 3.3258059259209987E-4 4.988708888881498E-4 0.0 1.478570169516328 1.6629029629604993E-4 42-43 4.1572574074012487E-4 4.988708888881498E-4 0.0 1.8987857482564463 1.6629029629604993E-4 44-45 4.988708888881498E-4 4.988708888881498E-4 0.0 2.3729625281446327 2.494354444440749E-4 46-47 4.988708888881498E-4 4.988708888881498E-4 0.0 2.913073410514203 3.3258059259209987E-4 48-49 4.988708888881498E-4 4.988708888881498E-4 0.0 3.502323075439256 3.3258059259209987E-4 50-51 4.988708888881498E-4 4.988708888881498E-4 0.0 4.1667359542901234 3.3258059259209987E-4 52-53 4.988708888881498E-4 4.988708888881498E-4 0.0 4.781677469992916 3.3258059259209987E-4 54-55 4.988708888881498E-4 4.988708888881498E-4 0.0 5.419899627177156 3.3258059259209987E-4 56-57 4.988708888881498E-4 4.988708888881498E-4 0.0 6.1317883856205455 3.3258059259209987E-4 58-59 4.988708888881498E-4 4.988708888881498E-4 0.0 6.904289957063845 3.3258059259209987E-4 60-61 4.988708888881498E-4 4.988708888881498E-4 0.0 7.780140947655141 3.3258059259209987E-4 62-63 4.988708888881498E-4 4.988708888881498E-4 0.0 8.62464621739463 3.3258059259209987E-4 64-65 4.988708888881498E-4 4.988708888881498E-4 0.0 9.463497617060053 3.3258059259209987E-4 66-67 6.651611851841997E-4 4.988708888881498E-4 0.0 10.366038200206866 3.3258059259209987E-4 68-69 6.651611851841997E-4 4.988708888881498E-4 0.0 11.369516993205378 3.3258059259209987E-4 70-71 6.651611851841997E-4 4.988708888881498E-4 0.0 12.46487117490746 3.3258059259209987E-4 72-73 6.651611851841997E-4 4.988708888881498E-4 0.0 13.5830071272021 3.3258059259209987E-4 74-75 6.651611851841997E-4 4.988708888881498E-4 0.0 14.692911709830085 3.3258059259209987E-4 76-77 6.651611851841997E-4 4.988708888881498E-4 0.0 15.82044306386545 3.3258059259209987E-4 78-79 6.651611851841997E-4 4.988708888881498E-4 0.0 17.03203416267847 3.3258059259209987E-4 80-81 6.651611851841997E-4 4.988708888881498E-4 0.0 18.344314035898748 3.3258059259209987E-4 82-83 6.651611851841997E-4 4.988708888881498E-4 0.0 19.663494956415313 3.3258059259209987E-4 84-85 6.651611851841997E-4 4.988708888881498E-4 0.0 20.99681055211704 3.3258059259209987E-4 86-87 6.651611851841997E-4 4.988708888881498E-4 0.0 22.313247682744723 4.1572574074012487E-4 88-89 6.651611851841997E-4 4.988708888881498E-4 0.0 23.667432710631605 4.988708888881498E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2455 0.0 26.587858 1 CCTGCGT 90 9.005883E-4 26.357016 8 GTACATG 1825 0.0 22.190664 1 GTATCAA 3065 0.0 22.073547 1 GTATTAG 180 5.027752E-6 21.175407 1 GTCTAAC 135 3.7159206E-4 21.175406 1 TACATGG 1855 0.0 20.716188 2 CGCAGAA 145 6.1915495E-4 19.631433 2 GAGTACT 2090 0.0 19.069437 12-13 AGACCGT 125 0.006070095 18.97705 6 AGTACTT 2195 0.0 18.913782 12-13 ACATGGG 1865 0.0 18.570038 3 CTATACG 65 0.007608919 18.250198 40-41 ATCAACG 3720 0.0 18.10982 3 GTCATAT 185 1.4848229E-4 18.02771 1 TCAACGC 3760 0.0 17.917162 4 TATCAAC 3840 0.0 17.790985 2 CAACGCA 3770 0.0 17.743793 5 GGGTAAG 215 2.3296256E-5 17.728249 1 AACGCAG 3850 0.0 17.375093 6 >>END_MODULE