FastQCFastQC Report
Thu 26 May 2016
SRR938842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599576
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.42963694344003095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.29470826050408955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.21431811813681667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.1045738988885479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31950.028.8429321
CCGCGTA350.00837659427.15288592-93
GTACATG16250.025.1393241
CAACGCA35550.024.8220025
AACGCAG35700.024.7115086
ATCAACG36150.024.6724933
TCAACGC36150.024.6724934
TATCAAC37600.024.0995542
TACATGG18200.023.4603562
ACATGGG17850.023.1230163
GTATAGC1452.3312956E-522.9318221
ACGCAGA39250.022.3556147
AGTACTT19450.022.31943112-13
CGCAGAG39700.022.1022158
GAGTACT18700.021.56547212-13
GCAGAGT40100.020.9355039
GGTATCA22550.020.4330751
ATACAGT3558.0035534E-1118.704856
TAGCCCT2803.5288394E-818.6379494
AGAGTAC33050.018.5884710-11
Produced by FastQC (version 0.11.5)