##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938842_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 599576 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.474473628030474 33.0 31.0 34.0 30.0 34.0 2 31.554932152054118 33.0 31.0 34.0 30.0 34.0 3 31.575081390849533 33.0 31.0 34.0 28.0 34.0 4 35.11813181314796 37.0 35.0 37.0 33.0 37.0 5 35.136271298384194 37.0 35.0 37.0 33.0 37.0 6 35.187937809385296 37.0 35.0 37.0 33.0 37.0 7 35.11684256874858 37.0 35.0 37.0 32.0 37.0 8 35.08187786035465 37.0 35.0 37.0 32.0 37.0 9 36.663637303694614 39.0 37.0 39.0 32.0 39.0 10-11 36.54917224838886 39.0 37.0 39.0 32.0 39.0 12-13 36.463354103566516 39.0 37.0 39.0 32.0 39.0 14-15 37.63356021588589 40.0 38.0 41.0 32.0 41.0 16-17 37.525121085567136 40.0 37.0 41.0 32.0 41.0 18-19 37.491335543784274 40.0 37.0 41.0 32.0 41.0 20-21 37.39295935794628 40.0 37.0 41.0 32.0 41.0 22-23 37.37615831854511 40.0 37.0 41.0 32.0 41.0 24-25 37.32872646670314 40.0 37.0 41.0 32.0 41.0 26-27 37.19792236513803 40.0 37.0 41.0 31.0 41.0 28-29 37.03118370315023 40.0 37.0 41.0 31.0 41.0 30-31 36.80586197579623 40.0 36.0 41.0 30.0 41.0 32-33 36.626760410690224 39.0 36.0 41.0 30.0 41.0 34-35 36.35492831600998 39.0 35.5 41.0 30.0 41.0 36-37 36.14545111879062 39.0 35.0 41.0 29.5 41.0 38-39 35.98380772412504 39.0 35.0 41.0 29.0 41.0 40-41 35.861261124528 39.0 35.0 41.0 28.0 41.0 42-43 35.856019086821355 39.0 35.0 41.0 28.0 41.0 44-45 35.68986417068061 39.0 35.0 41.0 27.0 41.0 46-47 35.39100881289444 39.0 35.0 41.0 25.5 41.0 48-49 35.11986970792694 39.0 35.0 41.0 24.0 41.0 50-51 34.02857018960065 38.0 34.0 40.0 23.0 40.5 52-53 34.10615751797937 38.0 33.5 39.5 23.0 40.5 54-55 34.62274840887561 39.0 34.5 40.0 23.0 41.0 56-57 34.54095894432065 39.0 34.0 40.0 23.0 41.0 58-59 34.38019533803888 38.0 34.0 40.0 22.5 41.0 60-61 34.107436755307084 38.0 34.0 40.0 21.5 41.0 62-63 33.75252678559515 38.0 33.5 40.0 19.5 41.0 64-65 33.39322871495857 37.0 33.0 40.0 18.0 41.0 66-67 33.03122373143688 37.0 33.0 40.0 15.5 41.0 68-69 32.61962620251644 36.0 32.0 39.0 11.5 41.0 70-71 32.195177091811544 36.0 32.0 39.0 7.5 41.0 72-73 31.749477964428195 35.0 31.0 39.0 4.5 40.0 74-75 31.250097568948725 35.0 31.0 38.0 2.0 40.0 76-77 30.73266858580063 35.0 31.0 37.0 2.0 39.0 78-79 30.23709838285722 35.0 30.0 37.0 2.0 39.0 80-81 29.681297283413613 34.5 29.5 36.0 2.0 39.0 82-83 29.184757228441434 34.0 29.0 36.0 2.0 37.5 84-85 28.74050078722297 34.0 29.0 35.0 2.0 37.0 86-87 28.20822631326137 34.0 28.5 35.0 2.0 37.0 88-89 27.648446568908696 34.0 26.5 35.0 2.0 36.0 90-91 27.09295485476403 34.0 26.0 35.0 2.0 36.0 92-93 26.535654362416107 33.5 25.0 35.0 2.0 35.5 94-95 25.932587361735628 33.0 23.0 35.0 2.0 35.0 96-97 25.261860881689728 33.0 18.5 35.0 2.0 35.0 98-99 24.453844049795187 32.5 2.0 35.0 2.0 35.0 100-101 22.11345350714505 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07697485178524488 1101 2 0.09287610758244824 1101 3 0.05162700682923216 1101 4 0.05340062290056835 1101 5 0.011538804953971749 1101 6 -0.04996035108931096 1101 7 0.08296671131972033 1101 8 0.07732582209700922 1101 9 0.07329779279627502 1101 10-11 -0.022248240655848406 1101 12-13 0.07705235607708971 1101 14-15 0.23425893040178636 1101 16-17 0.3208126268541349 1101 18-19 0.2643733622932274 1101 20-21 0.38699321957026456 1101 22-23 0.4755590987177243 1101 24-25 0.23208764688880734 1101 26-27 0.13448899210233378 1101 28-29 0.08310662019103887 1101 30-31 -0.06359174444369842 1101 32-33 -0.10045762634003097 1101 34-35 -0.12509345634595093 1101 36-37 -0.4017476071573256 1101 38-39 -0.2906136251588691 1101 40-41 -0.2859897126025359 1101 42-43 -0.35484526476756884 1101 44-45 -0.4826548831919837 1101 46-47 -0.39142000988121595 1101 48-49 -0.3639657680623145 1101 50-51 -0.49985620128159525 1101 52-53 -0.8324069687018039 1101 54-55 -0.8650021234985914 1101 56-57 -0.7766528968361825 1101 58-59 -0.848904124539537 1101 60-61 -0.8717855052496262 1101 62-63 -0.866754380846352 1101 64-65 -0.8903030880385003 1101 66-67 -1.237829301720943 1101 68-69 -1.0167633525393889 1101 70-71 -1.2952047258682953 1101 72-73 -1.2964493082701622 1101 74-75 -1.0797199913541888 1101 76-77 -1.4890749241107564 1101 78-79 -1.3943695405277694 1101 80-81 -1.369647217877958 1101 82-83 -1.2346991706261754 1101 84-85 -1.350094733024875 1101 86-87 -1.0477915245359988 1101 88-89 -1.0121967065616424 1101 90-91 -1.1027810226008192 1101 92-93 -1.3924986363885843 1101 94-95 -1.606619348984534 1101 96-97 -1.5352825724401669 1101 98-99 -1.4858926298906567 1101 100-101 -1.3109878962240984 1102 1 -0.030485183738733213 1102 2 0.1430938152990464 1102 3 0.17354641180259023 1102 4 0.3596746755944693 1102 5 0.17738829676372347 1102 6 0.2949891999270662 1102 7 0.1750176043530729 1102 8 0.18729905576584827 1102 9 0.10927815593027645 1102 10-11 0.3130339776192699 1102 12-13 0.2534804076855437 1102 14-15 0.3028430284874375 1102 16-17 0.5327261111643722 1102 18-19 0.3865379197236649 1102 20-21 0.41944739610470094 1102 22-23 0.2642423062369659 1102 24-25 0.3703274214102237 1102 26-27 0.38533854558566816 1102 28-29 0.5321627061393315 1102 30-31 0.5657716609243693 1102 32-33 0.36864545487172506 1102 34-35 0.484571409452343 1102 36-37 0.33164832485372386 1102 38-39 0.14158707298599182 1102 40-41 0.3737170912261618 1102 42-43 0.4241135619543499 1102 44-45 0.42862613861579746 1102 46-47 0.32104545291728925 1102 48-49 0.35294327575059015 1102 50-51 0.5612731035338854 1102 52-53 0.3115514879786758 1102 54-55 0.1779149748830946 1102 56-57 0.02524541577483319 1102 58-59 0.1995851812627123 1102 60-61 0.11516282462998362 1102 62-63 0.017547535968326144 1102 64-65 -0.22737692364142248 1102 66-67 -0.11814248013025264 1102 68-69 -0.12831080418730778 1102 70-71 -0.01492916833918656 1102 72-73 -0.07709182356520827 1102 74-75 -0.282641468559639 1102 76-77 0.0833098079792407 1102 78-79 -0.09555028110404606 1102 80-81 -0.01909153897525684 1102 82-83 -0.27997836689615596 1102 84-85 -0.39328319513262855 1102 86-87 -0.6789573401073383 1102 88-89 -0.47722316452493274 1102 90-91 -0.39898223880370054 1102 92-93 -0.2216011625381782 1102 94-95 -0.48476932678204676 1102 96-97 -0.9446147093061619 1102 98-99 -0.6364671192334619 1102 100-101 -0.26520606032514493 1103 1 0.17302358819109287 1103 2 0.217636943954016 1103 3 0.245092464434169 1103 4 0.20358914927867744 1103 5 0.33727499267015304 1103 6 0.20575308881595333 1103 7 0.30646862774488426 1103 8 0.3537646990406955 1103 9 0.4144682144098084 1103 10-11 0.2681509366835968 1103 12-13 0.32973223517092265 1103 14-15 0.36327797000790696 1103 16-17 0.5314651462520956 1103 18-19 0.588539016214888 1103 20-21 0.37239878499358525 1103 22-23 -0.0013004569209300598 1103 24-25 0.07552661731665467 1103 26-27 0.18150996371431916 1103 28-29 -0.01647581725248415 1103 30-31 0.19364081297115376 1103 32-33 0.14878982621675618 1103 34-35 0.1081276960020574 1103 36-37 0.10757120497068229 1103 38-39 2.950408222446299E-4 1103 40-41 -0.056308493569169116 1103 42-43 0.31455581341634087 1103 44-45 0.5499072058672567 1103 46-47 0.35752206110441875 1103 48-49 0.38611213539972056 1103 50-51 0.3414357497327103 1103 52-53 0.18560119558101462 1103 54-55 0.0252194339474201 1103 56-57 -0.08012739124271206 1103 58-59 -0.11266810821096129 1103 60-61 -0.37645815490217416 1103 62-63 -0.2348373324527202 1103 64-65 -0.3264814558051725 1103 66-67 -0.37283911755715593 1103 68-69 -0.24959187143876704 1103 70-71 -0.5860183496257392 1103 72-73 -0.35536302239561834 1103 74-75 -0.4671261176824437 1103 76-77 -0.35893251658216485 1103 78-79 -0.39155722554848893 1103 80-81 -0.4797092290337339 1103 82-83 -0.47951235812422865 1103 84-85 -0.30915490565894643 1103 86-87 -0.48792115589681373 1103 88-89 -0.22933391013897264 1103 90-91 -0.13055204874522275 1103 92-93 -0.36158654265513945 1103 94-95 -0.3445006864311715 1103 96-97 -0.108147238545925 1103 98-99 -0.2431283034439886 1103 100-101 -0.11009823868771562 1104 1 0.39783649458294335 1104 2 0.35462013620036714 1104 3 0.24380427893545686 1104 4 0.5817390695338602 1104 5 0.5347722322726582 1104 6 0.5503917834546499 1104 7 0.7297111746716425 1104 8 0.5513846229764141 1104 9 0.5643577985099242 1104 10-11 0.5964450110303048 1104 12-13 0.4177735797912163 1104 14-15 0.42778232530174165 1104 16-17 0.57384644915971 1104 18-19 0.6466085782318203 1104 20-21 0.6247880623828692 1104 22-23 0.5138234542556148 1104 24-25 0.3370681459634426 1104 26-27 0.47195592126553265 1104 28-29 0.26033991087903274 1104 30-31 0.35876165703936636 1104 32-33 0.4536481258118954 1104 34-35 0.8099526254585641 1104 36-37 0.630921100689001 1104 38-39 0.6581529723643911 1104 40-41 0.7998655954996607 1104 42-43 0.7980087639745577 1104 44-45 0.7953280131301739 1104 46-47 0.942014761386595 1104 48-49 0.977542634724955 1104 50-51 1.144831038654317 1104 52-53 1.1448362587634477 1104 54-55 0.9701218999025158 1104 56-57 1.0831562549356732 1104 58-59 1.0644052570202902 1104 60-61 1.2999251109547671 1104 62-63 1.1546578215161176 1104 64-65 1.1747331190936237 1104 66-67 1.233049730436953 1104 68-69 1.0154768317878293 1104 70-71 0.8294521447921284 1104 72-73 0.8624599441115599 1104 74-75 0.57440252910936 1104 76-77 0.8055381005200317 1104 78-79 0.6795383455470834 1104 80-81 0.6137148907061736 1104 82-83 0.28530790932762073 1104 84-85 0.6139465542846203 1104 86-87 0.14055387784138063 1104 88-89 0.4150226105333381 1104 90-91 0.21291194340298247 1104 92-93 0.5502034217664047 1104 94-95 0.6655256907004734 1104 96-97 0.45140680390285226 1104 98-99 0.4648768492980082 1104 100-101 1.0575345671029837 1105 1 -0.11409722387251264 1105 2 -0.05500284950957379 1105 3 0.12750753220392852 1105 4 0.10028574025444215 1105 5 0.14088351229698048 1105 6 0.1670685849140625 1105 7 0.07457283475068976 1105 8 0.010186690474128568 1105 9 -0.02647528145359246 1105 10-11 -0.03790433527556303 1105 12-13 0.15169726480185375 1105 14-15 0.30817910243764146 1105 16-17 0.13495924940294657 1105 18-19 0.25914278315732986 1105 20-21 0.2915180715041785 1105 22-23 0.2162786077977188 1105 24-25 0.0703837434094794 1105 26-27 0.10106051232665436 1105 28-29 0.45800637484985174 1105 30-31 0.32622897233277115 1105 32-33 0.44695916260118906 1105 34-35 0.22387022959355818 1105 36-37 0.39768151418854103 1105 38-39 0.4720781775939429 1105 40-41 0.23088566238929076 1105 42-43 0.31704950584138913 1105 44-45 0.13490691249167952 1105 46-47 0.25822029498343113 1105 48-49 0.3195158788721102 1105 50-51 0.6292107893384085 1105 52-53 0.5560934798032733 1105 54-55 0.5725086435936149 1105 56-57 0.16916263985510227 1105 58-59 0.3647625084645014 1105 60-61 0.6703264487474101 1105 62-63 0.7050910839059057 1105 64-65 0.4757364635752239 1105 66-67 0.49414732849107423 1105 68-69 0.3702579202645637 1105 70-71 0.2443958966923958 1105 72-73 0.2923398468175442 1105 74-75 0.496187266111491 1105 76-77 0.5600987572546003 1105 78-79 0.15833129173133287 1105 80-81 0.14845189283448335 1105 82-83 0.4572446682208984 1105 84-85 0.43197635057528316 1105 86-87 0.4532629079417134 1105 88-89 0.6661627747169838 1105 90-91 0.8359356588179985 1105 92-93 0.7807446196989076 1105 94-95 0.6827421698734746 1105 96-97 0.7119343198651507 1105 98-99 0.6986865178902839 1105 100-101 0.8872320435395871 1106 1 0.25405428994547563 1106 2 0.1366062421996297 1106 3 0.25989509601311767 1106 4 0.26894002647166104 1106 5 0.2310249926701573 1106 6 0.46485396600893836 1106 7 0.4804817856396184 1106 8 0.40595768149683664 1106 9 0.2746655828308633 1106 10-11 0.49331541036780635 1106 12-13 0.38702390183758695 1106 14-15 0.4159643735166796 1106 16-17 0.6064651462520985 1106 18-19 0.3411705951622537 1106 20-21 0.35589659201112767 1106 22-23 0.4302236658860892 1106 24-25 0.3385748629306846 1106 26-27 0.28194856020554937 1106 28-29 0.25397374415102547 1106 30-31 0.0867877427957211 1106 32-33 0.19599377358517245 1106 34-35 0.39337988898451215 1106 36-37 0.4978124330408562 1106 38-39 0.39437398819067226 1106 40-41 0.48464545379925283 1106 42-43 0.48341546253914913 1106 44-45 0.5930541356918155 1106 46-47 0.6753948681219626 1106 48-49 0.7548073108383164 1106 50-51 0.6091989076274515 1106 52-53 0.2019937394406668 1106 54-55 0.46387074973689124 1106 56-57 0.7990940999853535 1106 58-59 0.7375511900346439 1106 60-61 0.6653729854486983 1106 62-63 0.5135727552665728 1106 64-65 0.3259856494579907 1106 66-67 0.4179503561270579 1106 68-69 0.5329190934735095 1106 70-71 0.5600891065146101 1106 72-73 0.5620711881306981 1106 74-75 0.7571063384579091 1106 76-77 0.4263745009616926 1106 78-79 0.4960524235743158 1106 80-81 0.36294427973819765 1106 82-83 0.28951176468278916 1106 84-85 0.6106915855691248 1106 86-87 0.5692608616470487 1106 88-89 0.27538100214173156 1106 90-91 0.29998523195652993 1106 92-93 0.0501459134852098 1106 94-95 -0.18419366888730693 1106 96-97 -0.07898057187926355 1106 98-99 -0.07273356660188313 1106 100-101 0.2662723753473699 1107 1 0.2379877226652063 1107 2 0.19285948306040268 1107 3 0.2633709191917539 1107 4 0.43082102206408734 1107 5 0.3567892956690315 1107 6 0.061418113446499945 1107 7 0.18881241825564388 1107 8 0.33447509572205547 1107 9 0.07435067919245597 1107 10-11 0.16148772693508562 1107 12-13 0.25011508885685885 1107 14-15 0.20561225399372773 1107 16-17 0.36146687471117644 1107 18-19 0.3741841635660279 1107 20-21 0.35047760272757955 1107 22-23 0.34507410016142614 1107 24-25 0.2585877183450833 1107 26-27 0.27614741942255705 1107 28-29 0.4496204579182006 1107 30-31 0.42682290613510077 1107 32-33 0.220036909442058 1107 34-35 0.2147044827966269 1107 36-37 0.07792381062274245 1107 38-39 0.25562733708013496 1107 40-41 0.043060618867095 1107 42-43 0.39080246452687817 1107 44-45 0.6942810715659888 1107 46-47 0.875472108637048 1107 48-49 0.724479605799857 1107 50-51 0.8608463451868644 1107 52-53 0.720179019451038 1107 54-55 0.693433071489558 1107 56-57 0.7614420496007739 1107 58-59 0.7396739538407147 1107 60-61 0.996495781663377 1107 62-63 0.8816305614198896 1107 64-65 0.8451890998944265 1107 66-67 0.8916669752610957 1107 68-69 0.8979061840447713 1107 70-71 0.9470816417319696 1107 72-73 1.0434011293630867 1107 74-75 0.9236136628032554 1107 76-77 0.627939836714873 1107 78-79 0.7081964690327922 1107 80-81 0.5926267834111698 1107 82-83 0.5029264976358654 1107 84-85 0.7290613245718021 1107 86-87 0.7083194456069464 1107 88-89 0.7081088346540447 1107 90-91 0.8386554527671812 1107 92-93 0.8421987827272801 1107 94-95 0.8883962109847836 1107 96-97 0.9386767178969038 1107 98-99 1.254966070421709 1107 100-101 1.8057906213935233 1108 1 -0.15416009539951148 1108 2 0.03263911368931005 1108 3 -0.11449466593592561 1108 4 0.09121545886890914 1108 5 0.06530177340290777 1108 6 0.07984025018227214 1108 7 -0.13732902894100363 1108 8 0.055041743241304175 1108 9 0.019638383649166258 1108 10-11 0.3033657404775383 1108 12-13 0.04400909339421588 1108 14-15 0.043855739520026304 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-1.3929007237354867 2316 94-95 -1.5058707901106807 2316 96-97 -1.411727769563175 2316 98-99 -1.6700862130275382 2316 100-101 -2.2087236809958846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5705.0 3 1041.0 4 869.0 5 851.0 6 904.0 7 1014.0 8 1166.0 9 1313.0 10 1584.0 11 1929.0 12 2538.0 13 3138.0 14 3459.0 15 3850.0 16 4534.0 17 5710.0 18 5200.0 19 3893.0 20 3922.0 21 4182.0 22 4721.0 23 5471.0 24 6090.0 25 7165.0 26 8383.0 27 9634.0 28 11183.0 29 13108.0 30 15810.0 31 18747.0 32 22314.0 33 28048.0 34 35348.0 35 48610.0 36 68787.0 37 103647.0 38 111319.0 39 24337.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39977723442899 21.46827931992949 14.4682224578468 22.663720987794722 2 14.966879672555653 22.421584596750616 40.46727644823122 22.144259282462514 3 17.807182164408697 26.64108730128226 31.926189716226126 23.625540818082918 4 12.487533348813978 18.249005591407908 39.350681677322136 29.91277938245598 5 12.812098253169255 37.52869172424516 35.41407194287902 14.245138079706571 6 28.489686647203623 38.12355969846206 19.01858124762909 14.368172406705227 7 24.92986662119282 34.31854430189285 23.628512102396403 17.12307697451793 8 23.510872727394325 35.505482342476704 22.600660088078623 18.382984842050355 9 24.963920143250395 17.39232835342224 23.145562177619798 34.49818932570757 10-11 22.701478156887354 27.336417945120427 29.99666581153228 19.965438086459937 12-13 23.954241074226633 25.600964049542917 29.84391857391534 20.600876302315104 14-15 21.418702059869716 27.18538493282743 28.118416194261638 23.277496813041218 16-17 19.96609749937698 30.171803890675726 29.067589242855302 20.794509367091997 18-19 19.96800331668859 28.746445509514583 31.4558825250298 19.829668648767022 20-21 20.978809452187573 28.439369631337946 30.712757779337547 19.869063137136937 22-23 20.61420631092512 28.443552020785074 30.685989385099848 20.256252283189955 24-25 20.543765735703705 28.212674827220717 30.972880256519137 20.27067918055644 26-27 20.308967280796736 28.331790895657022 30.943001209962866 20.41624061358338 28-29 20.23728561187248 28.52312585272164 30.980553554892477 20.259034980513402 30-31 20.503440991326645 28.62550188963906 30.59651376438346 20.274543354650838 32-33 20.041225467546433 28.671335098547583 30.794271214112577 20.493168219793404 34-35 20.56694616170653 28.35492382999984 30.67220244825269 20.40592756004094 36-37 20.805862461875662 28.33512077136951 30.337135092641198 20.521881674113626 38-39 20.631543174172425 28.300580666816778 30.549252047233484 20.518624111777317 40-41 20.683425696826284 28.017642082609196 30.81046977776699 20.48846244279753 42-43 20.166884151195134 28.340005741926355 30.668666575710983 20.824443531167528 44-45 20.303054146946607 28.343565163673013 30.38610859523973 20.96727209414065 46-47 20.511058279897536 28.342681109678715 29.98191833112893 21.164342279294814 48-49 20.384348418740835 28.328196574992067 30.003524980051626 21.28393002621547 50-51 20.46979809582495 28.58640286396501 29.71388815399505 21.22991088621499 52-53 20.16098176438626 28.503685049073983 29.99026306469829 21.345070121841463 54-55 20.120771005582288 28.347448120205076 29.896558537018247 21.63522233719439 56-57 20.208799965180475 28.518529678622485 29.571066984060025 21.701603372137015 58-59 20.21404255746463 28.040930680939518 29.729890309256263 22.01513645233959 60-61 20.204750348626032 27.87008943397521 29.884047859850245 22.041112357548517 62-63 20.340254269919875 27.670353145225825 30.0015234766071 21.9878691082472 64-65 20.254051723055152 27.619960728326266 29.862549141926486 22.263438406692092 66-67 20.151604729022456 27.806705645316615 29.563005900391744 22.478683725269185 68-69 20.20328059404763 27.686491981914607 29.69760818281017 22.41261924122759 70-71 20.238517942860206 27.425125028891472 29.579256425630838 22.757100602617484 72-73 20.051498480166803 27.463009018514335 29.47588782542434 23.009604675894522 74-75 19.9535615121564 27.446603274976805 29.48869098058392 23.11114423228287 76-77 19.948109965348042 27.149254593540622 29.862472152431792 23.04016328867955 78-79 19.923380947518375 26.98214342510768 29.920267304572874 23.174208322801075 80-81 20.049672782429194 26.99574257210255 29.67860855938886 23.2759760860794 82-83 19.85463458983497 26.90281429912089 29.810624801696434 23.431926309347705 84-85 19.812056039949603 26.534840791792412 29.766472304427356 23.886630863830625 86-87 19.907700009124525 26.55997950749341 29.717483004529615 23.814837478852454 88-89 20.061333052035142 26.11502082108933 29.829998777692385 23.99364734918315 90-91 20.05923591200193 25.90779830649267 30.031116857364882 24.001848924140518 92-93 20.178007253263843 25.925268438588837 29.771960131332477 24.124764176814846 94-95 20.114561830309476 25.62215532004874 29.712548623876263 24.550734225765524 96-97 20.235975700649693 25.194078247659757 29.758078428548217 24.811867623142334 98-99 20.301654148686637 24.991186192007863 29.71212135169062 24.995038307614877 100-101 20.710334111374923 24.436477983209038 30.061400541838434 24.79178736357761 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 895.0 1 596.5 2 335.5 3 368.0 4 325.0 5 335.5 6 474.0 7 717.5 8 1019.0 9 1103.0 10 877.0 11 735.0 12 809.0 13 819.5 14 810.5 15 861.5 16 885.0 17 942.0 18 1044.5 19 1177.5 20 1360.5 21 1586.0 22 1935.5 23 2360.5 24 2847.5 25 3551.5 26 4625.0 27 5878.0 28 7048.5 29 8306.0 30 9634.0 31 11135.5 32 12962.0 33 14772.0 34 16368.0 35 18239.0 36 20273.0 37 21817.0 38 22266.0 39 22022.5 40 22057.0 41 22112.5 42 21807.0 43 21567.0 44 21443.0 45 21135.5 46 20729.5 47 20642.0 48 21338.5 49 20507.5 50 18364.5 51 16821.5 52 15568.0 53 14642.5 54 13749.0 55 12546.0 56 11235.0 57 9923.5 58 8814.0 59 7871.0 60 6618.0 61 5376.0 62 4377.0 63 3446.0 64 2613.0 65 2016.0 66 1601.0 67 1342.5 68 1094.0 69 854.5 70 676.5 71 560.0 72 462.0 73 384.0 74 329.0 75 280.5 76 220.0 77 157.5 78 137.0 79 106.5 80 80.0 81 76.0 82 57.5 83 42.5 84 31.5 85 22.5 86 16.5 87 10.5 88 5.5 89 7.5 90 8.0 91 4.0 92 2.0 93 3.0 94 3.0 95 1.5 96 0.0 97 1.5 98 3.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27319305642654146 2 0.1669513122606642 3 0.1963053891416601 4 0.16328205265053972 5 0.23466583052023432 6 0.1963053891416601 7 0.23850187465809172 8 0.2458403938783407 9 0.15077321307056987 10-11 0.205061576847639 12-13 0.21123260437375746 14-15 0.17395626242544732 16-17 0.2810319292299892 18-19 0.23166370902104155 20-21 0.1994742951685858 22-23 0.2425880955875485 24-25 0.23583332221436484 26-27 0.339740082991981 28-29 0.3098022602639198 30-31 0.2852849346871789 32-33 0.1933866599063338 34-35 0.35516765180727716 36-37 0.39236060149172086 38-39 0.37451465702429715 40-41 0.33815562997851817 42-43 0.3695111211923092 44-45 0.37643267909322586 46-47 0.3812694303974809 48-49 0.40186731957249794 50-51 0.3939450545051837 52-53 0.39452880035224897 54-55 0.3886079496177299 56-57 0.3685938062897781 58-59 0.3465782486290312 60-61 0.3123040281799138 62-63 0.3765994636209588 64-65 0.40920583879274686 66-67 0.4164609657491294 68-69 0.4202136176231204 70-71 0.4192963027205892 72-73 0.4121245680280732 74-75 0.4072878167238182 76-77 0.3921938169639879 78-79 0.36775988365111345 80-81 0.3781005243705552 82-83 0.3873570656597329 84-85 0.3875238501874658 86-87 0.3810192536058815 88-89 0.3911097175337238 90-91 0.4122913525558061 92-93 0.411207253125542 94-95 0.42004683309538743 96-97 0.42079736347018554 98-99 0.4172114961239276 100-101 0.39194364017238853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 599576.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.07512042833012 #Duplication Level Percentage of deduplicated Percentage of total 1 91.63907546448087 76.1292723015258 2 6.037770224897045 10.031769771038158 3 1.0245391352765059 2.553411361398988 4 0.40769328057441623 1.3547667352616244 5 0.2197490681457326 0.9127840150110031 6 0.13694998167117275 0.6826281731994168 7 0.1004771039574398 0.5843003258087911 8 0.07504405723261877 0.49874352736242433 9 0.05304657930479333 0.39661658676510037 >10 0.2867924314111922 4.1247710852364445 >50 0.012642040438945118 0.7126609314190813 >100 0.005217304769143815 0.8801926969681455 >500 4.0133113605612166E-4 0.1998770915947076 >1k 6.019967040841825E-4 0.938205397410354 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2576 0.42963694344003095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.29470826050408955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1285 0.21431811813681667 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 627 0.1045738988885479 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.6678452773293128E-4 0.0 0.0 0.0 3 0.0 1.6678452773293128E-4 0.0 0.0 0.0 4 0.0 1.6678452773293128E-4 0.0 0.0 0.0 5 0.0 1.6678452773293128E-4 0.0 0.0 0.0 6 0.0 1.6678452773293128E-4 0.0 0.0 0.0 7 0.0 1.6678452773293128E-4 0.0 0.0 0.0 8 0.0 1.6678452773293128E-4 0.0 0.0 0.0 9 0.0 1.6678452773293128E-4 0.0 1.6678452773293128E-4 0.0 10-11 0.0 1.6678452773293128E-4 0.0 1.6678452773293128E-4 0.0 12-13 0.0 1.6678452773293128E-4 0.0 1.6678452773293128E-4 0.0 14-15 0.0 1.6678452773293128E-4 0.0 1.6678452773293128E-4 1.6678452773293128E-4 16-17 0.0 1.6678452773293128E-4 0.0 1.6678452773293128E-4 1.6678452773293128E-4 18-19 0.0 1.6678452773293128E-4 0.0 1.6678452773293128E-4 1.6678452773293128E-4 20-21 0.0 1.6678452773293128E-4 0.0 3.335690554658625E-4 1.6678452773293128E-4 22-23 0.0 1.6678452773293128E-4 0.0 0.0010007071663975878 1.6678452773293128E-4 24-25 1.6678452773293128E-4 1.6678452773293128E-4 0.0 0.002751944707593366 1.6678452773293128E-4 26-27 1.6678452773293128E-4 1.6678452773293128E-4 0.0 0.007421911484115442 1.6678452773293128E-4 28-29 1.6678452773293128E-4 1.6678452773293128E-4 0.0 0.026268563117936676 1.6678452773293128E-4 30-31 1.6678452773293128E-4 4.1696131933232814E-4 0.0 0.086144208574059 1.6678452773293128E-4 32-33 1.6678452773293128E-4 5.003535831987938E-4 0.0 0.19255273726766914 1.6678452773293128E-4 34-35 1.6678452773293128E-4 5.003535831987938E-4 0.0 0.34766234805929525 1.6678452773293128E-4 36-37 1.6678452773293128E-4 5.003535831987938E-4 0.0 0.5975055706032263 1.6678452773293128E-4 38-39 1.6678452773293128E-4 5.003535831987938E-4 0.0 0.9907834869974783 1.6678452773293128E-4 40-41 1.6678452773293128E-4 5.003535831987938E-4 0.0 1.4347638998225412 1.6678452773293128E-4 42-43 1.6678452773293128E-4 5.837458470652594E-4 0.0 1.834629805062244 1.6678452773293128E-4 44-45 1.6678452773293128E-4 6.671381109317251E-4 0.0 2.3169706592658814 1.6678452773293128E-4 46-47 1.6678452773293128E-4 6.671381109317251E-4 0.0 2.8666924626736225 1.6678452773293128E-4 48-49 1.6678452773293128E-4 6.671381109317251E-4 0.0 3.4531902544464756 1.6678452773293128E-4 50-51 1.6678452773293128E-4 6.671381109317251E-4 0.0 4.0979792386619875 3.3356905546586257E-4 52-53 1.6678452773293128E-4 6.671381109317251E-4 0.0 4.704491173762793 5.003535831987938E-4 54-55 1.6678452773293128E-4 6.671381109317251E-4 0.0 5.329849760497418 5.003535831987938E-4 56-57 1.6678452773293128E-4 6.671381109317251E-4 0.0 6.0153341694797655 6.67138110931725E-4 58-59 1.6678452773293128E-4 6.671381109317251E-4 0.0 6.774370555192336 8.339226386646564E-4 60-61 1.6678452773293128E-4 6.671381109317251E-4 0.0 7.61046139271752 8.339226386646564E-4 62-63 1.6678452773293128E-4 6.671381109317251E-4 0.0 8.446135268923372 8.339226386646564E-4 64-65 1.6678452773293128E-4 6.671381109317251E-4 0.0 9.284310913045218 8.339226386646564E-4 66-67 1.6678452773293128E-4 6.671381109317251E-4 0.0 10.192869627870362 8.339226386646564E-4 68-69 1.6678452773293128E-4 6.671381109317251E-4 0.0 11.181818485062777 8.339226386646564E-4 70-71 1.6678452773293128E-4 6.671381109317251E-4 0.0 12.258662788370447 8.339226386646564E-4 72-73 1.6678452773293128E-4 6.671381109317251E-4 0.0 13.357272472547267 9.173149025311219E-4 74-75 1.6678452773293128E-4 6.671381109317251E-4 0.0 14.45980159312581 0.0010007071663975875 76-77 1.6678452773293128E-4 6.671381109317251E-4 0.0 15.565833188786742 0.0010007071663975875 78-79 1.6678452773293128E-4 6.671381109317251E-4 0.0 16.767932672421843 0.0010007071663975875 80-81 1.6678452773293128E-4 6.671381109317251E-4 0.0 18.024654088889484 0.0010007071663975875 82-83 1.6678452773293128E-4 6.671381109317251E-4 0.0 19.292133107395895 0.0010007071663975875 84-85 2.5017679159939694E-4 6.671381109317251E-4 0.0 20.585797296756375 0.0010007071663975875 86-87 3.3356905546586257E-4 6.671381109317251E-4 0.0 21.887300358920303 0.0010007071663975875 88-89 3.3356905546586257E-4 6.671381109317251E-4 0.0 23.226580116615743 0.0010007071663975875 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3195 0.0 28.842932 1 CCGCGTA 35 0.008376594 27.152885 92-93 GTACATG 1625 0.0 25.139324 1 CAACGCA 3555 0.0 24.822002 5 AACGCAG 3570 0.0 24.711508 6 ATCAACG 3615 0.0 24.672493 3 TCAACGC 3615 0.0 24.672493 4 TATCAAC 3760 0.0 24.099554 2 TACATGG 1820 0.0 23.460356 2 ACATGGG 1785 0.0 23.123016 3 GTATAGC 145 2.3312956E-5 22.931822 1 ACGCAGA 3925 0.0 22.355614 7 AGTACTT 1945 0.0 22.319431 12-13 CGCAGAG 3970 0.0 22.102215 8 GAGTACT 1870 0.0 21.565472 12-13 GCAGAGT 4010 0.0 20.935503 9 GGTATCA 2255 0.0 20.433075 1 ATACAGT 355 8.0035534E-11 18.70485 6 TAGCCCT 280 3.5288394E-8 18.637949 4 AGAGTAC 3305 0.0 18.58847 10-11 >>END_MODULE