FastQCFastQC Report
Thu 26 May 2016
SRR938842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599576
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT25680.42830266721816757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.3824369220916114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.32773159699520993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.2164863169973448No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT12400.20681281438883475TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG7450.1242544731610338TruSeq Adapter, Index 3 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6870.11458097055252378No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT6560.10941065019280291RNA PCR Primer, Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.027.197521
GTATCAA34550.025.1850221
GTGTCCG1050.002210725322.5912889
GAGTACT22600.022.57006512-13
AGTACTT23350.021.3370912-13
GTAAGGT1605.0283124E-520.7574773
ATCAACG41500.020.5788423
TATCAAC42250.020.3258342
TCAACGC42100.020.2855574
GTACTTT25200.020.24140514-15
CAACGCA42300.020.077485
AACGCAG42550.019.9578516
GTACATG17400.019.1288171
ACGCAGA45650.018.3947037
TACATGG17900.018.2891122
CGCAGAG45800.018.2308758
AGAGTAC39800.018.00224310-11
ACATGGG18100.017.824893
TAGATAG1600.001199283517.7921245
GCAGAGT47650.017.6226279
Produced by FastQC (version 0.11.5)