##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938841_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 394835 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820947990932922 31.0 31.0 34.0 30.0 34.0 2 31.993468157584815 33.0 31.0 34.0 30.0 34.0 3 32.02782681373232 33.0 31.0 34.0 30.0 34.0 4 35.72275254220117 37.0 35.0 37.0 33.0 37.0 5 35.65938936517786 37.0 35.0 37.0 33.0 37.0 6 35.73490951916624 37.0 35.0 37.0 33.0 37.0 7 35.666990008484554 37.0 35.0 37.0 33.0 37.0 8 35.67618118961085 37.0 35.0 37.0 33.0 37.0 9 37.220563019995694 39.0 37.0 39.0 33.0 39.0 10-11 37.165225980472854 39.0 37.0 39.0 33.0 39.0 12-13 37.0834247217192 39.0 37.0 39.0 33.0 39.0 14-15 38.06368609672395 40.0 37.0 41.0 33.0 41.0 16-17 38.00621145541808 40.0 37.0 41.0 33.0 41.0 18-19 38.10143097749693 40.0 37.0 41.0 33.0 41.0 20-21 38.17046741043727 40.0 37.0 41.0 33.0 41.0 22-23 38.06664809350741 40.0 37.0 41.0 33.0 41.0 24-25 37.913876682664906 40.0 36.5 41.0 33.0 41.0 26-27 37.57801360061798 40.0 36.0 41.0 32.5 41.0 28-29 37.29122544860511 39.0 35.5 41.0 32.0 41.0 30-31 36.87824154393607 39.0 35.0 41.0 31.5 41.0 32-33 36.39395570301518 38.0 35.0 41.0 31.0 41.0 34-35 36.045234085124164 38.0 35.0 41.0 30.0 41.0 36-37 35.7053655324376 38.0 35.0 41.0 30.0 41.0 38-39 35.34605848012461 38.0 35.0 40.5 28.0 41.0 40-41 35.013983056213355 38.0 35.0 40.0 26.5 41.0 42-43 34.62726835260299 38.0 34.5 40.0 24.5 41.0 44-45 34.06591360948244 38.0 33.5 40.0 21.0 41.0 46-47 33.333196145225216 38.0 33.0 40.0 11.5 41.0 48-49 32.705731508098324 37.5 33.0 40.0 2.0 41.0 50-51 32.262599566907696 37.0 33.0 40.0 2.0 41.0 52-53 31.92126457887472 37.0 32.0 40.0 2.0 41.0 54-55 31.61755923360391 36.5 32.0 40.0 2.0 41.0 56-57 31.249566274519736 36.0 31.0 40.0 2.0 41.0 58-59 30.832963136500055 36.0 31.0 40.0 2.0 41.0 60-61 30.324213912140515 35.0 29.5 39.5 2.0 41.0 62-63 29.805525092760266 35.0 28.5 39.0 2.0 40.5 64-65 29.284546709384934 35.0 28.0 39.0 2.0 40.0 66-67 28.71579140653691 35.0 26.5 38.0 2.0 40.0 68-69 28.186400648372107 35.0 26.0 37.0 2.0 39.5 70-71 27.642449377588107 34.5 24.5 37.0 2.0 39.0 72-73 27.27579241961832 34.0 24.5 36.0 2.0 39.0 74-75 26.862456469158005 34.0 23.0 36.0 2.0 38.5 76-77 24.969568300682564 31.5 19.5 34.5 2.0 36.0 78-79 25.811456684437804 33.0 19.5 35.0 2.0 37.0 80-81 25.80299112287411 33.0 18.0 35.0 2.0 36.0 82-83 25.491438195702003 33.0 13.0 35.0 2.0 36.0 84-85 25.115363379639597 33.0 4.5 35.0 2.0 35.5 86-87 24.609122798130862 33.0 2.0 35.0 2.0 35.0 88-89 24.088064634594197 32.5 2.0 35.0 2.0 35.0 90-91 23.650279230564664 32.0 2.0 35.0 2.0 35.0 92-93 23.372161789101778 32.0 2.0 35.0 2.0 35.0 94-95 23.01796066711411 32.0 2.0 35.0 2.0 35.0 96-97 22.525204199222458 31.0 2.0 34.0 2.0 35.0 98-99 21.885657299884762 31.0 2.0 34.0 2.0 35.0 100-101 19.487879747236185 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1858427581134805 1101 2 0.2838708054571697 1101 3 0.023580202542810014 1101 4 0.13397798057444277 1101 5 0.214354127365894 1101 6 0.24725390225972887 1101 7 0.17653650860833636 1101 8 0.22023944055112565 1101 9 0.373689037225013 1101 10-11 0.2203615240710448 1101 12-13 0.20842973265449416 1101 14-15 0.39357632935659836 1101 16-17 0.3502084772444434 1101 18-19 0.3628635324662497 1101 20-21 0.4336102807336104 1101 22-23 0.48370910280255686 1101 24-25 0.511945112354347 1101 26-27 0.5374932174582128 1101 28-29 0.4200028352150653 1101 30-31 0.45504931785808367 1101 32-33 0.272154464924931 1101 34-35 0.3614427783657206 1101 36-37 0.24246187463818103 1101 38-39 0.1343971268153581 1101 40-41 0.3441085762087681 1101 42-43 0.3156416868766527 1101 44-45 -0.02137139975572211 1101 46-47 -0.572411080849939 1101 48-49 -0.5734450234610549 1101 50-51 -0.30189364698162535 1101 52-53 -0.3247578879134423 1101 54-55 -0.4732233279571112 1101 56-57 -0.38581863214795575 1101 58-59 -0.20890578994628584 1101 60-61 -0.6248668873103753 1101 62-63 -0.5821582885553411 1101 64-65 -0.7495965253684247 1101 66-67 -0.8094710709994217 1101 68-69 -0.9735948163818797 1101 70-71 -0.8209350147724983 1101 72-73 -0.806497830812404 1101 74-75 -0.41003584626740164 1101 76-77 -0.5425961424508259 1101 78-79 -0.574719811268313 1101 80-81 -0.19449072703572767 1101 82-83 -0.2709551213812631 1101 84-85 -0.2873013228457033 1101 86-87 -0.25282047332805035 1101 88-89 -0.4599100988954099 1101 90-91 -0.13552067233426257 1101 92-93 -0.33428064765709564 1101 94-95 -0.3717797815766346 1101 96-97 -0.4425295918483876 1101 98-99 -0.4270338249916854 1101 100-101 -0.14810978013092324 1102 1 -0.0014590688531512797 1102 2 0.11760174309871729 1102 3 0.16401505079888778 1102 4 0.1247223562350186 1102 5 0.08888228681025367 1102 6 9.455827670521444E-4 1102 7 0.23502963860984494 1102 8 0.03340567941569361 1102 9 0.28312584833780363 1102 10-11 0.08136862055300043 1102 12-13 0.2869741989084602 1102 14-15 0.4198861587390539 1102 16-17 0.27256994485731667 1102 18-19 0.3765582327531263 1102 20-21 0.3030425621770618 1102 22-23 0.31963693007116234 1102 24-25 0.008434618620384526 1102 26-27 0.030298592665822355 1102 28-29 0.06391797940954547 1102 30-31 0.09880592362586071 1102 32-33 0.3051757543708007 1102 34-35 0.05387823057770902 1102 36-37 0.43110750350727045 1102 38-39 0.49101044404319794 1102 40-41 0.1127708138654242 1102 42-43 0.14613391094429318 1102 44-45 -0.0808988042551988 1102 46-47 -0.11157007224357329 1102 48-49 0.06193169403856302 1102 50-51 0.1850005097449383 1102 52-53 -0.07068118551271851 1102 54-55 -0.11266919840856815 1102 56-57 -0.018998460020519303 1102 58-59 0.05151244952223166 1102 60-61 0.15594241361065642 1102 62-63 0.1796415000600895 1102 64-65 0.29480933300391143 1102 66-67 0.5929749504410147 1102 68-69 0.39771727783524113 1102 70-71 0.4407590815584683 1102 72-73 0.15670615529661092 1102 74-75 0.44712858072943007 1102 76-77 0.2506999469346489 1102 78-79 0.22973436357692378 1102 80-81 0.44012349612598456 1102 82-83 0.6562983891124716 1102 84-85 0.9408807698615256 1102 86-87 0.7708470489428265 1102 88-89 0.8374022972996684 1102 90-91 0.7664497338260894 1102 92-93 0.24390144505013467 1102 94-95 0.1965653792266302 1102 96-97 0.39091446673533525 1102 98-99 0.44575796120792077 1102 100-101 0.07586726954444956 1103 1 -0.14291807660742606 1103 2 0.1159229054867339 1103 3 0.020553483381888782 1103 4 -0.0021465739765318403 1103 5 0.06430199621325272 1103 6 0.055022222080459926 1103 7 0.17691776629185085 1103 8 0.17587349542111497 1103 9 0.020971214595427057 1103 10-11 -0.02172932418839224 1103 12-13 0.005565631566746276 1103 14-15 0.0872241095031896 1103 16-17 -0.0032562992140583447 1103 18-19 0.1121827151742707 1103 20-21 0.0028280022131994542 1103 22-23 -0.16698883218133886 1103 24-25 -0.26377421974444104 1103 26-27 0.11637854448394336 1103 28-29 0.1434820839040114 1103 30-31 0.23526982485299897 1103 32-33 0.3508958477232085 1103 34-35 0.4227708000273793 1103 36-37 0.3301589226144088 1103 38-39 0.5186698583321458 1103 40-41 0.5978005511235693 1103 42-43 0.8601593570031625 1103 44-45 0.5172717897371228 1103 46-47 0.850061336323769 1103 48-49 1.342546013092992 1103 50-51 1.090899026646241 1103 52-53 1.2125003246882926 1103 54-55 1.219883688740424 1103 56-57 0.9166937781786793 1103 58-59 0.7209583578260776 1103 60-61 0.9551933358420825 1103 62-63 1.4602896970035495 1103 64-65 1.6499926500256699 1103 66-67 1.4219367813527022 1103 68-69 1.276494662462209 1103 70-71 1.342441683380347 1103 72-73 1.2284105895358621 1103 74-75 1.307269362179401 1103 76-77 1.3015356015307482 1103 78-79 1.0735271814644491 1103 80-81 1.213194338556157 1103 82-83 0.806362380680639 1103 84-85 0.9011683554973047 1103 86-87 1.076162113310815 1103 88-89 1.2151144105271001 1103 90-91 1.3113755788912087 1103 92-93 1.5930469694045755 1103 94-95 1.6820242364125058 1103 96-97 1.4275665784570855 1103 98-99 1.247045320991102 1103 100-101 1.4857972552404526 1104 1 0.06177261202558881 1104 2 0.12693835243532448 1104 3 0.1589131574616971 1104 4 0.00323081121405977 1104 5 0.10499733233706365 1104 6 0.18260478207331232 1104 7 0.1770443806245936 1104 8 0.09767797309974924 1104 9 0.24264311961977825 1104 10-11 0.24557587593672991 1104 12-13 0.17299819081480905 1104 14-15 0.3609903645491457 1104 16-17 0.19298474468387639 1104 18-19 0.253476862612942 1104 20-21 0.23865464484796206 1104 22-23 0.3925087323547345 1104 24-25 0.13565676054841447 1104 26-27 0.21561438974632807 1104 28-29 0.19049696481206269 1104 30-31 -0.043382253856428576 1104 32-33 -0.0880313564833628 1104 34-35 0.18702608431379986 1104 36-37 0.25912455799491596 1104 38-39 0.13777298198220933 1104 40-41 0.02113184869704554 1104 42-43 -0.17577676978991974 1104 44-45 -0.13242359107410095 1104 46-47 0.005614491999814675 1104 48-49 0.3355189176257838 1104 50-51 0.258804527666598 1104 52-53 0.09238428637628004 1104 54-55 -0.0956652961105462 1104 56-57 -0.25445156017950055 1104 58-59 -0.07751631185946906 1104 60-61 -0.0022307327977806324 1104 62-63 0.08714981050957604 1104 64-65 0.10933455341148601 1104 66-67 0.06541316405569475 1104 68-69 -0.07783554771758361 1104 70-71 0.3727434723663876 1104 72-73 0.11332560897488975 1104 74-75 0.042373558327344796 1104 76-77 0.04348069559774714 1104 78-79 -0.08800663945819664 1104 80-81 0.04813766002249764 1104 82-83 -0.277340865115022 1104 84-85 0.07251106325652756 1104 86-87 0.3332115524837995 1104 88-89 0.18404921159364207 1104 90-91 0.2320516994280517 1104 92-93 0.14184189887294352 1104 94-95 0.38370224871643543 1104 96-97 0.2107656012923549 1104 98-99 0.017914342187832943 1104 100-101 0.4659597684604755 1105 1 -0.3157366972669067 1105 2 -0.32617921062987776 1105 3 -0.09966119088782577 1105 4 -0.2190302969417175 1105 5 -0.21195257946963864 1105 6 -0.30580409885173765 1105 7 -0.10805795159816256 1105 8 -0.254255279973016 1105 9 -0.29127630250413716 1105 10-11 -0.36041006913144713 1105 12-13 -0.33967784580640625 1105 14-15 -0.408860126813515 1105 16-17 -0.2700860619348191 1105 18-19 -0.3592985664544557 1105 20-21 -0.1622538861036773 1105 22-23 -0.3158024359964813 1105 24-25 -0.22391291215646447 1105 26-27 -0.2879623384223251 1105 28-29 0.31135677946614493 1105 30-31 -0.42746406363888667 1105 32-33 -0.2109980110176224 1105 34-35 0.08762663867166509 1105 36-37 -0.42351256158399764 1105 38-39 -0.29781791310976047 1105 40-41 -0.37104834560818745 1105 42-43 -0.19204353351587145 1105 44-45 -0.013649617896064115 1105 46-47 0.408013909644076 1105 48-49 0.29613291096229233 1105 50-51 0.38214215400699914 1105 52-53 -0.18597055865549805 1105 54-55 -0.14911323406726495 1105 56-57 0.31430421438163236 1105 58-59 0.3269829461969138 1105 60-61 0.13376893122775257 1105 62-63 -0.12605398600188877 1105 64-65 -0.611218684542429 1105 66-67 -0.25761228601009734 1105 68-69 -0.2058698171131077 1105 70-71 -0.12419776009994266 1105 72-73 -0.21809220327138235 1105 74-75 -0.26788486203270523 1105 76-77 -0.28856706877215643 1105 78-79 -0.11844732596602014 1105 80-81 -0.20597102771723286 1105 82-83 -0.4524535565504735 1105 84-85 -0.2811370594861451 1105 86-87 -0.10095145112792636 1105 88-89 0.059752658131703384 1105 90-91 0.24395079509678297 1105 92-93 0.09204068376529051 1105 94-95 0.314281524691463 1105 96-97 0.4599536629996628 1105 98-99 0.13806021110179145 1105 100-101 0.2006939286643039 1106 1 -0.003399470793553405 1106 2 0.10852343402041242 1106 3 0.06006494684878305 1106 4 0.12936546087812673 1106 5 0.16615186407982918 1106 6 0.1489012307227 1106 7 0.14161314519335377 1106 8 0.06722411323412558 1106 9 -0.02124005602809831 1106 10-11 0.19949058867496916 1106 12-13 0.2353456472799067 1106 14-15 0.22594991480280413 1106 16-17 0.267892190179559 1106 18-19 0.10306550926040359 1106 20-21 0.15072100985550918 1106 22-23 0.3959709564051863 1106 24-25 0.1710125811983545 1106 26-27 0.33809485046208465 1106 28-29 0.42469414018570006 1106 30-31 0.34523697005690934 1106 32-33 0.35056729976235346 1106 34-35 0.4487153754148494 1106 36-37 0.8487790211787924 1106 38-39 0.8395897926225544 1106 40-41 1.1028609039555164 1106 42-43 1.3051775699879542 1106 44-45 1.52589260253621 1106 46-47 1.338776428102932 1106 48-49 0.9570806891875634 1106 50-51 0.8850351597795836 1106 52-53 1.058632743801212 1106 54-55 0.9137341279947577 1106 56-57 0.8097264187043614 1106 58-59 0.8700155680253516 1106 60-61 1.0641891218573676 1106 62-63 1.1707710911896818 1106 64-65 0.7900969282915078 1106 66-67 0.9428710003370604 1106 68-69 0.9264768065947706 1106 70-71 0.5505303913297794 1106 72-73 0.5858814844719404 1106 74-75 0.885867319468165 1106 76-77 1.07121276744747 1106 78-79 0.9638992977418575 1106 80-81 1.011883717886203 1106 82-83 1.3091050419191212 1106 84-85 1.3976721766529359 1106 86-87 1.3775691556649328 1106 88-89 1.496965706863076 1106 90-91 1.6822847996611507 1106 92-93 1.5724877236364136 1106 94-95 1.807965240626487 1106 96-97 2.0822519580728276 1106 98-99 1.5878924033423623 1106 100-101 1.8206697643469418 1107 1 0.004292836898752483 1107 2 -0.14275861726164152 1107 3 -0.021986335202498708 1107 4 -0.042429410916746235 1107 5 -0.08513018720221766 1107 6 -0.07673979491832483 1107 7 -0.14813044455023316 1107 8 -0.06610922009920728 1107 9 0.03517020038215435 1107 10-11 7.726399570273657E-4 1107 12-13 0.059704621638886124 1107 14-15 -0.10097316212026897 1107 16-17 -0.1462103739230045 1107 18-19 -0.07513961894471777 1107 20-21 -0.2787661696316732 1107 22-23 -0.35018288974865897 1107 24-25 -0.18924382905805714 1107 26-27 -0.45421284184561017 1107 28-29 -0.6624853469937833 1107 30-31 -0.5945066196866762 1107 32-33 -0.21994552075047125 1107 34-35 -0.2051307784312968 1107 36-37 0.038522610922377964 1107 38-39 0.20112825416101288 1107 40-41 0.10157885267346245 1107 42-43 0.4179980828084666 1107 44-45 0.6848669615105649 1107 46-47 0.6554430947696019 1107 48-49 0.7340037661106393 1107 50-51 0.7363172110616318 1107 52-53 0.4624788976473653 1107 54-55 0.44963156389219705 1107 56-57 0.43664949562743516 1107 58-59 0.3815540295638087 1107 60-61 0.47572758339582677 1107 62-63 -0.012562242143651758 1107 64-65 -0.2688774306828492 1107 66-67 0.07876843623450114 1107 68-69 0.09955372967169396 1107 70-71 0.2505303913297787 1107 72-73 0.30383020242065584 1107 74-75 0.23714937075022036 1107 76-77 -0.1698128735781701 1107 78-79 -0.43994685610429585 1107 80-81 -0.058629102626618135 1107 82-83 -0.28961290679882623 1107 84-85 -0.1843791053983459 1107 86-87 0.055774283870061225 1107 88-89 0.05594006583743649 1107 90-91 0.370746338122693 1107 92-93 0.39941080055949385 1107 94-95 0.078478061139311 1107 96-97 -0.12159419577332287 1107 98-99 -0.5505691351191757 1107 100-101 -0.0716379279607473 1108 1 -0.007235837988698535 1108 2 0.01347547943046834 1108 3 -0.07827915180065759 1108 4 0.020988087615258166 1108 5 0.07846347104792528 1108 6 -0.02091673756854817 1108 7 0.17600343683555764 1108 8 0.12238167831535662 1108 9 -0.0051899483749409114 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-1.9107972779831996 2316 84-85 -2.12215165031375 2316 86-87 -2.1140582645339165 2316 88-89 -2.4832540733567043 2316 90-91 -2.5512404052926207 2316 92-93 -2.6504234921319423 2316 94-95 -2.602622584247051 2316 96-97 -2.986167717489703 2316 98-99 -3.048266675673858 2316 100-101 -3.4779784128726625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 16.0 5 61.0 6 172.0 7 350.0 8 539.0 9 921.0 10 1480.0 11 2401.0 12 3329.0 13 4524.0 14 5038.0 15 6452.0 16 7949.0 17 10308.0 18 9304.0 19 5901.0 20 5172.0 21 5269.0 22 5532.0 23 5657.0 24 5968.0 25 6515.0 26 6422.0 27 6764.0 28 7926.0 29 9097.0 30 10411.0 31 12225.0 32 14347.0 33 17791.0 34 23501.0 35 35882.0 36 42613.0 37 62305.0 38 48297.0 39 4393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.681447830364476 21.168341548857157 15.846512491070545 17.303698129707826 2 15.201033343042031 24.97701571542543 38.51862170273659 21.303329238795953 3 20.58682740891765 30.90303544518596 32.52447224790102 15.985664897995367 4 11.708435169121278 20.482480023300873 37.10613294160852 30.70295186596933 5 10.363063051654489 42.685425557511365 32.57360669646815 14.377904694366 6 24.05637798067547 38.51203667354718 18.8805450378006 18.55104030797675 7 22.12088593969633 39.88096293388377 20.98420859346309 17.013942532956804 8 24.61255967682703 32.41556599592235 21.00497676244507 21.966897564805553 9 25.94450846556156 19.151037775273217 22.795344890903795 32.10910886826143 10-11 22.779388858637155 29.07493003406486 26.212215229146352 21.933465878151633 12-13 27.698279027948384 27.822254865956666 26.871984499854367 17.607481606240583 14-15 23.904289133435487 28.384261780237313 29.755340838578142 17.956108247749057 16-17 17.521002763221546 32.072745416370125 30.85956198759463 19.546689832813698 18-19 16.73606309503005 30.244305820435475 35.38673312514405 17.632897959390426 20-21 19.1750984588499 28.117188192536126 34.22353641394507 18.484176934668913 22-23 18.417946737244673 28.92993275672116 33.94126660503755 18.710853900996618 24-25 18.71629921359555 29.69252979092532 34.67119176364811 16.919979231831018 26-27 18.855898934742196 28.812994828206286 35.90812847931029 16.42297775774123 28-29 16.881228867754885 29.91236845771018 36.5338685780136 16.672534096521332 30-31 17.387516304279004 30.877708409842086 35.29651626628845 16.43825901959046 32-33 16.911621310167536 31.02029961882812 34.845188496460544 17.222890574543797 34-35 17.652817495396413 29.407980222947767 34.94152750372972 17.997674777926097 36-37 18.01585230856526 29.872153081928925 35.34826696118489 16.763727648320916 38-39 18.107498828505932 29.05621905039324 35.748932990539394 17.08734913056143 40-41 16.570099151092386 29.942515415974864 36.357384660392626 17.130000772540125 42-43 16.352959501952146 31.040989409138053 34.62893991335325 17.977111175556548 44-45 16.400202112045548 31.07852610170693 33.62290907110773 18.89836271513979 46-47 17.531909181511608 30.523653761161405 32.05823243586188 19.886204621465104 48-49 17.650157660795017 30.303037977889495 32.123798548760874 19.92300581255461 50-51 17.392058657785263 30.949959793076808 32.62408742947959 19.03389411965834 52-53 16.736611495941343 31.480238580672935 32.58994263426494 19.19320728912077 54-55 15.757468309547026 30.709030354451862 32.38605999974673 21.147441336254385 56-57 17.420314815049323 31.632074157559487 30.094596477009382 20.853014550381804 58-59 17.254086527502697 30.590197399413427 31.009234260474024 21.146481812609856 60-61 17.26109519720996 30.708046186615608 31.074276972796095 20.95658164337834 62-63 18.044246330745757 30.820595945141644 30.67192624767308 20.463231476439525 64-65 17.475654387275696 31.238618663492346 31.114262919953905 20.171464029278052 66-67 16.727810583448466 30.892008910084883 30.9087248169165 21.471455689550154 68-69 16.522787453445567 31.439581494478052 30.761194275561728 21.276436776514657 70-71 17.225536592377217 31.022631287682405 29.76031426223218 21.9915178577082 72-73 17.053579343269973 29.944407157420187 30.117010903288715 22.885002596021124 74-75 16.832600959894638 30.88100092443679 29.924525434675243 22.36187268099333 76-77 17.357912269271427 29.529581171078217 30.733894949259362 22.378611610390994 78-79 16.607424710356238 29.417874940779154 31.338530411011657 22.636169937852948 80-81 16.715231318438484 30.287626841119508 30.312067461176774 22.685074379265235 82-83 16.63796269327694 30.078007268859142 30.00215279800423 23.28187723985969 84-85 16.828675269416337 29.065305760634192 29.82423037471349 24.281788595235984 86-87 17.05685109750468 29.35856723262432 29.728249014604653 23.85633265526635 88-89 17.366479135220843 28.712639822734502 29.589438269628545 24.331442772416107 90-91 16.653141981258468 28.659633830254407 30.078138393724547 24.60908579476258 92-93 16.81423885927033 29.49991768713513 29.279319209289955 24.406524244304585 94-95 16.742310078893716 29.295275241556602 28.95931211772006 25.00310256182963 96-97 16.614028644876974 28.824319019337192 28.63803867438297 25.923613661402868 98-99 17.114870768802156 28.917395874225942 28.129598439854625 25.838134917117277 100-101 17.413669306175894 28.584074046401835 28.900156648308716 25.102099999113552 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5872.0 1 3736.0 2 1687.5 3 1684.0 4 1371.5 5 1314.0 6 2043.0 7 3297.5 8 4217.0 9 4205.5 10 3297.0 11 2419.5 12 2094.5 13 1912.5 14 1837.5 15 1763.5 16 1619.5 17 1589.5 18 1632.0 19 1700.5 20 1834.0 21 2012.0 22 2198.5 23 2401.0 24 2689.0 25 3096.5 26 3780.5 27 4563.5 28 5041.5 29 5481.0 30 6148.0 31 6851.0 32 7622.0 33 8393.0 34 9130.5 35 9821.0 36 10599.5 37 11237.5 38 10944.5 39 10121.5 40 9709.5 41 9535.5 42 9377.5 43 9615.5 44 10149.0 45 10638.5 46 12047.5 47 16054.5 48 23599.5 49 24013.5 50 17622.5 51 12765.5 52 8527.5 53 6630.0 54 5938.0 55 5486.0 56 5042.0 57 4479.0 58 4038.5 59 3716.0 60 3238.5 61 2831.0 62 2437.0 63 1742.0 64 1155.5 65 876.5 66 644.5 67 497.0 68 412.5 69 306.5 70 238.0 71 220.5 72 193.0 73 197.0 74 185.0 75 122.0 76 75.5 77 47.5 78 34.0 79 22.5 80 14.5 81 11.5 82 8.0 83 4.5 84 2.5 85 2.0 86 2.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3733205009687591 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0015196221206326694 18-19 5.065407068775564E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.532703534387782E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00861119201691846 36-37 0.016715843326959363 38-39 0.010130814137551129 40-41 0.00848455684019907 42-43 0.003419149771423506 44-45 0.001139716590474502 46-47 8.864462370357237E-4 48-49 0.0 50-51 6.331758835969455E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.532703534387782E-4 60-61 5.065407068775564E-4 62-63 0.0 64-65 0.0 66-67 3.7990553015816736E-4 68-69 3.7990553015816736E-4 70-71 0.0013929869439132801 72-73 0.0 74-75 0.0 76-77 0.09573619359985817 78-79 0.03191206453328606 80-81 1.266351767193891E-4 82-83 0.0 84-85 0.0 86-87 0.14613699393417504 88-89 0.3295047298238505 90-91 0.28125672749376324 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.7990553015816736E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 394835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.188046921395845 #Duplication Level Percentage of deduplicated Percentage of total 1 83.35228628675871 40.16583882591948 2 9.016851961934082 8.690089708499194 3 2.7292134128506804 3.945463919908544 4 1.294666786384561 2.4954985539948797 5 0.7783630836850625 1.8753898399249076 6 0.471420989027216 1.3630114043384591 7 0.3662973887890282 1.2355809030705327 8 0.2699977456649395 1.0408531229418565 9 0.22168702968815096 0.9614398489629743 >10 1.317203632121596 11.993813969907071 >50 0.09784443079724578 3.2829615387217106 >100 0.0631254392240295 5.768440489163093 >500 0.012625087844805899 4.445471122273386 >1k 0.006838589249269862 7.747444483665477 >5k 0.0015781359806007374 4.988702268708458 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6976 1.766813985588917 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6872 1.740473868831284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5832 1.4770727012549545 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4537 1.1490875935517368 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4230 1.0713335950460319 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3229 0.8178099712538149 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3051 0.7727278483417124 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2854 0.7228335887142731 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2618 0.6630617853027214 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1675 0.42422784200995356 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1665 0.4216951384755657 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1633 0.4135904871655248 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1459 0.36952144566717743 RNA PCR Primer, Index 33 (95% over 22bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1347 0.3411551660820343 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1151 0.2915141768080337 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1114 0.28214317373079895 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 966 0.24465916142185976 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 958 0.24263299859434953 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 923 0.2337685362239923 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 902 0.22844985880177798 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 895 0.22667696632770654 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 858 0.21730596325047172 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 828 0.20970785264730835 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 792 0.20059011992351236 RNA PCR Primer, Index 33 (95% over 24bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 789 0.19983030886319603 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 777 0.19679106462193066 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 777 0.19679106462193066 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 708 0.17931541023465497 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 702 0.17779578811402233 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 696 0.17627616599338963 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 680 0.17222384033836918 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 679 0.1719705699849304 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 661 0.1674117036230324 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 632 0.16006686337330783 RNA PCR Primer, Index 33 (96% over 29bp) TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 626 0.1585472412526752 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 574 0.1453771828738587 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 552 0.13980523509820558 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 531 0.13448655767599124 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 517 0.13094077272784835 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 514 0.130180961667532 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 491 0.1243557435384401 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 489 0.12384920283156256 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 444 0.11245203692681752 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 437 0.11067914445274608 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 412 0.10434738561677663 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 411 0.10409411526333785 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5327035343877824E-4 0.0 14-15 0.0 0.0 0.0 8.864462370357239E-4 0.0 16-17 0.0 0.0 0.0 0.001266351767193891 0.0 18-19 0.0 0.0 0.0 0.002152798004229615 0.0 20-21 0.0 0.0 0.0 0.004685501538617397 0.0 22-23 0.0 0.0 0.0 0.01253688249521952 0.0 24-25 0.0 0.0 0.0 0.025327035343877823 0.0 26-27 0.0 0.0 0.0 0.0464751098560158 0.0 28-29 0.0 0.0 0.0 0.09852216748768472 0.0 30-31 0.0 0.0 0.0 0.21198728582825738 0.0 32-33 0.0 0.0 0.0 0.41017133739410133 0.0 34-35 0.0 0.0 0.0 0.7330910380285436 0.0 36-37 0.0 0.0 0.0 1.1822660098522166 0.0 38-39 0.0 0.0 0.0 1.7090683450048756 0.0 40-41 0.0 0.0 0.0 2.222067445895121 0.0 42-43 0.0 0.0 0.0 2.7029012118986415 0.0 44-45 0.0 0.0 0.0 3.237428292831183 0.0 46-47 0.0 0.0 0.0 3.8662985804196692 0.0 48-49 0.0 0.0 0.0 4.5071992097964975 0.0 50-51 0.0 0.0 0.0 5.1085896640368755 0.0 52-53 0.0 0.0 0.0 5.646915800271 0.0 54-55 0.0 0.0 0.0 6.216520825154811 0.0 56-57 0.0 0.0 0.0 6.862866767130574 0.0 58-59 0.0 0.0 0.0 7.560373320500968 0.0 60-61 0.0 0.0 0.0 8.423012144313446 0.0 62-63 0.0 0.0 0.0 9.18928159864247 0.0 64-65 0.0 0.0 0.0 9.89033393696101 0.0 66-67 0.0 0.0 0.0 10.685602846758773 0.0 68-69 0.0 0.0 0.0 11.55090100928236 0.0 70-71 0.0 0.0 0.0 12.43798042220168 0.0 72-73 0.0 0.0 0.0 13.362923752960096 0.0 74-75 0.0 0.0 0.0 14.301163777274052 0.0 76-77 0.0 0.0 0.0 15.165195588030443 0.0 78-79 0.0 0.0 0.0 16.16498030823002 0.0 80-81 0.0 0.0 0.0 17.223270480073957 0.0 82-83 0.0 0.0 0.0 18.216343535907406 0.0 84-85 0.0 0.0 0.0 19.375055402889814 0.0 86-87 0.0 0.0 0.0 20.514645358187597 0.0 88-89 0.0 0.0 0.0 21.523421175934253 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTCC 30 1.3077533E-4 63.25895 9 TGCGCCG 25 0.005042239 56.933056 5 GCCGGTC 55 4.991864E-5 43.131104 8 CGCCGGT 50 0.0016238786 37.95537 7 GACATAT 75 3.0074318E-4 31.786474 1 TTCGACC 30 0.0039729984 31.629475 16-17 TCGTATC 40 4.523403E-4 29.652634 94-95 TTAGGGA 145 2.419074E-8 29.448132 4 TAGGGAG 175 5.002221E-9 27.110977 5 CGCGGCA 45 8.9999806E-4 26.361235 36-37 ATGGGAT 690 0.0 26.128695 5 GTCGGGA 140 1.7918677E-5 23.722105 2 GTACATG 5110 0.0 23.046751 1 CGGGAGT 145 2.3505805E-5 22.904102 4 ATGGGTA 125 2.2702533E-4 22.773224 5 TATAATG 125 2.2702533E-4 22.773224 2 CATGGGG 1105 0.0 22.756046 4 ATGGGGG 480 0.0 22.733685 5 GTACTGA 105 0.002146048 22.704624 1 TACATGG 5005 0.0 22.655678 2 >>END_MODULE