##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392213 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28210181712488 33.0 31.0 34.0 30.0 34.0 2 31.23707016340611 33.0 31.0 34.0 28.0 34.0 3 31.18142947837017 33.0 31.0 34.0 28.0 34.0 4 34.76734835408311 37.0 35.0 37.0 32.0 37.0 5 34.818093739881135 37.0 35.0 37.0 32.0 37.0 6 34.927789236970725 37.0 35.0 37.0 32.0 37.0 7 34.89075833794392 37.0 35.0 37.0 32.0 37.0 8 34.85906637464847 37.0 35.0 37.0 32.0 37.0 9 36.32904569710846 39.0 37.0 39.0 32.0 39.0 10-11 36.214847544574 39.0 37.0 39.0 32.0 39.0 12-13 36.091037268015086 39.0 37.0 39.0 31.5 39.0 14-15 37.027645692519116 40.0 37.0 41.0 31.0 41.0 16-17 36.92974608184838 39.5 36.5 41.0 31.0 41.0 18-19 36.98108043333597 40.0 36.5 41.0 31.5 41.0 20-21 36.92737492128002 40.0 36.0 41.0 31.0 41.0 22-23 36.82004421067124 40.0 36.0 41.0 31.0 41.0 24-25 36.67406485761563 40.0 36.0 41.0 31.0 41.0 26-27 36.41786988192641 39.0 35.0 41.0 30.0 41.0 28-29 36.14645103553427 39.0 35.0 41.0 30.0 41.0 30-31 35.73558882545963 38.5 35.0 40.5 29.5 41.0 32-33 35.30452840675857 38.0 35.0 40.0 28.0 41.0 34-35 34.847965263772494 38.0 35.0 40.0 25.5 41.0 36-37 34.46604523562452 38.0 35.0 40.0 24.0 41.0 38-39 34.12559884552526 37.5 34.5 40.0 23.5 41.0 40-41 33.76587084058917 37.0 34.0 40.0 20.0 41.0 42-43 33.44378692190213 38.0 33.0 40.0 15.5 41.0 44-45 32.95153271309212 37.0 33.0 40.0 5.0 41.0 46-47 32.21720213251473 37.0 33.0 40.0 2.0 41.0 48-49 31.56487928752999 37.0 31.5 40.0 2.0 41.0 50-51 30.29548357652602 36.0 29.0 39.0 2.0 40.0 52-53 30.276589506212186 35.5 29.5 39.0 2.0 40.0 54-55 30.635663529765715 36.0 31.0 40.0 2.0 41.0 56-57 30.44006317995579 36.0 30.0 40.0 2.0 41.0 58-59 30.178275579850744 35.5 29.5 40.0 2.0 41.0 60-61 29.786199080601612 35.0 28.0 39.5 2.0 41.0 62-63 29.322040574891705 35.0 27.5 39.0 2.0 41.0 64-65 28.846495399183606 35.0 26.0 39.0 2.0 41.0 66-67 28.36899847786789 35.0 25.5 38.5 2.0 40.0 68-69 27.86459525818879 35.0 23.5 37.5 2.0 40.0 70-71 27.33967640032329 35.0 22.0 37.0 2.0 39.0 72-73 26.815247072381588 34.0 19.5 36.5 2.0 39.0 74-75 26.286578976219552 34.0 15.5 36.0 2.0 39.0 76-77 25.742477939282992 34.0 6.0 35.0 2.0 37.5 78-79 25.22497979414247 33.5 2.0 35.0 2.0 37.0 80-81 24.676775119641622 33.0 2.0 35.0 2.0 36.5 82-83 24.20934925665391 33.0 2.0 35.0 2.0 36.0 84-85 23.78800932146563 33.0 2.0 35.0 2.0 36.0 86-87 23.265828771611346 32.0 2.0 35.0 2.0 35.0 88-89 22.690151779772727 32.0 2.0 35.0 2.0 35.0 90-91 22.131283256801787 31.0 2.0 35.0 2.0 35.0 92-93 21.609325800011728 31.0 2.0 35.0 2.0 35.0 94-95 21.03938803660256 30.5 2.0 35.0 2.0 35.0 96-97 20.397361892645065 29.5 2.0 34.0 2.0 35.0 98-99 19.684927577617266 29.0 2.0 34.0 2.0 35.0 100-101 17.782341482816733 23.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.272962913418727 1101 2 0.6720611015715257 1101 3 0.7043900357600137 1101 4 0.5319410375909541 1101 5 0.5918999163991785 1101 6 0.6227729346210324 1101 7 0.8173494063453504 1101 8 0.8845374726735713 1101 9 0.8738347469434089 1101 10-11 0.9557110084101197 1101 12-13 0.9676492715165708 1101 14-15 1.1327582834715528 1101 16-17 1.113324219080063 1101 18-19 0.8364387054160929 1101 20-21 0.9503382630699946 1101 22-23 0.45533325159590987 1101 24-25 0.7529120966627971 1101 26-27 0.8397016906714398 1101 28-29 0.6762347724484741 1101 30-31 0.4915979915093871 1101 32-33 0.7672870277360389 1101 34-35 0.5562907927887011 1101 36-37 0.5237487000160783 1101 38-39 0.3130746692321438 1101 40-41 0.6262877155688713 1101 42-43 0.8141714637560256 1101 44-45 0.7427185770447409 1101 46-47 1.1180383306738904 1101 48-49 1.3733673409562321 1101 50-51 1.2979123948327675 1101 52-53 1.5330990990953524 1101 54-55 1.3257560473464984 1101 56-57 1.4687267877137806 1101 58-59 1.3644386917128024 1101 60-61 1.2290902402282065 1101 62-63 1.4639834642462972 1101 64-65 1.4383122997094553 1101 66-67 1.013325267507934 1101 68-69 1.1353397693922318 1101 70-71 0.9338613680775474 1101 72-73 0.9663165414457708 1101 74-75 0.8531128865163815 1101 76-77 0.9343341325274253 1101 78-79 1.1625014207118056 1101 80-81 1.1077042828949217 1101 82-83 0.6239154827171447 1101 84-85 0.6909239685894413 1101 86-87 1.1617591025692207 1101 88-89 1.0459919944289027 1101 90-91 1.139005852739313 1101 92-93 1.138930798478146 1101 94-95 0.6580846710842181 1101 96-97 0.43684125007838404 1101 98-99 0.688164389652421 1101 100-101 0.7465325349431637 1102 1 0.14538460936983455 1102 2 -0.1256923432407433 1102 3 0.0535256496015073 1102 4 0.22224746319150057 1102 5 0.17109523088861067 1102 6 0.12328222924796961 1102 7 0.07638231243707594 1102 8 -0.008609685179472137 1102 9 0.39182982376201636 1102 10-11 0.2516819220280908 1102 12-13 0.14233732855757353 1102 14-15 0.335372662556523 1102 16-17 0.40667085066198894 1102 18-19 0.6488838855083259 1102 20-21 0.3783084410401756 1102 22-23 0.26330088309295974 1102 24-25 0.18507688176875803 1102 26-27 0.38108895264692677 1102 28-29 0.5352450432234477 1102 30-31 0.49377664074685157 1102 32-33 0.5570756704658635 1102 34-35 0.6262127009458993 1102 36-37 0.7831494300050466 1102 38-39 0.8335879250395166 1102 40-41 0.8015840967476038 1102 42-43 1.1704230553017325 1102 44-45 1.1062559111703152 1102 46-47 0.31748376541343504 1102 48-49 0.6822333115869093 1102 50-51 0.7957974074236631 1102 52-53 0.5467580980484641 1102 54-55 0.6166269411585716 1102 56-57 0.2547523090334245 1102 58-59 0.26649850553732435 1102 60-61 0.07184552415995782 1102 62-63 -0.10788366644436209 1102 64-65 -0.43010577459096666 1102 66-67 -0.3595491347782307 1102 68-69 -0.6868527439767504 1102 70-71 -0.7452971640221584 1102 72-73 -0.589861485320494 1102 74-75 -0.8699723071570453 1102 76-77 -0.6055110984942758 1102 78-79 -0.5554059442543853 1102 80-81 -0.6661335350605384 1102 82-83 -0.6399274281845848 1102 84-85 -0.8159585824107545 1102 86-87 -0.7119146093397859 1102 88-89 -0.8579918213196187 1102 90-91 -1.087128035159278 1102 92-93 -1.4229626457682407 1102 94-95 -1.4510387762745225 1102 96-97 -1.6868395353671062 1102 98-99 -1.5190194940020518 1102 100-101 -1.2454177805365667 1103 1 -0.017537822958299643 1103 2 -0.024074282708191674 1103 3 0.07567405910589287 1103 4 0.011486120228823893 1103 5 -0.14533621516685002 1103 6 -0.2057739480495684 1103 7 0.1441644393459498 1103 8 -0.0056878041496446485 1103 9 0.20933630151712634 1103 10-11 0.1600079047173253 1103 12-13 0.1652437732347849 1103 14-15 -0.2395212125192856 1103 16-17 0.012733753798876535 1103 18-19 0.04678343895670167 1103 20-21 0.2572915713119599 1103 22-23 0.10365867569453258 1103 24-25 0.1161232459564232 1103 26-27 -0.09701761331591285 1103 28-29 -0.12447296769224891 1103 30-31 -0.3332397328128778 1103 32-33 -0.4271778716257657 1103 34-35 -0.37960349619837785 1103 36-37 -0.3616628529212491 1103 38-39 -0.517285882777891 1103 40-41 -0.42371773907575516 1103 42-43 -0.5520533766956248 1103 44-45 -0.5405479368918762 1103 46-47 -0.579078889639213 1103 48-49 -0.4128174078573785 1103 50-51 -0.11644968447776449 1103 52-53 -0.36901730302105307 1103 54-55 -0.30108489521608917 1103 56-57 -0.30429863093019094 1103 58-59 -0.5425868905705116 1103 60-61 -1.0234257146073382 1103 62-63 -0.8499449708161606 1103 64-65 -0.8633090934067802 1103 66-67 -0.9876088403849366 1103 68-69 -0.9870829110201385 1103 70-71 -1.0841457775069152 1103 72-73 -1.0013987806509945 1103 74-75 -0.7652256287293504 1103 76-77 -0.8762192411702756 1103 78-79 -0.8330053157743116 1103 80-81 -0.6492844805371405 1103 82-83 -0.39620533766140653 1103 84-85 -0.6191657603147185 1103 86-87 -0.371398318474796 1103 88-89 -0.6067789650430875 1103 90-91 -0.8476302679174275 1103 92-93 -0.7995476284023404 1103 94-95 -1.0409348565435543 1103 96-97 -1.0015826303558057 1103 98-99 -0.8215857876235297 1103 100-101 -0.5744119367745029 1104 1 0.13382600865682193 1104 2 0.09534152485194625 1104 3 0.23989764158011795 1104 4 0.1679777439401633 1104 5 0.3902630116372734 1104 6 -0.03682527966468285 1104 7 -0.0688939798980357 1104 8 -0.16349493473383347 1104 9 -0.06912160226293906 1104 10-11 -0.0014235815369687543 1104 12-13 -0.11047572848342924 1104 14-15 0.14013675569377426 1104 16-17 0.4054662495033412 1104 18-19 0.17880395012507933 1104 20-21 0.16429328952502686 1104 22-23 0.07181803995569425 1104 24-25 0.19185885592206375 1104 26-27 0.3384822792957678 1104 28-29 0.40695153832148634 1104 30-31 0.5938838711390133 1104 32-33 0.33611143249794395 1104 34-35 0.48083196342361134 1104 36-37 0.6291554460478253 1104 38-39 0.5083665012427261 1104 40-41 0.8220135753572322 1104 42-43 0.634855987565544 1104 44-45 0.3873945029706718 1104 46-47 0.1391610158590737 1104 48-49 0.3042949203213112 1104 50-51 0.49305955091742604 1104 52-53 0.6075451969789469 1104 54-55 0.32203580925126474 1104 56-57 0.32299679432754047 1104 58-59 0.40596068245354644 1104 60-61 0.17365869260915545 1104 62-63 0.3710601838230154 1104 64-65 0.48566266346606923 1104 66-67 0.2455714645978837 1104 68-69 0.6770064360588961 1104 70-71 0.8049757860669651 1104 72-73 0.48979537742460977 1104 74-75 0.9003805783153211 1104 76-77 0.5700194830565266 1104 78-79 0.37798587838376463 1104 80-81 0.8391444283975673 1104 82-83 0.7020818186959836 1104 84-85 0.8227237371079603 1104 86-87 0.9817615827279482 1104 88-89 1.101030850249007 1104 90-91 1.137993121257832 1104 92-93 1.3191970021818484 1104 94-95 1.6059186657932116 1104 96-97 1.563494689231824 1104 98-99 1.5299095946788768 1104 100-101 1.7152411989643248 1105 1 0.10762571254852915 1105 2 0.3736833184221666 1105 3 0.32797663904204555 1105 4 0.5707775535848327 1105 5 0.5424679608758538 1105 6 0.8061183540070544 1105 7 0.46071684920517697 1105 8 0.6082637370089756 1105 9 0.5466250136423056 1105 10-11 0.7291310927439056 1105 12-13 0.6635037554421288 1105 14-15 0.6248620623773675 1105 16-17 0.8840182270837218 1105 18-19 0.813804214507492 1105 20-21 0.7454058106756136 1105 22-23 0.8126439705817532 1105 24-25 0.7503270242807716 1105 26-27 1.148292188348229 1105 28-29 0.9818709016886658 1105 30-31 1.039163323338677 1105 32-33 0.876363653310122 1105 34-35 0.6905466948110899 1105 36-37 1.0438921211747285 1105 38-39 0.91792789083663 1105 40-41 0.6612029789105307 1105 42-43 0.7952567912880539 1105 44-45 1.0793890038166936 1105 46-47 1.1462861216120288 1105 48-49 0.9924955336884871 1105 50-51 1.0140647328125176 1105 52-53 1.0281908188063582 1105 54-55 1.0728553946996549 1105 56-57 0.8788938438199274 1105 58-59 0.9482420382065051 1105 60-61 1.1243645936243851 1105 62-63 0.9322911482900196 1105 64-65 1.2889555462917919 1105 66-67 1.3643915259346002 1105 68-69 1.3409235257374092 1105 70-71 1.1889912259992848 1105 72-73 1.4550026532283304 1105 74-75 1.3372130127484887 1105 76-77 1.4219190706199782 1105 78-79 1.4797334460656586 1105 80-81 1.3987230028476532 1105 82-83 1.2691159769024125 1105 84-85 1.5914221825394321 1105 86-87 1.723982130528288 1105 88-89 1.8819305435654208 1105 90-91 1.9164076199887958 1105 92-93 1.8891658050583295 1105 94-95 1.899832582882894 1105 96-97 1.8150809836480661 1105 98-99 1.8504666484013796 1105 100-101 2.3669834789981685 1106 1 0.3707072743209814 1106 2 0.41614352986447756 1106 3 0.5194833245375108 1106 4 0.30094688554417104 1106 5 0.5881405179029358 1106 6 0.7393142880044863 1106 7 0.5163190526834143 1106 8 0.4721175339378405 1106 9 0.5835256408448259 1106 10-11 0.960375447285081 1106 12-13 0.7101304745240995 1106 14-15 1.1062336385258575 1106 16-17 0.9764542664206388 1106 18-19 0.7496940660398721 1106 20-21 0.7932093296252773 1106 22-23 0.7869261226624644 1106 24-25 0.6111688496563943 1106 26-27 0.8681756533559266 1106 28-29 0.6332105452137213 1106 30-31 0.316716342943522 1106 32-33 0.18332712798666506 1106 34-35 0.5984795981353983 1106 36-37 0.2716601452959466 1106 38-39 0.5955865043755537 1106 40-41 0.6057502608208907 1106 42-43 0.8668030427033955 1106 44-45 1.1235317210157802 1106 46-47 0.8126220120996734 1106 48-49 0.853108360170932 1106 50-51 1.1331950459048912 1106 52-53 0.926722045349873 1106 54-55 0.8884126889505133 1106 56-57 1.058047311244831 1106 58-59 1.1340014875913909 1106 60-61 1.2729498893510076 1106 62-63 1.2725326149007081 1106 64-65 1.2932969836415076 1106 66-67 1.031843786026453 1106 68-69 1.3909788671365924 1106 70-71 1.3480913875707294 1106 72-73 1.0422703147679648 1106 74-75 0.8406511766862472 1106 76-77 0.9227078445853465 1106 78-79 1.2116520751256168 1106 80-81 1.1874174547133585 1106 82-83 1.1052577272172854 1106 84-85 0.8585652941255013 1106 86-87 0.8493113164372232 1106 88-89 1.196924882203895 1106 90-91 1.0629128424357752 1106 92-93 1.0337372590740834 1106 94-95 1.430989154515018 1106 96-97 0.9806312807105186 1106 98-99 0.7898073860322583 1106 100-101 1.2050399145031747 1107 1 0.09757394595055047 1107 2 0.2570576964691078 1107 3 0.20624974554051434 1107 4 0.12195361027679752 1107 5 0.14010830866697432 1107 6 -0.006529281314847424 1107 7 0.29084199369932406 1107 8 0.21557891015065422 1107 9 0.09971090598788379 1107 10-11 0.2440642484960307 1107 12-13 0.05634564115353413 1107 14-15 0.19573958160136584 1107 16-17 0.23368149290268292 1107 18-19 0.38642822817789124 1107 20-21 0.41037414761083113 1107 22-23 0.3794191125629638 1107 24-25 -0.0653863998535229 1107 26-27 -0.1686211654237013 1107 28-29 0.1743685143940965 1107 30-31 0.041607159092805546 1107 32-33 -0.21161443383869027 1107 34-35 -0.2140808872364488 1107 36-37 -0.05942747474425403 1107 38-39 -0.5158058378661892 1107 40-41 -0.2688744196922741 1107 42-43 -0.6512929398271936 1107 44-45 -0.17252711419104827 1107 46-47 0.10231592509999388 1107 48-49 -0.0687197248932101 1107 50-51 0.045168668079138996 1107 52-53 -0.22297769906065668 1107 54-55 -0.09963292012167457 1107 56-57 -0.17317430715760906 1107 58-59 -0.5301340988995911 1107 60-61 -0.4682812826383689 1107 62-63 -0.46866753894926205 1107 64-65 -0.27425121854713197 1107 66-67 -0.5845517821017872 1107 68-69 -0.6394127058552925 1107 70-71 -0.9460039994115803 1107 72-73 -0.5595033024433782 1107 74-75 -0.3453521980873191 1107 76-77 -0.40325557882466256 1107 78-79 -0.3530114400980864 1107 80-81 -0.11766765906117627 1107 82-83 -0.09909908064395268 1107 84-85 -0.0863495920999604 1107 86-87 -0.46286764581990525 1107 88-89 -0.039194500411056765 1107 90-91 -0.01821820299959498 1107 92-93 0.03116585531716609 1107 94-95 0.2596418506116933 1107 96-97 0.2324303327961772 1107 98-99 0.4917882044148456 1107 100-101 0.9951246560600389 1108 1 -0.1681465090736758 1108 2 -0.16618329784308727 1108 3 -0.046172837143284795 1108 4 -0.15532677733643396 1108 5 -0.6213726266606017 1108 6 -0.2892078151256783 1108 7 -0.3596741217420103 1108 8 -0.2258841191309955 1108 9 -0.4097687653835109 1108 10-11 -0.0938992021043461 1108 12-13 -0.014620232029521674 1108 14-15 0.10187766881434612 1108 16-17 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82-83 1.378334035008038 1108 84-85 1.218750997746259 1108 86-87 1.1162086128697943 1108 88-89 1.036563873298654 1108 90-91 1.2226839457259153 1108 92-93 1.0177231546641217 1108 94-95 1.3305418927435682 1108 96-97 1.4278563913594837 1108 98-99 1.5399107028349022 1108 100-101 1.6109083088934035 1109 1 -0.09156910628570714 1109 2 0.11176188609989524 1109 3 -0.059247986498700556 1109 4 -0.047424041774121406 1109 5 -0.20941371250065544 1109 6 -0.07755547260393314 1109 7 -0.11158691914270236 1109 8 -0.252754992205098 1109 9 0.12971870972392452 1109 10-11 -0.08333296166833293 1109 12-13 -0.2634673130482881 1109 14-15 -0.3929669491502281 1109 16-17 -0.05165479934722583 1109 18-19 -0.2571117415826336 1109 20-21 -0.41524283526972283 1109 22-23 -0.30392600766334965 1109 24-25 -0.4845445718283514 1109 26-27 -0.3840713434464291 1109 28-29 -0.03531421496258247 1109 30-31 -0.140677140765753 1109 32-33 -0.13952742218185676 1109 34-35 -0.0768509696634112 1109 36-37 -0.4161801450851783 1109 38-39 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-0.9051831398674821 2316 98-99 -0.9568861376152 2316 100-101 -0.8450275173559199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5288.0 3 922.0 4 761.0 5 739.0 6 817.0 7 1009.0 8 1224.0 9 1475.0 10 1765.0 11 2632.0 12 3745.0 13 5017.0 14 5480.0 15 6780.0 16 8126.0 17 10013.0 18 8838.0 19 5403.0 20 5180.0 21 5236.0 22 5224.0 23 5660.0 24 6037.0 25 6477.0 26 6987.0 27 7902.0 28 8706.0 29 9674.0 30 10744.0 31 12339.0 32 15032.0 33 17792.0 34 22931.0 35 35399.0 36 38960.0 37 52612.0 38 43197.0 39 6090.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.813472191333645 20.05618738672413 15.403217354182482 16.727123067759738 2 15.322457068720693 24.532889292974698 38.90529068638969 21.239362951914924 3 20.772502586504196 30.88119962703248 31.64859306944604 16.69770471701728 4 11.757329655736031 19.18301154356931 38.685003575441826 30.374655225252834 5 10.816260295572041 41.59372771901489 32.6445341149833 14.945477870429762 6 23.9669548157685 37.840261173441235 19.812294361678212 18.38048964911205 7 22.681213310741565 39.5726476067806 21.856256053647776 15.88988302883006 8 24.31569692849512 31.465929246512676 21.427950745513375 22.79042307947883 9 27.058586209361945 18.95855250390677 23.40409776624756 30.578763520483726 10-11 22.863843708125383 28.630644969681025 26.53954879841807 21.96596252377552 12-13 27.922677666482286 27.817670135832735 27.156838072511402 17.102814125173577 14-15 24.187161357138205 27.874864798254094 30.642494020475326 17.29547982413238 16-17 17.32746806574009 31.48452121621207 31.57733255607032 19.61067816197752 18-19 16.652931262928846 29.933341766232374 36.26964322448505 17.14408374635373 20-21 19.213466785199717 27.842238639731054 35.04950798565341 17.894786589415816 22-23 18.433891668924204 28.056874581503877 34.743484238988756 18.76574951058316 24-25 18.445920909729765 28.618667348112183 35.714559509359226 17.220852232798826 26-27 18.880645697693755 27.42961664897223 36.77713964108008 16.91259801225393 28-29 16.884729386551548 29.031581585549553 37.41416657502577 16.669522452873135 30-31 17.043414896065045 30.18524200352842 35.96008795479533 16.81125514561121 32-33 16.704856055381 30.404628707180624 35.47551536516208 17.41499987227629 34-35 17.79781467603295 29.017653252207776 35.19929791254354 17.98523415921573 36-37 18.193651106506444 28.91072052505142 35.940885640309574 16.954742728132562 38-39 18.251046421309503 28.877133311067688 35.91435660605037 16.95746366157244 40-41 16.786713380635703 29.719396402308067 36.50980647983637 16.984083737219855 42-43 16.175315219866977 30.951302465202335 34.61379008544909 18.2595922294816 44-45 16.52948508278243 30.679151214543293 33.85761780084612 18.93374590182815 46-47 17.200741220403014 30.5544820031379 32.625471261072875 19.61930551538621 48-49 17.533725102176913 30.341007855430384 32.561059747095335 19.56420729529737 50-51 17.65186228081403 30.76052732625112 32.84794573147319 18.739664661461667 52-53 16.845429016841205 31.249792012778737 32.61239629437168 19.292382676008383 54-55 16.028499319128894 30.33344766507285 32.92498131443959 20.713071701358672 56-57 17.31865949608907 31.15375412493746 30.977304686850232 20.55028169212324 58-59 17.730833044441574 29.33340838122967 31.59328815961664 21.342470414712118 60-61 17.45485515669778 28.948455401204377 32.56899446663163 21.02769497546622 62-63 18.651424856708132 28.51428183869156 32.307245404998675 20.527047899601637 64-65 18.0807594625594 29.141405865967556 32.77537686383746 20.00245780763559 66-67 17.18680789723658 28.9325519053069 32.50991507218992 21.3707251252666 68-69 16.761533123884583 29.590909207295375 32.44612071724674 21.2014369515733 70-71 17.28153400834958 29.15414720201967 31.742107776427858 21.82221101320289 72-73 17.041439660822547 27.977531541864657 32.257829141405864 22.72319965590693 74-75 16.83563488528082 28.825722083122123 32.34870944122722 21.989933590369837 76-77 17.584850699372748 27.540913529653434 32.685901070336584 22.188334700637235 78-79 16.925444977141783 27.55845063123995 33.55174350687154 21.964360884746732 80-81 17.07314576303286 28.363833661562165 32.595106383250986 21.967914192153987 82-83 16.906709598816466 27.728606019032796 32.41181077774422 22.952873604406516 84-85 17.127642420007525 26.689625869928562 32.484713061150316 23.698018648913596 86-87 17.653532907770195 26.70835274303938 32.50336577100472 23.13474857818571 88-89 18.03330722407376 25.878499792661763 32.47467145848372 23.613521524780758 90-91 17.515575815530116 25.69436710408242 33.13913968501458 23.650917395372893 92-93 17.73874351313502 26.331871468546865 32.31950066180744 23.609884356510673 94-95 17.574779964794367 25.938486157785245 32.044423107697234 24.442310769723154 96-97 17.56997404978294 24.927059022341254 31.870886468120407 25.6320804597554 98-99 18.147986942328618 24.888561735902197 31.578442040581194 25.385009281187994 100-101 18.85269190700103 23.919487241182477 32.710132054849225 24.517688796967267 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6308.0 1 3981.0 2 1799.0 3 1822.0 4 1433.0 5 1352.0 6 2028.5 7 3222.0 8 4397.5 9 4568.0 10 3488.0 11 2466.0 12 2176.0 13 2062.0 14 1953.5 15 1831.0 16 1707.5 17 1686.5 18 1697.5 19 1745.0 20 1857.0 21 2016.0 22 2226.5 23 2469.5 24 2740.5 25 3197.5 26 3852.5 27 4546.5 28 5127.0 29 5569.0 30 6132.5 31 6759.0 32 7452.0 33 8258.5 34 9071.0 35 9680.0 36 10400.5 37 11063.5 38 10734.5 39 10033.0 40 9559.0 41 9176.0 42 8908.0 43 9072.5 44 9446.0 45 9879.5 46 11692.5 47 15781.0 48 23855.5 49 23739.5 50 14973.5 51 10300.0 52 8101.5 53 7032.5 54 6441.0 55 5841.0 56 5275.0 57 4734.5 58 4282.0 59 3954.5 60 3504.5 61 3074.0 62 2622.5 63 1938.5 64 1346.5 65 1085.0 66 869.0 67 691.0 68 561.5 69 448.5 70 388.5 71 341.5 72 305.0 73 271.0 74 231.0 75 184.5 76 143.0 77 98.5 78 76.0 79 70.0 80 51.5 81 33.5 82 23.0 83 15.5 84 9.5 85 6.5 86 5.5 87 3.0 88 2.5 89 3.5 90 2.5 91 3.0 92 2.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2605726990181355 2 0.16751102079737284 3 0.1932623344968168 4 0.1664911667894741 5 0.23048700578512188 6 0.19096766297904455 7 0.23456642181671697 8 0.2394107283542361 9 0.149153648655195 10-11 0.20180361181296896 12-13 0.2071578453544375 14-15 0.17044310107008181 16-17 0.2796949616662375 18-19 0.22653507150451413 20-21 0.19402722500274086 22-23 0.23737102033843854 24-25 0.2264075897535268 26-27 0.33604189560264447 28-29 0.30442642135778264 30-31 0.2812247426780856 32-33 0.18943788196719638 34-35 0.35146718747211336 36-37 0.39430105580386166 38-39 0.3764536106656332 40-41 0.3374441948635053 42-43 0.37071693187120264 44-45 0.3798956179422916 46-47 0.3839750339738866 48-49 0.4057744133927228 50-51 0.39850795358644414 52-53 0.3987629170884188 54-55 0.39187890253510216 56-57 0.3695695961123165 58-59 0.3445831729187967 60-61 0.31143791766208667 62-63 0.37976813619130423 64-65 0.4132958367009763 66-67 0.41966992425034355 68-69 0.42425926728588803 70-71 0.4218371140171285 72-73 0.4132958367009763 74-75 0.4097263476733306 76-77 0.39302623829398825 78-79 0.3657451435826961 80-81 0.3772185011715573 82-83 0.3872895594995577 84-85 0.3875445230015323 86-87 0.38626970549165884 88-89 0.394428537554849 90-91 0.413933245455913 92-93 0.4120210191911028 94-95 0.42081726000922964 96-97 0.4226020045230526 98-99 0.41763021623454605 100-101 0.39315372004497556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 392213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.35087654426116 #Duplication Level Percentage of deduplicated Percentage of total 1 84.82821792081423 43.56003345921414 2 8.254079295420375 8.477084137713478 3 2.514477459149639 3.873618647343618 4 1.1487722558652664 2.359618491536388 5 0.6672465511231197 1.7131847635653685 6 0.47322098346691094 1.4580187380097915 7 0.3192319929800674 1.147498986234852 8 0.25663711578109943 1.0542832679320397 9 0.18957600365928748 0.8761404563696226 >10 1.1862911979708843 11.333842195205552 >50 0.09107855199308008 3.138676966578322 >100 0.054248973591506156 5.419655056856882 >500 0.009456243103106578 3.4535413931579293 >1k 0.005972364065119945 6.930084246049761 >5k 0.0014930910162799862 5.204719194232312 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7672 1.9560799871498395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6787 1.7304372879022367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5906 1.5058144426625328 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4765 1.2149010869094088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4229 1.0782406498509738 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3161 0.8059396297420024 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2895 0.7381193382167343 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2432 0.6200712368024518 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1812 0.4619938655781424 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1642 0.41865007024244477 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1553 0.39595831856669716 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1497 0.3816803624561144 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.2766353996425412 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1043 0.2659269325596041 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1002 0.2554734289786417 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 901 0.22972211527919778 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 884 0.22538773574562804 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 870 0.2218182467179823 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 863 0.22003350220415946 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 854 0.21773883068638725 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 804 0.20499065558765264 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 787 0.20065627605408287 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 776 0.19785167753236121 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 753 0.1919875169869433 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 752 0.19173255348496865 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 673 0.17159043682896793 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 661 0.16853087480527162 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 598 0.152468174180866 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 595 0.15170328367494193 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 576 0.14685897713742277 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.1394650355801567 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 545 0.13895510857620733 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 541 0.13793525456830855 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 533 0.13589554655251102 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 494 0.125951969975498 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 468 0.119322918924156 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 458 0.11677328390440908 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 427 0.10886941534319362 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 426 0.10861445184121893 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 416 0.10606481682147201 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 398 0.10147547378592756 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.2748175098734616E-4 0.0 16-17 0.0 0.0 0.0 6.374087549367309E-4 0.0 18-19 0.0 0.0 0.0 8.923722569114231E-4 0.0 20-21 0.0 0.0 0.0 0.002422153268759577 0.0 22-23 0.0 0.0 0.0 0.009688613075038308 0.0 24-25 0.0 0.0 0.0 0.021161970663899462 0.0 26-27 0.0 0.0 0.0 0.0367147442843557 0.0 28-29 0.0 0.0 0.0 0.08528529141053459 0.0 30-31 0.0 0.0 0.0 0.19428218850471554 0.0 32-33 0.0 0.0 0.0 0.38078799019920295 0.0 34-35 0.0 0.0 0.0 0.6980900684067075 0.0 36-37 0.0 0.0 0.0 1.1391769268229255 0.0 38-39 0.0 0.0 0.0 1.626284697345575 0.0 40-41 0.0 0.0 0.0 2.1330246575202763 0.0 42-43 0.0 0.0 0.0 2.5895368078059624 0.0 44-45 0.0 0.0 0.0 3.108642497826436 0.0 46-47 0.0 0.0 0.0 3.7190251215538495 0.0 48-49 0.0 0.0 0.0 4.308628219870325 0.0 50-51 0.0 0.0 0.0 4.8777067562778385 0.0 52-53 0.0 0.0 0.0 5.367236680069248 0.0 54-55 0.0 0.0 0.0 5.9048272239828865 0.0 56-57 0.0 0.0 0.0 6.560848314563771 0.0 58-59 0.0 0.0 0.0 7.283414879160048 0.0 60-61 0.0 0.0 0.0 8.111663815324835 0.0 62-63 0.0 0.0 0.0 8.874642094984104 2.549635019746923E-4 64-65 0.0 0.0 0.0 9.564828294829596 2.549635019746923E-4 66-67 0.0 0.0 0.0 10.336602815306989 2.549635019746923E-4 68-69 0.0 0.0 0.0 11.151338685867119 2.549635019746923E-4 70-71 0.0 0.0 0.0 12.028540614411048 2.549635019746923E-4 72-73 0.0 0.0 0.0 12.938760316460698 2.549635019746923E-4 74-75 0.0 0.0 0.0 13.824248558818805 2.549635019746923E-4 76-77 0.0 0.0 0.0 14.678886217437974 2.549635019746923E-4 78-79 0.0 0.0 0.0 15.650297159961552 2.549635019746923E-4 80-81 0.0 0.0 0.0 16.67308324813303 2.549635019746923E-4 82-83 0.0 1.2748175098734616E-4 0.0 17.675344774395544 2.549635019746923E-4 84-85 0.0 2.549635019746923E-4 0.0 18.80355827063356 2.549635019746923E-4 86-87 0.0 2.549635019746923E-4 0.0 19.976772824970105 2.549635019746923E-4 88-89 0.0 2.549635019746923E-4 0.0 21.013454423999203 2.549635019746923E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGA 55 4.948878E-5 43.194214 1 ATGGGCG 75 6.9761536E-6 37.947742 5 ACGCAGG 60 0.003974477 31.623121 7 TAATACC 155 1.371518E-9 30.603018 4 GTCCTGA 80 4.4807297E-4 29.696022 1 CATGAGA 130 3.0649244E-7 29.190573 4 TATATGG 65 0.005878819 29.190573 2 GTCCCTA 65 0.005878819 29.190573 6 CTGGACG 65 0.005878819 29.190573 4 GTATATG 70 0.008373174 27.15065 1 GTACAGG 130 9.976744E-6 25.584265 1 CATGGGG 1095 0.0 25.12522 4 GGTTTAG 95 0.0012230561 25.00718 1 TAGGAGG 210 1.2787496E-9 24.846737 4 GGATATG 115 1.2771452E-4 24.789724 1 CTACACA 115 1.2916526E-4 24.748528 4 ATGAGAG 135 1.3541461E-5 24.59576 5 GATATAG 120 1.704679E-4 23.756819 1 ATGGGGC 225 2.8430804E-9 23.190289 5 GAGTACT 4695 0.0 22.949007 12-13 >>END_MODULE