##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938840_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392213 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.873920548273514 31.0 31.0 34.0 30.0 34.0 2 32.028385086674845 33.0 31.0 34.0 30.0 34.0 3 32.057866516408176 33.0 31.0 34.0 30.0 34.0 4 35.745273614082144 37.0 35.0 37.0 33.0 37.0 5 35.67026845107123 37.0 35.0 37.0 33.0 37.0 6 35.74486312284397 37.0 35.0 37.0 33.0 37.0 7 35.66039371463975 37.0 35.0 37.0 33.0 37.0 8 35.69116271005805 37.0 35.0 37.0 33.0 37.0 9 37.22581352479393 39.0 37.0 39.0 33.0 39.0 10-11 37.169651439396446 39.0 37.0 39.0 33.0 39.0 12-13 37.08266936588028 39.0 37.0 39.0 33.0 39.0 14-15 38.059786391578044 40.0 37.0 41.0 33.0 41.0 16-17 37.99499506645624 40.0 37.0 41.0 33.0 41.0 18-19 38.189989877948975 40.0 37.0 41.0 33.0 41.0 20-21 38.21960133906831 40.0 37.0 41.0 33.0 41.0 22-23 38.07218526667908 40.0 37.0 41.0 33.0 41.0 24-25 37.91176478087162 40.0 37.0 41.0 33.0 41.0 26-27 37.574142111556725 40.0 36.0 41.0 32.5 41.0 28-29 37.26278705703279 39.0 35.5 41.0 32.0 41.0 30-31 36.8575467921767 39.0 35.0 41.0 31.5 41.0 32-33 36.38455252630586 38.0 35.0 41.0 30.5 41.0 34-35 36.08122882209412 38.0 35.0 41.0 30.0 41.0 36-37 35.722470953283036 38.0 35.0 41.0 30.0 41.0 38-39 35.343713747377066 38.0 35.0 40.5 28.0 41.0 40-41 34.98654557600079 38.0 35.0 40.0 26.5 41.0 42-43 34.586701613664005 38.0 34.5 40.0 24.5 41.0 44-45 34.04855779895108 38.0 33.5 40.0 21.0 41.0 46-47 33.28302860945456 38.0 33.0 40.0 11.5 41.0 48-49 32.60276431428841 37.5 33.0 40.0 2.0 41.0 50-51 32.15824692195312 37.0 33.0 40.0 2.0 41.0 52-53 31.821201744970207 37.0 32.0 40.0 2.0 41.0 54-55 31.475854956362998 36.0 31.0 40.0 2.0 41.0 56-57 31.10075647671036 36.0 31.0 40.0 2.0 41.0 58-59 30.67499674921535 35.5 30.5 40.0 2.0 41.0 60-61 30.159835344570425 35.0 29.5 39.0 2.0 41.0 62-63 29.678659299921215 35.0 28.0 39.0 2.0 40.5 64-65 29.120071236802453 35.0 27.0 38.5 2.0 40.0 66-67 28.57848286517785 35.0 26.0 38.0 2.0 40.0 68-69 28.211860647148363 35.0 26.0 37.0 2.0 40.0 70-71 27.74683908998427 34.5 25.5 37.0 2.0 39.0 72-73 27.21649460879675 34.0 24.0 36.0 2.0 39.0 74-75 26.7195350485578 34.0 22.5 35.5 2.0 38.0 76-77 24.92865611287744 31.5 19.0 34.5 2.0 36.0 78-79 25.67522621636713 33.0 18.0 35.0 2.0 37.0 80-81 25.66038606573469 33.0 17.0 35.0 2.0 36.0 82-83 25.317096832588415 33.0 9.0 35.0 2.0 36.0 84-85 24.92009826293366 33.0 3.5 35.0 2.0 35.5 86-87 24.365862426793605 33.0 2.0 35.0 2.0 35.0 88-89 23.764515709576173 32.0 2.0 35.0 2.0 35.0 90-91 23.242309663371685 31.5 2.0 35.0 2.0 35.0 92-93 22.96334007286857 31.0 2.0 34.0 2.0 35.0 94-95 22.625990214500796 31.0 2.0 34.0 2.0 35.0 96-97 22.063826288266835 31.0 2.0 34.0 2.0 35.0 98-99 21.344780769632827 31.0 2.0 34.0 2.0 35.0 100-101 18.556784196342292 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1979117440547462 1101 2 0.21847363829360233 1101 3 0.024343929373323192 1101 4 0.18949596236268462 1101 5 0.012863110243536369 1101 6 0.1760859399723529 1101 7 0.4540243588379198 1101 8 0.12395294381803268 1101 9 0.4255602797704654 1101 10-11 0.4007219955222254 1101 12-13 0.4853655653076885 1101 14-15 0.41785391098108704 1101 16-17 0.38451916433279365 1101 18-19 0.5364476918446002 1101 20-21 0.515636262198818 1101 22-23 0.3986171517635242 1101 24-25 0.5941508240705673 1101 26-27 0.7250670930379286 1101 28-29 0.5234014859556595 1101 30-31 0.7874572640571813 1101 32-33 0.9521067553749063 1101 34-35 1.0688057810144898 1101 36-37 1.1654894491294598 1101 38-39 1.2858759046079484 1101 40-41 1.0980403779696317 1101 42-43 1.1067614974859836 1101 44-45 1.2621689610219704 1101 46-47 1.340975279363974 1101 48-49 0.8956835762823161 1101 50-51 0.9008260861633701 1101 52-53 0.7623658439574719 1101 54-55 0.7816085905023122 1101 56-57 1.0554879554531702 1101 58-59 0.8877072756357265 1101 60-61 0.798336420692646 1101 62-63 0.46656177888024075 1101 64-65 0.6438045109154196 1101 66-67 0.8888532030142215 1101 68-69 0.6000626720845297 1101 70-71 0.9522976540550196 1101 72-73 1.002638910683455 1101 74-75 0.943973138149218 1101 76-77 0.9688162922244246 1101 78-79 1.0703950233878672 1101 80-81 1.261819835758594 1101 82-83 1.0628430335706973 1101 84-85 1.1656226605107562 1101 86-87 1.140835175177621 1101 88-89 1.4122120584743278 1101 90-91 1.2086462544839804 1101 92-93 1.2806576239573424 1101 94-95 1.1650443690541223 1101 96-97 1.7750340260094148 1101 98-99 1.3876933357223074 1101 100-101 1.3130205353227495 1102 1 0.2868826859668623 1102 2 0.2665878334666232 1102 3 0.1535067055949213 1102 4 0.07904976368119065 1102 5 0.10034860949374291 1102 6 0.10008786397428082 1102 7 -0.15429977889797897 1102 8 0.3545360861658793 1102 9 0.2721777146231972 1102 10-11 0.2890308544193232 1102 12-13 0.11853903671645583 1102 14-15 0.10888891083961738 1102 16-17 0.20953401875942035 1102 18-19 0.4869258629110078 1102 20-21 0.4983556125652271 1102 22-23 0.17653640027101147 1102 24-25 0.19858451673955102 1102 26-27 0.19508237187673672 1102 28-29 0.4477217190994196 1102 30-31 0.4440653618417514 1102 32-33 0.3477650186802279 1102 34-35 0.391924927663041 1102 36-37 0.29312434147023936 1102 38-39 0.4975373368576186 1102 40-41 0.10568687091024032 1102 42-43 0.01009454787784847 1102 44-45 -0.022880412262693994 1102 46-47 0.26513282116857084 1102 48-49 -0.09688213687163483 1102 50-51 -0.027073356441952967 1102 52-53 0.13018974119313143 1102 54-55 0.2824432678075759 1102 56-57 0.28530715586060396 1102 58-59 0.23044133895802688 1102 60-61 0.10506618330476059 1102 62-63 0.11231199815987125 1102 64-65 -0.17866387037648934 1102 66-67 -0.025061857959663314 1102 68-69 -0.016282856025700454 1102 70-71 0.14890660066396677 1102 72-73 0.43755530148220245 1102 74-75 0.1042453278331763 1102 76-77 -0.21310091910454787 1102 78-79 0.02832162837329477 1102 80-81 0.15865230612047299 1102 82-83 -0.142013656580108 1102 84-85 -0.18439714539140084 1102 86-87 -0.0776380727073942 1102 88-89 -0.11035959645026949 1102 90-91 -0.05715602602418102 1102 92-93 -0.15194572041071197 1102 94-95 -0.2989654773086663 1102 96-97 -0.1376643866889964 1102 98-99 0.010001855089651457 1102 100-101 0.1892902351218595 1103 1 -0.198190912357191 1103 2 -0.07144072378570598 1103 3 0.053294455746531355 1103 4 -0.0636813681342332 1103 5 -0.08713416243414684 1103 6 -0.07376405730032332 1103 7 -0.11354505151499694 1103 8 -0.015467772922271195 1103 9 -0.03987090988752584 1103 10-11 0.024256059777947314 1103 12-13 0.024625370031408522 1103 14-15 -0.03670208094604277 1103 16-17 -0.14869962146330806 1103 18-19 -0.1373850142046038 1103 20-21 0.09400586891894136 1103 22-23 0.14641483899419683 1103 24-25 0.09248715745845004 1103 26-27 0.03250450099080382 1103 28-29 -0.23430319958407608 1103 30-31 0.005424863468078911 1103 32-33 0.19259521880151453 1103 34-35 0.07237175494128678 1103 36-37 0.0061115513494982565 1103 38-39 -0.04183914475657957 1103 40-41 -0.34242217589498125 1103 42-43 0.048471801309680984 1103 44-45 0.08825443530332677 1103 46-47 0.019873986613198724 1103 48-49 0.190268477825974 1103 50-51 0.2058157223385777 1103 52-53 -0.04411700118516748 1103 54-55 -0.118598685993625 1103 56-57 -0.1039990352193918 1103 58-59 -0.04194826355589498 1103 60-61 -0.034246899004074294 1103 62-63 0.17525648242030556 1103 64-65 0.2456290895441242 1103 66-67 0.13243717726830084 1103 68-69 0.21806027204089418 1103 70-71 0.038433311066967235 1103 72-73 0.3173378142998864 1103 74-75 0.15697701094690686 1103 76-77 0.15204462364863502 1103 78-79 0.0918863232296836 1103 80-81 0.29587045486075425 1103 82-83 0.1396239750423618 1103 84-85 0.0819075020533333 1103 86-87 -0.4357821269551465 1103 88-89 -0.3613588246326387 1103 90-91 -0.4246652602708352 1103 92-93 -0.4552080281454245 1103 94-95 -0.06214839808709627 1103 96-97 -0.20596335576115976 1103 98-99 0.0021045068345131313 1103 100-101 -0.2625262928013967 1104 1 -0.001079658061655664 1104 2 -0.11093277705031213 1104 3 -0.12268814734625266 1104 4 0.09954887241559618 1104 5 0.02307309701602378 1104 6 0.1933643791257964 1104 7 0.0016020704781354311 1104 8 -0.14650836570577752 1104 9 0.07933012104030723 1104 10-11 -0.061104228022749396 1104 12-13 -0.06401026141188737 1104 14-15 -0.11058524245807178 1104 16-17 0.11411985877723652 1104 18-19 0.13679078046893522 1104 20-21 0.06236658627288705 1104 22-23 0.039469499647118766 1104 24-25 -0.034982773813034385 1104 26-27 -0.0677156450573122 1104 28-29 -0.2234569796528092 1104 30-31 -0.6392540454666289 1104 32-33 -0.4690241970060427 1104 34-35 -0.5061156806257259 1104 36-37 -0.05483131806630581 1104 38-39 -0.08519717740263388 1104 40-41 -0.007531711977456723 1104 42-43 0.012308785845768 1104 44-45 0.23589994647170442 1104 46-47 0.23172428730048722 1104 48-49 -0.1640055771568356 1104 50-51 0.0891114697612565 1104 52-53 -0.48073104757692064 1104 54-55 -0.7482566542066813 1104 56-57 -0.6539614493087349 1104 58-59 -0.6644380616658587 1104 60-61 -0.817094300206822 1104 62-63 -0.4813962237652625 1104 64-65 -0.4423810049575927 1104 66-67 -0.74863026259424 1104 68-69 -0.7677241996085904 1104 70-71 -0.5048576038814865 1104 72-73 -0.40813737899908276 1104 74-75 -0.3097057639671803 1104 76-77 -0.4685390318496516 1104 78-79 -0.3108467094163636 1104 80-81 -0.3094640597440552 1104 82-83 -0.16717102066210643 1104 84-85 0.369037012362611 1104 86-87 0.3896984357596338 1104 88-89 -0.005984039409273834 1104 90-91 -0.01696551800279167 1104 92-93 -0.17848176096329738 1104 94-95 0.10986083730809071 1104 96-97 0.3987939466443393 1104 98-99 0.6666905248757473 1104 100-101 1.3166994374391514 1105 1 0.17481395445949133 1105 2 0.09162380078387145 1105 3 -0.0549137184122408 1105 4 0.2256064020264219 1105 5 0.07154761055240755 1105 6 0.08393650264525121 1105 7 0.20288961277931605 1105 8 0.3296575606901584 1105 9 0.3226676844920817 1105 10-11 0.12176247036980925 1105 12-13 0.03047339975562835 1105 14-15 0.16204589121367974 1105 16-17 0.10349829574847291 1105 18-19 0.03920062606961494 1105 20-21 -0.04184634731424808 1105 22-23 -0.19216253292479024 1105 24-25 0.02376299606852683 1105 26-27 0.12191263328448798 1105 28-29 0.38055105757223373 1105 30-31 0.12302651713912383 1105 32-33 0.28356675054048 1105 34-35 0.28483054399356433 1105 36-37 0.3344188934396186 1105 38-39 0.3540345273351022 1105 40-41 0.31900699360630114 1105 42-43 0.27202483914526 1105 44-45 0.2556559215140055 1105 46-47 0.33579842012620276 1105 48-49 0.08826811150644431 1105 50-51 -0.036642020086461 1105 52-53 0.19062840673779746 1105 54-55 0.26574900357673314 1105 56-57 0.23744347877925165 1105 58-59 0.4480368220058786 1105 60-61 0.4750680834649188 1105 62-63 0.5933579006002212 1105 64-65 0.5598265881050111 1105 66-67 0.938896175646029 1105 68-69 0.5113644742492767 1105 70-71 0.3720261207378037 1105 72-73 0.37031677709228816 1105 74-75 0.4293355854406009 1105 76-77 0.3511719981841921 1105 78-79 -0.010970969145635223 1105 80-81 -0.3489958385088592 1105 82-83 0.1744308723158987 1105 84-85 0.1583374565250466 1105 86-87 0.02112953770548387 1105 88-89 0.14766179392406187 1105 90-91 0.32956049722563563 1105 92-93 0.5552124543497321 1105 94-95 0.7796604354468393 1105 96-97 0.729086882346536 1105 98-99 0.806542206347828 1105 100-101 1.1876411675575937 1106 1 0.2895296777779457 1106 2 0.06252821919029117 1106 3 0.3360955305986124 1106 4 0.32636591502210877 1106 5 0.25778096042454735 1106 6 0.17664288789772087 1106 7 0.13684251534029102 1106 8 0.14901560045963436 1106 9 0.44352185036361647 1106 10-11 0.15587376383189167 1106 12-13 0.29461090086881114 1106 14-15 0.3032618753364176 1106 16-17 0.5570337842158395 1106 18-19 0.19650060189751173 1106 20-21 0.4380812635911866 1106 22-23 0.5597839576671717 1106 24-25 0.5520316372145402 1106 26-27 0.47757335870209516 1106 28-29 0.5615524188434335 1106 30-31 0.7135224269644524 1106 32-33 0.5379463543724015 1106 34-35 0.5701981508279061 1106 36-37 0.615085661883576 1106 38-39 0.686948029364288 1106 40-41 0.5519529371453515 1106 42-43 0.7948706592793471 1106 44-45 0.510776591208554 1106 46-47 0.7561799577265518 1106 48-49 0.7917431697103225 1106 50-51 1.1102385061021138 1106 52-53 0.8188264398917546 1106 54-55 1.001185310079034 1106 56-57 0.9364403364055498 1106 58-59 1.2308568161536861 1106 60-61 1.1338279272367835 1106 62-63 0.9520115143299783 1106 64-65 1.0527435251175952 1106 66-67 1.4100868426689175 1106 68-69 1.3411515052786278 1106 70-71 1.3697997426097395 1106 72-73 1.2117032381688375 1106 74-75 1.3025083651054992 1106 76-77 1.2662961028621282 1106 78-79 1.0161622191550599 1106 80-81 0.8970189446419106 1106 82-83 1.0380588508917796 1106 84-85 1.3884018306583492 1106 86-87 1.4891364840052432 1106 88-89 1.2541780846508779 1106 90-91 1.2262597326237739 1106 92-93 1.1820778411670325 1106 94-95 1.006731921267992 1106 96-97 1.3598293476468442 1106 98-99 1.5580497823945443 1106 100-101 2.094836185559455 1107 1 -0.02928716631248207 1107 2 -0.07228281830443706 1107 3 -0.20831624140565808 1107 4 -0.1372498074523918 1107 5 -0.44178354489816485 1107 6 -0.2849256373758564 1107 7 0.06500450447153128 1107 8 0.15106279766055764 1107 9 -0.07475403737553421 1107 10-11 -0.2415523509600419 1107 12-13 -0.2089282688376315 1107 14-15 -0.22238392232605264 1107 16-17 -0.5241717047541101 1107 18-19 -0.15256823355746718 1107 20-21 -0.6186229970604415 1107 22-23 -0.3170306034881847 1107 24-25 -0.2805479553311869 1107 26-27 -0.3859654387866769 1107 28-29 -0.41539179813464955 1107 30-31 -0.3430236411761953 1107 32-33 0.15855418583223724 1107 34-35 0.19627448026536598 1107 36-37 -0.003521499584458354 1107 38-39 -0.0019927632112057836 1107 40-41 -0.30378790009627465 1107 42-43 -0.4910173280156229 1107 44-45 -0.12036810220816108 1107 46-47 -0.02938854273251934 1107 48-49 0.07310250865173629 1107 50-51 -0.3170611787535975 1107 52-53 -0.6180299337155333 1107 54-55 -0.3904931434806116 1107 56-57 -0.422706292543058 1107 58-59 -0.11337318955365205 1107 60-61 -0.18906470037184064 1107 62-63 -0.14499461485437237 1107 64-65 -0.34277807591469056 1107 66-67 -0.09696978404638656 1107 68-69 0.03530024346071414 1107 70-71 -0.02469825982208107 1107 72-73 -0.1125386785040341 1107 74-75 0.14416696705591647 1107 76-77 0.11916466039457418 1107 78-79 -0.08361382987841282 1107 80-81 0.2223402925661766 1107 82-83 0.22931980029499144 1107 84-85 0.4692071856299407 1107 86-87 0.35645018905996295 1107 88-89 0.1934538731319826 1107 90-91 0.27403076912592184 1107 92-93 0.10035745108291039 1107 94-95 0.5685293604104018 1107 96-97 0.4650583855882289 1107 98-99 0.9023418243806987 1107 100-101 1.4771006338087886 1108 1 -0.006509622600663789 1108 2 0.07883894280784176 1108 3 0.022092437760129258 1108 4 0.008236815678003495 1108 5 -0.3106480909272378 1108 6 -0.3351152308032823 1108 7 -0.25277514583392247 1108 8 -0.05791129123769423 1108 9 -0.2225315810873525 1108 10-11 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-0.2646785412351669 2316 56-57 -0.21032090850811969 2316 58-59 -0.12241956185831881 2316 60-61 -0.6023167068657926 2316 62-63 -0.4292707907398601 2316 64-65 -0.5841547251269574 2316 66-67 -0.36216711786752853 2316 68-69 -0.8628544680843397 2316 70-71 -0.7513663365604693 2316 72-73 -0.9648727283062435 2316 74-75 -1.2117999229356187 2316 76-77 -0.7597997371999199 2316 78-79 -0.6106386166527038 2316 80-81 -0.42486833707276617 2316 82-83 -0.5489340354811958 2316 84-85 -0.8428026238960484 2316 86-87 -1.344156332331206 2316 88-89 -1.321160376976632 2316 90-91 -1.7052954833607572 2316 92-93 -1.4481814097315784 2316 94-95 -1.4506123632307855 2316 96-97 -1.436740599160128 2316 98-99 -1.4820446800896079 2316 100-101 -1.3272033243006476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 22.0 5 59.0 6 185.0 7 314.0 8 576.0 9 902.0 10 1574.0 11 2390.0 12 3333.0 13 4457.0 14 5027.0 15 6535.0 16 7836.0 17 10294.0 18 9569.0 19 5887.0 20 5235.0 21 5547.0 22 5454.0 23 5660.0 24 6017.0 25 6418.0 26 6545.0 27 6965.0 28 8077.0 29 9360.0 30 10762.0 31 12547.0 32 14872.0 33 17908.0 34 23881.0 35 35663.0 36 42512.0 37 61008.0 38 45246.0 39 3570.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.364473657309915 21.381074560955167 15.906303027824913 17.348148753910003 2 15.171603185004068 24.99254231756724 38.441867046732256 21.393987450696432 3 20.646944389910583 30.785823009436196 32.53385277897469 16.033379821678526 4 11.692881164061365 20.40651380754845 37.32640172559299 30.574203302797205 5 10.521805880357748 42.473840849334 32.77474440157197 14.229608868736277 6 23.99653229302124 38.583594686249015 18.852851934011575 18.56702108671817 7 22.04975357777534 39.89796360650973 21.056160810579964 16.996122005134964 8 24.584600714407735 32.4410460642559 21.069163949180673 21.90518927215569 9 25.878030559925346 19.212009800797016 22.789402697003926 32.120556942273716 10-11 22.76033685777881 29.073488130174162 26.227075594128703 21.939099417918325 12-13 27.5619370087172 27.933418831094333 26.90744569914817 17.597198461040303 14-15 23.91175203269652 28.346714667795304 29.801281446560925 17.94025185294725 16-17 17.550978779823943 32.09377928212189 30.83535718611159 19.51988475194258 18-19 16.769969378883413 30.197112283376637 35.4896446573673 17.543273680372653 20-21 19.043988852995692 28.306302952732317 34.27168400843419 18.378024185837795 22-23 18.43424363802322 28.868497474586512 33.97987828042416 18.717380606966113 24-25 18.642771365413005 29.72303173546892 34.63645843691278 16.99773846220529 26-27 18.954786630529284 28.813228728707035 35.940460012187444 16.291524628576237 28-29 16.792278685306197 29.997220897828473 36.57196472324987 16.63853569361546 30-31 17.341597550310674 30.88704351972015 35.28694867329742 16.484410256671758 32-33 16.87042499866144 31.055064467521476 34.8821941139126 17.19231641990449 34-35 17.752231411491277 29.399173093276254 34.84877653247388 17.9998189627586 36-37 17.97009680413738 29.831663827486665 35.374185223343154 16.824054145032797 38-39 18.088405172523707 29.061657686236607 35.731316412894074 17.118620728345604 40-41 16.59711836725327 30.012736709645093 36.35508263561918 17.035062287482454 42-43 16.397591390611115 31.040233160324497 34.62433368649065 17.93784176257374 44-45 16.31199706269314 31.18606513069996 33.63560566655448 18.86633214005242 46-47 17.500761706375258 30.691731374526242 31.98835322268725 19.81915369641125 48-49 17.586826293868903 30.37693250094657 32.07247221163395 19.963768993550573 50-51 17.41375222136951 30.98622432198831 32.58229584434988 19.0177276122923 52-53 16.72343853977303 31.56524643497283 32.516515260840414 19.194799764413727 54-55 15.710723509929553 30.882071731431644 32.324145298600506 21.083059460038296 56-57 17.444713999242754 31.622814789507164 30.004933575210252 20.92753763603983 58-59 17.31091858484697 30.595188316522698 30.889544430053135 21.204348668577197 60-61 17.253613599218763 30.702780986183036 30.992684836014373 21.05092057858383 62-63 18.065368209296572 30.786391949190357 30.745852338931538 20.402387502581533 64-65 17.603399238409455 31.113791112057328 31.12003783730723 20.162771812225987 66-67 16.78665451277115 30.92703200596646 30.85908068104311 21.427232800219283 68-69 16.496903468268517 31.492964282163012 30.6588511854528 21.35128106411567 70-71 17.17370408426034 31.02587114654537 29.74060013309095 22.059824636103343 72-73 17.08331952281031 29.939216701129233 30.05191056900205 22.92555320705841 74-75 16.811847689320686 30.93726216594873 29.885916144733134 22.36497399999745 76-77 17.32004342097039 29.532127563769766 30.756308095000595 22.39152092025925 78-79 16.722233851903663 29.472605292582934 31.24926605364063 22.555894801872775 80-81 16.662077265124626 30.389487210867706 30.320263737608617 22.628171786399054 82-83 16.656319279158915 30.141366135614096 29.913043699970803 23.289270885256183 84-85 16.97470507096909 29.137356487418824 29.684635644407503 24.20330279720458 86-87 17.06532114400053 29.477333864192047 29.60673067597711 23.85061431583031 88-89 17.366878702255217 28.772159168673824 29.464930985038123 24.396031144032836 90-91 16.735190054831506 28.647382867233535 29.98355694607062 24.633870131864334 92-93 16.869660108155518 29.49787487921104 29.220602070813563 24.41186294181988 94-95 16.766825084393133 29.324829428982284 28.900059151683273 25.00828633494131 96-97 16.62017832147328 28.799657329053346 28.540486929296073 26.039677420177306 98-99 17.083447004561297 28.886217437973755 28.060518136828712 25.969817420636236 100-101 17.413525169584112 28.530033056313016 28.863528292914886 25.19291348118799 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5754.0 1 3625.5 2 1612.5 3 1652.0 4 1371.5 5 1314.0 6 2007.5 7 3253.0 8 4122.5 9 4140.0 10 3335.0 11 2419.0 12 2134.5 13 2062.5 14 1913.5 15 1709.0 16 1596.0 17 1598.5 18 1624.0 19 1689.0 20 1795.0 21 1960.0 22 2187.0 23 2403.5 24 2691.0 25 3125.0 26 3714.0 27 4361.5 28 4873.0 29 5434.0 30 6146.0 31 6803.0 32 7594.0 33 8369.0 34 9117.0 35 9847.0 36 10492.0 37 11063.0 38 10878.5 39 10099.0 40 9718.5 41 9648.0 42 9478.5 43 9693.0 44 10244.0 45 10668.5 46 11945.5 47 15938.0 48 23547.0 49 23873.0 50 17269.0 51 12527.0 52 8464.0 53 6540.0 54 5867.5 55 5423.5 56 5075.5 57 4477.0 58 3938.5 59 3629.5 60 3203.5 61 2794.0 62 2360.0 63 1688.0 64 1117.0 65 864.0 66 650.0 67 485.5 68 369.5 69 282.0 70 254.0 71 230.0 72 187.0 73 186.5 74 175.0 75 116.0 76 79.0 77 56.5 78 44.0 79 33.0 80 19.5 81 14.0 82 13.0 83 9.0 84 3.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2317618232949953 2 0.0 3 0.0 4 0.0 5 0.02396656918562108 6 0.005864160545417924 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003951934280607731 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.099270039493846E-4 26-27 0.001529781011848154 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0072664598062787316 36-37 0.020652043659950076 38-39 0.016317664126380308 40-41 0.010071058328000346 42-43 0.009306167822076269 44-45 0.004334379533569769 46-47 3.824452529620385E-4 48-49 0.0019122262648101924 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.374087549367309E-4 58-59 2.549635019746923E-4 60-61 0.0035694890276456925 62-63 0.0010198540078987693 64-65 0.0019122262648101924 66-67 0.005226751790481193 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.824452529620385E-4 76-77 0.06029886821701474 78-79 0.1262069334774727 80-81 0.0012748175098734617 82-83 0.0011473357588861154 84-85 0.0 86-87 0.10071058328000347 88-89 0.3364243408556065 90-91 0.37492382965378507 92-93 0.0 94-95 2.549635019746923E-4 96-97 0.0 98-99 0.0 100-101 8.923722569114231E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 392213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11000636320768 #Duplication Level Percentage of deduplicated Percentage of total 1 83.24745313086655 40.05035499846822 2 9.10694861289703 8.76270711431763 3 2.7140788164645415 3.917230473910687 4 1.3075450366915664 2.516240001416475 5 0.7914300093627493 1.9037851393237695 6 0.4889013886402068 1.4112629351076855 7 0.3292360918541369 1.1087685331870105 8 0.26337208736291323 1.0136666239136836 9 0.2125578880410193 0.9203545211583082 >10 1.3541947486259043 12.180148865979143 >50 0.09665521162303706 3.132418444762393 >100 0.06691514650825638 6.0737235945262285 >500 0.012214669600713466 4.318956067721326 >1k 0.006903943687359785 7.661650236793624 >5k 0.001593217774006104 5.028732449413816 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6933 1.7676619591905418 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6848 1.7459900615226929 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5901 1.5045396251526593 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4529 1.1547297004433816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 1.096343058491177 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3172 0.808744228263724 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2937 0.7488278052996714 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2644 0.6741234992210865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2467 0.6289949593715659 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1715 0.4372624058865973 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1666 0.42476919428983745 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1646 0.41966992425034355 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1360 0.34675036268558157 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1303 0.3322174430730241 RNA PCR Primer, Index 33 (95% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1167 0.2975424068044659 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1081 0.2756155456346424 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 996 0.25394364796679353 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 928 0.23660612983251444 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 927 0.2363511663305398 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 918 0.23405649481276755 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 888 0.2264075897535268 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 878 0.22385795473377984 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 872 0.2223281737219317 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 790 0.20142116656000694 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 778 0.19836160453631063 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 744 0.1896928454691711 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 739 0.18841802795929763 RNA PCR Primer, Index 33 (95% over 24bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 728 0.185613429437576 RNA PCR Primer, Index 33 (96% over 29bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 710 0.18102408640203155 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 678 0.1728652543388414 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 669 0.17057058282106918 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 659 0.16802094780132223 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 655 0.16700109379342348 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 638 0.16266671425985368 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 599 0.1527231376828407 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 561 0.14303452460780242 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 517 0.13181613052091593 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 517 0.13181613052091593 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 515 0.13130620351696654 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.12187255394390294 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 470 0.11983284592810539 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 443 0.1129488313747887 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 440 0.11218394086886462 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 434 0.11065415985701647 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 426 0.10861445184121893 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.374087549367309E-4 0.0 18-19 0.0 0.0 0.0 0.0012748175098734617 0.0 20-21 0.0 0.0 0.0 0.0029320802727089614 0.0 22-23 0.0 0.0 0.0 0.009943576577013 0.0 24-25 0.0 0.0 0.0 0.021799379418836196 0.0 26-27 0.0 0.0 0.0 0.03862697054916589 0.0 28-29 0.0 0.0 0.0 0.08770744467929416 0.0 30-31 0.0 0.0 0.0 0.20014634905013345 0.0 32-33 0.0 0.0 0.0 0.3908590485272033 0.0 34-35 0.0 0.0 0.0 0.7163199587978981 0.0 36-37 0.0 0.0 0.0 1.164035868265458 0.0 38-39 0.0 0.0 0.0 1.6577726898394496 0.0 40-41 0.0 0.0 0.0 2.172671482077341 0.0 42-43 0.0 0.0 0.0 2.636195128667331 0.0 44-45 0.0 0.0 0.0 3.1676665485335773 0.0 46-47 0.0 0.0 0.0 3.788885121094915 0.0 48-49 0.0 0.0 0.0 4.3856271974666825 0.0 50-51 0.0 0.0 0.0 4.96362945644331 0.0 52-53 0.0 0.0 0.0 5.461318212297909 0.0 54-55 0.0 0.0 0.0 6.006940106523752 0.0 56-57 0.0 0.0 0.0 6.669462766404989 0.0 58-59 0.0 0.0 0.0 7.399040827305571 0.0 60-61 0.0 0.0 0.0 8.237870748802308 0.0 62-63 0.0 0.0 0.0 9.013087276556362 0.0 64-65 0.0 0.0 0.0 9.712069717219979 0.0 66-67 0.0 0.0 0.0 10.49021832524674 0.0 68-69 0.0 0.0 0.0 11.320634451178314 0.0 70-71 0.0 0.0 0.0 12.210712036571966 0.0 72-73 0.0 0.0 0.0 13.13278754146344 0.0 74-75 0.0 0.0 0.0 14.02911173265547 0.0 76-77 0.0 0.0 0.0 14.894967785361526 0.0 78-79 0.0 0.0 0.0 15.876577267964091 0.0 80-81 0.0 0.0 0.0 16.903187808665187 0.0 82-83 0.0 0.0 0.0 17.913225721737934 0.0 84-85 0.0 0.0 0.0 19.049215604786177 0.0 86-87 0.0 0.0 0.0 20.23199129044677 0.0 88-89 0.0 0.0 0.0 21.27823402079992 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATG 80 1.0600368E-5 35.701775 1 AGGACGG 35 2.0637613E-4 33.897655 26-27 ATAAGGC 75 3.0820255E-4 31.653954 3 CTTAGCT 95 3.494603E-5 29.987957 4 ATGGGTT 80 4.4988663E-4 29.675581 5 TATAAGG 80 4.4988663E-4 29.675581 2 ATACTTA 80 4.512329E-4 29.660448 6 GTATATG 65 0.0057783127 29.293764 1 TAAGGCA 65 0.0058509046 29.219036 4 CATATAG 100 4.8728318E-5 28.56142 1 TCTTAGC 85 6.414635E-4 27.929958 3 TAGCTGA 85 6.433801E-4 27.915716 6 CTATACA 105 6.79408E-5 27.201353 1 ATGGGCG 90 8.957963E-4 26.378296 5 GGGAGCG 110 9.519845E-5 25.885483 7 TAGAGCA 100 0.0016555735 23.740465 4 TCGGGAG 160 1.8608407E-6 23.740465 3 GAGTGGG 200 2.0650987E-8 23.72836 7 ATGCCGC 80 1.8730952E-6 23.72836 94-95 AGTGTAA 100 0.0016604973 23.72836 8 >>END_MODULE