FastQCFastQC Report
Thu 26 May 2016
SRR938839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674182
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54770.8123919060431753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43350.643001444713742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30110.4466153056593027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24800.3678531909781038No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.19416122056062013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.14610298109412592No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.12355714035675826No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.11035595729343116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.040.6463131
GTATCAA49000.035.401481
GACTGCG804.585788E-429.5825757
TATCAAC61050.028.0888182
ATCAACG60900.028.080223
TCAACGC61450.027.8288924
CAACGCA62250.027.3951535
AACGCAG62650.027.1980276
TAAGGCG2104.3655746E-1127.069025
ACGCAGA69950.024.156647
CGCAGAG70550.023.7499248
GAGTACT42650.023.41809812-13
GTACATG34700.023.3562451
TACATGG34500.022.9302062
GCAGAGT73250.022.5530859
ACATGGG34550.022.348593
GTATAGG1703.2065236E-622.3037281
TAAGACT3450.021.9690594
GTACTTT46350.021.49842614-15
AGTACTT42700.021.11812212-13
Produced by FastQC (version 0.11.5)