##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 674182 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56136028550154 33.0 31.0 34.0 30.0 34.0 2 31.59888724409729 33.0 31.0 34.0 30.0 34.0 3 31.5857453921938 33.0 31.0 34.0 28.0 34.0 4 35.16146826821244 37.0 35.0 37.0 33.0 37.0 5 35.206251427656035 37.0 35.0 37.0 33.0 37.0 6 35.30155358642028 37.0 35.0 37.0 33.0 37.0 7 35.22914880551542 37.0 35.0 37.0 33.0 37.0 8 35.20623807814507 37.0 35.0 37.0 32.0 37.0 9 36.77030831437208 39.0 37.0 39.0 33.0 39.0 10-11 36.67956353032267 39.0 37.0 39.0 32.0 39.0 12-13 36.56941226553067 39.0 37.0 39.0 32.0 39.0 14-15 37.69543684049708 40.0 37.5 41.0 32.0 41.0 16-17 37.62943537501743 40.0 37.0 41.0 32.0 41.0 18-19 37.607824000047465 40.0 37.0 41.0 32.0 41.0 20-21 37.5191498734763 40.0 37.0 41.0 32.0 41.0 22-23 37.39368671960984 40.0 37.0 41.0 32.0 41.0 24-25 37.353500241774476 40.0 37.0 41.0 32.0 41.0 26-27 37.228758703139505 40.0 37.0 41.0 31.0 41.0 28-29 37.07622719087724 40.0 36.0 41.0 31.0 41.0 30-31 36.79182327620731 39.0 36.0 41.0 30.5 41.0 32-33 36.49336677633043 39.0 35.0 41.0 30.0 41.0 34-35 36.10397340777416 39.0 35.0 41.0 29.5 41.0 36-37 35.81186385872064 39.0 35.0 41.0 29.0 41.0 38-39 35.58934011884031 39.0 35.0 41.0 27.5 41.0 40-41 35.32935245972156 38.5 35.0 40.0 26.5 41.0 42-43 34.97727468250413 38.0 35.0 40.0 25.0 41.0 44-45 34.77651509533034 38.5 35.0 40.5 24.0 41.0 46-47 34.41881346580004 38.0 34.0 41.0 21.5 41.0 48-49 34.062122690905426 38.0 34.0 41.0 17.5 41.0 50-51 32.856830203120225 37.0 32.5 39.5 13.0 40.5 52-53 32.91745404060031 37.5 33.0 39.5 11.0 40.5 54-55 33.400682604993904 38.0 33.0 40.0 8.5 41.0 56-57 33.280525140095705 38.0 33.0 40.0 7.5 41.0 58-59 33.050212702208015 38.0 33.0 40.0 4.0 41.0 60-61 32.76098293932499 37.0 33.0 40.0 2.0 41.0 62-63 32.38449113147489 36.5 32.5 40.0 2.0 41.0 64-65 31.977693708820468 36.0 32.0 39.5 2.0 41.0 66-67 31.57133904494632 35.5 31.0 39.0 2.0 41.0 68-69 31.152755487390646 35.0 31.0 39.0 2.0 40.5 70-71 30.734151905568524 35.0 31.0 38.0 2.0 40.0 72-73 30.29412977504591 35.0 30.0 37.0 2.0 40.0 74-75 29.826311737780003 35.0 29.5 37.0 2.0 39.0 76-77 29.366073997822546 35.0 29.0 36.0 2.0 39.0 78-79 28.915369291971604 34.0 29.0 36.0 2.0 38.5 80-81 28.451711110649647 34.0 28.5 35.0 2.0 37.0 82-83 27.962795654585854 34.0 27.0 35.0 2.0 37.0 84-85 27.5172067779917 34.0 26.0 35.0 2.0 36.5 86-87 27.091071847068 34.0 26.0 35.0 2.0 36.0 88-89 26.683233904197976 34.0 25.0 35.0 2.0 36.0 90-91 26.23364239923344 33.5 24.0 35.0 2.0 35.0 92-93 25.742039241629115 33.0 21.0 35.0 2.0 35.0 94-95 25.204708817500318 33.0 17.0 35.0 2.0 35.0 96-97 24.604009006470065 33.0 4.5 35.0 2.0 35.0 98-99 23.916888911302884 32.0 2.0 35.0 2.0 35.0 100-101 21.926084499437838 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3201169802842756 1101 2 0.10346746611512003 1101 3 0.18374135644202738 1101 4 0.4691875312622855 1101 5 0.24072024323925945 1101 6 0.13957368743645304 1101 7 0.18917841540613267 1101 8 0.3542422896011814 1101 9 0.5439451979512171 1101 10-11 0.45426121480394244 1101 12-13 0.47144911116527055 1101 14-15 0.3717252282234327 1101 16-17 0.35218205040980877 1101 18-19 0.40638458595309856 1101 20-21 0.6548646960234166 1101 22-23 0.44950967428940203 1101 24-25 0.4871204618750582 1101 26-27 0.47010159939983254 1101 28-29 0.10486266043916004 1101 30-31 0.3371777413983068 1101 32-33 0.09580334532858359 1101 34-35 0.35750994082415843 1101 36-37 0.05206709617548455 1101 38-39 -0.43177274120802167 1101 40-41 -0.4003964969831557 1101 42-43 -0.05369822698164484 1101 44-45 0.009028809216125921 1101 46-47 0.018438267554060417 1101 48-49 -0.2501135056263948 1101 50-51 -0.301326331967104 1101 52-53 -0.3394780205203105 1101 54-55 -0.4198454048256792 1101 56-57 -0.6253804893930166 1101 58-59 -0.5701882583896705 1101 60-61 -0.7319996217710383 1101 62-63 -0.568991883013986 1101 64-65 -0.5701967144809785 1101 66-67 -0.5491474901243656 1101 68-69 -0.6693090002612543 1101 70-71 -0.706534765489959 1101 72-73 -0.9131133273067782 1101 74-75 -1.0376494133079603 1101 76-77 -0.7555512304453202 1101 78-79 -0.6950794801637521 1101 80-81 -1.0257039321723838 1101 82-83 -1.2490551704273791 1101 84-85 -1.4765608737445426 1101 86-87 -1.48287684888232 1101 88-89 -1.4717202622345766 1101 90-91 -1.679461174035346 1101 92-93 -1.5504182496263113 1101 94-95 -1.493932648967892 1101 96-97 -1.5701357875787032 1101 98-99 -1.7118260238007679 1101 100-101 -1.2221062991904823 1102 1 0.21007424524153961 1102 2 0.2276504726510673 1102 3 0.1910942976184984 1102 4 -0.11009225757632635 1102 5 0.012276301560021352 1102 6 0.04125166632031352 1102 7 0.22270657025781304 1102 8 0.189555260943564 1102 9 0.37382202047509594 1102 10-11 0.20038049584969286 1102 12-13 0.11916781403103016 1102 14-15 0.26015848614198234 1102 16-17 0.33419248278788416 1102 18-19 0.41878089062880974 1102 20-21 0.2678297538414185 1102 22-23 0.14308999103148068 1102 24-25 0.13249816423805783 1102 26-27 0.2947936557095332 1102 28-29 0.38762095606509916 1102 30-31 0.21593276905039716 1102 32-33 -0.18643647367594696 1102 34-35 -0.0029255795378375637 1102 36-37 0.012411490343403386 1102 38-39 0.03645878217056975 1102 40-41 -0.023463993212416767 1102 42-43 -0.22507831747938667 1102 44-45 -0.037257012804985834 1102 46-47 -0.05000064647308733 1102 48-49 -4.5915670734331115E-4 1102 50-51 -0.05773658837735951 1102 52-53 -0.2832311628028634 1102 54-55 -0.16539907501169893 1102 56-57 -0.3246954717962325 1102 58-59 -0.31319667970691967 1102 60-61 -0.3360877313738584 1102 62-63 -0.4153499775338503 1102 64-65 -0.6249038537469431 1102 66-67 -0.48477783200470626 1102 68-69 -0.3706350434990604 1102 70-71 -0.3519124678529515 1102 72-73 -0.3851627239885289 1102 74-75 -0.606921032211936 1102 76-77 -0.5754198830345629 1102 78-79 -0.7518919487409228 1102 80-81 -0.5304330674162259 1102 82-83 -0.6466324705782078 1102 84-85 -1.1368015725881833 1102 86-87 -1.3663924346037852 1102 88-89 -1.0436313984839494 1102 90-91 -0.8199369156140293 1102 92-93 -0.9618844386660292 1102 94-95 -1.1304491145284743 1102 96-97 -0.992804213923602 1102 98-99 -0.9764376376770869 1102 100-101 -0.7514552182452867 1103 1 0.09362809139538797 1103 2 0.43751713931773395 1103 3 0.2538276309518288 1103 4 0.5138385116544413 1103 5 0.4743019425856616 1103 6 0.3830952560639034 1103 7 0.19477580102704906 1103 8 0.21611679940510697 1103 9 0.4023399691930436 1103 10-11 0.4725304958496892 1103 12-13 0.5428216601848774 1103 14-15 0.5761879733214741 1103 16-17 0.6653912007365932 1103 18-19 0.5667257624236868 1103 20-21 0.4433143692260302 1103 22-23 0.8014194782109669 1103 24-25 0.5260943180842119 1103 26-27 0.3978885275044135 1103 28-29 0.4636273663215107 1103 30-31 0.3662391793068025 1103 32-33 0.300199423759949 1103 34-35 0.43606288200063403 1103 36-37 0.32864225957417403 1103 38-39 0.4969998078115907 1103 40-41 0.64156036576194 1103 42-43 0.6216037338026652 1103 44-45 0.7206327307847644 1103 46-47 0.7156814048089615 1103 48-49 0.5497126381644506 1103 50-51 0.7296736680328948 1103 52-53 0.6548252474535445 1103 54-55 0.8580970788344544 1103 56-57 0.7856404256396701 1103 58-59 0.8119071664469217 1103 60-61 0.8854853455492204 1103 62-63 0.8429872019533349 1103 64-65 0.5664320436889554 1103 66-67 0.27740806543119234 1103 68-69 0.23760341803940221 1103 70-71 0.26895804496755815 1103 72-73 0.3148513785755753 1103 74-75 0.5310264036855017 1103 76-77 0.4016121682474889 1103 78-79 0.34053882048984363 1103 80-81 0.32078103514787415 1103 82-83 0.24072522172948752 1103 84-85 0.4912561197195089 1103 86-87 0.1507126936013421 1103 88-89 -0.1807660138685634 1103 90-91 0.29286693053981594 1103 92-93 0.3287399203083261 1103 94-95 -0.13392219145154982 1103 96-97 -0.3691606241800116 1103 98-99 -0.05329661203606051 1103 100-101 -0.07585009004015575 1104 1 0.3819973221646187 1104 2 0.48354790854850194 1104 3 0.19019686172106276 1104 4 0.10817697319290431 1104 5 0.12291732720105131 1104 6 0.24272602529467235 1104 7 0.1552065702578176 1104 8 0.20128603017433733 1104 9 0.3283091999622769 1104 10-11 0.3472074189266152 1104 12-13 0.3235908909541081 1104 14-15 0.3539725887060854 1104 16-17 0.39723735458275655 1104 18-19 0.2989411470390735 1104 20-21 0.1895605230721813 1104 22-23 0.2505579397494344 1104 24-25 0.5124020103919023 1104 26-27 0.5060116044274849 1104 28-29 0.5470119817061274 1104 30-31 0.5305161023837286 1104 32-33 0.48715326991379726 1104 34-35 0.5287090358467808 1104 36-37 0.47894995188186584 1104 38-39 0.703061346273131 1104 40-41 0.4690680580696309 1104 42-43 0.8019729645718954 1104 44-45 0.685371192323224 1104 46-47 0.5582044817320337 1104 48-49 0.35229725354906805 1104 50-51 0.425981360340586 1104 52-53 0.6781790936073904 1104 54-55 0.7327740019113804 1104 56-57 0.8775481179473559 1104 58-59 0.7305533202930761 1104 60-61 0.7978545763184499 1104 62-63 0.8576948942610301 1104 64-65 0.6769551206120283 1104 66-67 0.722946526969654 1104 68-69 0.6807111103470938 1104 70-71 1.0021580449675582 1104 72-73 1.0098052247294191 1104 74-75 0.6556110190701148 1104 76-77 0.41576601440133487 1104 78-79 0.7134926666436918 1104 80-81 0.8914579582247981 1104 82-83 0.9165406063448707 1104 84-85 0.47387150433489467 1104 86-87 0.41046653975518765 1104 88-89 0.397233986131436 1104 90-91 0.340928469001355 1104 92-93 0.14603222800063875 1104 94-95 -0.03958372991309034 1104 96-97 -0.1970990857184738 1104 98-99 0.057472618733168446 1104 100-101 0.22482683303676865 1105 1 -0.12107960091230652 1105 2 -0.11799055298995853 1105 3 0.05019686172106219 1105 4 0.15740774242367195 1105 5 0.07368655797027657 1105 6 -0.011120128551482367 1105 7 0.11982195487320269 1105 8 0.19667064555895308 1105 9 0.023693815346888414 1105 10-11 -0.006638734919540923 1105 12-13 0.05051396787718687 1105 14-15 0.017818742552243805 1105 16-17 -0.06199341464801478 1105 18-19 0.0750949931929199 1105 20-21 0.12186821537987669 1105 22-23 0.1413271705186574 1105 24-25 -0.16067491268501755 1105 26-27 -0.39091147249558844 1105 28-29 -0.11760340290926052 1105 30-31 -0.3194838976162657 1105 32-33 0.12792250068303446 1105 34-35 0.17255518969293604 1105 36-37 -0.037973125041212086 1105 38-39 -0.08463096141917958 1105 40-41 0.0021449811465572566 1105 42-43 -0.3226424200434863 1105 44-45 -0.04693649998447569 1105 46-47 0.011281404808968887 1105 48-49 0.05691263816445513 1105 50-51 0.007519821879050426 1105 52-53 -0.2218209063926082 1105 54-55 -0.14107215193477884 1105 56-57 -0.17860572820649168 1105 58-59 -0.09636975662999703 1105 60-61 -0.06983773137385896 1105 62-63 -0.3330743365082043 1105 64-65 -0.30304487938796854 1105 66-67 -0.01782270379957751 1105 68-69 -0.286211966575987 1105 70-71 -0.4132265704170557 1105 72-73 -0.26250246757827256 1105 74-75 -0.2982351347760357 1105 76-77 -0.02654167790635853 1105 78-79 0.11426189741291992 1105 80-81 0.10453488130172417 1105 82-83 0.051155990960253916 1105 84-85 -0.09074388028049185 1105 86-87 0.25662038590903435 1105 88-89 0.07261860151605148 1105 90-91 -0.019071530998644448 1105 92-93 -0.03319854123013144 1105 94-95 -0.018814499143857688 1105 96-97 0.015208606589219187 1105 98-99 0.23516492642547604 1105 100-101 0.3579037561136893 1106 1 0.05962688293617191 1106 2 0.24477031152317252 1106 3 0.2846240055741873 1106 4 0.2514723485124435 1106 5 0.28283366464004445 1106 6 0.20406927419079324 1106 7 0.1813836560119384 1106 8 0.3539609081664352 1106 9 0.43621066405709286 1106 10-11 0.39533895506419725 1106 12-13 0.43152256652928855 1106 14-15 0.5187692422036463 1106 16-17 0.5025754907668087 1106 18-19 0.3193710796442275 1106 20-21 0.44646965623509516 1106 22-23 0.3286267289662561 1106 24-25 0.18145383470052678 1106 26-27 0.035735053184161814 1106 28-29 0.08120440559643072 1106 30-31 0.14686516117991744 1106 32-33 0.055410299892876935 1106 34-35 0.08993841070153508 1106 36-37 0.28655162513308596 1106 38-39 0.3119109860593312 1106 40-41 0.12808967089789292 1106 42-43 0.37869618093257174 1106 44-45 0.5078810691533349 1106 46-47 0.28904575526213705 1106 48-49 0.18017547804360134 1106 50-51 -0.05623569752602009 1106 52-53 0.11305243778586771 1106 54-55 -0.05809748309908258 1106 56-57 0.06575402080582649 1106 58-59 0.07987937188499217 1106 60-61 0.3464811159419696 1106 62-63 0.017441582617983897 1106 64-65 0.08843083522974382 1106 66-67 -0.04457380768059238 1106 68-69 0.23535205852278196 1106 70-71 0.16421242563221128 1106 72-73 -0.08631840994406303 1106 74-75 0.018081388579762603 1106 76-77 -0.42510082268906757 1106 78-79 -0.058945167425566325 1106 80-81 -0.3901990252750842 1106 82-83 -0.4717907903551044 1106 84-85 -0.39455052680617086 1106 86-87 -0.4083157051901978 1106 88-89 -0.5042765878866895 1106 90-91 -0.3802218912124502 1106 92-93 -0.31939421866448114 1106 94-95 -0.2542943968896161 1106 96-97 0.19758136977769425 1106 98-99 0.17552514022979793 1106 100-101 0.18452936615319615 1107 1 -0.10372631589944703 1107 2 -0.02209623454244891 1107 3 -0.2054355909326695 1107 4 -0.2171912755796015 1107 5 -0.2596919783869822 1107 6 -0.07702146126132448 1107 7 -0.42095429015836316 1107 8 -0.18820660485021534 1107 9 -0.14440531956075375 1107 10-11 -0.32441053758495997 1107 12-13 8.459762942933935E-4 1107 14-15 0.024788581224207462 1107 16-17 -0.09973949835154627 1107 18-19 -0.04120615246993253 1107 20-21 0.07337394369411498 1107 22-23 -0.04307778622672487 1107 24-25 -0.12902422294921934 1107 26-27 -0.17516679164452142 1107 28-29 -0.24059147866329766 1107 30-31 -0.18754562312480516 1107 32-33 -0.22441091058655616 1107 34-35 -0.17085140371366236 1107 36-37 0.023663536169927113 1107 38-39 0.0964843062918348 1107 40-41 -0.012418965545045069 1107 42-43 0.05682683410661582 1107 44-45 0.1841062870157586 1107 46-47 0.12894097927704706 1107 48-49 0.17378193907630646 1107 50-51 0.4429745798870002 1107 52-53 0.5202623295204134 1107 54-55 0.40115604539980154 1107 56-57 0.3449668694086654 1107 58-59 0.6502633974499687 1107 60-61 0.5840154367346315 1107 62-63 0.16088872171017243 1107 64-65 0.1363238370020241 1107 66-67 0.017426302513257497 1107 68-69 0.027643539619948143 1107 70-71 -0.07835867235766969 1107 72-73 0.11073344544487185 1107 74-75 0.1322628778496373 1107 76-77 -0.05652764938169241 1107 78-79 -0.20910494850711459 1107 80-81 -0.24882261226853686 1107 82-83 -0.16129666276899357 1107 84-85 0.030715086284857307 1107 86-87 -0.13693350700656026 1107 88-89 -0.1433435214673473 1107 90-91 9.610987112296243E-5 1107 92-93 0.3781879446244396 1107 94-95 0.424561699125956 1107 96-97 0.4926460627500795 1107 98-99 0.5642109867481331 1107 100-101 0.5351845604157717 1108 1 0.14341605027938087 1108 2 -0.031527207231455634 1108 3 0.014699290276354304 1108 4 0.16969284351934277 1108 5 0.22323586329051182 1108 6 0.05179187867770452 1108 7 0.14042514023409325 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-1.4971332059120215 2316 62-63 -1.7862519340060068 2316 64-65 -1.9332335217494183 2316 66-67 -1.962302226817858 2316 68-69 -1.7400209784027858 2316 70-71 -1.578489985527991 2316 72-73 -1.6425493838132468 2316 74-75 -1.6178549177884491 2316 76-77 -1.714377666568268 2316 78-79 -2.0296751566384934 2316 80-81 -1.7960789394391625 2316 82-83 -1.5474394542552652 2316 84-85 -1.6865126223389382 2316 86-87 -1.905039275903107 2316 88-89 -1.8207456835000748 2316 90-91 -1.9179142638693314 2316 92-93 -2.237161985663718 2316 94-95 -2.240766410003012 2316 96-97 -2.016068883392208 2316 98-99 -2.3932203731542323 2316 100-101 -3.1920239146907257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4580.0 3 919.0 4 856.0 5 827.0 6 895.0 7 1040.0 8 1405.0 9 1725.0 10 2122.0 11 3088.0 12 4139.0 13 5542.0 14 6082.0 15 6594.0 16 7959.0 17 10064.0 18 8418.0 19 5609.0 20 5343.0 21 5545.0 22 5990.0 23 6565.0 24 7362.0 25 8207.0 26 9586.0 27 11147.0 28 12904.0 29 14942.0 30 17839.0 31 21011.0 32 25327.0 33 31454.0 34 41128.0 35 57707.0 36 80962.0 37 115813.0 38 105050.0 39 18434.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63154259230375 19.871060703966965 14.79296181584557 21.704434887883718 2 16.009638296640404 21.967061729934688 39.21414923325658 22.80915074016833 3 18.574414641243223 26.581047618482074 31.727840095961422 23.116697644313273 4 12.779373881799216 17.990393680912824 37.631975531354165 31.5982569059338 5 12.772264141696898 37.80771955252673 34.44855141442957 14.9714648913468 6 29.111818431381277 36.87418849837885 18.457200940785423 15.556792129454447 7 25.361561816460448 34.831320616962614 22.462537951748228 17.34457961482871 8 25.04726599252648 33.75682277553032 21.566797161369212 19.629114070573987 9 26.16583463431139 16.815265073042745 22.23811756884301 34.78078272380286 10-11 23.779310569984016 27.114716536496395 28.208191303547764 20.89778158997182 12-13 25.207674630341014 25.599983972230405 28.554658775463565 20.63768262196502 14-15 22.500771687383505 26.65986988163503 27.987691586233097 22.85166684474837 16-17 20.321686893600095 29.423881137457077 28.870422969960853 21.384008998981976 18-19 20.162831837393743 27.412499406373176 32.24135679346535 20.183311962767725 20-21 21.371200529525343 26.69650688397785 32.1122688706477 19.820023715849103 22-23 21.078051933690997 26.67143617309449 31.988075244406282 20.26243664880823 24-25 20.69256280302963 27.050432443506224 32.14236936880766 20.114635384656495 26-27 20.30461788782033 27.107711127408336 32.21698995543336 20.370681029337973 28-29 20.223107924561912 27.420231123778137 32.17563625837768 20.18102469328227 30-31 20.42027489445261 27.23527067552555 31.884327929000317 20.460126501021524 32-33 20.08785729126473 27.496364014128403 31.701418776527856 20.714359918079012 34-35 20.79460957902026 27.35018323355342 31.515799291759734 20.339407895666582 36-37 20.856720959236615 27.07210516199113 31.42455041371326 20.646623465059 38-39 20.474218679585817 27.317257391603594 31.473672684530314 20.73485124428027 40-41 20.592421032249895 27.240569111442923 31.51382005443869 20.65318980186849 42-43 20.3833180874172 27.476095694692336 30.98859088999401 21.15199532789645 44-45 20.49548358258991 27.457657422755293 30.970145084089175 21.076713910565623 46-47 20.795724430167382 27.663067932928264 30.333958120394183 21.20724951651017 48-49 20.65544822242174 27.803611410178558 29.973214318976854 21.56772604842285 50-51 20.565904900262613 28.092901976122626 29.781825631856364 21.559367491758398 52-53 20.741827425993094 28.138494917193924 29.60395537978739 21.5157222770256 54-55 20.571757648541812 28.022054978015053 29.647197831752198 21.75898954169094 56-57 20.437816474317817 28.264331566018086 29.330828602202118 21.96702335746198 58-59 20.56989316178847 27.77793499744559 29.543975251307366 22.10819658945857 60-61 20.42625772607949 27.743828442693374 29.818013695876495 22.01190013535064 62-63 20.423884924025334 27.60875896749838 29.92550393411631 22.041852174359974 64-65 20.396413958170793 27.698007646904095 29.672551250725604 22.233027144199507 66-67 20.193122381137258 27.761603103550293 29.45630490622953 22.588969609082923 68-69 20.387141200564006 27.48517702610453 29.59445118479456 22.533230588536902 70-71 20.402718573941037 27.260485289295616 29.44160431633231 22.895191820431037 72-73 20.22795844110167 27.297114230338472 29.38213813870204 23.092789189857815 74-75 20.08407731775176 27.568702136505678 29.16985197802247 23.17736856772009 76-77 20.116857642419784 27.15886038841794 29.536093188121733 23.188188781040544 78-79 20.05846755423145 27.217577506749834 29.518219905036773 23.205735033981938 80-81 20.156865081877466 27.077773350067595 29.319916130065437 23.445445437989505 82-83 20.083610321077337 26.8841001834004 29.475021938025264 23.557267557496996 84-85 20.009177291566722 26.66018098334083 29.50640771607605 23.82423400901639 86-87 20.181256728215484 26.45342491777143 29.502978108388987 23.8623402456241 88-89 20.188530794952644 26.259827383755237 29.471794887078218 24.0798469342139 90-91 20.133324094377425 26.137229788480067 29.608841978835343 24.12060413830717 92-93 20.324982750938453 25.967251371356276 29.45275396355866 24.255011914146614 94-95 20.219244551112727 25.596413879229218 29.57387246351326 24.610469106144794 96-97 20.23014354352206 25.172499612527865 29.770825136807467 24.826531707142603 98-99 20.33465514440823 25.206877590161074 29.600744505989873 24.857722759440815 100-101 20.609812337353205 24.718289974512924 29.961057896113164 24.710839792020707 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2156.0 1 1364.0 2 692.0 3 780.0 4 646.5 5 644.5 6 1001.0 7 1632.5 8 2273.5 9 2512.0 10 2087.5 11 1693.5 12 1752.5 13 1841.0 14 1861.0 15 1878.0 16 2007.5 17 2155.0 18 2218.5 19 2337.5 20 2550.0 21 2862.0 22 3170.5 23 3546.0 24 4043.5 25 4682.0 26 5552.5 27 6633.0 28 7519.5 29 8329.0 30 9353.0 31 10577.5 32 12115.5 33 13239.5 34 14098.5 35 15315.5 36 16964.5 37 18165.5 38 18798.5 39 19262.0 40 19371.5 41 19825.0 42 20161.0 43 20672.0 44 21643.0 45 22192.5 46 22509.0 47 23140.5 48 24547.0 49 24551.5 50 22790.0 51 21739.5 52 21058.0 53 20584.5 54 19743.5 55 18112.0 56 16607.5 57 14681.0 58 13145.5 59 11994.0 60 10174.0 61 8342.5 62 6839.5 63 5142.0 64 3584.0 65 2704.0 66 2048.5 67 1563.0 68 1240.0 69 995.5 70 729.5 71 523.5 72 450.5 73 379.5 74 297.0 75 231.5 76 168.5 77 110.0 78 82.0 79 71.0 80 55.0 81 36.5 82 26.5 83 25.0 84 20.0 85 12.0 86 5.5 87 4.0 88 4.0 89 4.0 90 3.5 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14788291588918126 2 0.091814969844938 3 0.08573352596183227 4 0.09893470902515937 5 0.11881064756994403 6 0.04108682818586079 7 0.04449836987638353 8 0.04983817426154956 9 0.048948206864021876 10-11 0.04761325576773037 12-13 0.0521372567051627 14-15 0.04954151846237366 16-17 0.04865155106484599 18-19 0.05310138805248435 20-21 0.05502965074712763 22-23 0.06786001406148488 24-25 0.08425024696595282 26-27 0.08662349335935994 28-29 0.07668552408696762 30-31 0.06415181657178624 32-33 0.05369469965083613 34-35 0.28961022394546276 36-37 0.4546250122370517 38-39 0.4346749097424731 40-41 0.41301903640263316 42-43 0.4396438943786693 44-45 0.4478760928058002 46-47 0.4479502567555942 48-49 0.461967243266655 50-51 0.45158429029549885 52-53 0.459223177124278 54-55 0.45106514264694103 56-57 0.4329691388972118 58-59 0.4142798235491307 60-61 0.3846142436315416 62-63 0.43586153293917673 64-65 0.47338849153492674 66-67 0.47591006582792184 68-69 0.4841422642550528 70-71 0.480879050464118 72-73 0.4720535404386353 74-75 0.46471130940903205 76-77 0.44846940440415195 78-79 0.4256269078676085 80-81 0.43081838435318653 82-83 0.44097884547496075 84-85 0.4390505827803175 86-87 0.4501010112996194 88-89 0.4595198329234539 90-91 0.46137393166830326 92-93 0.46441465360985607 94-95 0.467307047651821 96-97 0.46960613009543417 98-99 0.46471130940903205 100-101 0.45351255299014215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 674182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.39704420520528 #Duplication Level Percentage of deduplicated Percentage of total 1 85.01344401504373 56.44641400303604 2 9.014810179122373 11.971134999294454 3 2.424349065801201 4.829088362725514 4 1.0700994093201597 2.842057511383788 5 0.6057380405916468 2.0109607728969006 6 0.3726081042312968 1.4844046060717895 7 0.265014898050543 1.2317344130629915 8 0.19004446274740608 1.0094712475195209 9 0.15950895698048811 0.953183094098369 >10 0.8136755952355607 9.855563446337365 >50 0.04833165573226968 2.1839536999368527 >100 0.02013805542919045 2.1618054604818404 >500 0.0011187808570666629 0.5522253381959539 >1k 8.950246856533302E-4 1.654298934843139 >5k 2.2375617141333256E-4 0.81370411011548 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5477 0.8123919060431753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4335 0.643001444713742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3011 0.4466153056593027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2480 0.3678531909781038 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.19416122056062013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 985 0.14610298109412592 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.12355714035675826 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 744 0.11035595729343116 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.483278995879451E-4 7.416394979397254E-5 16-17 0.0 0.0 0.0 1.483278995879451E-4 2.2249184938191762E-4 18-19 0.0 0.0 0.0 3.7081974896986273E-4 2.966557991758902E-4 20-21 0.0 0.0 0.0 7.416394979397255E-4 2.966557991758902E-4 22-23 0.0 0.0 0.0 0.0026699021925830116 2.966557991758902E-4 24-25 0.0 0.0 0.0 0.006378099682281639 2.966557991758902E-4 26-27 0.0 0.0 0.0 0.012533707515181361 2.966557991758902E-4 28-29 0.0 0.0 0.0 0.03292879370852381 2.966557991758902E-4 30-31 0.0 0.0 0.0 0.08832926420462131 2.966557991758902E-4 32-33 0.0 0.0 0.0 0.1862256779326651 2.966557991758902E-4 34-35 0.0 0.0 0.0 0.3518337778226058 2.966557991758902E-4 36-37 0.0 0.0 0.0 0.5855985475732073 2.966557991758902E-4 38-39 0.0 0.0 0.0 0.8988670715029472 2.966557991758902E-4 40-41 0.0 0.0 0.0 1.2343847803708792 2.966557991758902E-4 42-43 0.0 0.0 0.0 1.558777896769715 2.966557991758902E-4 44-45 0.0 0.0 0.0 1.9717969331723482 2.966557991758902E-4 46-47 0.0 0.0 0.0 2.4377690297278773 2.966557991758902E-4 48-49 0.0 0.0 0.0 2.9059660447772266 2.966557991758902E-4 50-51 0.0 0.0 0.0 3.3948548018190934 2.966557991758902E-4 52-53 0.0 0.0 0.0 3.8755855243836232 3.7081974896986273E-4 54-55 0.0 0.0 0.0 4.389541696455853 4.449836987638353E-4 56-57 0.0 0.0 0.0 4.97306365343483 4.449836987638353E-4 58-59 0.0 7.416394979397254E-5 0.0 5.598933225746164 4.449836987638353E-4 60-61 0.0 1.483278995879451E-4 0.0 6.27338018517255 4.449836987638353E-4 62-63 0.0 1.483278995879451E-4 0.0 6.927283730506006 4.449836987638353E-4 64-65 0.0 1.483278995879451E-4 0.0 7.602546493380126 4.449836987638353E-4 66-67 0.0 1.483278995879451E-4 0.0 8.355533075638329 5.191476485578078E-4 68-69 0.0 1.483278995879451E-4 0.0 9.185353509883088 5.933115983517804E-4 70-71 0.0 1.483278995879451E-4 0.0 10.052700902723597 5.933115983517804E-4 72-73 0.0 1.483278995879451E-4 0.0 10.939182594610951 5.933115983517804E-4 74-75 0.0 1.483278995879451E-4 0.0 11.857183965160743 5.933115983517804E-4 76-77 0.0 1.483278995879451E-4 0.0 12.796767045100582 5.933115983517804E-4 78-79 0.0 1.483278995879451E-4 0.0 13.81941374880967 5.933115983517804E-4 80-81 0.0 1.483278995879451E-4 0.0 14.90784387598601 5.933115983517804E-4 82-83 0.0 1.483278995879451E-4 0.0 15.995680691564 7.416394979397255E-4 84-85 0.0 1.483278995879451E-4 0.0 17.08863481967777 7.416394979397255E-4 86-87 0.0 1.483278995879451E-4 0.0 18.202280689784065 7.416394979397255E-4 88-89 0.0 1.483278995879451E-4 0.0 19.36205653666221 7.416394979397255E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3125 0.0 40.646313 1 GTATCAA 4900 0.0 35.40148 1 GACTGCG 80 4.585788E-4 29.582575 7 TATCAAC 6105 0.0 28.088818 2 ATCAACG 6090 0.0 28.08022 3 TCAACGC 6145 0.0 27.828892 4 CAACGCA 6225 0.0 27.395153 5 AACGCAG 6265 0.0 27.198027 6 TAAGGCG 210 4.3655746E-11 27.06902 5 ACGCAGA 6995 0.0 24.15664 7 CGCAGAG 7055 0.0 23.749924 8 GAGTACT 4265 0.0 23.418098 12-13 GTACATG 3470 0.0 23.356245 1 TACATGG 3450 0.0 22.930206 2 GCAGAGT 7325 0.0 22.553085 9 ACATGGG 3455 0.0 22.34859 3 GTATAGG 170 3.2065236E-6 22.303728 1 TAAGACT 345 0.0 21.969059 4 GTACTTT 4635 0.0 21.498426 14-15 AGTACTT 4270 0.0 21.118122 12-13 >>END_MODULE