FastQCFastQC Report
Thu 26 May 2016
SRR938839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674182
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.7710084220581387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.6811217149078439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.4470602893580665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25630.3801644066439033No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT22550.33447941357081623No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT11980.17769682370635823TruSeq Adapter, Index 10 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.1759168889113029No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.15411268767187494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.12326048455758237No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7830.11614074537736102TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.040.14511
GTATCAA51150.032.8006481
CCGTCCA753.094312E-431.6365789
TATCAAC63650.026.2436962
ATCAACG63800.026.1819953
TCAACGC64400.025.9380664
CAACGCA64650.025.9111675
AACGCAG66450.025.1378696
GTACATG34000.024.1120931
TACATGG34200.023.3111652
ACGCAGA73900.022.4110287
GAGTACT44100.022.27638612-13
CGCAGAG74950.022.0970638
ACATGGG33850.021.7297043
CATGGGG13250.021.4889984
AGTACTT45150.021.02254112-13
GCAGAGT77300.020.7499949
TAGTGCC1150.003742257620.6325514
GTACTTT47400.020.57531514-15
ATAGGAC1456.1818707E-419.6364963
Produced by FastQC (version 0.11.5)