##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938839_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 674182 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906609787861438 31.0 31.0 34.0 30.0 34.0 2 32.11390692720957 33.0 31.0 34.0 30.0 34.0 3 32.18215852692597 34.0 31.0 34.0 30.0 34.0 4 35.86828482516591 37.0 35.0 37.0 35.0 37.0 5 35.77500882551003 37.0 35.0 37.0 35.0 37.0 6 35.80885725219599 37.0 35.0 37.0 33.0 37.0 7 35.735448291410925 37.0 35.0 37.0 33.0 37.0 8 35.70863357372342 37.0 35.0 37.0 33.0 37.0 9 37.32851663200738 39.0 37.0 39.0 34.0 39.0 10-11 37.26849574743912 39.0 37.0 39.0 33.0 39.0 12-13 37.19574462088872 39.0 37.0 39.0 33.0 39.0 14-15 38.36259200037972 40.0 38.0 41.0 33.0 41.0 16-17 38.294985627026534 40.0 38.0 41.0 33.0 41.0 18-19 38.33891441776849 40.0 38.0 41.0 33.5 41.0 20-21 38.39321874508664 40.0 38.0 41.0 34.0 41.0 22-23 38.32247523665717 40.0 38.0 41.0 33.5 41.0 24-25 38.22599609600968 40.0 37.5 41.0 33.0 41.0 26-27 38.01786090402888 40.0 37.0 41.0 33.0 41.0 28-29 37.80474634445891 40.0 37.0 41.0 33.0 41.0 30-31 37.50343898235195 39.5 36.5 41.0 32.0 41.0 32-33 37.16649435908998 39.0 35.5 41.0 31.5 41.0 34-35 37.02168405564076 39.0 35.0 41.0 31.0 41.0 36-37 36.85662031914231 39.0 35.0 41.0 31.0 41.0 38-39 36.67142552011178 39.0 35.0 41.0 30.5 41.0 40-41 36.44957741381407 39.0 35.0 41.0 30.0 41.0 42-43 36.18075831155385 39.0 35.0 41.0 30.0 41.0 44-45 35.811536795702054 39.0 35.0 41.0 28.5 41.0 46-47 35.33905384599411 39.0 35.0 41.0 26.5 41.0 48-49 34.89591757121964 39.0 35.0 41.0 24.0 41.0 50-51 34.565441527658706 38.5 34.0 40.0 23.5 41.0 52-53 34.33409301939239 38.0 34.0 40.0 23.0 41.0 54-55 34.05082158823582 38.0 34.0 40.0 21.5 41.0 56-57 33.73778964730592 38.0 33.5 40.0 20.0 41.0 58-59 33.387574868507315 37.0 33.0 40.0 18.0 41.0 60-61 32.99845664820479 37.0 33.0 40.0 14.0 41.0 62-63 32.5845936260535 36.0 32.0 39.5 9.5 41.0 64-65 32.18670996852482 36.0 32.0 39.0 7.0 41.0 66-67 31.745076255373178 35.0 31.0 39.0 2.0 40.0 68-69 31.266804067744317 35.0 31.0 38.5 2.0 40.0 70-71 30.799060936067708 35.0 30.5 37.5 2.0 40.0 72-73 30.467369345369647 35.0 30.0 37.0 2.0 39.5 74-75 30.051366693266804 35.0 30.0 36.5 2.0 39.0 76-77 28.048291855908346 32.5 27.0 34.5 2.0 36.5 78-79 29.048591478265514 34.0 29.0 35.0 2.0 37.5 80-81 29.08192149894242 34.0 29.0 35.0 2.0 37.0 82-83 28.7695815076641 34.0 29.0 35.0 2.0 37.0 84-85 28.391347588633337 34.0 29.0 35.0 2.0 36.0 86-87 27.87231415255821 34.0 27.5 35.0 2.0 36.0 88-89 27.364596651942648 33.5 26.5 35.0 2.0 35.5 90-91 26.960055296640967 33.0 25.5 35.0 2.0 35.0 92-93 26.673709769765434 33.0 25.0 35.0 2.0 35.0 94-95 26.29207024216013 33.0 24.5 35.0 2.0 35.0 96-97 25.803909033465743 33.0 23.5 35.0 2.0 35.0 98-99 25.10598325081358 33.0 18.0 35.0 2.0 35.0 100-101 22.354483655748744 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0941998717657917 1101 2 0.1373739505866709 1101 3 0.1945121103068317 1101 4 0.1574284320070234 1101 5 0.1160540690861609 1101 6 0.1280147762312538 1101 7 0.19091899825540537 1101 8 0.1582006637832265 1101 9 0.18234817698307637 1101 10-11 0.1266398852597348 1101 12-13 0.2031748673787135 1101 14-15 0.279627038749787 1101 16-17 0.2539314477138248 1101 18-19 0.07320628325711453 1101 20-21 0.11477221665285953 1101 22-23 -0.00825389390186615 1101 24-25 0.16849724100169539 1101 26-27 0.26213872703103647 1101 28-29 0.09402263117186038 1101 30-31 0.131878969537496 1101 32-33 0.10623741766518435 1101 34-35 -0.09492431579675298 1101 36-37 -0.18398629792274335 1101 38-39 -0.0126295568228727 1101 40-41 0.22377234097641008 1101 42-43 0.2988692712843317 1101 44-45 0.41392843743810204 1101 46-47 0.42226246774534104 1101 48-49 0.4172198923759751 1101 50-51 0.506628026861371 1101 52-53 0.5344020909604552 1101 54-55 0.5277313199643601 1101 56-57 0.2736717951098555 1101 58-59 0.12956970307528337 1101 60-61 0.2061030546203284 1101 62-63 0.305334341086386 1101 64-65 0.31029703336259473 1101 66-67 0.19145338197190753 1101 68-69 -0.13352615679586322 1101 70-71 -0.1996739948769921 1101 72-73 -0.20651060217697648 1101 74-75 -0.10849627097484316 1101 76-77 -0.06573167423435322 1101 78-79 0.01658482965965291 1101 80-81 0.14505254053601035 1101 82-83 -0.18001128918239218 1101 84-85 -0.2506507793316324 1101 86-87 -0.4348229816980975 1101 88-89 -0.3419177013268673 1101 90-91 -0.43872396789209134 1101 92-93 -0.3022286901054585 1101 94-95 -0.35376910602284184 1101 96-97 -0.40951961493074407 1101 98-99 -0.3372821269210817 1101 100-101 0.006446803637487619 1102 1 -0.024672677253818165 1102 2 -0.050628814621973106 1102 3 0.01357948084478977 1102 4 0.06696211727600598 1102 5 0.1256694537015477 1102 6 -0.045690603356476345 1102 7 0.11022518226747025 1102 8 0.13469639027895397 1102 9 0.09643817195542681 1102 10-11 0.11284526484747204 1102 12-13 0.2142357019689598 1102 14-15 0.11508141280709339 1102 16-17 0.3757891651597802 1102 18-19 0.3331358961279065 1102 20-21 0.3142914474220859 1102 22-23 0.20927061590205653 1102 24-25 0.18595262058943263 1102 26-27 0.3710973745926296 1102 28-29 0.25030405399740374 1102 30-31 0.0370448820563567 1102 32-33 0.26415282741883317 1102 34-35 -0.019006769290967895 1102 36-37 0.1809269750787621 1102 38-39 0.13164834664620173 1102 40-41 0.07550373866369142 1102 42-43 -0.021077938368755156 1102 44-45 0.20207574764422986 1102 46-47 0.11821520781572303 1102 48-49 -0.03627432582412382 1102 50-51 -0.16277242562731686 1102 52-53 -0.10604222779269179 1102 54-55 -0.15793044474151685 1102 56-57 -0.08827202087807251 1102 58-59 0.12689876290433943 1102 60-61 0.03879222003008209 1102 62-63 -0.10265962573111409 1102 64-65 0.24478044211070227 1102 66-67 -0.021122032809390845 1102 68-69 0.035434376135256684 1102 70-71 -0.0466762573204349 1102 72-73 -0.09546233671694537 1102 74-75 -0.2492705293961599 1102 76-77 -0.5369483660392795 1102 78-79 -0.41745865953089734 1102 80-81 -0.18975766559772822 1102 82-83 -0.20071893121356155 1102 84-85 0.017590170894614232 1102 86-87 -0.2816838414266023 1102 88-89 0.07842543592803608 1102 90-91 -0.07198817100923804 1102 92-93 -0.014112802724866924 1102 94-95 -0.0804533694718117 1102 96-97 -0.1005703942168168 1102 98-99 0.3114578177747447 1102 100-101 0.5761740535117958 1103 1 0.03149398941285142 1103 2 0.07883015973699514 1103 3 -0.06998462171931408 1103 4 0.0510467326606161 1103 5 -0.040612597580505394 1103 6 -0.20853162899750544 1103 7 0.008040566882854705 1103 8 0.11251177489434383 1103 9 -0.013105417788160878 1103 10-11 -0.039013709511507955 1103 12-13 0.06553826607152047 1103 14-15 0.085454489730175 1103 16-17 0.008266088236702274 1103 18-19 0.06161281920482509 1103 20-21 0.17790554998618546 1103 22-23 0.2155539492353853 1103 24-25 0.010843646230455306 1103 26-27 0.03198840023365079 1103 28-29 0.22852328476663075 1103 30-31 0.18570642051788866 1103 32-33 0.12657205818806005 1103 34-35 -0.11187856416276531 1103 36-37 -0.09042943517764712 1103 38-39 -0.15537857643072073 1103 40-41 0.13693835404830423 1103 42-43 0.2633477026568869 1103 44-45 -0.12207809850961127 1103 46-47 0.06613828473879835 1103 48-49 0.2301872126374107 1103 50-51 0.3332044974496071 1103 52-53 0.1942308491303848 1103 54-55 0.16445288859181062 1103 56-57 0.029726697070643127 1103 58-59 -0.1804525191469466 1103 60-61 0.00740632259418561 1103 62-63 -0.05875193342341589 1103 64-65 0.062367621597886114 1103 66-67 -0.03920151998888244 1103 68-69 -0.09557844437756557 1103 70-71 -0.027191641935818467 1103 72-73 0.1830658684112585 1103 74-75 0.14137562444999574 1103 76-77 0.19893368524277122 1103 78-79 0.17505672508449166 1103 80-81 0.26072182158176105 1103 82-83 0.3670579918633585 1103 84-85 0.49207863243307415 1103 86-87 0.762078979086219 1103 88-89 0.6799921025946993 1103 90-91 0.4176708033497363 1103 92-93 0.5495307870187247 1103 94-95 0.5791158612974172 1103 96-97 0.3893731955267725 1103 98-99 0.08106166392859393 1103 100-101 0.5584637971015418 1104 1 0.09641706633592761 1104 2 0.198153236660076 1104 3 0.07678460904991624 1104 4 0.01215442496830832 1104 5 0.18246432549641867 1104 6 0.11251452484864899 1104 7 -0.08746712542483692 1104 8 -0.024534378951813096 1104 9 -0.1990746485573922 1104 10-11 0.14637090587310553 1104 12-13 0.07987672760998521 1104 14-15 0.16825448973017387 1104 16-17 0.04309685746746794 1104 18-19 0.2527512807432899 1104 20-21 0.1938747807554151 1104 22-23 0.08173856461999662 1104 24-25 0.1651513385381449 1104 26-27 0.1172191694644269 1104 28-29 0.015784823228166545 1104 30-31 -0.29795511794365126 1104 32-33 -0.1299817879657823 1104 34-35 -0.05157087185506981 1104 36-37 -0.04759866594687878 1104 38-39 0.09212911587697192 1104 40-41 -0.13247703056708104 1104 42-43 0.22494770265689112 1104 44-45 0.23361420918269715 1104 46-47 0.06321520781573042 1104 48-49 0.08372567417587362 1104 50-51 0.04126603591114275 1104 52-53 0.2084770029765437 1104 54-55 0.19319135013027022 1104 56-57 0.1678497739937157 1104 58-59 0.03388594239151388 1104 60-61 0.3595293995172639 1104 62-63 0.2881096050381231 1104 64-65 0.37753685236711476 1104 66-67 0.26535232616496884 1104 68-69 0.3524215556224348 1104 70-71 0.1935160503718727 1104 72-73 0.41466586841125874 1104 74-75 0.45996023983461143 1104 76-77 0.3139490698581575 1104 78-79 0.37850287893064305 1104 80-81 0.5445372061971447 1104 82-83 0.5413964534018199 1104 84-85 0.5544170939715372 1104 86-87 0.534694363701604 1104 88-89 0.36220748721008533 1104 90-91 0.6494861879651204 1104 92-93 0.6346692485571879 1104 94-95 0.67262355360511 1104 96-97 0.9003270416806188 1104 98-99 0.7830924331593607 1104 100-101 0.9880330278707739 1105 1 -0.17435216443330503 1105 2 -0.1495390710322333 1105 3 -0.006292314027007251 1105 4 -0.17246095964707564 1105 5 -0.12368952065742889 1105 6 -0.0921008597667381 1105 7 0.0033021053443960113 1105 8 -0.08453437895181537 1105 9 -0.15907464855739306 1105 10-11 -0.023629094126896177 1105 12-13 -0.03704634931309414 1105 14-15 0.07440833588402285 1105 16-17 -0.12690314253253376 1105 18-19 -0.1926333346413287 1105 20-21 -0.1484329115522698 1105 22-23 0.006353949235389678 1105 24-25 -0.13484866146185226 1105 26-27 0.027219169464423487 1105 28-29 0.01732328476662559 1105 30-31 -0.2125705025590321 1105 32-33 0.0015566735726721959 1105 34-35 0.10996758968339293 1105 36-37 -0.01529097363918197 1105 38-39 -0.08556319181533212 1105 40-41 -0.05247703056708275 1105 42-43 -0.08505229734311115 1105 44-45 0.07053728610577537 1105 46-47 0.17552290012341842 1105 48-49 0.28680259725280166 1105 50-51 0.4243429589880634 1105 52-53 0.07309238759192027 1105 54-55 0.019345196284120902 1105 56-57 0.11861900476295517 1105 58-59 0.1369628654684405 1105 60-61 -0.08277829279042948 1105 62-63 -0.23496731803880522 1105 64-65 -0.21630930147903626 1105 66-67 -0.25003228921965004 1105 68-69 -0.32296305976218065 1105 70-71 -0.33417625732043277 1105 72-73 -0.496872593127204 1105 74-75 -0.39003976016539 1105 76-77 -0.324512468603384 1105 78-79 -0.5845740441462794 1105 80-81 -0.6193089476490066 1105 82-83 -0.44552662352125694 1105 84-85 -0.27327521372077257 1105 86-87 -0.3876133286060863 1105 88-89 -0.30394635894376165 1105 90-91 -0.15897535049641576 1105 92-93 -0.3491769052889673 1105 94-95 -0.3889149079333514 1105 96-97 -0.43505757370399323 1105 98-99 -0.2445998745329483 1105 100-101 -0.07812081828307527 1106 1 -0.054947098200443634 1106 2 -0.04511425113913248 1106 3 -0.008546555254056898 1106 4 0.02770171755487638 1106 5 0.04379827855242979 1106 6 0.04573785740733172 1106 7 0.08774086899766331 1106 8 -0.011654992477261317 1106 9 0.10601482390367778 1106 10-11 -0.018802229148967342 1106 12-13 0.023806544017141107 1106 14-15 0.1307318311199026 1106 16-17 -0.07839977279049037 1106 18-19 0.03680194307190021 1106 20-21 0.11917805753905952 1106 22-23 0.17789473473387574 1106 24-25 -0.01848565890549736 1106 26-27 0.011069971230632802 1106 28-29 -0.26826583910043666 1106 30-31 -0.06082288461806229 1106 32-33 0.04429894640558274 1106 34-35 0.07887068055022439 1106 36-37 0.33014941678609944 1106 38-39 0.27229876495901095 1106 40-41 0.3437395625075155 1106 42-43 0.5160957389106642 1106 44-45 0.37782522475322367 1106 46-47 0.5134917590590433 1106 48-49 0.44780654798484676 1106 50-51 0.4731066122532255 1106 52-53 0.6190115137829579 1106 54-55 0.23875258647701258 1106 56-57 0.33237684812804247 1106 58-59 0.38733237511286944 1106 60-61 0.5102196156455463 1106 62-63 0.5103429306249225 1106 64-65 0.3613899780933494 1106 66-67 0.27587521717124375 1106 68-69 0.24532185773723825 1106 70-71 0.4952566964327616 1106 72-73 0.5654815783810498 1106 74-75 0.640980458286851 1106 76-77 0.4406932018590872 1106 78-79 0.4847171480452168 1106 80-81 0.5056922143310025 1106 82-83 0.5510966625582228 1106 84-85 0.4588302940644944 1106 86-87 0.5353357766692994 1106 88-89 0.8272295648303505 1106 90-91 1.0071668758572905 1106 92-93 1.1256685513691806 1106 94-95 1.0861164655270237 1106 96-97 1.2486142831400677 1106 98-99 1.4090278270705845 1106 100-101 2.145481319760151 1107 1 -0.2244573783683066 1107 2 -0.19956011381923489 1107 3 -0.1395165366129305 1107 4 -0.02453016703542943 1107 5 -0.09696518116712838 1107 6 -0.28181795118595687 1107 7 -0.18221110484966374 1107 8 -0.226128954588944 1107 9 -0.23304705912555335 1107 10-11 -0.335487873645242 1107 12-13 -0.38579547253029745 1107 14-15 -0.23837165008745842 1107 16-17 -0.14832236161132784 1107 18-19 -0.1886959953848404 1107 20-21 -0.14953882691350628 1107 22-23 -0.08629042766430928 1107 24-25 -0.05122930209780918 1107 26-27 -0.15331859064780673 1107 28-29 -0.15750954198109213 1107 30-31 -0.3306485946796798 1107 32-33 -0.31729298740464884 1107 34-35 -0.5767964972934649 1107 36-37 -0.47230055979770214 1107 38-39 -0.2829749290143013 1107 40-41 -0.22011088607327878 1107 42-43 -0.22085776846773086 1107 44-45 -0.34924527176188747 1107 46-47 -0.7444513809146969 1107 48-49 -0.7394541247485975 1107 50-51 -0.8101742259546469 1107 52-53 -0.9566457466142921 1107 54-55 -0.7671313059370632 1107 56-57 -1.0655170719263154 1107 58-59 -0.9357376255299243 1107 60-61 -0.9337766561292185 1107 62-63 -0.979500869593636 1107 64-65 -0.7449952963352473 1107 66-67 -0.8025644379220118 1107 68-69 -0.7685510887544638 1107 70-71 -0.8011624626045126 1107 72-73 -0.8755165024094111 1107 74-75 -0.9236918527536524 1107 76-77 -0.897140478890968 1107 78-79 -0.8210271654930175 1107 80-81 -0.5686496069896698 1107 82-83 -0.5833031023615654 1107 84-85 -0.4742338295730022 1107 86-87 -0.5932948810961527 1107 88-89 -0.3003106329676122 1107 90-91 -0.12479485014570457 1107 92-93 -0.42227954732777917 1107 94-95 -0.2888938651463526 1107 96-97 -0.2771641904914617 1107 98-99 -0.19373712026593637 1107 100-101 0.4335555904146098 1108 1 -0.19803905023472623 1108 2 -0.22540713835404347 1108 3 -0.31824306518480583 1108 4 -0.14447044795612385 1108 5 -0.27050980756581566 1108 6 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Count 3 8.0 4 6.0 5 34.0 6 110.0 7 238.0 8 527.0 9 923.0 10 1502.0 11 2298.0 12 3158.0 13 4336.0 14 4934.0 15 6359.0 16 7661.0 17 10098.0 18 9755.0 19 6241.0 20 5599.0 21 5683.0 22 5804.0 23 6574.0 24 6990.0 25 7877.0 26 8508.0 27 10220.0 28 12273.0 29 14832.0 30 17498.0 31 21156.0 32 25810.0 33 32285.0 34 41846.0 35 58659.0 36 83838.0 37 129044.0 38 109680.0 39 11817.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.0705488589261 20.911251131545093 15.271541045309448 21.746658964219353 2 16.12279770151087 22.583367695963403 38.50562607723137 22.78820852529436 3 18.325170354592675 26.795583388461868 32.233284187355935 22.64596206958952 4 12.908383789540508 18.852624365527408 36.786802376806264 31.45218946812582 5 12.507898460653058 38.420337534968304 34.474073766431026 14.597690237947617 6 29.20709837996268 37.41437771996286 17.787778374385553 15.59074552568891 7 25.083731099317397 35.270891243017466 22.010228691955586 17.635148965709558 8 25.250155002655067 34.43105867555052 21.083327647430515 19.235458674363894 9 25.753728221756145 16.77395718070195 21.76296608334248 35.70934851419943 10-11 23.704429961049094 27.416409812187215 27.96396225351611 20.91519797324758 12-13 25.138834914014318 25.746385990726537 28.271149333562747 20.8436297616964 14-15 22.366471318533154 26.882395083812803 27.554692285899485 23.19644131175456 16-17 20.37324635178873 29.847470800960004 28.45367461426414 21.32560823298713 18-19 20.16296798414077 27.46085438416806 31.87138774944136 20.50478988224981 20-21 21.250953934511703 26.71476449591097 31.90075669534094 20.13352487423639 22-23 21.00894120578716 26.9169897742746 31.722072081426084 20.35199693851215 24-25 20.77384148494027 27.24753849850634 31.91934818787805 20.059271828675342 26-27 20.442121298196405 27.27789178433404 32.04233578049828 20.237651136971273 28-29 20.16970194991857 27.454233426582142 32.00256013954689 20.373504483952402 30-31 20.637528145218948 27.39586639809428 31.66311174133988 20.303493715346896 32-33 20.51575093965724 27.339353468351273 31.675942104654233 20.468953487337245 34-35 20.59766142161535 27.422557305554218 31.733537053450572 20.246244219379868 36-37 20.97265790447735 27.084036462061462 31.38640542791889 20.5569002055423 38-39 20.364063051650017 27.23508190009405 31.5345772376779 20.866277810578033 40-41 20.465697909002444 27.217012199463603 31.70933413239029 20.607955759143664 42-43 20.534307777655815 27.28224076705599 31.22046333077213 20.962988124516066 44-45 20.390702641766914 27.572793212394497 30.978091579275258 21.05841256656333 46-47 20.8751314573559 27.568962269328495 30.202930108443976 21.352976164871627 48-49 20.697600944552065 27.72841754897046 29.947922074454674 21.626059432022807 50-51 20.519656973592188 28.083562811167123 29.798249862239135 21.598530353001554 52-53 20.62061876466592 28.18104013456307 29.77148603789481 21.426855062876196 54-55 20.460202141261558 28.18645410289803 29.402149567920826 21.951194187919583 56-57 20.497358280108337 28.50194754532159 28.957685016805552 22.04300915776452 58-59 20.191596000468717 28.246133445271955 29.36415996345184 22.198110590807484 60-61 20.32460390506626 28.533149009419656 29.17192926767585 21.970317817838232 62-63 20.069209797947735 28.61586337220513 29.187964080915833 22.126962748931298 64-65 19.897297762325305 28.612600158414196 29.03607631173778 22.45402576752272 66-67 20.01429894738091 28.54775944839289 28.71092171103815 22.72701989318805 68-69 20.24581100053176 28.19150474690233 28.728307794768877 22.83437645779703 70-71 20.27869804448578 28.00285684407728 28.529281539313867 23.189163572123075 72-73 20.24794491695121 27.92302375322984 28.356734531624994 23.47229679819396 74-75 20.19959002168554 28.108359463764977 28.07772975250007 23.614320762049417 76-77 20.183450823420415 27.699723249971793 28.48417001716325 23.632655909444537 78-79 20.111459132393332 27.71781788183136 28.29573763778453 23.874985347990783 80-81 20.256325443277927 27.63244939793705 28.116814154041492 23.994411004743526 82-83 20.24816740880059 27.396533873642433 28.123192253723772 24.232106463833208 84-85 20.09613131172295 27.183015862185584 28.107914480066214 24.612938346025256 86-87 20.26607584914545 27.12058901516136 27.928457207198843 24.684877928494345 88-89 20.267748593401 26.847551602577933 27.925286937267398 24.95941286675367 90-91 20.091744961501476 26.669329174466398 28.020217210063684 25.218708653968434 92-93 20.292591614727183 26.52770320180604 27.816672649225282 25.363032534241498 94-95 20.10089271212574 26.14481411904658 28.10422712578141 25.650066043046273 96-97 20.15316338911451 25.91703723920247 28.073428243412017 25.856371128271 98-99 20.114316396993985 25.90748930369641 27.84902878527518 26.129165514034426 100-101 20.18779934646243 25.536320580500373 28.25934435615573 26.01653571688147 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1722.0 1 1145.5 2 623.0 3 702.0 4 626.0 5 598.0 6 923.5 7 1490.5 8 2024.5 9 2246.5 10 1884.5 11 1524.5 12 1548.5 13 1577.0 14 1649.0 15 1721.5 16 1726.5 17 1816.0 18 2000.5 19 2167.0 20 2303.0 21 2507.5 22 2819.5 23 3275.0 24 3721.5 25 4301.5 26 5380.5 27 6468.5 28 7300.5 29 8161.0 30 9215.5 31 10515.5 32 12136.0 33 13291.0 34 14016.0 35 15105.0 36 16600.5 37 17765.0 38 18488.0 39 18986.0 40 19156.0 41 19586.0 42 19946.0 43 20673.5 44 21728.5 45 22599.0 46 22999.5 47 23571.5 48 24930.5 49 24629.0 50 23183.5 51 22656.5 52 22118.0 53 21695.5 54 20699.0 55 18904.0 56 17375.0 57 15607.0 58 13974.5 59 12532.5 60 10599.5 61 8530.0 62 6772.0 63 4962.0 64 3451.5 65 2543.5 66 1897.5 67 1458.0 68 1078.0 69 784.0 70 576.0 71 408.5 72 326.5 73 292.5 74 236.5 75 169.5 76 116.0 77 75.5 78 49.5 79 29.0 80 16.5 81 11.0 82 8.0 83 7.0 84 4.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3758628975558529 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.483278995879451E-4 16-17 0.0023732463934071214 18-19 7.416394979397254E-5 20-21 7.416394979397254E-5 22-23 0.0 24-25 0.0 26-27 7.416394979397254E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008083870527543007 36-37 0.016538560804055878 38-39 0.010086297171980268 40-41 0.008009706577749034 42-43 0.002966557991758902 44-45 0.001779934795055341 46-47 0.0013349510962915058 48-49 0.0 50-51 9.64131347321643E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.4498369876383524E-4 60-61 9.64131347321643E-4 62-63 0.0 64-65 0.0 66-67 9.64131347321643E-4 68-69 8.158034477336979E-4 70-71 0.0017057708452613684 72-73 0.0 74-75 0.0 76-77 0.09641313473216431 78-79 0.031593842612232304 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1469187845418596 88-89 0.3220198700054288 90-91 0.27937559887389457 92-93 0.0 94-95 7.416394979397254E-5 96-97 0.0 98-99 7.416394979397254E-5 100-101 7.416394979397255E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 674182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.4630350531549 #Duplication Level Percentage of deduplicated Percentage of total 1 84.16553096243864 54.25565572699076 2 9.50467441906322 12.254003204897938 3 2.5695280108016503 4.969187226911104 4 1.1288293984697237 2.9107107633034217 5 0.6174005030947918 1.9899755136417514 6 0.41544565435785596 1.6068532667730804 7 0.2749985656809525 1.240906952534099 8 0.21277733106270544 1.0973018040649538 9 0.15383336369865314 0.8924908964805902 >10 0.8750834315559931 10.188163003605306 >50 0.056132675065736784 2.4581928274598157 >100 0.022775024877854946 2.5005958122961114 >500 0.0011502537816394584 0.5150379110292629 >1k 0.001610355294295242 2.3500736470143186 >5k 2.300507563278917E-4 0.7708514429974398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5198 0.7710084220581387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4592 0.6811217149078439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3014 0.4470602893580665 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2563 0.3801644066439033 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2255 0.33447941357081623 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1198 0.17769682370635823 TruSeq Adapter, Index 10 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.1759168889113029 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.15411268767187494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 831 0.12326048455758237 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 783 0.11614074537736102 TruSeq Adapter, Index 10 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 2.966557991758902E-4 12-13 0.0 0.0 0.0 0.0 5.933115983517804E-4 14-15 0.0 0.0 0.0 1.483278995879451E-4 5.933115983517804E-4 16-17 0.0 0.0 0.0 1.483278995879451E-4 5.933115983517804E-4 18-19 0.0 0.0 0.0 3.7081974896986273E-4 7.416394979397255E-4 20-21 0.0 0.0 0.0 7.416394979397255E-4 7.416394979397255E-4 22-23 0.0 0.0 0.0 0.0026699021925830116 7.416394979397255E-4 24-25 0.0 0.0 0.0 0.006378099682281639 7.416394979397255E-4 26-27 0.0 0.0 0.0 0.012607871464975332 8.899673975276706E-4 28-29 0.0 0.0 0.0 0.03374459715625751 8.899673975276706E-4 30-31 0.0 1.483278995879451E-4 0.0 0.08929339555194295 8.899673975276706E-4 32-33 0.0 2.2249184938191762E-4 0.0 0.18763479297875055 8.899673975276706E-4 34-35 0.0 2.966557991758902E-4 0.0 0.3549486637139526 8.899673975276706E-4 36-37 0.0 2.966557991758902E-4 0.0 0.5932374344019864 8.899673975276706E-4 38-39 0.0 2.966557991758902E-4 0.0 0.9117715987670985 8.899673975276706E-4 40-41 0.0 2.966557991758902E-4 0.0 1.251071669074523 8.899673975276706E-4 42-43 0.0 3.7081974896986273E-4 0.0 1.5791729829630574 8.899673975276706E-4 44-45 0.0 4.449836987638353E-4 0.0 1.9967901842529168 8.899673975276706E-4 46-47 0.0 4.449836987638353E-4 0.0 2.4675829375450546 8.899673975276706E-4 48-49 0.0 4.449836987638353E-4 0.0 2.9400814616824538 8.899673975276706E-4 50-51 0.0 4.449836987638353E-4 0.0 3.433716711511135 8.899673975276706E-4 52-53 0.0 4.449836987638353E-4 0.0 3.9190455989628914 9.64131347321643E-4 54-55 0.0 4.449836987638353E-4 0.0 4.437599935922347 0.0010382952971156156 56-57 0.0 4.449836987638353E-4 0.0 5.0253492380395794 0.0010382952971156156 58-59 0.0 4.449836987638353E-4 0.0 5.655668647338553 0.0010382952971156156 60-61 0.0 4.449836987638353E-4 0.0 6.337902821493306 0.0010382952971156156 62-63 0.0 4.449836987638353E-4 0.0 6.999371089705747 0.0012607871464975333 64-65 0.0 4.449836987638353E-4 0.0 7.683607690504938 0.0013349510962915058 66-67 0.0 4.449836987638353E-4 0.0 8.445123126989447 0.0013349510962915058 68-69 0.0 4.449836987638353E-4 0.0 9.281544152765871 0.0013349510962915058 70-71 0.0 4.449836987638353E-4 0.0 10.156901252184127 0.0013349510962915058 72-73 0.0 4.449836987638353E-4 0.0 11.050651011151292 0.0013349510962915058 74-75 0.0 4.449836987638353E-4 0.0 11.980518613668119 0.0013349510962915058 76-77 0.0 4.449836987638353E-4 0.0 12.928259728085294 0.0013349510962915058 78-79 0.0 4.449836987638353E-4 0.0 13.961066892916158 0.0013349510962915058 80-81 0.0 4.449836987638353E-4 0.0 15.060473284662006 0.0013349510962915058 82-83 0.0 4.449836987638353E-4 0.0 16.16114046355435 0.0013349510962915058 84-85 0.0 4.449836987638353E-4 0.0 17.2568386578105 0.0013349510962915058 86-87 0.0 4.449836987638353E-4 0.0 18.375008528854227 0.0013349510962915058 88-89 0.0 4.449836987638353E-4 0.0 19.543461557858265 0.0013349510962915058 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3360 0.0 40.1451 1 GTATCAA 5115 0.0 32.800648 1 CCGTCCA 75 3.094312E-4 31.636578 9 TATCAAC 6365 0.0 26.243696 2 ATCAACG 6380 0.0 26.181995 3 TCAACGC 6440 0.0 25.938066 4 CAACGCA 6465 0.0 25.911167 5 AACGCAG 6645 0.0 25.137869 6 GTACATG 3400 0.0 24.112093 1 TACATGG 3420 0.0 23.311165 2 ACGCAGA 7390 0.0 22.411028 7 GAGTACT 4410 0.0 22.276386 12-13 CGCAGAG 7495 0.0 22.097063 8 ACATGGG 3385 0.0 21.729704 3 CATGGGG 1325 0.0 21.488998 4 AGTACTT 4515 0.0 21.022541 12-13 GCAGAGT 7730 0.0 20.749994 9 TAGTGCC 115 0.0037422576 20.632551 4 GTACTTT 4740 0.0 20.575315 14-15 ATAGGAC 145 6.1818707E-4 19.636496 3 >>END_MODULE