FastQCFastQC Report
Thu 26 May 2016
SRR938838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673297
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.8046968871092549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.6586989099906876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.4207652789185159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24690.36670295575355305No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.1834257393096954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.1476317286427832No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.1225313643161933No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.11495669815846499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34300.040.030511
GTATCAA50900.031.7357311
CTACCGT650.005881021729.1913419
ATAGCGT700.008444536527.1062456
AATAGCG700.008444536527.1062455
TATCAAC62750.025.7779332
ATCAACG64350.025.2107033
TCAACGC64750.025.1282234
CAACGCA65200.024.8820385
AACGCAG66700.024.3224726
GTACATG34700.023.275921
GTCTTAG2052.6589987E-823.1757351
TACATGG34300.022.6807382
GTCTAGC1050.002192519122.6239341
GTATAGG1503.0257293E-522.1714551
GAGTACT43050.022.00217412-13
ACGCAGA73550.021.9298697
CGCAGAG73900.021.6976178
ACATGGG34700.021.1889613
GCAGAGT76200.020.3563659
Produced by FastQC (version 0.11.5)