##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938838_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673297 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.509951774625463 33.0 31.0 34.0 30.0 34.0 2 31.551679273782597 33.0 31.0 34.0 30.0 34.0 3 31.54255402890552 33.0 31.0 34.0 28.0 34.0 4 35.12941985483375 37.0 35.0 37.0 33.0 37.0 5 35.15395583227016 37.0 35.0 37.0 33.0 37.0 6 35.22766030444217 37.0 35.0 37.0 33.0 37.0 7 35.14772826850558 37.0 35.0 37.0 32.0 37.0 8 35.132391797379164 37.0 35.0 37.0 32.0 37.0 9 36.70093881303496 39.0 37.0 39.0 32.0 39.0 10-11 36.59049646738363 39.0 37.0 39.0 32.0 39.0 12-13 36.48073287122919 39.0 37.0 39.0 32.0 39.0 14-15 37.60272881061404 40.0 37.5 41.0 32.0 41.0 16-17 37.49466580127344 40.0 37.0 41.0 32.0 41.0 18-19 37.47856666523094 40.0 37.0 41.0 32.0 41.0 20-21 37.39194887248867 40.0 37.0 41.0 32.0 41.0 22-23 37.34332768451367 40.0 37.0 41.0 32.0 41.0 24-25 37.245922676025586 40.0 37.0 41.0 31.5 41.0 26-27 37.08282823182043 40.0 36.5 41.0 31.0 41.0 28-29 36.88517622980646 40.0 36.0 41.0 30.5 41.0 30-31 36.5954898061331 39.0 36.0 41.0 30.0 41.0 32-33 36.31103732825187 39.0 35.0 41.0 30.0 41.0 34-35 35.95686153361741 39.0 35.0 41.0 29.5 41.0 36-37 35.6903409639431 39.0 35.0 41.0 28.0 41.0 38-39 35.465756568052434 38.0 35.0 40.0 27.0 41.0 40-41 35.26460908039097 38.5 35.0 40.5 26.0 41.0 42-43 35.135478845145606 39.0 35.0 41.0 25.0 41.0 44-45 34.83881704507817 39.0 35.0 41.0 24.0 41.0 46-47 34.37452194202558 38.0 34.0 41.0 21.5 41.0 48-49 33.9558055360413 38.0 34.0 40.5 16.5 41.0 50-51 32.75693787437045 37.0 32.5 39.5 11.0 40.5 52-53 32.789514137148984 37.5 32.5 39.5 9.0 40.5 54-55 33.24211974804581 38.0 33.0 40.0 7.5 41.0 56-57 33.11709245696922 38.0 33.0 40.0 6.0 41.0 58-59 32.890816385636654 37.5 33.0 40.0 2.0 41.0 60-61 32.58421989107333 37.0 33.0 40.0 2.0 41.0 62-63 32.17519163162765 36.5 32.0 40.0 2.0 41.0 64-65 31.777707311929206 36.0 31.5 39.0 2.0 41.0 66-67 31.37942393921256 35.5 31.0 39.0 2.0 41.0 68-69 30.92870976701218 35.0 31.0 39.0 2.0 40.0 70-71 30.482342116480545 35.0 30.0 38.0 2.0 40.0 72-73 30.041382183494058 35.0 30.0 37.0 2.0 40.0 74-75 29.55603322159463 35.0 29.0 37.0 2.0 39.0 76-77 29.074129247568308 34.5 29.0 36.0 2.0 39.0 78-79 28.600232141239303 34.0 28.5 36.0 2.0 38.5 80-81 28.093218891514443 34.0 27.0 35.0 2.0 37.0 82-83 27.647494344991884 34.0 26.0 35.0 2.0 37.0 84-85 27.2621012718013 34.0 26.0 35.0 2.0 36.0 86-87 26.783128396532287 34.0 25.0 35.0 2.0 36.0 88-89 26.27647977044306 33.5 23.5 35.0 2.0 35.5 90-91 25.7688204462518 33.0 20.5 35.0 2.0 35.0 92-93 25.252378965003558 33.0 18.5 35.0 2.0 35.0 94-95 24.679673309104302 32.5 5.0 35.0 2.0 35.0 96-97 24.046696331633736 32.0 2.0 35.0 2.0 35.0 98-99 23.27468338638075 32.0 2.0 35.0 2.0 35.0 100-101 21.068114814116207 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1521316444150287 1101 2 0.07900801399992474 1101 3 0.4182618880308162 1101 4 0.2513562953749826 1101 5 0.1032686277031587 1101 6 0.10578234874004977 1101 7 0.21651155305268333 1101 8 0.29840432548812856 1101 9 0.3974876478249314 1101 10-11 0.3830234942305353 1101 12-13 0.28739969498993645 1101 14-15 0.24813191002913726 1101 16-17 0.4202663538180573 1101 18-19 0.41289447897653986 1101 20-21 0.4109519251076179 1101 22-23 0.3361831554915966 1101 24-25 0.37292781074197734 1101 26-27 0.4230891441402207 1101 28-29 0.41912185186833995 1101 30-31 0.2949748104450052 1101 32-33 0.14410251965679066 1101 34-35 0.1125917757971493 1101 36-37 0.1718153568630285 1101 38-39 0.41497245023989393 1101 40-41 0.5680074274492171 1101 42-43 0.5978434530451864 1101 44-45 0.5797075429510912 1101 46-47 0.6132766052930165 1101 48-49 0.4123431996745168 1101 50-51 0.26309812427414414 1101 52-53 0.2937412989215815 1101 54-55 0.26430543457346545 1101 56-57 0.4166219160852904 1101 58-59 -0.048436538047681665 1101 60-61 -0.003785581901034618 1101 62-63 -0.12866031864180627 1101 64-65 -0.3426355538628947 1101 66-67 -0.15793552709166292 1101 68-69 -0.17639154363122955 1101 70-71 -0.03695149321355373 1101 72-73 -0.25021351186710206 1101 74-75 -0.2730012119575207 1101 76-77 -0.7134320655212107 1101 78-79 -0.6324996103424851 1101 80-81 -0.3054279532194677 1101 82-83 -0.05244688042894552 1101 84-85 -0.39476642499671044 1101 86-87 -0.626860757824474 1101 88-89 -0.7966383922994318 1101 90-91 -0.7216942249452423 1101 92-93 -0.4632117670147089 1101 94-95 -0.5998406984709597 1101 96-97 -0.723807632150848 1101 98-99 -0.6692335783235173 1101 100-101 -0.7274907624750853 1102 1 0.01677083999862461 1102 2 0.15146463860560289 1102 3 -0.05666417663795542 1102 4 0.09119265184186531 1102 5 0.10607817028991207 1102 6 -0.05634304558171266 1102 7 0.08619806724826873 1102 8 0.009331967443962697 1102 9 0.1984882393075793 1102 10-11 0.23407337593400968 1102 12-13 0.1981967178606041 1102 14-15 0.2607699226474338 1102 16-17 0.4026278482975485 1102 18-19 0.3493076296074591 1102 20-21 0.279457938514561 1102 22-23 0.5797409236303963 1102 24-25 0.3981915134233631 1102 26-27 0.4512535763168728 1102 28-29 0.44307936369799705 1102 30-31 0.5391437773219891 1102 32-33 0.504556975492747 1102 34-35 0.36774013936182115 1102 36-37 0.2528879120680756 1102 38-39 0.35567385008217656 1102 40-41 0.5321463272914997 1102 42-43 0.5185453457896685 1102 44-45 0.5258653702381579 1102 46-47 0.3825441526116222 1102 48-49 0.4508585782234107 1102 50-51 0.5413808529807724 1102 52-53 0.46230892510454424 1102 54-55 0.3784763730592644 1102 56-57 0.4963882804386017 1102 58-59 0.29273311889237874 1102 60-61 0.1494651081620546 1102 62-63 0.22312152284084164 1102 64-65 -0.15204259250642949 1102 66-67 -0.037936019993868086 1102 68-69 0.3065762205643523 1102 70-71 0.09279761956247512 1102 72-73 -0.2039472460942342 1102 74-75 -0.3532580140079915 1102 76-77 -0.03022770826568788 1102 78-79 -0.06269479961693492 1102 80-81 0.09645000419378036 1102 82-83 0.3208649295079624 1102 84-85 0.19067570828404712 1102 86-87 0.29646091015660403 1102 88-89 0.002942640823597742 1102 90-91 0.12184629161627214 1102 92-93 -0.1938628908317419 1102 94-95 -0.10049379389682755 1102 96-97 0.0926405145368463 1102 98-99 -0.541571906399227 1102 100-101 -0.2551992601091513 1103 1 0.01852824935330233 1103 2 0.1465234460986906 1103 3 0.1314370423518021 1103 4 0.18457388796758067 1103 5 0.22460350424636744 1103 6 0.3080200030610385 1103 7 0.3906242073736692 1103 8 0.30976620820418077 1103 9 0.11799304905949981 1103 10-11 0.19770327818115874 1103 12-13 0.1568537844961142 1103 14-15 0.22598684830074234 1103 16-17 0.22465485690447196 1103 18-19 0.3008863771246908 1103 20-21 0.29005107634218774 1103 22-23 0.36799256907183775 1103 24-25 0.24197680765555418 1103 26-27 0.28038081080688926 1103 28-29 0.18622254631278423 1103 30-31 0.4219231129141434 1103 32-33 0.4627174887925918 1103 34-35 0.4775319764144328 1103 36-37 0.4407968383445109 1103 38-39 0.2595615706102663 1103 40-41 0.23773157868379258 1103 42-43 0.4963002431686405 1103 44-45 0.4519008299881264 1103 46-47 0.6274934262806724 1103 48-49 0.7283540021436536 1103 50-51 0.6503280625457535 1103 52-53 0.4277497865759017 1103 54-55 0.5297182432154344 1103 56-57 0.5666797864556585 1103 58-59 0.7369511934337964 1103 60-61 0.7926939705681022 1103 62-63 0.5322868264199201 1103 64-65 0.4246773319395736 1103 66-67 0.5810980377231552 1103 68-69 0.6001998915539524 1103 70-71 0.45042312098398085 1103 72-73 0.7552745282563507 1103 74-75 0.5199675380424793 1103 76-77 0.4218013449726179 1103 78-79 0.3872920563241813 1103 80-81 0.48872713937312895 1103 82-83 0.6544879189537696 1103 84-85 0.5961382817934116 1103 86-87 0.6274891650150352 1103 88-89 0.9357497249845181 1103 90-91 0.6951171176473494 1103 92-93 0.4282137731087481 1103 94-95 0.6350647799241038 1103 96-97 0.6846221517997684 1103 98-99 0.4399485204419129 1103 100-101 0.7597874011051644 1104 1 0.26610935759332577 1104 2 0.32043243260457643 1104 3 0.03234861283701562 1104 4 0.2512836209563787 1104 5 0.24050215483494242 1104 6 0.344784286724547 1104 7 0.5157605840604305 1104 8 0.47317418982921566 1104 9 0.41191351991795244 1104 10-11 0.5123960717499187 1104 12-13 0.33682435584264425 1104 14-15 0.30069973948649675 1104 16-17 0.3460839410273522 1104 18-19 0.44585838401529543 1104 20-21 0.44152968673552806 1104 22-23 0.3304782136311246 1104 24-25 0.26928803361018794 1104 26-27 0.48852034569060265 1104 28-29 0.6100920553567093 1104 30-31 0.5789501011426879 1104 32-33 0.4401902134552387 1104 34-35 0.3387363978901803 1104 36-37 0.4400324111265945 1104 38-39 0.48473383589881536 1104 40-41 0.6464776309375964 1104 42-43 0.6460023677738675 1104 44-45 0.6543017774472091 1104 46-47 0.5881918184713086 1104 48-49 0.5084430058760674 1104 50-51 0.7563394033439153 1104 52-53 0.8608318996967697 1104 54-55 0.41342122914710444 1104 56-57 0.34223964864343515 1104 58-59 0.30827978947169754 1104 60-61 0.48993413709121114 1104 62-63 0.650500291823306 1104 64-65 0.6397469558270323 1104 66-67 0.5656049283346967 1104 68-69 0.6229604428028743 1104 70-71 0.7835124118252104 1104 72-73 0.43346502495460015 1104 74-75 0.35824273184092803 1104 76-77 0.4233230216880912 1104 78-79 0.2096757772544109 1104 80-81 0.45427408166425565 1104 82-83 0.2265199315865587 1104 84-85 0.5202590110498022 1104 86-87 0.3719946200825035 1104 88-89 0.27088825498739055 1104 90-91 -0.011235164867724023 1104 92-93 0.1003965178690116 1104 94-95 0.07459607478485353 1104 96-97 0.3408847128103929 1104 98-99 0.6225784371803584 1104 100-101 0.9654900999280187 1105 1 -0.043246349596945066 1105 2 0.09940472058675098 1105 3 0.04622783114458784 1105 4 0.1353944690276805 1105 5 -0.04091176151840159 1105 6 -0.07434489676892753 1105 7 -0.14144129125869398 1105 8 -0.009576587685522497 1105 9 -0.1352874270253679 1105 10-11 0.09513988644610549 1105 12-13 0.13533663361268822 1105 14-15 0.10557951481955286 1105 16-17 0.05028600202164313 1105 18-19 0.0812561930483966 1105 20-21 0.25084245654473847 1105 22-23 0.15563487704847745 1105 24-25 0.16896542301741846 1105 26-27 0.03661833575698381 1105 28-29 0.04230205396414988 1105 30-31 0.2843741367923158 1105 32-33 0.1289359777406176 1105 34-35 0.0466252713061337 1105 36-37 0.2403782311145335 1105 38-39 0.11374209844348826 1105 40-41 0.10159182116179721 1105 42-43 0.06782848298529842 1105 44-45 0.31718415223252094 1105 46-47 0.506979823855886 1105 48-49 0.4470924511715211 1105 50-51 0.31772013026217394 1105 52-53 0.32133299053834463 1105 54-55 0.4075782639147789 1105 56-57 0.22026151189367482 1105 58-59 0.2438679155451311 1105 60-61 0.3718366112127356 1105 62-63 0.5038668145917242 1105 64-65 0.3508758608077649 1105 66-67 0.19779519895624276 1105 68-69 -0.14262002417015296 1105 70-71 0.10468866397207677 1105 72-73 0.1935692351388809 1105 74-75 0.02707831648559278 1105 76-77 0.09788436411950485 1105 78-79 0.35123916211260564 1105 80-81 0.332511792289516 1105 82-83 0.07678683963093391 1105 84-85 0.32435314835658247 1105 86-87 0.1753332915767274 1105 88-89 0.5616044969221257 1105 90-91 0.6107580579888889 1105 92-93 0.4503408153206223 1105 94-95 0.44471862039131693 1105 96-97 0.28527732293897046 1105 98-99 0.6438277989219934 1105 100-101 1.0159925833333006 1106 1 0.16389500459072437 1106 2 0.11529095689889957 1106 3 0.2670745710469191 1106 4 0.2987894944965106 1106 5 0.21488557499451844 1106 6 0.39589032824224546 1106 7 0.23837740224740855 1106 8 0.25367290162282785 1106 9 0.16179264416460626 1106 10-11 0.3003436809069626 1106 12-13 0.42291740179813786 1106 14-15 0.5006849846997099 1106 16-17 0.6396077246086875 1106 18-19 0.3773904983762506 1106 20-21 0.5876940096610568 1106 22-23 0.3923393597375622 1106 24-25 0.3754316998342091 1106 26-27 0.1221328849894121 1106 28-29 0.03817268459162193 1106 30-31 -0.03824490038954309 1106 32-33 0.30339745011067265 1106 34-35 0.4574295777737305 1106 36-37 0.6817714329977349 1106 38-39 0.39120437739948954 1106 40-41 0.19725225724424433 1106 42-43 0.19394520999979648 1106 44-45 0.09284695546939048 1106 46-47 0.2523025020719345 1106 48-49 0.3928199566291255 1106 50-51 0.31420994711455563 1106 52-53 0.1330554460665283 1106 54-55 0.04065289064959643 1106 56-57 0.33155831798865876 1106 58-59 0.5203855245438263 1106 60-61 0.6115340099730489 1106 62-63 0.5751521356041636 1106 64-65 0.5141371858881989 1106 66-67 0.6109940574617774 1106 68-69 0.6329868879207474 1106 70-71 0.43114994346287716 1106 72-73 0.3952694218810642 1106 74-75 0.27152317493852607 1106 76-77 0.2793651068799612 1106 78-79 0.3429716561289631 1106 80-81 0.24093688938668478 1106 82-83 0.2822952956325473 1106 84-85 0.330630491181914 1106 86-87 0.6727743538153526 1106 88-89 0.6571980828103783 1106 90-91 0.6140545396960455 1106 92-93 0.7210271147568896 1106 94-95 0.9291501964777957 1106 96-97 0.7304360537935146 1106 98-99 0.7850581493485187 1106 100-101 0.7799248213462917 1107 1 0.10831786890482675 1107 2 0.11809329096785603 1107 3 0.2401141110628835 1107 4 0.24868683473358288 1107 5 0.16790966268857943 1107 6 0.12916062862343125 1107 7 0.08056949765909849 1107 8 0.03030574677092801 1107 9 0.2438478883497126 1107 10-11 0.213922728924409 1107 12-13 0.1519564551940178 1107 14-15 0.03710785755099977 1107 16-17 0.19053102218540374 1107 18-19 0.2750049928249396 1107 20-21 -0.14058096483407923 1107 22-23 -0.13590594800695044 1107 24-25 -0.13698244800438175 1107 26-27 -0.1129622406994315 1107 28-29 -0.47196096518705843 1107 30-31 -0.1846033601089232 1107 32-33 0.017694903038709242 1107 34-35 0.17621012492251964 1107 36-37 -0.12395723788915802 1107 38-39 -0.13522570938109624 1107 40-41 -0.23790775112220786 1107 42-43 -0.0030676256720099104 1107 44-45 0.17233463843214025 1107 46-47 0.40386971753790846 1107 48-49 0.42758554661329384 1107 50-51 0.22547968404090568 1107 52-53 0.28545503798863336 1107 54-55 0.3780855001711316 1107 56-57 0.36868550937975186 1107 58-59 0.5461038227394823 1107 60-61 0.28328848879866797 1107 62-63 0.25171732348646714 1107 64-65 0.47260907077267333 1107 66-67 0.3112905115380791 1107 68-69 0.4897231611792634 1107 70-71 0.34764444337536915 1107 72-73 0.24388042034863844 1107 74-75 0.5070302202582226 1107 76-77 0.4024639803971546 1107 78-79 0.48902917974497484 1107 80-81 0.5018196779758703 1107 82-83 0.34083163633490443 1107 84-85 0.5678588483269031 1107 86-87 0.6205267239539545 1107 88-89 0.7733370085752043 1107 90-91 0.8900609682034464 1107 92-93 1.0840937176791705 1107 94-95 1.0816575704794786 1107 96-97 0.9244203197441934 1107 98-99 1.0557117569534533 1107 100-101 1.501880137670689 1108 1 0.3187487079070941 1108 2 0.19259928384119362 1108 3 0.011639864221290708 1108 4 0.14515589854958932 1108 5 0.31750473881427155 1108 6 0.1656037773114818 1108 7 0.18312861654474233 1108 8 0.26117714294844774 1108 9 0.18699657639636058 1108 10-11 0.2141494862940263 1108 12-13 0.36318094498993503 1108 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-0.12190072000306174 2316 80-81 -0.020314539381345753 2316 82-83 0.11690894528906881 2316 84-85 0.13385425059075473 2316 86-87 -0.02973893993935306 2316 88-89 0.013907462791955538 2316 90-91 -0.23461646711234252 2316 92-93 -0.2459936697562206 2316 94-95 -0.09305830617330813 2316 96-97 -0.15356040630228307 2316 98-99 -0.474961385112028 2316 100-101 -0.6845260921095466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5690.0 3 1044.0 4 882.0 5 836.0 6 989.0 7 1144.0 8 1519.0 9 1837.0 10 2175.0 11 2961.0 12 4083.0 13 5314.0 14 5757.0 15 6920.0 16 8115.0 17 10221.0 18 8796.0 19 5831.0 20 5631.0 21 5862.0 22 6420.0 23 6855.0 24 7788.0 25 8753.0 26 10030.0 27 11521.0 28 13336.0 29 15324.0 30 18064.0 31 21603.0 32 25787.0 33 32012.0 34 41743.0 35 58581.0 36 80372.0 37 113105.0 38 100035.0 39 16358.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80370711249713 19.916868463928182 14.639311595821681 21.640112827753004 2 16.083470819876016 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21.35056864011525 26.784645972789807 32.084264696690546 19.7805206904044 22-23 21.072806347533092 26.704916836774956 31.971871459179223 20.250405356512726 24-25 20.699486011396328 26.98615096600827 32.29009650778055 20.024266514814855 26-27 20.31078723675376 27.05166448451392 32.229290724596424 20.408257554135893 28-29 20.250862680358303 27.390399365877787 32.191535898826515 20.16720205493739 30-31 20.485126621638027 27.25766942900914 31.808273025789063 20.448930923563772 32-33 20.119213172229625 27.49879276387503 31.69186423299769 20.690129830897654 34-35 20.832631498924517 27.414248257090517 31.562723127443686 20.19039711654128 36-37 20.860929374266064 27.070476616525934 31.42746369923018 20.641130309977818 38-39 20.436038190427347 27.278812781031213 31.565470581920973 20.719678446620467 40-41 20.651539730004227 27.225579927586825 31.501593492203178 20.621286850205774 42-43 20.47745520223566 27.432057003613043 30.934105785264425 21.156382008886876 44-45 20.423124777751024 27.49289735135131 30.948016290430676 21.13596158046699 46-47 20.749834493370788 27.702753076037624 30.310153458939443 21.237258971652142 48-49 20.621665454952183 27.81887664746565 30.028671028838804 21.53078686874336 50-51 20.605213319614073 28.041127961943964 29.79450931269479 21.559149405747178 52-53 20.754503144260177 28.070332410261457 29.596458028563035 21.57870641691533 54-55 20.58299651890075 27.921264081154572 29.61894302523853 21.87679637470615 56-57 20.562271208347898 28.18191372872904 29.30754443151587 21.94827063140719 58-59 20.506813024669967 27.70488443753889 29.575643762831476 22.212658774959664 60-61 20.37571339282588 27.8228603057467 29.82754908731161 21.97387721411581 62-63 20.489659115730085 27.61858116343614 29.905406422880777 21.986353297952995 64-65 20.372894804350288 27.668743773154915 29.710626950083817 22.247734472410976 66-67 20.25491472639287 27.784303153285173 29.419226664697867 22.541555455624092 68-69 20.414572536396662 27.493360559823575 29.562792584709264 22.5292743190705 70-71 20.398432937838823 27.405688766777565 29.399643361352812 22.7962349340308 72-73 20.303151652635872 27.288868804060318 29.346171714844154 23.061807828459653 74-75 20.109974676295046 27.596872865456255 29.15372890809792 23.139423550150777 76-77 20.105022844668184 27.212667159745667 29.45277177997542 23.229538215610724 78-79 20.09707934581759 27.2642840584593 29.4451405541066 23.193496041616505 80-81 20.145560863316216 27.158514184841277 29.233311863739647 23.462613088102863 82-83 20.183822076691442 26.885693474301362 29.355989016601097 23.574495432406096 84-85 20.116709150865255 26.59155450415759 29.434056794874756 23.8576795501024 86-87 20.101569465940234 26.487831643987985 29.471919293129 23.938679596942777 88-89 20.17898269297154 26.24937837698798 29.38034530552538 24.191293624515097 90-91 20.140719031745675 26.11355958746281 29.496185596993225 24.249535783798297 92-93 20.38433487500802 25.917957651424423 29.44466899235327 24.25303848121429 94-95 20.22413922973488 25.597991741139847 29.56685358608598 24.611015443039296 96-97 20.240709031137946 25.189354390277373 29.688665286470297 24.881271292114384 98-99 20.29540081587602 25.216460708932125 29.549180022224046 24.938958452967803 100-101 20.628752537727614 24.573628210509675 30.06302287626775 24.734596375494963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2149.0 1 1430.0 2 771.5 3 860.5 4 778.5 5 738.0 6 1038.5 7 1610.0 8 2259.0 9 2484.5 10 2061.0 11 1675.0 12 1670.0 13 1702.0 14 1761.0 15 1821.0 16 1837.0 17 1975.5 18 2196.5 19 2342.0 20 2486.5 21 2766.5 22 3059.0 23 3518.5 24 4012.0 25 4568.5 26 5570.0 27 6729.5 28 7567.5 29 8376.0 30 9364.0 31 10623.5 32 12277.0 33 13426.5 34 14222.0 35 15382.5 36 16940.5 37 18269.0 38 18823.0 39 19090.0 40 19430.0 41 19750.0 42 20055.5 43 20804.5 44 21758.5 45 22428.5 46 22580.0 47 23053.5 48 24518.5 49 24348.5 50 22678.0 51 21638.0 52 21006.5 53 20414.5 54 19416.5 55 17950.5 56 16311.5 57 14469.0 58 13086.5 59 11892.5 60 10179.0 61 8425.5 62 6902.5 63 5174.5 64 3640.5 65 2779.5 66 2101.5 67 1677.5 68 1310.5 69 963.0 70 737.5 71 553.5 72 448.0 73 385.5 74 291.0 75 236.0 76 194.5 77 145.5 78 104.5 79 68.0 80 57.5 81 45.5 82 35.0 83 32.0 84 15.5 85 10.0 86 10.0 87 6.5 88 4.0 89 1.5 90 1.0 91 0.5 92 0.5 93 2.5 94 2.5 95 1.5 96 1.0 97 0.0 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27194536734903024 2 0.16486038107996917 3 0.19129745119909936 4 0.16159287803153735 5 0.23377499082871306 6 0.19382233991834213 7 0.23659692527963141 8 0.24595386582741346 9 0.15149332315456626 10-11 0.2068180906791505 12-13 0.21223917528223057 14-15 0.17399453732899448 16-17 0.28724322253032464 18-19 0.23191845500574038 20-21 0.1994662088201789 22-23 0.24743909448579157 24-25 0.2360770952491991 26-27 0.3450928787741517 28-29 0.3167992728320489 30-31 0.2890997583532973 32-33 0.19419364708293665 34-35 0.3618017011809053 36-37 0.3987096333416011 38-39 0.37917887648392906 40-41 0.33959753273815274 42-43 0.37613415773425396 44-45 0.3856396211478738 46-47 0.39046661428760265 48-49 0.40984884827943685 50-51 0.4020513978229518 52-53 0.4024969664204653 54-55 0.39737292754906084 56-57 0.3739063147466868 58-59 0.3474692446275567 60-61 0.31494273700907627 62-63 0.3840058696236579 64-65 0.4177948216017597 66-67 0.42143363181478605 68-69 0.4264834092532716 70-71 0.4263348863874338 72-73 0.4169779458396517 74-75 0.4127450441632741 76-77 0.3981898033111688 78-79 0.3721240403566331 80-81 0.38333751672738775 82-83 0.39336281017144 84-85 0.3944024702323046 86-87 0.3895754770925758 88-89 0.3970758818173852 90-91 0.4176462987359219 92-93 0.41794334446759757 94-95 0.42559227205824474 96-97 0.42492391916197453 98-99 0.4235872133694343 100-101 0.3961847446223583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 673297.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.28471990293485 #Duplication Level Percentage of deduplicated Percentage of total 1 84.97917468738225 56.32820791735704 2 8.99391949062758 11.92318868531591 3 2.4533123581392124 4.87851367480999 4 1.0823000798792515 2.8695983058288084 5 0.6038198109099143 2.001201351900338 6 0.38878699247892406 1.5462382139021953 7 0.25694186261453866 1.1921923576320146 8 0.20296488229102064 1.0762776298233956 9 0.14890420099436616 0.8883065929753688 >10 0.8211939834982159 9.974584865967596 >50 0.04556341482670343 2.060548675671299 >100 0.020873747391562805 2.2590044966179024 >500 0.0011222444832808263 0.5637096968531027 >1k 8.97795586624661E-4 1.63236283818217 >5k 2.2444889665616525E-4 0.8060646971628689 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5418 0.8046968871092549 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4435 0.6586989099906876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2833 0.4207652789185159 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2469 0.36670295575355305 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1235 0.1834257393096954 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.1476317286427832 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.1225313643161933 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 774 0.11495669815846499 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4852286583781006E-4 2 0.0 0.0 0.0 0.0 1.4852286583781006E-4 3 0.0 0.0 0.0 0.0 1.4852286583781006E-4 4 0.0 0.0 0.0 0.0 1.4852286583781006E-4 5 0.0 0.0 0.0 2.970457316756201E-4 1.4852286583781006E-4 6 0.0 0.0 0.0 2.970457316756201E-4 1.4852286583781006E-4 7 0.0 0.0 0.0 2.970457316756201E-4 1.4852286583781006E-4 8 0.0 0.0 0.0 2.970457316756201E-4 1.4852286583781006E-4 9 0.0 0.0 0.0 2.970457316756201E-4 1.4852286583781006E-4 10-11 0.0 1.4852286583781006E-4 0.0 2.970457316756201E-4 1.4852286583781006E-4 12-13 0.0 1.4852286583781006E-4 0.0 2.970457316756201E-4 1.4852286583781006E-4 14-15 0.0 1.4852286583781006E-4 0.0 2.970457316756201E-4 1.4852286583781006E-4 16-17 0.0 1.4852286583781006E-4 0.0 2.970457316756201E-4 1.4852286583781006E-4 18-19 0.0 1.4852286583781006E-4 0.0 2.970457316756201E-4 1.4852286583781006E-4 20-21 0.0 1.4852286583781006E-4 0.0 5.198300304323352E-4 1.4852286583781006E-4 22-23 0.0 1.4852286583781006E-4 0.0 0.0015594900912970055 1.4852286583781006E-4 24-25 0.0 1.4852286583781006E-4 0.0 0.004901254572647732 1.4852286583781006E-4 26-27 0.0 1.4852286583781006E-4 0.0 0.009951032011133273 1.4852286583781006E-4 28-29 0.0 1.4852286583781006E-4 0.0 0.026362808686211287 1.4852286583781006E-4 30-31 7.426143291890503E-5 1.4852286583781006E-4 0.0 0.0810934847474443 1.4852286583781006E-4 32-33 1.4852286583781006E-4 1.4852286583781006E-4 0.0 0.17481141309110243 1.4852286583781006E-4 34-35 1.4852286583781006E-4 1.4852286583781006E-4 0.0 0.32749291917237117 1.4852286583781006E-4 36-37 1.4852286583781006E-4 1.4852286583781006E-4 0.0 0.5464898848502221 1.4852286583781006E-4 38-39 1.4852286583781006E-4 1.4852286583781006E-4 0.0 0.8545263085978402 1.4852286583781006E-4 40-41 1.4852286583781006E-4 1.4852286583781006E-4 0.0 1.1791972933192931 1.4852286583781006E-4 42-43 1.4852286583781006E-4 1.4852286583781006E-4 0.0 1.494288553194207 1.4852286583781006E-4 44-45 1.4852286583781006E-4 1.4852286583781006E-4 0.0 1.8802252200737564 1.4852286583781006E-4 46-47 1.4852286583781006E-4 1.4852286583781006E-4 0.0 2.332997176580321 1.4852286583781006E-4 48-49 1.4852286583781006E-4 1.4852286583781006E-4 0.0 2.7833927672334795 1.4852286583781006E-4 50-51 1.4852286583781006E-4 1.4852286583781006E-4 0.0 3.258368892182796 1.4852286583781006E-4 52-53 1.4852286583781006E-4 1.4852286583781006E-4 0.0 3.725176259511033 1.4852286583781006E-4 54-55 1.4852286583781006E-4 1.4852286583781006E-4 0.0 4.243001231254558 1.4852286583781006E-4 56-57 1.4852286583781006E-4 1.4852286583781006E-4 0.0 4.827438708326341 1.4852286583781006E-4 58-59 1.4852286583781006E-4 1.4852286583781006E-4 0.0 5.441729281431522 1.4852286583781006E-4 60-61 1.4852286583781006E-4 1.4852286583781006E-4 0.0 6.095452675416643 1.4852286583781006E-4 62-63 1.4852286583781006E-4 1.4852286583781006E-4 0.0 6.738333900195604 1.4852286583781006E-4 64-65 1.4852286583781006E-4 1.4852286583781006E-4 0.0 7.4293365335060155 1.4852286583781006E-4 66-67 1.4852286583781006E-4 1.4852286583781006E-4 0.0 8.184129737693766 2.2278429875671507E-4 68-69 1.4852286583781006E-4 1.4852286583781006E-4 0.0 9.005832492941451 2.970457316756201E-4 70-71 1.4852286583781006E-4 1.4852286583781006E-4 0.0 9.892068433395664 2.970457316756201E-4 72-73 1.4852286583781006E-4 1.4852286583781006E-4 0.0 10.770506923393391 2.970457316756201E-4 74-75 1.4852286583781006E-4 1.4852286583781006E-4 0.0 11.66513440576744 2.970457316756201E-4 76-77 1.4852286583781006E-4 1.4852286583781006E-4 0.0 12.59429345444878 2.970457316756201E-4 78-79 1.4852286583781006E-4 1.4852286583781006E-4 0.0 13.603209282085023 2.970457316756201E-4 80-81 1.4852286583781006E-4 1.4852286583781006E-4 0.0 14.668712321605472 2.970457316756201E-4 82-83 1.4852286583781006E-4 1.4852286583781006E-4 0.0 15.749736000605973 2.970457316756201E-4 84-85 1.4852286583781006E-4 1.4852286583781006E-4 0.0 16.82155126192453 2.970457316756201E-4 86-87 1.4852286583781006E-4 1.4852286583781006E-4 0.0 17.934878664244756 2.970457316756201E-4 88-89 1.4852286583781006E-4 1.4852286583781006E-4 0.0 19.083034678603944 2.970457316756201E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3430 0.0 40.03051 1 GTATCAA 5090 0.0 31.735731 1 CTACCGT 65 0.0058810217 29.191341 9 ATAGCGT 70 0.0084445365 27.106245 6 AATAGCG 70 0.0084445365 27.106245 5 TATCAAC 6275 0.0 25.777933 2 ATCAACG 6435 0.0 25.210703 3 TCAACGC 6475 0.0 25.128223 4 CAACGCA 6520 0.0 24.882038 5 AACGCAG 6670 0.0 24.322472 6 GTACATG 3470 0.0 23.27592 1 GTCTTAG 205 2.6589987E-8 23.175735 1 TACATGG 3430 0.0 22.680738 2 GTCTAGC 105 0.0021925191 22.623934 1 GTATAGG 150 3.0257293E-5 22.171455 1 GAGTACT 4305 0.0 22.002174 12-13 ACGCAGA 7355 0.0 21.929869 7 CGCAGAG 7390 0.0 21.697617 8 ACATGGG 3470 0.0 21.188961 3 GCAGAGT 7620 0.0 20.356365 9 >>END_MODULE