FastQCFastQC Report
Thu 26 May 2016
SRR938838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673297
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50840.7550902499194263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44360.6588474328565255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.44527155178175454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24670.3664059100218774No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT21480.319027115819616No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.1875843795531541No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT11450.1700586813842925TruSeq Adapter, Index 10 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.15906798931229457No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.1268385274254898No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7460.11079805791500631TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.043.2102931
GTATCAA49250.032.9412841
TATCAAC62000.026.02252
ATCAACG62450.025.7593
TCAACGC62850.025.5950624
CAACGCA62850.025.519565
AACGCAG64200.024.903476
GATATAG1652.3936827E-623.0673891
GCATATA1252.2301183E-422.8367141
CTATACC1050.002208004522.596574
GTACATG35300.022.373081
ACGCAGA72400.021.82087
ACGCAGT1100.00289652521.5646468
CGCAGAG72650.021.5498058
TACATGG35600.021.4603922
GAGTACT45500.021.37665412-13
ACATGGG34400.020.8295693
AGTACTT46750.020.5006212-13
GCAGAGT76200.020.1722819
GTACTTT49100.019.80932414-15
Produced by FastQC (version 0.11.5)