##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938838_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673297 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968039364500363 31.0 31.0 34.0 30.0 34.0 2 32.166229761903 33.0 31.0 34.0 30.0 34.0 3 32.222099608345204 34.0 31.0 34.0 30.0 34.0 4 35.89175059446277 37.0 35.0 37.0 35.0 37.0 5 35.796141970037 37.0 35.0 37.0 35.0 37.0 6 35.82879917777741 37.0 35.0 37.0 35.0 37.0 7 35.735121350607535 37.0 35.0 37.0 33.0 37.0 8 35.73390197787901 37.0 35.0 37.0 33.0 37.0 9 37.34547161208204 39.0 37.0 39.0 34.0 39.0 10-11 37.287142969595884 39.0 37.0 39.0 33.5 39.0 12-13 37.21061804820161 39.0 37.0 39.0 33.0 39.0 14-15 38.36612223134813 40.0 38.0 41.0 33.0 41.0 16-17 38.30442434765044 40.0 38.0 41.0 33.0 41.0 18-19 38.43748895361185 40.0 38.0 41.0 33.5 41.0 20-21 38.444153917216326 40.0 38.0 41.0 34.0 41.0 22-23 38.35011517948246 40.0 38.0 41.0 33.5 41.0 24-25 38.243331694631046 40.0 37.5 41.0 33.0 41.0 26-27 38.01508621009748 40.0 37.0 41.0 33.0 41.0 28-29 37.78768953374217 40.0 37.0 41.0 33.0 41.0 30-31 37.47994421481159 39.5 36.5 41.0 32.0 41.0 32-33 37.210041036867835 39.0 36.0 41.0 31.5 41.0 34-35 37.08486299508241 40.0 35.0 41.0 31.0 41.0 36-37 36.885357427702786 39.5 35.0 41.0 31.0 41.0 38-39 36.67233627953192 39.0 35.0 41.0 30.5 41.0 40-41 36.43237011304075 39.0 35.0 41.0 30.0 41.0 42-43 36.14882436725546 39.0 35.0 41.0 30.0 41.0 44-45 35.76997372630503 39.0 35.0 41.0 28.5 41.0 46-47 35.2891354038411 39.0 35.0 41.0 26.5 41.0 48-49 34.82929969983529 39.0 34.5 40.5 24.0 41.0 50-51 34.49355336500831 38.0 34.0 40.0 23.0 41.0 52-53 34.23543918954043 38.0 34.0 40.0 22.5 41.0 54-55 33.926992100068766 38.0 34.0 40.0 20.5 41.0 56-57 33.60424077338827 38.0 33.0 40.0 19.5 41.0 58-59 33.24183532675773 37.0 33.0 40.0 16.5 41.0 60-61 32.83295187710624 37.0 32.5 40.0 12.0 41.0 62-63 32.43689560476283 36.0 32.0 39.5 8.0 41.0 64-65 31.997397136776193 36.0 31.5 39.0 4.5 40.5 66-67 31.555484429605357 35.0 31.0 39.0 2.0 40.0 68-69 31.200918762448072 35.0 31.0 38.5 2.0 40.0 70-71 30.781244383979136 35.0 30.5 37.5 2.0 40.0 72-73 30.302507660066805 35.0 30.0 37.0 2.0 39.5 74-75 29.8219983157507 34.5 29.5 36.5 2.0 39.0 76-77 27.91276063906419 32.5 26.5 34.5 2.0 36.5 78-79 28.80643089156791 34.0 29.0 35.0 2.0 37.0 80-81 28.838540049933385 34.0 29.0 35.0 2.0 37.0 82-83 28.509724534640732 34.0 29.0 35.0 2.0 37.0 84-85 28.1107037458952 34.0 28.5 35.0 2.0 36.0 86-87 27.553079101793116 34.0 26.5 35.0 2.0 36.0 88-89 26.965692703220125 33.0 25.5 35.0 2.0 35.0 90-91 26.450281970660793 33.0 24.5 35.0 2.0 35.0 92-93 26.145341506051565 33.0 24.0 35.0 2.0 35.0 94-95 25.810074157466914 33.0 23.5 35.0 2.0 35.0 96-97 25.23054536111107 32.5 19.0 35.0 2.0 35.0 98-99 24.4525387756072 32.0 6.0 34.0 2.0 35.0 100-101 21.23528695360294 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02895072488720629 1101 2 0.08798413665503801 1101 3 0.041322024753490894 1101 4 0.039137345154472314 1101 5 0.013913224600443641 1101 6 0.11625488982711829 1101 7 0.3470606463151995 1101 8 0.37200961293616075 1101 9 0.5369343196876386 1101 10-11 0.3513912755392923 1101 12-13 0.31633095151266133 1101 14-15 0.4087156457581429 1101 16-17 0.43035404626590434 1101 18-19 0.45376304648706167 1101 20-21 0.31833880841295326 1101 22-23 0.22360446305013681 1101 24-25 0.31969032413982035 1101 26-27 0.3352804661272373 1101 28-29 0.3627212691144237 1101 30-31 0.3598551595800856 1101 32-33 0.41999115791605135 1101 34-35 0.5588423169420835 1101 36-37 0.6912796311764069 1101 38-39 0.6978703226852545 1101 40-41 0.7567260536340328 1101 42-43 0.8055654003120409 1101 44-45 0.7950993476112487 1101 46-47 0.7970761451091946 1101 48-49 0.5388335741258388 1101 50-51 0.5046189185195047 1101 52-53 0.5159429759560297 1101 54-55 0.5574699823657383 1101 56-57 0.3386227339687551 1101 58-59 0.1772685455167604 1101 60-61 0.2817354113231545 1101 62-63 0.2183845098906403 1101 64-65 0.42886507731162027 1101 66-67 0.3660336124248431 1101 68-69 0.0787127962295564 1101 70-71 0.2781708740778228 1101 72-73 0.28091570266682453 1101 74-75 0.07606714691042527 1101 76-77 -0.02257054437921724 1101 78-79 -0.28274147015279993 1101 80-81 -0.19599407945740666 1101 82-83 -0.002840972174823264 1101 84-85 0.28074415765037486 1101 86-87 -0.040007706308088586 1101 88-89 -0.016540826632329697 1101 90-91 -0.1690350327359056 1101 92-93 -0.2388739187278297 1101 94-95 0.21550701100757408 1101 96-97 -0.07194900160261497 1101 98-99 -0.13405416672605242 1101 100-101 0.43150673658477956 1102 1 0.19626145043925547 1102 2 0.04478618712822424 1102 3 0.0787135862676891 1102 4 -0.002404452952781355 1102 5 -0.22900869968977133 1102 6 -0.16313193982587393 1102 7 0.04684869836567174 1102 8 0.03101000725792602 1102 9 0.05013916984536593 1102 10-11 0.017733448252222672 1102 12-13 0.08264355009310265 1102 14-15 0.17591300380230024 1102 16-17 6.985849094363061E-4 1102 18-19 0.12408540453122185 1102 20-21 0.2241082287599525 1102 22-23 0.06755162393341863 1102 24-25 0.03818401499155755 1102 26-27 0.17947900713670606 1102 28-29 -0.05428139255749187 1102 30-31 -0.0047298167606015795 1102 32-33 0.0983478219539009 1102 34-35 0.11890984454460352 1102 36-37 0.0637214687158476 1102 38-39 -0.012147914696448936 1102 40-41 -0.1900676175016116 1102 42-43 -0.1018330618330694 1102 44-45 -0.2559037083824407 1102 46-47 -0.5446258462157232 1102 48-49 -0.4948390402274683 1102 50-51 -0.5664033606603027 1102 52-53 -0.593552785084988 1102 54-55 -0.7959606170033453 1102 56-57 -0.5750996635075936 1102 58-59 -0.561545038868104 1102 60-61 -0.538022573692615 1102 62-63 -0.7452368426330196 1102 64-65 -0.5079793627514704 1102 66-67 -0.372117018489984 1102 68-69 -0.5542870066095595 1102 70-71 -0.5169272134616065 1102 72-73 -0.5339945891312858 1102 74-75 -0.6427148917331102 1102 76-77 -0.5143785096789024 1102 78-79 -0.6778223061149404 1102 80-81 -0.4551669895520476 1102 82-83 -0.3492698956764002 1102 84-85 -0.13059998667139183 1102 86-87 -0.0748189247623472 1102 88-89 -0.036052360544001516 1102 90-91 -0.15466200434473976 1102 92-93 0.03062529262863478 1102 94-95 0.18575296920946727 1102 96-97 0.15348968136268226 1102 98-99 0.18964457144429403 1102 100-101 0.5868300804333586 1103 1 -0.02058631214983464 1103 2 0.18034524776615513 1103 3 0.10416924697571517 1103 4 0.04446141922854707 1103 5 -0.15992936799214874 1103 6 -0.05014171511115251 1103 7 -0.01696327343788795 1103 8 0.0802272055287574 1103 9 0.024337869070350848 1103 10-11 0.12873502553929228 1103 12-13 0.15744784966081937 1103 14-15 0.11081826921493132 1103 16-17 -0.023552203734098498 1103 18-19 -0.27357105845121055 1103 20-21 -0.05564846010556579 1103 22-23 -0.020608499912825096 1103 24-25 0.07709773154722654 1103 26-27 0.03827043526304408 1103 28-29 -0.06550982656458615 1103 30-31 0.057945437357865615 1103 32-33 0.07826662087901326 1103 34-35 0.05275628299146007 1103 36-37 0.31961682253443513 1103 38-39 0.09427271466056197 1103 40-41 -0.022045165501765496 1103 42-43 0.061564628707103 1103 44-45 0.15510127662358997 1103 46-47 0.19756804325734123 1103 48-49 0.027462046348070146 1103 50-51 0.21841135679111545 1103 52-53 0.027353083980720783 1103 54-55 0.10237738977314592 1103 56-57 -0.08745751294483028 1103 58-59 0.02610149304761933 1103 60-61 -0.0593621967015352 1103 62-63 -0.11250862282540908 1103 64-65 -0.10105390416985927 1103 66-67 0.009012007486571605 1103 68-69 0.07988949376042243 1103 70-71 -0.15013738209501426 1103 72-73 0.0888439434075643 1103 74-75 0.10993095863882019 1103 76-77 0.1017735914232496 1103 78-79 0.008886616266956082 1103 80-81 0.19302984029567938 1103 82-83 0.26209922844246236 1103 84-85 0.1874667656750617 1103 86-87 0.18004437702524712 1103 88-89 0.015856935713348008 1103 90-91 0.06761620183199568 1103 92-93 0.2014810195437775 1103 94-95 0.42973347705695986 1103 96-97 0.5349375724714598 1103 98-99 0.4557606480887628 1103 100-101 0.6316610575724297 1104 1 0.11722591868565146 1104 2 0.11535816766279083 1104 3 0.2809780325054305 1104 4 0.241123779262999 1104 5 0.049329891267113624 1104 6 0.11022291308293575 1104 7 0.11829368895085679 1104 8 0.029882674951664967 1104 9 0.16100166464887877 1104 10-11 0.15635130460906055 1104 12-13 0.022090399187085552 1104 14-15 -0.11544859842791055 1104 16-17 0.2709245404519436 1104 18-19 0.3009408565645799 1104 20-21 0.2245948646145095 1104 22-23 0.2143505870811424 1104 24-25 0.3050585411940858 1104 26-27 0.28822378008072747 1104 28-29 0.0620490307423367 1104 30-31 -0.06023931716410402 1104 32-33 0.2085207121796131 1104 34-35 0.17449759794983066 1104 36-37 0.25480918543997433 1104 38-39 0.3591772509798261 1104 40-41 0.4427301718510819 1104 42-43 0.6547780941104833 1104 44-45 0.5382982332701616 1104 46-47 0.7221912129386538 1104 48-49 0.8910925373041394 1104 50-51 0.7480661084419822 1104 52-53 0.754987307351378 1104 54-55 0.83431106763706 1104 56-57 0.5940490905578955 1104 58-59 0.8987256676097815 1104 60-61 1.141201254346413 1104 62-63 1.0534438606658298 1104 64-65 1.0085283525054223 1104 66-67 0.7162758604868635 1104 68-69 0.8966997148342095 1104 70-71 0.7714424341553432 1104 72-73 0.8851187065427943 1104 74-75 0.6188420984608136 1104 76-77 0.6800215098843445 1104 78-79 0.6466916693820863 1104 80-81 0.6628116375968531 1104 82-83 0.7438462855771171 1104 84-85 0.8019226944720756 1104 86-87 1.0546567801260203 1104 88-89 1.2451779233676703 1104 90-91 0.9707420990470403 1104 92-93 0.9766784068535657 1104 94-95 0.6897499858137763 1104 96-97 0.7764642735911842 1104 98-99 1.0877481588553408 1104 100-101 1.3837354187553217 1105 1 0.1332171919530687 1105 2 0.27432395701432455 1105 3 0.12970151577145828 1105 4 0.023531057729321958 1105 5 -0.04488168557919181 1105 6 -0.03851681675971008 1105 7 0.03702134990801653 1105 8 -0.0048803571237172605 1105 9 0.2688673286696712 1105 10-11 0.1163847261380937 1105 12-13 0.19343143803961738 1105 14-15 0.2591928164168209 1105 16-17 0.20137294596649724 1105 18-19 0.1864071333134092 1105 20-21 0.11743592667642844 1105 22-23 0.040034103768697094 1105 24-25 0.1565915217446161 1105 26-27 0.029339223612268484 1105 28-29 0.08468515384496555 1105 30-31 -0.1498473104797995 1105 32-33 -0.013438857054005382 1105 34-35 0.14708614179237856 1105 36-37 -0.037659365829576075 1105 38-39 0.038500000828967984 1105 40-41 -0.058024133491713314 1105 42-43 0.002954996120422493 1105 44-45 -0.03538811496360239 1105 46-47 -0.18074477554948487 1105 48-49 -0.22694580461666902 1105 50-51 -0.26314867130084707 1105 52-53 -0.37009276506194055 1105 54-55 -0.4450000775145 1105 56-57 -0.33293415225879386 1105 58-59 -0.22557108771677292 1105 60-61 -0.3658834135271434 1105 62-63 -0.4701867850195356 1105 64-65 -0.45490176400574356 1105 66-67 -0.23535111811407816 1105 68-69 -0.11564536245307977 1105 70-71 -0.2883008699341545 1105 72-73 -0.4984536835607223 1105 74-75 -0.5245269773410719 1105 76-77 -0.14867458629538888 1105 78-79 -0.3861658713503999 1105 80-81 -0.29059550161309744 1105 82-83 -0.18377753654607787 1105 84-85 -0.1261467480382521 1105 86-87 -0.21766021379311695 1105 88-89 -0.2953909389077758 1105 90-91 -0.03958143992153751 1105 92-93 -0.05552811034459637 1105 94-95 -0.04891570605828832 1105 96-97 0.03159703133151481 1105 98-99 0.16078116261526532 1105 100-101 0.9913121258063349 1106 1 0.12772451698090848 1106 2 0.08131319961258043 1106 3 0.020490216554371443 1106 4 0.10270798937116155 1106 5 -0.1277320901872514 1106 6 0.06785170534688234 1106 7 0.12192109653481964 1106 8 -0.05958354226149254 1106 9 -0.0025948713811771995 1106 10-11 0.07556884691557286 1106 12-13 0.10703030180548723 1106 14-15 0.11934659378156454 1106 16-17 0.15516302320587272 1106 18-19 0.0030973254036084086 1106 20-21 0.013657027666248212 1106 22-23 0.13351478515848214 1106 24-25 0.14873498006954833 1106 26-27 0.0960074976060099 1106 28-29 0.06180046933404526 1106 30-31 0.18435872839414458 1106 32-33 0.23957159349438228 1106 34-35 0.13386405010459868 1106 36-37 -0.001987663845554266 1106 38-39 -0.0171084016924965 1106 40-41 0.19547399470285143 1106 42-43 0.055320616271046674 1106 44-45 0.2860572081529753 1106 46-47 0.06708094928195862 1106 48-49 0.14217247419905021 1106 50-51 0.23185400270691758 1106 52-53 0.060860847478714675 1106 54-55 -0.019341877077884817 1106 56-57 -0.01181650468425488 1106 58-59 0.14055208321808266 1106 60-61 0.09279987874629114 1106 62-63 -0.01313200731286912 1106 64-65 0.03324602533504617 1106 66-67 0.10833778519204884 1106 68-69 -0.028207500256534956 1106 70-71 -0.12801163873330168 1106 72-73 0.3504824120372483 1106 74-75 0.3360563947142303 1106 76-77 0.36436668109662307 1106 78-79 0.5713809676217281 1106 80-81 0.27779115114288544 1106 82-83 0.3302105926128789 1106 84-85 0.4728046902272389 1106 86-87 0.5383289060637999 1106 88-89 0.3519129160470271 1106 90-91 0.16690017956277714 1106 92-93 0.5130569011843207 1106 94-95 0.5801128400705338 1106 96-97 0.643788095761952 1106 98-99 0.8288118654847807 1106 100-101 1.3707636911967853 1107 1 0.1753428531670842 1107 2 0.16325746027020216 1107 3 0.06015402183804497 1107 4 0.1464077533177388 1107 5 0.007736110897823778 1107 6 0.08273630382129227 1107 7 0.10638553698575492 1107 8 0.03646843217814677 1107 9 0.12850228615993586 1107 10-11 0.0017306523614522007 1107 12-13 -0.057026829099577014 1107 14-15 -0.06517739360045738 1107 16-17 -0.03503179557083058 1107 18-19 0.16506497797394104 1107 20-21 0.006419621095169248 1107 22-23 0.06352793243789279 1107 24-25 0.08933318128267587 1107 26-27 0.010886333474182663 1107 28-29 0.30574012844387255 1107 30-31 0.243177863661721 1107 32-33 0.35837945966532203 1107 34-35 0.38930651154849016 1107 36-37 0.19227726237174636 1107 38-39 0.226289145571549 1107 40-41 -0.047868060797455314 1107 42-43 -0.1744227556646365 1107 44-45 -0.07991204087272052 1107 46-47 -0.09689934687331458 1107 48-49 0.06507947427161298 1107 50-51 -0.04098512667000165 1107 52-53 0.14843432070821194 1107 54-55 0.3717192535027678 1107 56-57 0.26252943950810703 1107 58-59 0.3509224267120956 1107 60-61 0.3408758085534771 1107 62-63 0.07675504560492641 1107 64-65 0.014902795970513694 1107 66-67 0.09378507015079052 1107 68-69 0.20645969856191826 1107 70-71 -0.014287671840541805 1107 72-73 0.19394868371638552 1107 74-75 0.2231904158608593 1107 76-77 0.0330872544545997 1107 78-79 0.24255918582387892 1107 80-81 0.5077276771023591 1107 82-83 0.3886199425482104 1107 84-85 0.2793934834521181 1107 86-87 0.1706906355286435 1107 88-89 0.032058389839974666 1107 90-91 0.3938476203253174 1107 92-93 0.3979883261701289 1107 94-95 0.6032006152349823 1107 96-97 0.7077658307588983 1107 98-99 0.8211572035363375 1107 100-101 1.4162370572844871 1108 1 0.1497840582205363 1108 2 0.1890754064963076 1108 3 0.2744073422138129 1108 4 0.07633972610685191 1108 5 0.0921870341242581 1108 6 0.11432036601759421 1108 7 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951.0 10 1504.0 11 2324.0 12 3273.0 13 4321.0 14 5031.0 15 6470.0 16 7822.0 17 10325.0 18 9810.0 19 6495.0 20 5841.0 21 5984.0 22 6289.0 23 6583.0 24 7402.0 25 7999.0 26 8965.0 27 10635.0 28 12724.0 29 15184.0 30 18470.0 31 21462.0 32 26181.0 33 32474.0 34 42718.0 35 59867.0 36 86351.0 37 127443.0 38 101942.0 39 9536.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.88545431809114 20.93736787885645 15.274395145683101 21.902782657369308 2 16.11681026352412 22.46883618967558 38.605102948624456 22.809250598175844 3 18.370050661149538 26.788623742568284 32.236590984365 22.604734611917177 4 12.903666583988937 18.8267584736008 36.81956105552231 31.450013886887955 5 12.492497578426304 38.44968772098717 34.444583103060985 14.61323159752554 6 29.013878656812185 37.47697782741986 17.902784049050595 15.606359466717365 7 24.97560511928614 35.39849427518614 21.862862897057315 17.763037708470407 8 25.2782947198636 34.402945505475294 21.144012226402314 19.174747548258793 9 25.72980423201054 16.724417307666602 21.74656949310631 35.79920896721655 10-11 23.713235021097674 27.47056648106259 27.888064256932676 20.92813424090706 12-13 25.134598847165517 25.665642353968604 28.338756893317512 20.861001905548367 14-15 22.336205270482417 26.932765183863882 27.548243939895766 23.182785605757935 16-17 20.360980159755705 29.829357264012785 28.474790502585854 21.334872073645656 18-19 20.159305625897638 27.476952964293616 31.860456826630745 20.503284583178 20-21 21.22161542380257 26.754909052023105 31.936426272506786 20.08704925166754 22-23 21.08824189028022 26.976059599255603 31.639603325129922 20.29609518533426 24-25 20.772841028078332 27.257220089262507 31.957982756444054 20.011956126215104 26-27 20.494869951640307 27.276663181283496 31.99514917836052 20.233317688715676 28-29 20.204679361411085 27.395265388082823 31.993978883018936 20.406076367487156 30-31 20.613785595361335 27.33697016324148 31.73072210332142 20.318522138075767 32-33 20.421968314131803 27.403582668569737 31.70190866734888 20.47254034994958 34-35 20.548247356683095 27.46554237661984 31.740968682719533 20.24524158397753 36-37 20.940814013425772 27.202707139069172 31.347791016707195 20.508687830797868 38-39 20.357526945609678 27.322462002828253 31.522426946798017 20.797584104764056 40-41 20.487535341967654 27.2466054258229 31.631051766559942 20.6348074656495 42-43 20.45932553828604 27.39881886021199 31.147808831218214 20.99404677028376 44-45 20.400283688996655 27.571674173533005 30.988195715702037 21.039846421768303 46-47 20.88765284033314 27.64912723667648 30.122272266511214 21.340947656479166 48-49 20.708809884691963 27.79356690012454 29.908055076353424 21.589568138830074 50-51 20.55445071045913 28.12584936513901 29.720019545609144 21.59968037879272 52-53 20.613785595361335 28.228033096835425 29.694028044087528 21.464153263715716 54-55 20.461252612145902 28.11144264715274 29.47458550981216 21.95271923088919 56-57 20.505561863712618 28.50277851833644 28.85752880814448 22.134130809806464 58-59 20.253648668819764 28.225654933546814 29.420082029605215 22.100614368028207 60-61 20.443203422053234 28.45094165874525 29.18570282794677 21.920152091254753 62-63 20.152326182399317 28.51979527456141 29.224838554445913 22.10303998859336 64-65 19.953585777196878 28.50739285740808 29.104094105765014 22.434927259630022 66-67 20.055743819053156 28.453409797276976 28.706358701746804 22.784487681923064 68-69 20.24574593381524 28.22899849546337 28.722762762941166 22.802492807780222 70-71 20.275703405337325 27.92271155628431 28.59217936836102 23.209405670017347 72-73 20.223838811104162 27.781276316395292 28.471090766779 23.523794105721546 74-75 20.120467343784927 28.20073533943913 28.034686158879957 23.64411115789599 76-77 20.100875902429166 27.716649873830818 28.527684401684635 23.654789822055385 78-79 20.18294999996282 27.629153315252996 28.32571971215232 23.86217697263186 80-81 20.231178244144424 27.602073400763416 28.125621945966824 24.04112640912534 82-83 20.27671500626771 27.37430416520618 28.057365897708575 24.291614930817534 84-85 20.14200271202753 27.159856645729892 28.029235240911515 24.668905401331063 86-87 20.207511570959383 27.049951753596922 27.90887153967728 24.83366513576642 88-89 20.313049569286715 26.731795284509225 27.796134012936303 25.159021133267757 90-91 20.181980284226785 26.599521120208962 27.884162377820054 25.334336217744198 92-93 20.340979301822674 26.42574736817091 27.84198925881039 25.391284071196026 94-95 20.15789524494853 26.2023527594537 27.956785626497766 25.6829663691 96-97 20.254805828631348 25.908848546778017 27.900985746260567 25.93535987833007 98-99 20.134131000138126 25.889243528487427 27.753873847648215 26.222751623726232 100-101 20.28475036444096 25.412544195196286 28.30133753359429 26.001367906768465 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1680.0 1 1104.5 2 590.5 3 653.0 4 589.5 5 597.5 6 898.5 7 1476.0 8 2042.5 9 2196.5 10 1850.5 11 1531.0 12 1519.5 13 1567.0 14 1590.0 15 1652.5 16 1754.0 17 1816.5 18 1922.0 19 2072.5 20 2209.0 21 2501.5 22 2833.5 23 3289.5 24 3749.5 25 4309.0 26 5271.0 27 6413.5 28 7314.0 29 8148.5 30 9154.0 31 10446.0 32 12220.5 33 13371.0 34 14175.0 35 15218.5 36 16635.0 37 17850.0 38 18525.5 39 18937.5 40 19230.5 41 19791.5 42 19926.5 43 20650.5 44 21760.5 45 22592.5 46 22958.5 47 23312.0 48 24752.0 49 24734.0 50 23253.5 51 22484.0 52 22096.0 53 21706.0 54 20718.0 55 18935.0 56 17177.0 57 15307.5 58 13921.0 59 12612.5 60 10504.0 61 8557.5 62 6864.5 63 4993.5 64 3451.5 65 2513.0 66 1860.5 67 1451.0 68 1093.0 69 801.5 70 598.5 71 425.0 72 341.0 73 311.0 74 235.0 75 181.0 76 133.5 77 76.5 78 45.0 79 32.5 80 23.5 81 13.0 82 10.5 83 9.0 84 5.0 85 3.0 86 3.5 87 2.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23243828503617275 2 0.0 3 0.0 4 0.0 5 0.026882638716643623 6 0.004901254572647732 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002821934450918391 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.970457316756201E-4 26-27 0.0013367057925402907 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0063122217981069284 36-37 0.016931606705510346 38-39 0.013218535059565094 40-41 0.007945973322322839 42-43 0.007277620426052693 44-45 0.0036388102130263467 46-47 6.683528962701453E-4 48-49 0.002005058688810436 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.653986279457655E-4 58-59 5.198300304323352E-4 60-61 0.002524888719242771 62-63 7.426143291890503E-4 64-65 0.0017822743900537208 66-67 0.005718130334755687 68-69 0.0 70-71 1.4852286583781006E-4 72-73 0.0 74-75 3.7130716459452516E-4 76-77 0.05807244054258373 78-79 0.13775495806456883 80-81 0.0010396600608646705 82-83 7.426143291890503E-4 84-85 0.0 86-87 0.10515418901316952 88-89 0.3441274801462059 90-91 0.3809611508739828 92-93 1.4852286583781006E-4 94-95 3.7130716459452516E-4 96-97 0.0 98-99 0.0 100-101 8.168757621079554E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 673297.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.33937300809879 #Duplication Level Percentage of deduplicated Percentage of total 1 84.01028748810498 54.05169223214801 2 9.619232846341387 12.377908203050284 3 2.5957786563124063 5.010323136448362 4 1.1343094595293373 2.9192303769309187 5 0.641944856968467 2.065116480156241 6 0.38611704834855803 1.4905517279090392 7 0.2878086329630059 1.2962198893810482 8 0.1966842122750024 1.0123631118692369 9 0.15221766881480486 0.8814230435069079 >10 0.8949232023143974 10.439178941970363 >50 0.05464277236907703 2.37226660703429 >100 0.023055889963503353 2.5105087059905005 >500 0.0011527944981161169 0.5156303629014712 >1k 0.0016139122973625635 2.3034258559073777 >5k 2.305588996232234E-4 0.7541613247960529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5084 0.7550902499194263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4436 0.6588474328565255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2998 0.44527155178175454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2467 0.3664059100218774 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2148 0.319027115819616 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.1875843795531541 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1145 0.1700586813842925 TruSeq Adapter, Index 10 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.15906798931229457 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.1268385274254898 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 746 0.11079805791500631 TruSeq Adapter, Index 10 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.4852286583781006E-4 0.0 6 0.0 0.0 0.0 1.4852286583781006E-4 0.0 7 0.0 0.0 0.0 1.4852286583781006E-4 0.0 8 0.0 0.0 0.0 1.4852286583781006E-4 0.0 9 0.0 0.0 0.0 1.4852286583781006E-4 0.0 10-11 0.0 0.0 0.0 1.4852286583781006E-4 0.0 12-13 0.0 0.0 0.0 1.4852286583781006E-4 1.4852286583781006E-4 14-15 0.0 0.0 0.0 1.4852286583781006E-4 1.4852286583781006E-4 16-17 0.0 0.0 0.0 1.4852286583781006E-4 1.4852286583781006E-4 18-19 0.0 0.0 0.0 1.4852286583781006E-4 1.4852286583781006E-4 20-21 0.0 0.0 0.0 3.7130716459452516E-4 1.4852286583781006E-4 22-23 0.0 0.0 0.0 0.0015594900912970055 1.4852286583781006E-4 24-25 0.0 0.0 0.0 0.005124038871404447 1.4852286583781006E-4 26-27 0.0 0.0 0.0 0.01047086204156561 1.4852286583781006E-4 28-29 0.0 0.0 0.0 0.027253945881238147 1.4852286583781006E-4 30-31 0.0 1.4852286583781006E-4 0.0 0.08332132773501144 1.4852286583781006E-4 32-33 0.0 2.2278429875671507E-4 0.0 0.17852448473704768 1.4852286583781006E-4 34-35 0.0 2.970457316756201E-4 0.0 0.33477053959842384 1.4852286583781006E-4 36-37 0.0 2.970457316756201E-4 0.0 0.5595598970439494 1.4852286583781006E-4 38-39 0.0 2.970457316756201E-4 0.0 0.871012346705837 1.4852286583781006E-4 40-41 0.0 2.970457316756201E-4 0.0 1.1993964030732351 1.4852286583781006E-4 42-43 0.0 2.970457316756201E-4 0.0 1.5182007345940944 2.2278429875671507E-4 44-45 0.0 2.970457316756201E-4 0.0 1.909409963210886 2.970457316756201E-4 46-47 0.0 2.970457316756201E-4 0.0 2.3698308473080973 2.970457316756201E-4 48-49 0.0 2.970457316756201E-4 0.0 2.8267614440581204 2.970457316756201E-4 50-51 7.426143291890503E-5 2.970457316756201E-4 0.0 3.3069358693117596 4.455685975134302E-4 52-53 1.4852286583781006E-4 2.970457316756201E-4 0.0 3.779832674139347 4.455685975134302E-4 54-55 1.4852286583781006E-4 2.970457316756201E-4 0.0 4.3043411748455735 5.940914633512402E-4 56-57 1.4852286583781006E-4 2.970457316756201E-4 0.0 4.89390269078876 5.940914633512402E-4 58-59 1.4852286583781006E-4 2.970457316756201E-4 0.0 5.516659067246698 5.940914633512402E-4 60-61 1.4852286583781006E-4 2.970457316756201E-4 0.0 6.18389804202306 5.940914633512402E-4 62-63 1.4852286583781006E-4 2.970457316756201E-4 0.0 6.838067004605694 5.940914633512402E-4 64-65 1.4852286583781006E-4 2.970457316756201E-4 0.0 7.537981009866374 5.940914633512402E-4 66-67 1.4852286583781006E-4 2.970457316756201E-4 0.0 8.301685586004393 5.940914633512402E-4 68-69 1.4852286583781006E-4 2.970457316756201E-4 0.0 9.133265111830292 5.940914633512402E-4 70-71 1.4852286583781006E-4 2.970457316756201E-4 0.0 10.028486685667692 5.940914633512402E-4 72-73 1.4852286583781006E-4 2.970457316756201E-4 0.0 10.918212913469093 5.940914633512402E-4 74-75 1.4852286583781006E-4 2.970457316756201E-4 0.0 11.82784120529276 5.940914633512402E-4 76-77 1.4852286583781006E-4 2.970457316756201E-4 0.0 12.772446632021232 5.940914633512402E-4 78-79 1.4852286583781006E-4 2.970457316756201E-4 0.0 13.795694916210824 5.940914633512402E-4 80-81 1.4852286583781006E-4 2.970457316756201E-4 0.0 14.86706460893187 5.940914633512402E-4 82-83 1.4852286583781006E-4 2.970457316756201E-4 0.0 15.956851137016798 5.940914633512402E-4 84-85 1.4852286583781006E-4 2.970457316756201E-4 0.0 17.043518684919135 5.940914633512402E-4 86-87 1.4852286583781006E-4 2.970457316756201E-4 0.0 18.174000478243627 5.940914633512402E-4 88-89 1.4852286583781006E-4 2.970457316756201E-4 0.0 19.3381969621133 5.940914633512402E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3160 0.0 43.210293 1 GTATCAA 4925 0.0 32.941284 1 TATCAAC 6200 0.0 26.0225 2 ATCAACG 6245 0.0 25.759 3 TCAACGC 6285 0.0 25.595062 4 CAACGCA 6285 0.0 25.51956 5 AACGCAG 6420 0.0 24.90347 6 GATATAG 165 2.3936827E-6 23.067389 1 GCATATA 125 2.2301183E-4 22.836714 1 CTATACC 105 0.0022080045 22.59657 4 GTACATG 3530 0.0 22.37308 1 ACGCAGA 7240 0.0 21.8208 7 ACGCAGT 110 0.002896525 21.564646 8 CGCAGAG 7265 0.0 21.549805 8 TACATGG 3560 0.0 21.460392 2 GAGTACT 4550 0.0 21.376654 12-13 ACATGGG 3440 0.0 20.829569 3 AGTACTT 4675 0.0 20.50062 12-13 GCAGAGT 7620 0.0 20.172281 9 GTACTTT 4910 0.0 19.809324 14-15 >>END_MODULE