FastQCFastQC Report
Thu 26 May 2016
SRR938837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95789
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.8017622065164058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.6931902410506426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.4489033187526752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.16703379302425123No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1534622973410308No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1390.14511060768981823No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.14302268527701512No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.14093476286421197No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.111703849084968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACC250.005066795756.8300069
GGACACT352.8162464E-454.1238176
ATGGACA405.428825E-447.3831064
GTATCAA10450.028.580841
GGTATCA7350.028.380241
TGATCCA350.00832528327.17555258-59
TGCACAA909.0162124E-426.3239465
TATCAAC12500.023.8810842
CAACGCA12850.023.599375
TCAACGC12850.023.599374
ATCAACG12700.023.5050073
AACGCAG12750.023.400596
TTAATCC1050.002213095322.5633834
AGAGTAC10150.021.46289310-11
CGCAGAG14150.020.750658
ACGCAGA14300.020.2018097
GCAGAGT14450.019.9921029
GAGTACA5100.019.52091
AGTACTT6900.019.21787512-13
GTACATG5250.018.9631611
Produced by FastQC (version 0.11.5)