##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938837_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95789 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.34542588397415 31.0 31.0 34.0 26.0 34.0 2 30.364238064913508 33.0 31.0 34.0 26.0 34.0 3 30.323669732432744 33.0 31.0 34.0 26.0 34.0 4 33.77807472674316 37.0 35.0 37.0 28.0 37.0 5 33.75048283205796 37.0 35.0 37.0 28.0 37.0 6 33.84418878994457 37.0 35.0 37.0 30.0 37.0 7 33.75172514589358 37.0 35.0 37.0 29.0 37.0 8 33.72388270051885 37.0 35.0 37.0 29.0 37.0 9 35.17838165133784 39.0 35.0 39.0 28.0 39.0 10-11 35.05195272943658 39.0 35.0 39.0 28.0 39.0 12-13 34.9221465930326 39.0 35.0 39.0 27.0 39.0 14-15 35.92933426593868 40.0 36.0 41.0 27.0 41.0 16-17 35.865809226529144 40.0 36.0 41.0 27.0 41.0 18-19 35.872469699025984 40.0 36.0 41.0 27.0 41.0 20-21 35.78099781812108 40.0 36.0 41.0 27.0 41.0 22-23 35.61619810207853 40.0 36.0 41.0 26.5 41.0 24-25 35.52560836839304 40.0 36.0 41.0 25.5 41.0 26-27 35.36164904112163 39.0 35.0 41.0 25.0 41.0 28-29 35.12413742705321 39.0 35.0 41.0 24.0 41.0 30-31 34.774765369718864 39.0 35.0 41.0 23.0 41.0 32-33 34.389032143565544 38.5 35.0 41.0 20.0 41.0 34-35 33.95452504984915 38.0 34.0 41.0 15.5 41.0 36-37 33.58884109866477 38.0 34.0 40.0 9.0 41.0 38-39 33.28317447723643 38.0 33.5 40.0 5.0 41.0 40-41 32.95949430519162 38.0 33.0 40.0 2.0 41.0 42-43 32.523645721324996 38.0 33.0 40.0 2.0 41.0 44-45 32.144525989414234 38.0 32.5 40.0 2.0 41.0 46-47 31.677102798859995 38.0 31.0 40.0 2.0 41.0 48-49 31.238941840921193 38.0 31.0 40.0 2.0 41.0 50-51 30.104307383937616 36.5 28.5 39.0 2.0 40.5 52-53 30.1222113186274 37.0 28.5 39.5 2.0 40.5 54-55 30.488907912181986 37.0 29.0 40.0 2.0 41.0 56-57 30.339370908977024 37.0 29.0 40.0 2.0 41.0 58-59 30.087703180949795 37.0 28.0 40.0 2.0 41.0 60-61 29.77457745670171 36.5 28.0 40.0 2.0 41.0 62-63 29.39611542035098 36.0 26.5 40.0 2.0 41.0 64-65 29.014991282923926 35.0 26.0 39.0 2.0 41.0 66-67 28.585818830972244 35.0 25.5 39.0 2.0 41.0 68-69 28.15270020566036 35.0 23.5 38.5 2.0 40.0 70-71 27.728700581486393 35.0 22.5 37.5 2.0 40.0 72-73 27.297027842445374 35.0 21.5 37.0 2.0 39.5 74-75 26.81586612241489 34.0 19.0 36.5 2.0 39.0 76-77 26.359936944743133 34.0 15.0 36.0 2.0 39.0 78-79 25.92161939262337 34.0 8.5 35.5 2.0 37.5 80-81 25.47467872093873 34.0 3.5 35.0 2.0 37.0 82-83 25.026114689578137 33.5 2.0 35.0 2.0 37.0 84-85 24.605168651932892 33.0 2.0 35.0 2.0 36.0 86-87 24.189734729457452 33.0 2.0 35.0 2.0 36.0 88-89 23.794344862144925 33.0 2.0 35.0 2.0 35.5 90-91 23.368680119846744 32.0 2.0 35.0 2.0 35.0 92-93 22.920726805791897 32.0 2.0 35.0 2.0 35.0 94-95 22.44785935754627 31.5 2.0 35.0 2.0 35.0 96-97 21.88925137541889 31.0 2.0 35.0 2.0 35.0 98-99 21.26747852049818 31.0 2.0 35.0 2.0 35.0 100-101 19.56394784369813 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.1368374438339437 1101 2 -0.8111231798482237 1101 3 -0.6925234257459998 1101 4 -1.083470713557439 1101 5 -0.6937952482136751 1101 6 -1.5350054656346899 1101 7 -1.1046120662405556 1101 8 -1.1907816731976837 1101 9 -0.9013634398551673 1101 10-11 -0.9654741220305141 1101 12-13 -0.8608727073820646 1101 14-15 -0.6405687952085586 1101 16-17 -0.5650143559860936 1101 18-19 -0.41477198691730877 1101 20-21 -0.9667118832571688 1101 22-23 -0.9632765826245944 1101 24-25 -1.1347996776072904 1101 26-27 -0.6348640591780637 1101 28-29 -1.0913926505379976 1101 30-31 -0.7297734973871073 1101 32-33 -0.42524664208514906 1101 34-35 -0.33958519634244766 1101 36-37 -0.634748971161919 1101 38-39 -0.44659387656516003 1101 40-41 0.3684355665923178 1101 42-43 -0.09274549562421441 1101 44-45 0.21264288248268315 1101 46-47 1.392017134466002 1101 48-49 1.4137385389035337 1101 50-51 1.7287837895130487 1101 52-53 1.4651953270309512 1101 54-55 1.7671456236151606 1101 56-57 2.101081535534618 1101 58-59 2.085702816066373 1101 60-61 1.9360533018886557 1101 62-63 1.9631266763758433 1101 64-65 1.9767682688678363 1101 66-67 1.4368117754848448 1101 68-69 1.1180530291140514 1101 70-71 0.8632385949208405 1101 72-73 0.8878246508651415 1101 74-75 0.9680148680582619 1101 76-77 0.8319014767660562 1101 78-79 0.5058462004382207 1101 80-81 0.59635230657695 1101 82-83 0.5988555164208016 1101 84-85 0.6281862082214538 1101 86-87 0.893013482070117 1101 88-89 1.5644566483285907 1101 90-91 1.5625286353610974 1101 92-93 1.4558345746979278 1101 94-95 1.1341175064280797 1101 96-97 1.081670787720146 1101 98-99 0.06263851670661325 1101 100-101 -0.15954609443370416 1102 1 0.40421345928592345 1102 2 0.5345254244210693 1102 3 0.3173287910027689 1102 4 0.7851663964589832 1102 5 -0.008655675143067754 1102 6 -0.23656539995324266 1102 7 -0.3262869430878439 1102 8 -0.29299842196615344 1102 9 -0.7297706648141116 1102 10-11 -0.45582715979734445 1102 12-13 -0.642071393753163 1102 14-15 -0.913967809986886 1102 16-17 -0.9605398075460272 1102 18-19 -0.6781135304312613 1102 20-21 -0.644051784734998 1102 22-23 -0.3415606548741863 1102 24-25 0.17061904160453167 1102 26-27 -0.8754223514604931 1102 28-29 -0.9183630938877485 1102 30-31 -0.4596585548583718 1102 32-33 -0.4281202053035358 1102 34-35 -0.2437313375575485 1102 36-37 -0.5452580023606046 1102 38-39 -0.048605370818030735 1102 40-41 0.21059484409642693 1102 42-43 0.12728734509828143 1102 44-45 0.8706888594941802 1102 46-47 0.5192749341375986 1102 48-49 0.7546662893140414 1102 50-51 0.5469282886920332 1102 52-53 1.0115828475564008 1102 54-55 0.8584838830568629 1102 56-57 0.8755068228909337 1102 58-59 0.8291346715671999 1102 60-61 1.1037462411333188 1102 62-63 0.6209673988717377 1102 64-65 0.11326252174140095 1102 66-67 0.41731259650290653 1102 68-69 1.153972569343935 1102 70-71 1.3583124865464526 1102 72-73 1.4192285917518426 1102 74-75 0.6958473803735323 1102 76-77 0.18473398908132665 1102 78-79 0.06454899189963115 1102 80-81 0.08054770887580176 1102 82-83 0.09146635385922508 1102 84-85 -0.14685484268166604 1102 86-87 -0.11745449822544884 1102 88-89 -0.6861591152182065 1102 90-91 -0.7123071610264233 1102 92-93 -0.9335332413940272 1102 94-95 0.20451980527865388 1102 96-97 -0.7221058953668802 1102 98-99 -1.3731168199107877 1102 100-101 -1.4136511847456923 1103 1 0.7809114739149727 1103 2 0.9437469500219073 1103 3 0.752281769059195 1103 4 1.0729145678278442 1103 5 1.2888887344703122 1103 6 1.1424837118544886 1103 7 1.5880286264001384 1103 8 1.2350841276681166 1103 9 1.6960391575474318 1103 10-11 0.9847423281859378 1103 12-13 1.2059562969469368 1103 14-15 1.947905230765464 1103 16-17 1.2658839124121712 1103 18-19 1.3460505239051983 1103 20-21 1.8639158223705365 1103 22-23 1.2161065342585218 1103 24-25 0.9685553007476884 1103 26-27 1.1578848585708528 1103 28-29 1.6688887347433905 1103 30-31 1.5410598359462213 1103 32-33 1.8891472973087957 1103 34-35 2.3379039811467237 1103 36-37 2.6517770028640584 1103 38-39 2.1234818810105978 1103 40-41 2.457721280878033 1103 42-43 2.2730553701766496 1103 44-45 2.5743853067251123 1103 46-47 2.936659991608863 1103 48-49 2.1794311795961754 1103 50-51 2.413307599036859 1103 52-53 2.310433422269046 1103 54-55 2.484136965606492 1103 56-57 2.496914868867947 1103 58-59 2.7437114740750417 1103 60-61 3.3256636914990416 1103 62-63 3.313234901484069 1103 64-65 2.531692511292082 1103 66-67 2.9565628577359284 1103 68-69 2.6218409079019303 1103 70-71 2.6010632702455148 1103 72-73 2.5182359062763986 1103 74-75 2.410384997928393 1103 76-77 1.5526806975452772 1103 78-79 1.7801968497888687 1103 80-81 1.6986250338496767 1103 82-83 1.3732061657714496 1103 84-85 0.8243442168794637 1103 86-87 -0.08480037074373925 1103 88-89 -0.15415807028612605 1103 90-91 -0.29407309624063416 1103 92-93 -0.2581805768172245 1103 94-95 0.3670720518826265 1103 96-97 0.5088894024387649 1103 98-99 0.30452163358973294 1103 100-101 1.0793608319732222 1104 1 0.30089149389499426 1104 2 1.106459237734196 1104 3 0.5162677830452083 1104 4 1.3048076747209478 1104 5 0.8895380851196606 1104 6 0.603272922643697 1104 7 0.9027139410854517 1104 8 0.7387055062894987 1104 9 1.1151200766283509 1104 10-11 0.6769251453687559 1104 12-13 1.193156596647242 1104 14-15 1.1231674685277042 1104 16-17 0.7010112850395487 1104 18-19 0.788982591837275 1104 20-21 0.9636910471457654 1104 22-23 0.7056794613314494 1104 24-25 0.6252735824659723 1104 26-27 0.9182494939354839 1104 28-29 0.3861714520261117 1104 30-31 0.6200433524297395 1104 32-33 -0.32176678860529506 1104 34-35 -0.021361753119002458 1104 36-37 0.45865762224467943 1104 38-39 1.2049628999916138 1104 40-41 1.3093696325263835 1104 42-43 0.4383900355113184 1104 44-45 0.6366355564753619 1104 46-47 0.1997094421583121 1104 48-49 -0.49789149772650276 1104 50-51 -0.6347693240400645 1104 52-53 -0.8324237205880998 1104 54-55 -1.106022874553343 1104 56-57 -1.715310405857327 1104 58-59 -1.7870078066442439 1104 60-61 -0.894990654155297 1104 62-63 -0.8331187448695765 1104 64-65 -0.6967665296669594 1104 66-67 -0.8099830963100239 1104 68-69 -0.7065806705196493 1104 70-71 -0.40599217430993306 1104 72-73 -0.42307278503229284 1104 74-75 -0.22154557150217613 1104 76-77 -1.0843073144427358 1104 78-79 -1.3979999534079326 1104 80-81 -1.3967795615549186 1104 82-83 -1.467078549513264 1104 84-85 -1.3241947441595023 1104 86-87 -0.919715455658821 1104 88-89 -0.7381990293270881 1104 90-91 -0.578039130206669 1104 92-93 -0.45304820918486044 1104 94-95 -0.2216517243411502 1104 96-97 0.18240338845274806 1104 98-99 1.242583571651668 1104 100-101 0.9054722205846097 1105 1 -0.1420134272707969 1105 2 0.0025414164250676663 1105 3 -0.21115696611867207 1105 4 -0.7625597404725539 1105 5 -0.9117041509051873 1105 6 -0.728069647829308 1105 7 -0.3773863933627055 1105 8 -0.72390796719354 1105 9 -0.31285412308497484 1105 10-11 -0.28353830422512516 1105 12-13 -0.3471563512744069 1105 14-15 -0.4930532672582544 1105 16-17 -0.5678611468763606 1105 18-19 -0.8288506618655447 1105 20-21 -0.8070327942302526 1105 22-23 -0.022282793804720313 1105 24-25 -0.04033797988471832 1105 26-27 0.01756865303725874 1105 28-29 0.2706673908698747 1105 30-31 0.25573374898970513 1105 32-33 0.360985241972827 1105 34-35 0.8423506214629342 1105 36-37 0.8373007182790744 1105 38-39 0.013551050971067014 1105 40-41 0.49032997653020516 1105 42-43 0.8950909432991807 1105 44-45 1.1438023027725421 1105 46-47 1.4122034698697377 1105 48-49 1.1828896776198938 1105 50-51 0.7230902077325112 1105 52-53 0.9789116831386124 1105 54-55 1.5626942778594604 1105 56-57 1.0376757384331654 1105 58-59 0.8499367705177221 1105 60-61 0.545525351578096 1105 62-63 0.5894206722350575 1105 64-65 1.1198842108968243 1105 66-67 0.5561181937043074 1105 68-69 0.6959515798386882 1105 70-71 0.7149466694549993 1105 72-73 0.6373070525214573 1105 74-75 0.41754902954894746 1105 76-77 0.7162182469523906 1105 78-79 0.6230822252829427 1105 80-81 0.900700132663907 1105 82-83 1.0856567586568282 1105 84-85 1.590401529132425 1105 86-87 1.2343695897305729 1105 88-89 0.3744980561960851 1105 90-91 0.6412035836012606 1105 92-93 0.5627186326689397 1105 94-95 0.9547696803411228 1105 96-97 0.7956977028340191 1105 98-99 1.427545349004749 1105 100-101 1.683362808257808 1106 1 -0.6992348165761442 1106 2 -0.40800331121111455 1106 3 -0.2803723584225182 1106 4 -0.19184509779389458 1106 5 -0.847736134913184 1106 6 -0.3744964344360042 1106 7 -0.337781195961405 1106 8 -0.6723087667937406 1106 9 -0.2929890556187118 1106 10-11 -0.11099957359044765 1106 12-13 -1.0788530029485628 1106 14-15 -0.4312091892972276 1106 16-17 -0.8685857845575171 1106 18-19 -0.9137457143393064 1106 20-21 -0.5578448881832756 1106 22-23 -0.582939965219019 1106 24-25 -0.3983464756368491 1106 26-27 -0.5363418916903768 1106 28-29 -0.14250102492222538 1106 30-31 -0.2182792445135462 1106 32-33 -1.5085799754184777 1106 34-35 -1.1632715674426066 1106 36-37 -1.3670970828203721 1106 38-39 -2.1060766351858504 1106 40-41 -2.030784466248406 1106 42-43 -1.4531724250166569 1106 44-45 -1.324713439356394 1106 46-47 -1.308311272758953 1106 48-49 -1.7395865842491745 1106 50-51 -1.2749107917677378 1106 52-53 -1.6378424397999218 1106 54-55 -1.7564586456787694 1106 56-57 -1.3716196138906724 1106 58-59 -1.7800607307316518 1106 60-61 -1.2468284715103586 1106 62-63 -1.8388027160707914 1106 64-65 -2.3982317311321637 1106 66-67 -2.076940277060313 1106 68-69 -1.7540734076675584 1106 70-71 -1.6244461341432022 1106 72-73 -1.5980127875585026 1106 74-75 -2.125991700086235 1106 76-77 -1.4359556660910862 1106 78-79 -2.1078648012038173 1106 80-81 -2.0343948198598305 1106 82-83 -2.0752003128074428 1106 84-85 -1.8939812794632758 1106 86-87 -1.5255004752369423 1106 88-89 -1.875814287632 1106 90-91 -2.195065781716078 1106 92-93 -1.36456772415265 1106 94-95 -1.0943307694339879 1106 96-97 -1.4175081942174543 1106 98-99 -1.9535765900257331 1106 100-101 -1.7642258973893696 1107 1 -0.21810038379253527 1107 2 -0.2800672792271044 1107 3 -0.24376566177084413 1107 4 0.33526634648396936 1107 5 -0.1617041509051873 1107 6 -0.05415660435104286 1107 7 -0.18173421944966606 1107 8 -0.017386228063109854 1107 9 -0.9758976013458494 1107 10-11 -0.8541904781381717 1107 12-13 -0.5101998295352743 1107 14-15 -0.6669663107365125 1107 16-17 -0.15481766861549318 1107 18-19 -0.16037240099598193 1107 20-21 -0.453771924665034 1107 22-23 -0.18532627206558772 1107 24-25 -0.7088162407542811 1107 26-27 -0.6454748252236087 1107 28-29 -1.2619413047822974 1107 30-31 -0.9073097292711694 1107 32-33 -0.5248843232445637 1107 34-35 -0.896779813319668 1107 36-37 -1.0159601512861443 1107 38-39 -0.40492720989849573 1107 40-41 -0.7433656756437017 1107 42-43 -1.1918655784399448 1107 44-45 -0.6061976972274579 1107 46-47 -0.9193182692607031 1107 48-49 -0.34971901803228533 1107 50-51 -0.05951848791966441 1107 52-53 0.04956385705165545 1107 54-55 0.486607321337722 1107 56-57 -0.03297643547988116 1107 58-59 -0.2370197512214034 1107 60-61 -0.3403442136392947 1107 62-63 -0.15514454515625076 1107 64-65 -0.4127244847553513 1107 66-67 -0.42757745846960304 1107 68-69 -0.39100494190044444 1107 70-71 -0.30135767837108673 1107 72-73 -0.48769294747854275 1107 74-75 -0.1639727095814898 1107 76-77 0.2270878121697848 1107 78-79 0.37308222528294266 1107 80-81 0.205047958750864 1107 82-83 0.7269611064829142 1107 84-85 0.1610537030454715 1107 86-87 1.071326111469702 1107 88-89 0.7603676214134758 1107 90-91 1.5542470618621351 1107 92-93 1.426849067451549 1107 94-95 2.166726202080252 1107 96-97 1.1924368332688005 1107 98-99 1.3460236098743188 1107 100-101 1.1018410691273708 1108 1 0.31153348348435017 1108 2 0.15654599308410866 1108 3 -0.29101966634750553 1108 4 0.06490021376085764 1108 5 0.19630500241289184 1108 6 0.2039669654430014 1108 7 0.34400948764415773 1108 8 -0.1480498436237454 1108 9 -0.563769454892757 1108 10-11 0.14060357220507314 1108 12-13 0.5633127562770852 1108 14-15 0.8308597762200094 1108 16-17 0.7121159698284387 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2312 20-21 0.391880249248004 2312 22-23 0.5592389453257098 2312 24-25 -0.0022945016238509197 2312 26-27 0.29474256608073546 2312 28-29 0.5119717386959621 2312 30-31 0.5774728794244837 2312 32-33 1.0870721984945604 2312 34-35 0.6401767084194603 2312 36-37 0.8025181095834242 2312 38-39 0.87333365966672 2312 40-41 1.2925038895736805 2312 42-43 1.1244387693861455 2312 44-45 1.969889259294284 2312 46-47 2.1926382524784316 2312 48-49 2.403541851532932 2312 50-51 1.7709162946890338 2312 52-53 1.627824726616872 2312 54-55 2.1942160169898948 2312 56-57 2.250719216694037 2312 58-59 1.9988498139959887 2312 60-61 2.032481873317227 2312 62-63 1.557898933104621 2312 64-65 2.104666819592474 2312 66-67 1.7495964545738723 2312 68-69 1.4959515798386889 2312 70-71 1.6377727564115219 2312 72-73 2.2623070525214573 2312 74-75 1.9621142469402493 2312 76-77 1.4901312904306536 2312 78-79 0.8502561383264187 2312 80-81 1.6930914370117343 2312 82-83 1.3106567586568225 2312 84-85 1.253445007393296 2312 86-87 0.44741306799144454 2312 88-89 1.1342806648917367 2312 90-91 0.6401166270795215 2312 92-93 0.5714142848428523 2312 94-95 0.26563924555851415 2312 96-97 -0.603215340644244 2312 98-99 -0.1800633466474224 2312 100-101 -0.176419800437845 2313 1 -0.1578997148962138 2313 2 0.32486583114078726 2313 3 -0.3106552938778684 2313 4 0.04931316922644413 2313 5 0.271406216987792 2313 6 0.40821797758875533 2313 7 0.04694471031622527 2313 8 0.2744197920037834 2313 9 0.7030321645404385 2313 10-11 -0.21936606342245568 2313 12-13 -0.26082691983627626 2313 14-15 -0.18933253147229578 2313 16-17 -0.17948322045496212 2313 18-19 -0.46994597959130147 2313 20-21 -0.9429023594476433 2313 22-23 -0.8053095496575651 2313 24-25 -0.1254132307208451 2313 26-27 -0.872272484086487 2313 28-29 -0.8123175589629028 2313 30-31 -0.3531709332845523 2313 32-33 -0.36434920618771116 2313 34-35 -0.38674636850362276 2313 36-37 -0.6869467733597148 2313 38-39 -0.18377336374464903 2313 40-41 0.1836828193395732 2313 42-43 0.2577581673794498 2313 44-45 0.16616852350832545 2313 46-47 0.5616599916088632 2313 48-49 0.763784326449322 2313 50-51 0.9084999067291655 2313 52-53 0.47389496073058623 2313 54-55 -0.24269869872916772 2313 56-57 0.14595333040640668 2313 58-59 -0.1448374769739118 2313 60-61 -0.4540565882212313 2313 62-63 -0.3008385250893575 2313 64-65 -0.6322060901065214 2313 66-67 0.07806635423942154 2313 68-69 0.013886362447383505 2313 70-71 0.01845837514061799 2313 72-73 0.170333808374302 2313 74-75 -0.030611505568113984 2313 76-77 -0.48884028147569936 2313 78-79 -0.22286259077057125 2313 80-81 -0.7401861549615099 2313 82-83 -0.5240840440187604 2313 84-85 0.0013547063899501666 2313 86-87 0.35226256631920094 2313 88-89 0.43281745418939366 2313 90-91 0.02979054012300253 2313 92-93 0.04077047213382201 2313 94-95 -0.21684403203346037 2313 96-97 0.2593264653758247 2313 98-99 0.4129132419813395 2313 100-101 0.9233293634417521 2314 1 -0.614502846278107 2314 2 -0.5955732430593486 2314 3 -0.4776056002087046 2314 4 -0.8044989478600115 2314 5 -0.7628007265111876 2314 6 -0.7828984281294211 2314 7 -0.7603221378798253 2314 8 -0.7863358240615668 2314 9 -1.6124885978829795 2314 10-11 -0.6016414208084271 2314 12-13 -0.7634222227634382 2314 14-15 -1.3080782768773318 2314 16-17 -0.8317126282153353 2314 18-19 -0.4808802886450749 2314 20-21 -1.0889200585626924 2314 22-23 -0.479237391726997 2314 24-25 -0.531344136098646 2314 26-27 -1.3249188421532025 2314 28-29 -1.8596712008961873 2314 30-31 -0.8964401640537787 2314 32-33 -0.3669870550644916 2314 34-35 -0.666017981846025 2314 36-37 -0.48493860453739046 2314 38-39 -0.8739537585710622 2314 40-41 -0.5555530554051558 2314 42-43 -0.1955689258812754 2314 44-45 0.01721515386911676 2314 46-47 -0.4145568225504306 2314 48-49 -0.0341649587787245 2314 50-51 0.044944767178453304 2314 52-53 -0.14531879012033144 2314 54-55 -0.4645758260266497 2314 56-57 -0.8476647771497525 2314 58-59 -1.3208500705750055 2314 60-61 -1.0356597196031245 2314 62-63 -0.9152445836325853 2314 64-65 -0.8348099022236042 2314 66-67 -0.6198148574692226 2314 68-69 -0.6293658499419941 2314 70-71 -0.7619194328920607 2314 72-73 -0.45662330530847584 2314 74-75 -0.5708599739139224 2314 76-77 0.07289389143103975 2314 78-79 -0.356669602343068 2314 80-81 0.13949389949730318 2314 82-83 -0.013660286360487106 2314 84-85 -0.05301901723155922 2314 86-87 0.1853122599883612 2314 88-89 -0.409890170044779 2314 90-91 -0.22629353067345903 2314 92-93 -0.2458615904938135 2314 94-95 2.679489059538298E-4 2314 96-97 -0.11133384776236355 2314 98-99 0.0732263801705848 2314 100-101 -0.6259388462246882 2315 1 -0.07467983742855111 2315 2 -0.14424580943103038 2315 3 0.0710669661629737 2315 4 0.09815591939662482 2315 5 0.13985414074544877 2315 6 0.09320776656084462 2315 7 0.09808494176618865 2315 8 -0.8748313992828045 2315 9 -0.09036470407766473 2315 10-11 -0.36270336771108447 2315 12-13 -0.22359921391388582 2315 14-15 -0.224007480417157 2315 16-17 -0.06180112379055913 2315 18-19 0.41734979985049847 2315 20-21 0.7562126848001469 2315 22-23 0.21102809499866737 2315 24-25 0.45095674885710224 2315 26-27 0.42729354722732893 2315 28-29 -0.08091013894928523 2315 30-31 0.15665718107896254 2315 32-33 0.39849967059922164 2315 34-35 0.3693802482424644 2315 36-37 1.0681587405953579 2315 38-39 0.7189665954112385 2315 40-41 0.9267478295505995 2315 42-43 1.2336346139417387 2315 44-45 0.9375691361700049 2315 46-47 0.48809804470620577 2315 48-49 1.0941536252920692 2315 50-51 1.239635032665177 2315 52-53 0.9033537762513468 2315 54-55 1.2478135545043223 2315 56-57 0.9310962847971496 2315 58-59 1.329592407301103 2315 60-61 1.0749597494234244 2315 62-63 1.3989147084028133 2315 64-65 1.1828892128206405 2315 66-67 1.596999301822816 2315 68-69 1.5387757429783555 2315 70-71 1.6849832219751981 2315 72-73 1.7203678451339997 2315 74-75 1.3760426809533293 2315 76-77 1.6481151303690922 2315 78-79 1.422091459603834 2315 80-81 1.2810868198512857 2315 82-83 1.911118474701457 2315 84-85 2.1195473544498533 2315 86-87 2.0127458883069487 2315 88-89 1.8644461131410637 2315 90-91 1.915299389680527 2315 92-93 0.9134304449044173 2315 94-95 1.5002679489059538 2315 96-97 2.4904360637420595 2315 98-99 2.759067088135186 2315 100-101 1.8829107112974377 2316 1 -0.43751169583563154 2316 2 -0.852210411200943 2316 3 -0.2563666621556102 2316 4 -0.19387947883345902 2316 5 0.32569484871005017 2316 6 0.5445352001891592 2316 7 0.47861591521751734 2316 8 0.656142052044629 2316 9 0.3255644994621534 2316 10-11 0.44260636680218823 2316 12-13 0.06401140555514928 2316 14-15 -0.09126411758530395 2316 16-17 -0.3494117432595871 2316 18-19 0.023544490115988026 2316 20-21 0.07479675559660848 2316 22-23 0.15793074986591904 2316 24-25 0.21201869575975962 2316 26-27 0.1529572640414898 2316 28-29 0.04740844512150488 2316 30-31 -0.042457863168827714 2316 32-33 0.12858816617444546 2316 34-35 0.050796177446002844 2316 36-37 -0.11325718860818768 2316 38-39 -0.4712988913144258 2316 40-41 -0.48033181646709977 2316 42-43 -0.5008786603945481 2316 44-45 -1.0226078549804427 2316 46-47 -1.5738488579486578 2316 48-49 -1.5208906224955392 2316 50-51 -1.7913384186622565 2316 52-53 -1.640894011359272 2316 54-55 -1.0574961800089504 2316 56-57 -0.7326205293621406 2316 58-59 -1.0066907785396069 2316 60-61 -0.20380131252347766 2316 62-63 -0.5391383889423196 2316 64-65 -0.37463291107316365 2316 66-67 -0.49149627339842183 2316 68-69 -1.0408702747207563 2316 70-71 -0.9256362470513473 2316 72-73 -0.4787471991137835 2316 74-75 -0.25670068187852735 2316 76-77 -1.024451241312324 2316 78-79 -0.6885280094227184 2316 80-81 -0.5905945960779171 2316 82-83 -0.1065806403427878 2316 84-85 -0.3229305216563354 2316 86-87 -0.7040682709850863 2316 88-89 -0.5205096390713244 2316 90-91 -0.7351430881955814 2316 92-93 -0.9095784046531072 2316 94-95 -0.42893559091705313 2316 96-97 -0.18655508670041954 2316 98-99 -0.14358777912145015 2316 100-101 -0.7631069878176078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3537.0 3 598.0 4 491.0 5 393.0 6 364.0 7 391.0 8 416.0 9 447.0 10 518.0 11 720.0 12 991.0 13 1210.0 14 1264.0 15 1336.0 16 1643.0 17 1960.0 18 1463.0 19 949.0 20 845.0 21 907.0 22 948.0 23 977.0 24 1039.0 25 1159.0 26 1314.0 27 1448.0 28 1699.0 29 1904.0 30 2271.0 31 2665.0 32 3128.0 33 3868.0 34 5073.0 35 6973.0 36 9850.0 37 14707.0 38 13901.0 39 2422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.221264848586245 19.319892922871006 14.538020746193741 20.920821482349005 2 16.531352204215125 22.11842888937651 38.47214820852011 22.87807069788826 3 20.140840655724002 26.301052125669987 31.00793012297437 22.55017709563164 4 13.120578492533728 17.73409824759397 37.14014023428112 32.00518302559119 5 12.787015457291265 37.504049831213486 33.6966859316702 16.012248779825047 6 28.95022662238653 36.18752219228439 17.966873446540095 16.89537773878898 7 24.75743871998663 35.335094883497476 22.145400996355054 17.762065400160836 8 25.177301260692914 33.07673828349401 20.92311548866212 20.822844967150957 9 26.094585561497325 16.860586564171122 22.27502506684492 34.769802807486634 10-11 23.960123035787955 27.15351795664503 27.383296168448318 21.503062839118698 12-13 26.123378386847442 25.70296016210909 27.84265390962836 20.33100754141511 14-15 23.041450587786777 26.567126764535388 28.120805719627533 22.270616928050302 16-17 20.1406876671123 29.83737884358289 28.67438168449198 21.347551804812834 18-19 19.960413413481373 27.992103572715543 32.175852182223636 19.87163083157944 20-21 21.029596862220785 27.160487379421642 31.772625905480144 20.03728985287743 22-23 20.740466029762807 27.056574404404355 31.685009428196835 20.517950137636003 24-25 20.526189306947245 27.39820493798782 32.03945374946451 20.036152005600428 26-27 20.53935730593011 27.328342380089126 32.1237324528637 20.00856786111706 28-29 20.166227850217318 27.627674690738885 32.315174690738885 19.890922768304915 30-31 20.289908065190136 27.730881738403678 31.785415796071874 20.19379440033431 32-33 19.930016973495235 27.933672803238018 31.591591591591587 20.544718631675156 34-35 20.63378217075367 27.523925198416855 31.395409712478795 20.446882918350678 36-37 20.585551570071107 27.1899188219747 31.570805277620455 20.65372433033373 38-39 20.36153547724151 27.288693628042065 31.741305008860138 20.608465885856287 40-41 20.417138424120434 26.93630226024238 31.803790833228497 20.842768482408687 42-43 20.237620855267295 27.39665694930895 31.177513055514776 21.188209139908977 44-45 20.283365144618998 27.14044507833966 31.15286196698618 21.423327810055163 46-47 20.53946913049406 27.127096158484786 30.477803530041005 21.855631180980147 48-49 20.538814126433046 27.13160406549261 30.457630140865753 21.87195166720859 50-51 20.31442550223653 27.27730378558656 30.456693078548692 21.951577633628215 52-53 20.479014909561943 27.372655139682088 30.10388970175632 22.04444024899965 54-55 20.200944944650995 26.99227578540003 30.374044960906982 22.432734309041997 56-57 20.159590642661662 27.232643731191473 30.012372993320678 22.59539263282618 58-59 20.304964985113433 26.849813393718286 29.976516962301336 22.868704658866942 60-61 20.453056797161974 26.413640960610362 30.551727390390752 22.58157485183691 62-63 20.246740104966783 26.428350469519263 30.338651580531334 22.986257844982617 64-65 19.91146264961658 26.742161192526776 30.25008654421098 23.096289613645663 66-67 19.880200574869395 26.77181461511025 29.732182195833246 23.615802614187107 68-69 20.020772462848242 26.42299238867586 29.754979358676437 23.801255789799463 70-71 20.015841459512483 26.493529655526938 29.433117043207318 24.05751184175326 72-73 20.002307797207568 26.178287824271735 29.31164703290709 24.507757345613612 74-75 20.10877546310865 26.12236977363795 29.0247970293914 24.744057733862 76-77 20.128579594019897 25.69389456683045 29.254479572520044 24.92304626662961 78-79 19.888231254357773 25.917033200350193 29.327762370839473 24.866973174452557 80-81 20.091958602900313 25.91932388931414 29.057660245991883 24.931057261793665 82-83 20.03922062123786 25.662241238281002 29.07516936177353 25.223368778707606 84-85 20.107909142389733 25.034082090647875 29.225654900480297 25.6323538664821 86-87 20.429876354178685 24.835610835526936 29.341499480876323 25.393013329418057 88-89 20.4792297082562 24.72611324673145 29.302867092159158 25.491789952853196 90-91 20.51794395758272 24.433466018450048 29.617625618192022 25.430964405775214 92-93 20.605326368566242 24.275989385023653 29.562499344430808 25.556184901979297 94-95 20.644494679037663 23.998615343463005 29.545633349242895 25.811256628256434 96-97 20.730619951746565 23.513584391062626 29.66747089059058 26.08832476660023 98-99 20.801690861794075 23.587626919526727 29.57854325753126 26.03213896114794 100-101 21.425350044574966 22.929361791389166 30.127956368975823 25.517331795060045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 150.0 1 102.0 2 63.5 3 75.0 4 66.5 5 64.5 6 104.0 7 172.5 8 226.0 9 248.5 10 239.0 11 209.5 12 211.5 13 235.5 14 249.5 15 258.0 16 271.0 17 308.0 18 330.5 19 339.5 20 383.0 21 449.5 22 516.5 23 564.5 24 614.0 25 736.5 26 911.5 27 1038.0 28 1198.0 29 1300.5 30 1353.0 31 1538.0 32 1728.0 33 1824.0 34 1945.0 35 2034.0 36 2190.5 37 2325.0 38 2309.0 39 2364.0 40 2467.0 41 2530.5 42 2595.0 43 2778.5 44 2948.0 45 3102.0 46 3301.0 47 3476.0 48 3671.5 49 3761.0 50 3645.5 51 3434.5 52 3189.0 53 3031.0 54 2823.0 55 2573.0 56 2306.5 57 2030.0 58 1845.0 59 1605.0 60 1389.0 61 1182.0 62 990.0 63 787.5 64 595.0 65 486.0 66 383.0 67 323.5 68 259.5 69 192.5 70 169.5 71 148.5 72 115.0 73 93.5 74 87.0 75 72.5 76 53.0 77 42.5 78 30.5 79 22.0 80 20.5 81 15.5 82 14.5 83 12.5 84 5.5 85 4.0 86 4.5 87 3.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1639019094050465 2 0.08978066375053503 3 0.08142897409932247 4 0.09604443098894445 5 0.11065988787856643 6 0.03653864222405495 7 0.041758448256062806 8 0.050110137907275365 9 0.046978254288070655 10-11 0.04645627368486987 12-13 0.05324202152648008 14-15 0.05063211851047615 16-17 0.046978254288070655 18-19 0.05167607971687772 20-21 0.054807963336082434 22-23 0.06837945901930284 24-25 0.08560481892492874 26-27 0.08612679952812954 28-29 0.0803850128929209 30-31 0.07203332324170834 32-33 0.05376400212968086 34-35 0.29544102141164436 36-37 0.4624748144358956 38-39 0.43637578427585627 40-41 0.4186284437670296 42-43 0.4457714351334704 44-45 0.454123124784683 46-47 0.4551670859910846 48-49 0.46978254288070653 50-51 0.45986491141989166 52-53 0.4671726398647026 54-55 0.45882095021349006 56-57 0.43846370668865947 58-59 0.4186284437670296 60-61 0.3867876269717817 62-63 0.44420549332386805 64-65 0.48231007735752535 66-67 0.48335403856392695 68-69 0.4911837476119387 70-71 0.4901397864055372 72-73 0.48022215494472226 74-75 0.4750023489127144 76-77 0.45882095021349006 78-79 0.43167795884704924 80-81 0.43846370668865947 82-83 0.4499472799590767 84-85 0.4499472799590767 86-87 0.4551670859910846 88-89 0.4682166010711042 90-91 0.4703045234839073 92-93 0.47187046529350973 94-95 0.4786562131351199 96-97 0.47917819373832066 98-99 0.4729144264999112 100-101 0.4635187756422971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 95789.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.6420570211611 #Duplication Level Percentage of deduplicated Percentage of total 1 91.24327328768854 75.40531793838541 2 6.038250675829312 9.98026913319901 3 1.284707309062429 3.18512564073119 4 0.517925267173644 1.712096378498575 5 0.29054344256082465 1.2005553873618058 6 0.1440084889214522 0.714069465178674 7 0.11369091230640964 0.6576955600329891 8 0.0884262651272075 0.5846182755848792 9 0.07326747681968622 0.5449477497416196 >10 0.18695838912609586 2.8061677228074204 >50 0.007579394153760642 0.4040129868774076 >100 0.00884262651272075 1.3101713140339704 >500 0.002526464717920214 1.4949524475670484 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 768 0.8017622065164058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 664 0.6931902410506426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 430 0.4489033187526752 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160 0.16703379302425123 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 147 0.1534622973410308 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 139 0.14511060768981823 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.14302268527701512 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.14093476286421197 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.111703849084968 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.21980603200785E-4 0.0 18-19 0.0 0.0 0.0 0.00104396120640157 0.0 20-21 0.0 0.0 0.0 0.00208792241280314 0.0 22-23 0.0 0.0 0.0 0.007829709048011776 0.0 24-25 0.0 0.0 0.0 0.015137437492822767 0.0 26-27 0.0 0.0 0.0 0.03392873920805103 0.0 28-29 0.0 0.0 0.0 0.08821472194093267 0.0 30-31 0.0 0.0 0.0 0.19261084258108968 0.0 32-33 0.0 0.0 0.0 0.35912265500214013 0.0 34-35 0.0 0.0 0.0 0.6347284134921547 0.0 36-37 0.0 0.0 0.0 0.9886312624622869 0.0 38-39 0.0 0.0 0.0 1.4636336113750013 0.0 40-41 0.0 0.0 0.0 1.9610811262253496 0.0 42-43 0.0 0.0 0.0 2.4047646389460167 0.0 44-45 0.0 0.0 0.0 2.952844272306841 0.0 46-47 0.0 0.0 0.0 3.5484241405589367 0.0 48-49 0.0 0.0 0.0 4.074058607982128 0.0 50-51 0.0 0.0 0.0 4.624226163755755 0.0 52-53 0.0 0.0 0.0 5.162388165655765 0.0 54-55 0.0 0.0 0.0 5.754836150288655 0.0 56-57 0.0 0.0 0.0 6.424015283592062 0.0 58-59 0.0 0.0 0.0 7.109375815594692 0.0 60-61 0.0 0.0 0.0 7.850066291536606 0.0 62-63 0.0 0.0 0.0 8.575619329985699 0.0 64-65 0.0 0.0 0.0 9.305870193863596 0.0 66-67 0.0 0.0 0.0 10.152000751652068 0.0 68-69 0.0 0.0 0.0 11.097829604651892 0.0 70-71 0.0 0.0 0.0 12.010251699046863 0.0 72-73 0.0 0.0 0.0 12.8981407050914 0.0 74-75 0.0 0.0 0.0 13.78341980811993 0.0 76-77 0.0 0.0 0.0 14.750127885247785 0.0 78-79 0.0 0.0 0.0 15.797220975268559 0.0 80-81 0.0 0.0 0.0 16.92730898119826 0.0 82-83 0.0 0.0 0.0 17.99267139233106 0.0 84-85 0.0 0.0 0.0 19.167127749532828 0.0 86-87 0.0 5.21980603200785E-4 0.0 20.298781697272126 0.0 88-89 0.0 0.00104396120640157 0.0 21.45653467517147 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTACC 25 0.0050667957 56.830006 9 GGACACT 35 2.8162464E-4 54.123817 6 ATGGACA 40 5.428825E-4 47.383106 4 GTATCAA 1045 0.0 28.58084 1 GGTATCA 735 0.0 28.38024 1 TGATCCA 35 0.008325283 27.175552 58-59 TGCACAA 90 9.0162124E-4 26.323946 5 TATCAAC 1250 0.0 23.881084 2 CAACGCA 1285 0.0 23.59937 5 TCAACGC 1285 0.0 23.59937 4 ATCAACG 1270 0.0 23.505007 3 AACGCAG 1275 0.0 23.40059 6 TTAATCC 105 0.0022130953 22.563383 4 AGAGTAC 1015 0.0 21.462893 10-11 CGCAGAG 1415 0.0 20.75065 8 ACGCAGA 1430 0.0 20.201809 7 GCAGAGT 1445 0.0 19.992102 9 GAGTACA 510 0.0 19.5209 1 AGTACTT 690 0.0 19.217875 12-13 GTACATG 525 0.0 18.963161 1 >>END_MODULE