FastQCFastQC Report
Thu 26 May 2016
SRR938837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95789
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT27452.86567351157231No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT13481.4072597062293164RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.7589597970539415No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT7020.7328607668939022RNA PCR Primer, Index 31 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.6796187453674222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.5084091075175646No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT2720.2839574481412271RNA PCR Primer, Index 31 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC2180.2275835429955423No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG2110.22027581455073128RNA PCR Primer, Index 31 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG1920.20044055162910146RNA PCR Primer, Index 38 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.19104490077148734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.15868210337303865No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1470.1534622973410308RNA PCR Primer, Index 31 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.14928645251542452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1200.1252753447681884No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.12423138356178684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG250.004933190557.21481
TACGAGA250.005036568756.916032
GTTATAG459.404885E-442.3813361
GGCAGTA459.6506265E-442.1600237
AGGTAAA459.6506265E-442.1600239
CTTTACA600.00386118431.7860011
GTAGCCG300.00397136131.62001862-63
ATGAGTT350.00826427827.2165772-73
CTGAGAC700.00841416627.1028753
TAAACTG908.964365E-426.3500145
GGTATCA8200.023.83951
GTATCAA10500.022.25021
CAACGCA12100.018.8152185
AACGCAG12150.018.737796
TATCAAC12250.018.5848272
TCAACGC12250.018.5848274
TTAAACT1300.007578049318.242324
ATCAACG12550.018.1405683
AGTACTT6450.018.01605812-13
CTTGAAA7950.017.90747556-57
Produced by FastQC (version 0.11.5)