FastQCFastQC Report
Thu 26 May 2016
SRR938836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95135
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.853523939664687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.6453986440321648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.42571083197561366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.175540022073895No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.15136385136910707No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.14085247280180796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.13139223209123876No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.10406264781626111No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT980.10301150995953119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATATG255.2757554E-575.926243
TCGCGCT300.00393093131.68601460-61
CTTTAAG650.005845117429.2023982
CATGGGC1004.9386137E-528.472344
GGTATCA7600.027.4877911
GTATCAA10400.026.4786241
GGTCGCC659.957186E-625.57905294-95
TATCAAC11950.023.0320192
AACGCAG12350.022.2860436
ATCAACG12200.022.1710853
TCAACGC12450.021.7258834
CAACGCA12700.021.2982065
GTCGCCG905.127591E-621.1128794-95
AGTACTT7700.020.95366912-13
GAGTACT7450.020.70136612-13
ACATGGG3700.020.5206053
ACGCAGA13650.020.163567
GTACATG4050.019.9294191
CTCCTGA1200.004744105519.782881
TACATGG3950.019.2218322