FastQCFastQC Report
Thu 26 May 2016
SRR938836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95135
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT24252.549009302570032No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT13161.3832974194565617RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.7620749461291848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.6863930204446314No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT5900.6201713354706471RNA PCR Primer, Index 31 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.46880748410153994No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG2950.31008566773532353RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT2590.2722447048930467RNA PCR Primer, Index 31 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC2520.26488673989593736No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG1960.20602301991906238RNA PCR Primer, Index 38 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.175540022073895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.15977295422294632No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1470.1545172649392968RNA PCR Primer, Index 31 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1471592999421874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.13244336994796868No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1070.11247175067010037No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.11036947495664055No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC960.10090923424607137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACA250.005020565456.9615144
GAGTAGA700.008381166527.1245291
GGTATCA8200.026.628351
GTATCAA11500.026.4169331
ACAGTCT908.922207E-426.371078
TATCAAC13500.022.15172
AGTACTT6550.022.10338612-13
ATCAACG13750.021.7489433
TCAACGC13800.021.6701414
CAACGCA13950.021.437135
AACGCAG14050.021.2845526
GAGTACT6500.020.81286212-13
AGAGTAC10700.019.51952210-11
ACGCAGA15350.019.481957
CGCAGAG15050.019.2394928
TACTTTT7950.018.50950814-15
ACAAGCA1559.579739E-418.3746833
GTACATG4400.018.339881
TTCTATT650.007569966418.25689552-53
ATACTCT1300.00754285518.2568955
Produced by FastQC (version 0.11.5)