##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938836_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95135 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92053397803122 31.0 31.0 34.0 30.0 34.0 2 32.123456141272925 33.0 31.0 34.0 30.0 34.0 3 32.18469543280601 34.0 31.0 34.0 30.0 34.0 4 35.836873916014085 37.0 35.0 37.0 35.0 37.0 5 35.740905029694645 37.0 35.0 37.0 33.0 37.0 6 35.78038576759342 37.0 35.0 37.0 33.0 37.0 7 35.685121143637986 37.0 35.0 37.0 33.0 37.0 8 35.703894465759184 37.0 35.0 37.0 33.0 37.0 9 37.28645608871604 39.0 37.0 39.0 34.0 39.0 10-11 37.238256162295684 39.0 37.0 39.0 33.0 39.0 12-13 37.14580858779629 39.0 37.0 39.0 33.0 39.0 14-15 38.27548220949177 40.0 38.0 41.0 33.0 41.0 16-17 38.200746307878276 40.0 37.5 41.0 33.0 41.0 18-19 38.32658853208598 40.0 38.0 41.0 33.0 41.0 20-21 38.36623745204183 40.0 38.0 41.0 33.5 41.0 22-23 38.25109581121564 40.0 37.5 41.0 33.0 41.0 24-25 38.09064487307511 40.0 37.0 41.0 33.0 41.0 26-27 37.847837283859775 40.0 37.0 41.0 33.0 41.0 28-29 37.5712303568613 40.0 36.0 41.0 32.0 41.0 30-31 37.19463919693068 39.0 36.0 41.0 31.5 41.0 32-33 36.842140116676305 39.0 35.0 41.0 31.0 41.0 34-35 36.554107321175174 39.0 35.0 41.0 30.5 41.0 36-37 36.2177116728859 39.0 35.0 41.0 30.0 41.0 38-39 35.86366741998213 39.0 35.0 41.0 28.5 41.0 40-41 35.59754033741525 39.0 35.0 41.0 27.5 41.0 42-43 35.209602144321224 39.0 35.0 41.0 25.5 41.0 44-45 34.75790192883797 39.0 35.0 41.0 23.5 41.0 46-47 34.12097020024176 38.0 34.0 40.5 17.5 41.0 48-49 33.61528354443685 38.0 33.5 40.0 9.5 41.0 50-51 33.115577863036734 38.0 33.0 40.0 2.0 41.0 52-53 32.828943080885054 38.0 33.0 40.0 2.0 41.0 54-55 32.449082882220004 37.0 32.5 40.0 2.0 41.0 56-57 31.93179692016608 37.0 31.5 40.0 2.0 41.0 58-59 31.37915068061176 36.5 31.0 40.0 2.0 41.0 60-61 30.72530088821149 36.0 30.0 40.0 2.0 41.0 62-63 30.17871445840122 35.5 29.0 39.0 2.0 41.0 64-65 29.561749093393598 35.0 28.0 39.0 2.0 40.5 66-67 28.875781783780944 35.0 26.5 38.5 2.0 40.0 68-69 28.38872129079729 35.0 26.0 38.0 2.0 40.0 70-71 27.96500236506018 35.0 25.5 37.0 2.0 40.0 72-73 27.51622956850791 34.0 24.5 37.0 2.0 39.0 74-75 27.09163294266043 34.0 23.0 36.0 2.0 39.0 76-77 25.359010879276816 31.5 20.0 34.5 2.0 36.5 78-79 26.13029379303096 33.5 20.0 35.0 2.0 37.0 80-81 26.156430335838547 34.0 20.0 35.0 2.0 37.0 82-83 25.863367845692963 33.5 18.5 35.0 2.0 36.5 84-85 25.51936721501025 33.0 16.5 35.0 2.0 36.0 86-87 25.03315288800126 33.0 7.5 35.0 2.0 36.0 88-89 24.5072318284543 32.5 2.0 35.0 2.0 35.0 90-91 24.03349976349398 32.0 2.0 35.0 2.0 35.0 92-93 23.774678089031376 32.0 2.0 35.0 2.0 35.0 94-95 23.5182897987071 32.0 2.0 34.5 2.0 35.0 96-97 22.996431386976404 31.5 2.0 34.0 2.0 35.0 98-99 22.341882587901402 31.0 2.0 34.0 2.0 35.0 100-101 19.627408419614234 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08914279305419015 1101 2 0.04520697249408556 1101 3 -0.15869167801884743 1101 4 0.0938396049174628 1101 5 0.0948977781175131 1101 6 0.06687373415498143 1101 7 0.08587847860747644 1101 8 0.055338046522052764 1101 9 0.012994731581216001 1101 10-11 0.4791877483998874 1101 12-13 0.05769741357065783 1101 14-15 -0.3164025967108728 1101 16-17 0.4683205006662945 1101 18-19 0.5570782848128601 1101 20-21 0.4518873736543867 1101 22-23 0.12202322523868503 1101 24-25 0.27024574450010874 1101 26-27 -0.022063928760495344 1101 28-29 -0.051246929267797725 1101 30-31 -0.5299620070053379 1101 32-33 -0.5420135020161538 1101 34-35 -0.7931422563163579 1101 36-37 -0.8640225909654831 1101 38-39 -1.21932900159117 1101 40-41 -0.7103036816764998 1101 42-43 -0.8832070500114071 1101 44-45 -1.2053473405078918 1101 46-47 -1.28321703287655 1101 48-49 -1.2573917837195694 1101 50-51 -2.627841992986241 1101 52-53 -2.388694693698433 1101 54-55 -2.379054781362715 1101 56-57 -1.7315052121652421 1101 58-59 -1.5689801790808602 1101 60-61 -1.605872889347058 1101 62-63 -1.614890077270939 1101 64-65 -0.955175086476526 1101 66-67 -1.5415422291578587 1101 68-69 -1.027813398392027 1101 70-71 -1.0610558416202416 1101 72-73 -0.9791709332296783 1101 74-75 -1.3036346459858734 1101 76-77 -1.085734249571182 1101 78-79 -1.842394015640064 1101 80-81 -2.0822663832633843 1101 82-83 -1.541841170061467 1101 84-85 -1.574421678884974 1101 86-87 -1.1017707870593014 1101 88-89 -1.202940204868657 1101 90-91 -2.2411157883714097 1101 92-93 -2.8815838882232256 1101 94-95 -2.711807049495171 1101 96-97 -2.9870826528967527 1101 98-99 -3.2713769754673727 1101 100-101 -2.2741463307102556 1102 1 -0.1614494685333696 1102 2 0.21546473852184533 1102 3 -0.14904197544119313 1102 4 0.006595718598894962 1102 5 -0.4166686462050251 1102 6 -0.16485131541541165 1102 7 -0.07578708649496946 1102 8 -0.5301213057581791 1102 9 -0.47530665638970504 1102 10-11 -0.21889552985523153 1102 12-13 -0.7434261819349572 1102 14-15 -0.006885081839755003 1102 16-17 -0.6484673380646981 1102 18-19 -0.5176077693840995 1102 20-21 0.032323593085976654 1102 22-23 -0.4868994449529893 1102 24-25 -0.40582828061555887 1102 26-27 -0.6531941336514464 1102 28-29 -0.20954170785338988 1102 30-31 0.109297741838013 1102 32-33 -0.8143862713486101 1102 34-35 -1.170141595377828 1102 36-37 -1.3931699802582784 1102 38-39 -1.9478815462045134 1102 40-41 -1.2672765831966544 1102 42-43 -1.155051068517679 1102 44-45 -1.3836685566347953 1102 46-47 -1.063851533867954 1102 48-49 -1.4098703032172537 1102 50-51 -2.2257269896815473 1102 52-53 -1.9058130016957904 1102 54-55 -2.2074751382695226 1102 56-57 -1.1526552452121699 1102 58-59 -1.0860984870782175 1102 60-61 -1.1642998556391895 1102 62-63 -0.5716646972312809 1102 64-65 -0.5317778624183624 1102 66-67 -0.7103459304136415 1102 68-69 -1.2594723541091462 1102 70-71 -1.025695630119909 1102 72-73 -1.561193405139793 1102 74-75 -0.8684066221920865 1102 76-77 -0.7631301517522786 1102 78-79 -0.8774237578740376 1102 80-81 -0.3584725960855941 1102 82-83 -0.8532093128241911 1102 84-85 -0.7570389954745309 1102 86-87 -0.8183603442304843 1102 88-89 -0.28853174485543676 1102 90-91 -1.3497079892967214 1102 92-93 -1.4205792616534936 1102 94-95 -1.6531818016432211 1102 96-97 -1.7520529106627833 1102 98-99 -1.0186340805565983 1102 100-101 -1.41241467175454 1103 1 0.19810109326438052 1103 2 0.09186923290386773 1103 3 0.07567712568240381 1103 4 -0.09452787690672437 1103 5 0.06647742121070621 1103 6 -0.1536153603592325 1103 7 -0.16567472694440255 1103 8 0.09909217738788811 1103 9 0.2325585121496232 1103 10-11 0.28672244767285093 1103 12-13 0.2678097731212219 1103 14-15 0.4088452552389015 1103 16-17 0.4301843473285629 1103 18-19 -0.12996731994589794 1103 20-21 -0.2654292159027847 1103 22-23 -0.3801578719192804 1103 24-25 0.18405935983387423 1103 26-27 0.037817102303613126 1103 28-29 -0.7432495730219273 1103 30-31 -0.5985674267013081 1103 32-33 -0.20764469831490118 1103 34-35 -0.5016022695351268 1103 36-37 -0.6403609914942336 1103 38-39 -0.2624882877775505 1103 40-41 -0.44705186409553477 1103 42-43 0.20449949328006767 1103 44-45 0.27363481415172686 1103 46-47 0.4473844211882252 1103 48-49 0.8878825057715076 1103 50-51 0.7293291900937362 1103 52-53 0.9481195825738666 1103 54-55 0.33746868195519397 1103 56-57 0.7743110469226622 1103 58-59 1.0487329735959392 1103 60-61 1.1783967735742955 1103 62-63 0.5294588982743349 1103 64-65 0.6142895533119734 1103 66-67 1.0312271032942206 1103 68-69 1.560752364991977 1103 70-71 1.8507088642621135 1103 72-73 0.9781324375568339 1103 74-75 1.4630540519652158 1103 76-77 1.5627125448769377 1103 78-79 1.7798796129124774 1103 80-81 2.1190554938020476 1103 82-83 1.804094057962324 1103 84-85 2.602511566323223 1103 86-87 2.243437408578501 1103 88-89 2.5934907270546717 1103 90-91 2.2401796511527152 1103 92-93 1.899645457447626 1103 94-95 1.8468181983567789 1103 96-97 2.096261696078791 1103 98-99 2.0656355823647523 1103 100-101 2.7055628563353444 1104 1 -0.06032587302775383 1104 2 -0.08790604799500557 1104 3 -0.03668242487939466 1104 4 0.08524740399214892 1104 5 0.1563650616601464 1104 6 0.08233969582054357 1104 7 -0.01960731121406667 1104 8 -0.1480988338480671 1104 9 -0.09328418447959308 1104 10-11 0.11818312183016388 1104 12-13 -0.20410033923833026 1104 14-15 -0.0012671043116654346 1104 16-17 0.13804951586788405 1104 18-19 0.39250459016646033 1104 20-21 -0.007002249610650324 1104 22-23 -0.24532641124512367 1104 24-25 -0.17549120196387236 1104 26-27 0.17264856297776987 1104 28-29 -0.02976642695451659 1104 30-31 -0.6435112469260247 1104 32-33 -1.1627008780901846 1104 34-35 -0.7768831684115298 1104 36-37 -0.08418121621333796 1104 38-39 -0.10518491699102839 1104 40-41 -0.7279507405000274 1104 42-43 -0.053927473012066685 1104 44-45 -0.361196646522437 1104 46-47 -1.3335144552162674 1104 48-49 -0.6233534492846715 1104 50-51 -0.3774123829399798 1104 52-53 -0.8383972713587156 1104 54-55 -0.9378122169212091 1104 56-57 -0.7593968182458752 1104 58-59 -0.4737389365164191 1104 60-61 0.10536306570912402 1104 62-63 -0.021102899478474768 1104 64-65 0.8839524746602869 1104 66-67 0.41324957520433614 1104 68-69 0.4821006795987195 1104 70-71 0.41250661707110226 1104 72-73 1.3994807521635764 1104 74-75 1.3226046137629694 1104 76-77 0.7818136684724415 1104 78-79 0.7798796129124774 1104 80-81 1.0965835836896858 1104 82-83 0.6243187770634506 1104 84-85 0.6081295438513123 1104 86-87 0.6591677456571539 1104 88-89 0.2676480304254589 1104 90-91 -0.5351012477236878 1104 92-93 -0.05541072232765387 1104 94-95 0.3749080859972267 1104 96-97 0.22547517922485838 1104 98-99 0.16114120034227852 1104 100-101 0.6100572383578218 1105 1 0.35972598522635124 1105 2 0.3499257830952516 1105 3 0.4213988197187035 1105 4 0.21859720026329654 1105 5 0.1905668149630202 1105 6 0.13901335923578273 1105 7 0.23881965507806768 1105 8 0.2239224033417031 1105 9 0.36257456351398787 1105 10-11 0.11991173030033764 1105 12-13 0.3467084025816476 1105 14-15 -0.20024228643291053 1105 16-17 0.20632955044005996 1105 18-19 0.3760828096997315 1105 20-21 0.6220877615018381 1105 22-23 0.32486743984760125 1105 24-25 0.4075437406216267 1105 26-27 0.7635857157126722 1105 28-29 0.9005953461038843 1105 30-31 0.18807289926485282 1105 32-33 0.47324183088623073 1105 34-35 0.5823587133022556 1105 36-37 0.20585459067640954 1105 38-39 -0.2908868555019595 1105 40-41 -0.052126564675852194 1105 42-43 -0.6748683299079801 1105 44-45 -0.43398341032042964 1105 46-47 -0.29381819641888995 1105 48-49 -0.32998389748814816 1105 50-51 -0.2577062463799109 1105 52-53 0.41311526105269536 1105 54-55 0.4166142554827914 1105 56-57 0.6286757833098662 1105 58-59 0.3317308745713703 1105 60-61 -0.3344443179184857 1105 62-63 -0.16136712963034583 1105 64-65 -0.14172970832526488 1105 66-67 -0.525446560121015 1105 68-69 -0.002280108152856286 1105 70-71 0.03202754558079235 1105 72-73 -0.42642368048242574 1105 74-75 0.10134272957973778 1105 76-77 0.2034706631631451 1105 78-79 0.10072169218152638 1105 80-81 0.3504420847391998 1105 82-83 0.1304923787426695 1105 84-85 -0.15276686311251098 1105 86-87 0.4513643131346221 1105 88-89 0.4489667117441378 1105 90-91 0.31111433444694825 1105 92-93 0.9513185035026979 1105 94-95 0.8604618580678576 1105 96-97 0.29177966125967814 1105 98-99 0.8246799088248089 1105 100-101 1.7977347294060984 1106 1 0.09598972149008844 1106 2 -0.05666762349815713 1106 3 -0.24893085061096443 1106 4 -0.24294126127516336 1106 5 -0.1500925256963228 1106 6 0.018134238356658727 1106 7 0.22783064408905318 1106 8 0.1579883374076374 1106 9 0.16477236571179077 1106 10-11 0.08694469733330834 1106 12-13 0.011543567416808287 1106 14-15 0.11294452675390687 1106 16-17 0.11841746252797236 1106 18-19 -0.16237872876180148 1106 20-21 -0.026263886849811513 1106 22-23 0.40728502226519225 1106 24-25 0.5613898944677871 1106 26-27 0.4394098915368474 1106 28-29 0.5654305109390521 1106 30-31 0.9902707014626628 1106 32-33 0.7974176550620555 1106 34-35 0.32411695506050364 1106 36-37 0.24981063463244624 1106 38-39 -0.07110663572174047 1106 40-41 0.2665547540054618 1106 42-43 -0.25728591232556397 1106 44-45 0.6429396666026435 1106 46-47 0.49739059478989844 1106 48-49 0.8348512673470125 1106 50-51 1.165370676697016 1106 52-53 1.319708667646104 1106 54-55 1.141888980757514 1106 56-57 0.9583461129802018 1106 58-59 1.1449176877581806 1106 60-61 1.7864348029606383 1106 62-63 1.2562152879520738 1106 64-65 1.2648636982681403 1106 66-67 1.3426853080108536 1106 68-69 1.1076100017372532 1106 70-71 1.0375220510752996 1106 72-73 1.765884011825264 1106 74-75 1.5518921801291903 1106 76-77 1.3518223115147947 1106 78-79 1.166655758115592 1106 80-81 1.257035491332605 1106 82-83 1.6964264446767352 1106 84-85 1.990090279744635 1106 86-87 2.3140016757719835 1106 88-89 2.443472206249634 1106 90-91 2.3440813674139847 1106 92-93 1.7150547672389607 1106 94-95 1.3769453745513687 1106 96-97 1.7862851557651709 1106 98-99 1.8356689198138163 1106 100-101 2.418613850285219 1107 1 0.20420759057752136 1107 2 0.050833570959561314 1107 3 0.2516663782471369 1107 4 0.17440226476401222 1107 5 0.2016752717236514 1107 6 -0.18683470574271865 1107 7 0.11925443768676303 1107 8 -0.3950455851914967 1107 9 -0.24301549859781346 1107 10-11 -0.12925214929832407 1107 12-13 -0.09338954295107982 1107 14-15 0.1627534135193116 1107 16-17 -0.14107847631579773 1107 18-19 0.07466140501745855 1107 20-21 0.07854977774645988 1107 22-23 -0.002593143047754154 1107 24-25 -0.1202274012799478 1107 26-27 -0.24405881367098203 1107 28-29 -0.17113183975373403 1107 30-31 -0.7086065082841131 1107 32-33 -0.7381771848806054 1107 34-35 -0.741160159129663 1107 36-37 -0.2408128722954075 1107 38-39 0.04320296771830101 1107 40-41 0.23944056384780055 1107 42-43 0.5628287556151861 1107 44-45 0.9791795137120616 1107 46-47 1.1194044504994096 1107 48-49 0.06191767919602853 1107 50-51 0.6724777001083879 1107 52-53 0.08368382292560739 1107 54-55 -0.05053815732179601 1107 56-57 -0.04231083876370434 1107 58-59 0.10448529406491858 1107 60-61 0.2897792845659879 1107 62-63 0.5425268025244563 1107 64-65 0.7534566270784602 1107 66-67 0.7677928092053108 1107 68-69 0.8232096195107843 1107 70-71 0.40505669034907044 1107 72-73 0.10343776242249092 1107 74-75 0.3801888834258911 1107 76-77 0.6900330138559312 1107 78-79 0.4162376979149194 1107 80-81 0.4895366857903234 1107 82-83 0.7693480882505526 1107 84-85 0.8188647661278168 1107 86-87 0.11715504414274136 1107 88-89 0.5122132478167636 1107 90-91 0.6579251323447046 1107 92-93 0.8648994877358547 1107 94-95 1.9947786282541884 1107 96-97 2.4651551987656504 1107 98-99 2.373891566732116 1107 100-101 2.2146004723587964 1108 1 -0.11638737509753483 1108 2 -0.31392615444089955 1108 3 -0.3413542167185426 1108 4 -0.15260002356550473 1108 5 -0.11377095253264002 1108 6 -0.03900861878619821 1108 7 -0.05591718702719817 1108 8 0.13149903723412848 1108 9 -0.4780269402454138 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0.3402055620560418 2316 54-55 0.4559835818086384 2316 56-57 0.8283413351493394 2316 58-59 0.0947026853692634 2316 60-61 -0.14065549804270816 2316 62-63 0.062092019915763075 2316 64-65 0.4501957575132458 2316 66-67 -0.23438110383816735 2316 68-69 -0.25287733701095405 2316 70-71 -0.18842157052049302 2316 72-73 -0.4400404984470718 2316 74-75 -0.5013328557045433 2316 76-77 -0.5414887252745046 2316 78-79 -0.36745795425899175 2316 80-81 0.04279755535553775 2316 82-83 -0.5643475639233628 2316 84-85 -0.8191787121330556 2316 86-87 -0.2524101732485633 2316 88-89 0.028517595642849614 2316 90-91 -0.8605531285248595 2316 92-93 -0.3992309470467532 2316 94-95 -0.06935180652842021 2316 96-97 -0.13158393166913385 2316 98-99 -0.45219538978961893 2316 100-101 -0.7636603972064187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 6.0 6 19.0 7 58.0 8 103.0 9 206.0 10 418.0 11 599.0 12 844.0 13 1072.0 14 1172.0 15 1450.0 16 1667.0 17 2124.0 18 1864.0 19 1359.0 20 1259.0 21 1437.0 22 1550.0 23 1497.0 24 1674.0 25 1644.0 26 1266.0 27 1327.0 28 1532.0 29 1884.0 30 2295.0 31 2697.0 32 3265.0 33 3971.0 34 5276.0 35 7245.0 36 10617.0 37 16554.0 38 13791.0 39 1391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.45200716468233 21.362343272574016 14.991044147086713 23.194605415656937 2 15.341357018973037 22.118042781310766 40.27434698060651 22.266253219109686 3 18.327639669942712 27.220265937877752 33.70999106532822 20.742103326851318 4 12.208966205917907 20.68429074473117 34.561412729279446 32.54533032007147 5 11.5061925689173 38.7923964926299 35.01377294614883 14.687637992303973 6 27.309996846420688 37.914432881320295 16.93051613581415 17.845054136444865 7 22.91900982813896 36.07925579439743 20.413097177694855 20.58863719976875 8 24.279182214747465 34.856782467020544 21.590371577232354 19.273663740999634 9 23.63693698428549 17.014768486887057 20.649603195459086 38.69869133336837 10-11 22.819677300677984 27.575550533452464 27.920849319388235 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19.440297722924242 27.911524147936333 32.447803872921085 20.200374256218332 38-39 19.871121015894374 26.318223866790007 32.016335884282235 21.794319233033388 40-41 19.54357675205769 26.450894030337114 32.845234466157194 21.160294751448003 42-43 20.94029223168296 26.301902659518557 32.395669084410805 20.36213602438768 44-45 19.260828204575674 27.337373270193993 31.81476556628317 21.587032958947162 46-47 21.20039943238556 25.90792032375046 30.264886739895935 22.626793503968045 48-49 20.59347555303281 25.675753801197253 30.329165549826826 23.40160509594311 50-51 19.305723445629894 25.947337993377833 31.687076260051505 23.059862300940768 52-53 19.825511115782835 26.34887265464866 32.69669417144058 21.128922058127923 54-55 19.127555578914173 26.611131549902773 30.97387922426026 23.287433646922796 56-57 20.685976170579547 27.468478837855876 29.18604712275778 22.659497868806795 58-59 18.675664454009848 28.778729062537778 30.972465299129126 21.573141184323248 60-61 20.19267660708373 30.36469802961102 28.070027908150124 21.372597455155123 62-63 18.529029952330646 31.707740094287562 27.919419348396218 21.843810604985574 64-65 18.516416231756093 31.311459039570295 27.857401888904654 22.314722839768958 66-67 18.52379400603391 30.793974497787215 27.709741508898446 22.97248998728043 68-69 18.647185578388605 29.82288327114101 27.783675829084984 23.7462553213854 70-71 19.147001629263677 28.87843590686919 27.442581594576126 24.531980869291008 72-73 19.242129605297734 28.415409680979657 27.47253902349293 24.869921690229674 74-75 18.984800386822798 28.24542224651544 27.28099312548616 25.488784241175605 76-77 19.276360767814882 27.448330265579806 27.722850381277937 25.552458585327376 78-79 19.25730615595939 27.467357153006933 27.605243906931705 25.67009278410197 80-81 19.079929362793532 27.585826308154815 27.428153972291714 25.906090356759936 82-83 19.34882009775582 27.00268040153466 27.217112524307563 26.431386976401956 84-85 19.20744205602565 26.571188311347033 27.267041572502233 26.95432806012509 86-87 19.228017005514165 26.32539882981858 27.24828471608368 27.198299448583573 88-89 19.25330548655391 25.9681766160889 27.318323497302345 27.46019440005485 90-91 19.058170576007935 25.89239551349013 27.454021715045425 27.595412195456515 92-93 19.095495874283912 25.738687128816945 27.59236873916014 27.573448257739003 94-95 19.06879207858348 24.954143869994585 27.6413919240654 28.33567212735653 96-97 18.897356388290323 24.834183003100858 27.8404372733484 28.42802333526042 98-99 18.958847952909025 24.71330214957692 27.709045041257163 28.618804856256897 100-101 18.973563882903242 24.28181005938929 28.46113417774741 28.283491879960053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107.0 1 72.5 2 56.0 3 68.0 4 54.0 5 58.0 6 88.5 7 138.5 8 175.5 9 192.0 10 192.0 11 167.0 12 172.5 13 187.5 14 201.0 15 205.0 16 217.0 17 238.0 18 247.0 19 281.0 20 314.0 21 354.5 22 389.5 23 410.5 24 466.5 25 568.5 26 690.5 27 872.5 28 1021.5 29 1148.0 30 1274.5 31 1417.5 32 1655.5 33 1786.5 34 1969.0 35 2173.0 36 2325.0 37 2469.5 38 2477.5 39 2472.5 40 2541.5 41 2705.5 42 2920.0 43 3128.0 44 3321.0 45 3528.5 46 3727.0 47 3926.0 48 4058.0 49 3969.0 50 3858.0 51 3765.5 52 3513.5 53 3141.5 54 2774.0 55 2471.5 56 2186.0 57 1901.0 58 1645.0 59 1409.0 60 1188.5 61 996.0 62 768.5 63 561.5 64 438.0 65 333.5 66 253.0 67 205.5 68 152.5 69 103.5 70 73.5 71 54.5 72 42.5 73 35.0 74 26.0 75 21.5 76 15.5 77 10.5 78 9.5 79 6.5 80 5.5 81 7.5 82 7.0 83 4.0 84 1.5 85 0.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2365060177642298 2 0.0 3 0.0 4 0.0 5 0.022073894991328114 6 0.005255689283649551 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0015767067850948652 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007357964997109371 36-37 0.013664792137488833 38-39 0.007357964997109371 40-41 0.00420455142691964 42-43 0.005255689283649551 44-45 0.0015767067850948652 46-47 0.0 48-49 5.25568928364955E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.25568928364955E-4 58-59 5.25568928364955E-4 60-61 0.0015767067850948652 62-63 5.25568928364955E-4 64-65 5.25568928364955E-4 66-67 0.006306827140379461 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.00105113785672991 76-77 0.0630682714037946 78-79 0.13612235244652335 80-81 0.00105113785672991 82-83 0.0 84-85 0.0 86-87 0.11247175067010037 88-89 0.3474010616492353 90-91 0.3805119041362275 92-93 0.0 94-95 5.25568928364955E-4 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 95135.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.68613023598046 #Duplication Level Percentage of deduplicated Percentage of total 1 90.00333314815843 68.12003994323855 2 6.802399866674073 10.2969464445262 3 1.459641131048275 3.3142376622694063 4 0.62218765624132 1.8836390392599989 5 0.313871451586023 1.1877857781047985 6 0.21387700683295371 0.9712513796184369 7 0.11943780901061052 0.6327849897514058 8 0.10416087995111384 0.6306827140379461 9 0.06110771623798678 0.41625059126504443 >10 0.26804066440753294 3.533925474325958 >50 0.008332870396089105 0.49718820623324744 >100 0.01666574079217821 2.514321753297945 >500 0.0041664351980445526 2.0686393020444633 >1k 0.0027776234653630353 3.932306722026594 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2425 2.549009302570032 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1316 1.3832974194565617 RNA PCR Primer, Index 31 (95% over 22bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 0.7620749461291848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.6863930204446314 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 590 0.6201713354706471 RNA PCR Primer, Index 31 (95% over 24bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.46880748410153994 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 295 0.31008566773532353 RNA PCR Primer, Index 31 (96% over 29bp) CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 259 0.2722447048930467 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 252 0.26488673989593736 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 196 0.20602301991906238 RNA PCR Primer, Index 38 (95% over 24bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 167 0.175540022073895 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.15977295422294632 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 147 0.1545172649392968 RNA PCR Primer, Index 31 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.1471592999421874 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.13244336994796868 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 107 0.11247175067010037 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 0.11036947495664055 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 96 0.10090923424607137 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.25568928364955E-4 0.0 22-23 0.0 0.0 0.0 0.005255689283649551 0.0 24-25 0.0 0.0 0.0 0.01681820570767856 0.0 26-27 0.0 0.0 0.0 0.028380722131707573 0.0 28-29 0.0 0.0 0.0 0.06359384033215956 0.0 30-31 0.0 0.0 0.0 0.16660535029169077 0.0 32-33 0.0 0.0 0.0 0.3615914227150891 0.0 34-35 0.0 0.0 0.0 0.6401429547485153 0.0 36-37 0.0 0.0 0.0 1.0222315656698375 0.0 38-39 0.0 0.0 0.0 1.4957691701266622 0.0 40-41 0.0 0.0 0.0 1.964051085299837 0.0 42-43 0.0 0.0 0.0 2.4050034161980345 0.0 44-45 0.0 0.0 0.0 2.9284700688495295 0.0 46-47 0.0 0.0 0.0 3.4976612182687763 0.0 48-49 0.0 0.0 0.0 4.063173385189468 0.0 50-51 0.0 0.0 0.0 4.640773637462553 0.0 52-53 0.0 0.0 0.0 5.14006411940926 0.0 54-55 0.0 0.0 0.0 5.687181373837179 0.0 56-57 0.0 0.0 0.0 6.365690860356336 0.0 58-59 0.0 0.0 0.0 7.053135018657697 0.0 60-61 0.0 0.0 0.0 7.782624691228254 0.0 62-63 0.0 0.0 0.0 8.512114363798812 0.0 64-65 0.0 0.0 0.0 9.230041519945342 0.0 66-67 0.0 0.0 0.0 10.031534135701897 0.0 68-69 0.0 0.0 0.0 10.907131970357913 0.0 70-71 0.0 0.0 0.0 11.910968623534977 0.0 72-73 0.0 0.0 0.0 12.816523887107795 0.0 74-75 0.0 0.0 0.0 13.784096284227676 0.0 76-77 0.0 0.0 0.0 14.803174436327325 0.0 78-79 0.0 0.0 0.0 15.890576549114417 0.0 80-81 0.0 0.0 0.0 17.00268040153466 0.0 82-83 0.0 0.0 0.0 18.1373837178746 0.0 84-85 0.0 0.0 0.0 19.25106427707994 0.0 86-87 0.0 0.0 0.0 20.412046039838124 0.0 88-89 0.0 0.0 0.0 21.58143690545015 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTACA 25 0.0050205654 56.961514 4 GAGTAGA 70 0.0083811665 27.124529 1 GGTATCA 820 0.0 26.62835 1 GTATCAA 1150 0.0 26.416933 1 ACAGTCT 90 8.922207E-4 26.37107 8 TATCAAC 1350 0.0 22.1517 2 AGTACTT 655 0.0 22.103386 12-13 ATCAACG 1375 0.0 21.748943 3 TCAACGC 1380 0.0 21.670141 4 CAACGCA 1395 0.0 21.43713 5 AACGCAG 1405 0.0 21.284552 6 GAGTACT 650 0.0 20.812862 12-13 AGAGTAC 1070 0.0 19.519522 10-11 ACGCAGA 1535 0.0 19.48195 7 CGCAGAG 1505 0.0 19.239492 8 TACTTTT 795 0.0 18.509508 14-15 ACAAGCA 155 9.579739E-4 18.374683 3 GTACATG 440 0.0 18.33988 1 TTCTATT 65 0.0075699664 18.256895 52-53 ATACTCT 130 0.007542855 18.256895 5 >>END_MODULE