FastQCFastQC Report
Thu 26 May 2016
SRR938835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318484
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200550.464399080788536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140940.32636452977480057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99950.23144696148000085No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58330.13507054790523712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA270200.029.7219161
GGTATCA185500.028.8024041
GTACATG221900.028.5001581
TACATGG228900.027.261012
ACATGGG229500.025.3523163
ATCAACG327800.024.3697223
TCAACGC327350.024.3308534
TATCAAC330050.024.2323042
CAACGCA329650.024.1179755
AACGCAG334200.023.7164136
CATGGGA124550.022.786894
GAGTACT156150.022.18170712-13
AGTACTT162550.022.02203612-13
CATGGGG106550.021.5225584
ACGCAGA368000.021.2943947
CGCAGAG368500.021.1110658
AGAGTAC288450.019.83746510-11
GCAGAGT382950.019.7333539
GTACTTT175750.019.64117214-15
TACTTTT195850.018.22984514-15
Produced by FastQC (version 0.11.5)