##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4318484 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68893111564151 33.0 31.0 34.0 30.0 34.0 2 31.73830399742132 33.0 31.0 34.0 30.0 34.0 3 31.754569427604686 33.0 31.0 34.0 30.0 34.0 4 35.32142622272075 37.0 35.0 37.0 33.0 37.0 5 35.35371255283104 37.0 35.0 37.0 33.0 37.0 6 35.44668314158395 37.0 35.0 37.0 33.0 37.0 7 35.361003305789716 37.0 35.0 37.0 33.0 37.0 8 35.33208551889969 37.0 35.0 37.0 33.0 37.0 9 36.90637362555934 39.0 37.0 39.0 33.0 39.0 10-11 36.80458165411751 39.0 37.0 39.0 32.0 39.0 12-13 36.688486052049754 39.0 37.0 39.0 32.0 39.0 14-15 37.85226956959896 40.0 38.0 41.0 32.0 41.0 16-17 37.79253703382946 40.0 37.0 41.0 32.0 41.0 18-19 37.73257652453963 40.0 37.0 41.0 32.0 41.0 20-21 37.631832258727826 40.0 37.0 41.0 32.0 41.0 22-23 37.53783075264376 40.0 37.0 41.0 32.0 41.0 24-25 37.539865262902445 40.0 37.0 41.0 32.0 41.0 26-27 37.45026761706191 40.0 37.0 41.0 32.0 41.0 28-29 37.33484910445425 40.0 37.0 41.0 31.0 41.0 30-31 37.12462602617029 40.0 36.5 41.0 31.0 41.0 32-33 36.90668507744847 39.0 36.0 41.0 30.5 41.0 34-35 36.58097262835754 39.0 35.5 41.0 30.0 41.0 36-37 36.33441619790649 39.0 35.0 41.0 30.0 41.0 38-39 36.15509227312177 39.0 35.0 41.0 29.5 41.0 40-41 35.95997472724224 39.0 35.0 41.0 29.0 41.0 42-43 35.682765989175834 39.0 35.0 40.0 27.5 41.0 44-45 35.626740657138015 39.0 35.0 41.0 27.0 41.0 46-47 35.414429577601766 39.0 35.0 41.0 26.0 41.0 48-49 35.16443791386051 39.0 35.0 41.0 25.5 41.0 50-51 33.9834522948331 38.0 33.5 40.0 23.5 40.5 52-53 34.066071450073686 38.0 33.5 39.5 23.5 40.5 54-55 34.57644117704268 38.0 34.0 40.0 24.0 41.0 56-57 34.47250829226182 38.0 34.0 40.0 23.5 41.0 58-59 34.2403875989815 38.0 34.0 40.0 23.0 41.0 60-61 33.94109217493917 37.5 34.0 40.0 22.5 41.0 62-63 33.568058721532836 37.0 33.0 40.0 22.0 41.0 64-65 33.16607124166721 36.0 33.0 39.5 20.5 41.0 66-67 32.76652315951617 36.0 32.5 39.0 19.5 41.0 68-69 32.356784116833595 35.0 32.0 39.0 18.0 40.5 70-71 31.95098916656864 35.0 31.5 38.0 17.0 40.0 72-73 31.543597822754467 35.0 31.0 37.0 13.5 39.5 74-75 31.097433613277254 35.0 31.0 37.0 9.5 39.0 76-77 30.67094679521795 35.0 31.0 36.0 7.0 39.0 78-79 30.27154876572427 34.5 30.0 36.0 4.0 38.5 80-81 29.851685799924233 34.0 30.0 35.0 2.0 37.0 82-83 29.412688804682382 34.0 29.5 35.0 2.0 37.0 84-85 29.01640923064668 34.0 29.0 35.0 2.0 36.0 86-87 28.64502056740282 34.0 29.0 35.0 2.0 36.0 88-89 28.287992846563746 34.0 29.0 35.0 2.0 35.5 90-91 27.885780635056193 34.0 27.5 35.0 2.0 35.0 92-93 27.440684508730378 34.0 27.0 35.0 2.0 35.0 94-95 26.930538818715085 33.5 25.5 35.0 2.0 35.0 96-97 26.351277439027214 33.0 25.0 35.0 2.0 35.0 98-99 25.666231946210754 33.0 22.0 35.0 2.0 35.0 100-101 23.47595220915488 31.0 9.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36125897077240055 1101 2 0.12532433767717777 1101 3 0.25659160937717473 1101 4 0.2752641599002956 1101 5 0.06329954465796561 1101 6 0.027045442678165443 1101 7 0.18030180989077849 1101 8 0.2932485215355243 1101 9 0.37996154035515417 1101 10-11 0.4137754530443871 1101 12-13 0.2668328509971971 1101 14-15 0.48957669574789975 1101 16-17 0.4663930629055173 1101 18-19 0.4383987240979792 1101 20-21 0.5083866778725437 1101 22-23 0.48739920421601823 1101 24-25 0.5009704539942348 1101 26-27 0.5412041119911848 1101 28-29 0.3420912272515011 1101 30-31 0.37856122700527095 1101 32-33 0.3085328674834855 1101 34-35 0.2607410006353561 1101 36-37 0.4481732049830356 1101 38-39 0.47346673704835496 1101 40-41 0.33982565093882044 1101 42-43 0.38350644183924487 1101 44-45 0.5615229632799057 1101 46-47 0.5382306950807845 1101 48-49 0.4958550335534042 1101 50-51 0.3873450326240615 1101 52-53 0.40184652316811054 1101 54-55 0.4624998650730632 1101 56-57 0.3643729075806945 1101 58-59 0.3148082507119838 1101 60-61 0.2963841482289311 1101 62-63 0.2599233354252064 1101 64-65 0.21125900421907318 1101 66-67 0.012944949865591582 1101 68-69 0.12335884383073648 1101 70-71 -2.0023322804263444E-6 1101 72-73 -0.010749863579071928 1101 74-75 -0.015217409402684012 1101 76-77 -0.12641310513974346 1101 78-79 -0.16924698978537123 1101 80-81 -0.11967665228370095 1101 82-83 -0.26026225033491457 1101 84-85 -0.11512920000061655 1101 86-87 -0.21266696457705336 1101 88-89 -0.3018777550743188 1101 90-91 -0.32518484416372573 1101 92-93 -0.49291644664646483 1101 94-95 -0.59472375930007 1101 96-97 -0.7350176054887747 1101 98-99 -0.8130940093430112 1101 100-101 -0.49025818017098644 1102 1 0.2539333965878292 1102 2 0.3188753204270611 1102 3 0.34449728958831827 1102 4 0.3036482453265563 1102 5 0.32320543661975876 1102 6 0.33139138273760693 1102 7 0.31775066832790344 1102 8 0.2959207542192388 1102 9 0.41661321810091323 1102 10-11 0.2973529197503808 1102 12-13 0.4133180776082028 1102 14-15 0.3269533774639939 1102 16-17 0.450860033585613 1102 18-19 0.48494003438074174 1102 20-21 0.45337128813790883 1102 22-23 0.44737908790440883 1102 24-25 0.5264503792765041 1102 26-27 0.448454212194612 1102 28-29 0.4797446368150702 1102 30-31 0.48929032986314525 1102 32-33 0.5022061816080097 1102 34-35 0.5617321751708246 1102 36-37 0.516147129403187 1102 38-39 0.498226770625962 1102 40-41 0.4421659493559815 1102 42-43 0.46825044286774187 1102 44-45 0.6221379476632976 1102 46-47 0.5773456076202024 1102 48-49 0.5708307654165807 1102 50-51 0.6000708533468853 1102 52-53 0.6572322647869413 1102 54-55 0.551608258717522 1102 56-57 0.4507293897974236 1102 58-59 0.46294991189582646 1102 60-61 0.44210949251279885 1102 62-63 0.34526417078151184 1102 64-65 0.46324729743322735 1102 66-67 0.4147871805074175 1102 68-69 0.38084787439089496 1102 70-71 0.23061112167040676 1102 72-73 0.1764448789548254 1102 74-75 0.11182947067839066 1102 76-77 0.1652841821832034 1102 78-79 0.04827691913159882 1102 80-81 -0.07404320778127982 1102 82-83 -0.06490412705066362 1102 84-85 -0.13213292832453405 1102 86-87 -0.1707485339518584 1102 88-89 -0.2938721285570871 1102 90-91 -0.2160626262269325 1102 92-93 -0.29083799303120017 1102 94-95 -0.3179391626472423 1102 96-97 -0.3848706959138646 1102 98-99 -0.45666324802380487 1102 100-101 -0.2416863626697925 1103 1 0.17926672992116366 1103 2 0.25002683557858063 1103 3 0.23831547140650144 1103 4 0.29273915441746823 1103 5 0.24005392146824533 1103 6 0.2909065342527626 1103 7 0.14805369863093176 1103 8 0.15410257240105807 1103 9 0.25782533931303675 1103 10-11 0.27965595005341015 1103 12-13 0.3152574715475964 1103 14-15 0.44101398352459853 1103 16-17 0.4580115487371259 1103 18-19 0.48881882225953177 1103 20-21 0.4715531063197247 1103 22-23 0.4748942394195623 1103 24-25 0.3265715913977161 1103 26-27 0.3754845152249189 1103 28-29 0.3999870610574874 1103 30-31 0.4138963904692119 1103 32-33 0.3498425452443712 1103 34-35 0.34403520547385824 1103 36-37 0.31554106879712407 1103 38-39 0.253014649413835 1103 40-41 0.31501443420447117 1103 42-43 0.3012201398374401 1103 44-45 0.44116825069360033 1103 46-47 0.2929819712565731 1103 48-49 0.31228531087112543 1103 50-51 0.34431327758930763 1103 52-53 0.33189893145360116 1103 54-55 0.2516082587175248 1103 56-57 0.2598202988883358 1103 58-59 0.2994347603806702 1103 60-61 0.1673216137249227 1103 62-63 0.13653689805423852 1103 64-65 -0.004267854081923872 1103 66-67 -0.035758274038037996 1103 68-69 -0.13951576197274562 1103 70-71 -0.15932827226898283 1103 72-73 -0.039433908923960814 1103 74-75 -0.13265537780646142 1103 76-77 -0.13023096933195077 1103 78-79 -0.21475338389870302 1103 80-81 -0.22628563202370344 1103 82-83 -0.1313283694749039 1103 84-85 -0.11698141317301847 1103 86-87 -0.14505156425488863 1103 88-89 -0.2976297043146623 1103 90-91 -0.335092929257236 1103 92-93 -0.3120501142433234 1103 94-95 -0.2743027990108793 1103 96-97 -0.30281008985325997 1103 98-99 -0.19920870256925483 1103 100-101 -0.18338333236676263 1104 1 0.20641505528001503 1104 2 0.22177803175082644 1104 3 0.19034736933313567 1104 4 0.22326228041427498 1104 5 0.19510017346186004 1104 6 0.23664497125435702 1104 7 0.22628336370270574 1104 8 0.13167195039148538 1104 9 0.10976154345976852 1104 10-11 0.2025060297982293 1104 12-13 0.21775029451410433 1104 14-15 0.2947332817702062 1104 16-17 0.2813081994548341 1104 18-19 0.24751100726750508 1104 20-21 0.388785961184162 1104 22-23 0.48617494117394955 1104 24-25 0.4231617030404564 1104 26-27 0.36357223452316134 1104 28-29 0.4126936001324566 1104 30-31 0.30657103161753696 1104 32-33 0.2647309025011495 1104 34-35 0.28005274933350677 1104 36-37 0.30837998426761004 1104 38-39 0.3153750959848054 1104 40-41 0.2208421854006417 1104 42-43 0.40991232484542195 1104 44-45 0.5050167355420854 1104 46-47 0.4155944114479553 1104 48-49 0.453770159355976 1104 50-51 0.3375253988014322 1104 52-53 0.4385257257119761 1104 54-55 0.40377891262501464 1104 56-57 0.40592396714989576 1104 58-59 0.3775192898862514 1104 60-61 0.4317649949051443 1104 62-63 0.3259770894417997 1104 64-65 0.35525208212222026 1104 66-67 0.3357201948614872 1104 68-69 0.26542682175931276 1104 70-71 0.314513832994173 1104 72-73 0.13072558070921403 1104 74-75 -0.04795840810949059 1104 76-77 0.018501088084317274 1104 78-79 0.19278569106142385 1104 80-81 0.17762824357438234 1104 82-83 0.15973702127469736 1104 84-85 0.14572193610927897 1104 86-87 0.13543647402262238 1104 88-89 0.10690299106013512 1104 90-91 0.04441584267258847 1104 92-93 0.11165482993530773 1104 94-95 0.10615493623632943 1104 96-97 0.040819894197777984 1104 98-99 0.07418683172101126 1104 100-101 0.42864856555987174 1105 1 0.17427675903795503 1105 2 0.2270690010050842 1105 3 0.18268902904696205 1105 4 0.20520674850783394 1105 5 0.1865583968268112 1105 6 0.14822045639230197 1105 7 0.18021157634041174 1105 8 0.17891266622182656 1105 9 0.20803950948450023 1105 10-11 0.19101667473798045 1105 12-13 0.26284854239845856 1105 14-15 0.3140397572763476 1105 16-17 0.1739360607612852 1105 18-19 0.1889374462215727 1105 20-21 0.22089204733126877 1105 22-23 0.33203346081500484 1105 24-25 0.3244997699806973 1105 26-27 0.19263581469650148 1105 28-29 0.32236471661664723 1105 30-31 0.23464134895082367 1105 32-33 0.343570572851398 1105 34-35 0.25986524968823943 1105 36-37 0.34652780448136156 1105 38-39 0.3559910324613895 1105 40-41 0.3269518870244852 1105 42-43 0.3272311395139198 1105 44-45 0.28846599683831897 1105 46-47 0.2547065479847035 1105 48-49 0.23903652407074105 1105 50-51 0.26832449294572314 1105 52-53 0.23548137510722 1105 54-55 0.3374018530235716 1105 56-57 0.26417358261528534 1105 58-59 0.23917551310362484 1105 60-61 0.3752094601608107 1105 62-63 0.1796077489115646 1105 64-65 0.16331070733293984 1105 66-67 0.16319654101642556 1105 68-69 0.12779535632769523 1105 70-71 0.09077158753905934 1105 72-73 0.12870585167131665 1105 74-75 0.1348222454007022 1105 76-77 0.15924600683541712 1105 78-79 0.05184297111046021 1105 80-81 0.042925626468694134 1105 82-83 0.11765815052941164 1105 84-85 0.08485833663825915 1105 86-87 0.07404516819415718 1105 88-89 -0.052594872005808924 1105 90-91 -0.0381935439450416 1105 92-93 0.13985455522718482 1105 94-95 0.12388850908790161 1105 96-97 0.15827521155944524 1105 98-99 0.2904015637954558 1105 100-101 0.35337456691070557 1106 1 0.06783946582723388 1106 2 0.082479740698151 1106 3 0.09909817018920108 1106 4 0.1588318765101846 1106 5 0.09745321353420167 1106 6 0.06556439491061639 1106 7 0.11365846423569792 1106 8 0.099992583624406 1106 9 0.012751178905546112 1106 10-11 0.10675454281866337 1106 12-13 0.16229209124888655 1106 14-15 0.24296321947383603 1106 16-17 0.1595787593043383 1106 18-19 0.2650059067799475 1106 20-21 0.16526112669441773 1106 22-23 0.13669316888515937 1106 24-25 0.1755764260692203 1106 26-27 0.03179971854012109 1106 28-29 0.12141656982033311 1106 30-31 0.1068432090826903 1106 32-33 0.10969016675866072 1106 34-35 0.11164351074882717 1106 36-37 0.21314445306716578 1106 38-39 0.18652064958649817 1106 40-41 0.17786007005010163 1106 42-43 0.1580059466232484 1106 44-45 0.16475514528049473 1106 46-47 0.11053027480487287 1106 48-49 0.08956399514980973 1106 50-51 0.17061915689518514 1106 52-53 0.06396920685721597 1106 54-55 0.04014482092074445 1106 56-57 0.09273517413654275 1106 58-59 0.10103503664760893 1106 60-61 0.17973195646859352 1106 62-63 -0.04821092202691801 1106 64-65 -0.16868329049735564 1106 66-67 -0.282571016850774 1106 68-69 -0.03017914796946286 1106 70-71 -0.10040174200911878 1106 72-73 -0.1557953869521036 1106 74-75 -0.14690729872504704 1106 76-77 -0.24421309831407711 1106 78-79 0.01293659279127013 1106 80-81 -0.05362388636195803 1106 82-83 0.03485845604525295 1106 84-85 -0.08027373813201066 1106 86-87 0.08718006831007585 1106 88-89 0.17885678450516096 1106 90-91 0.19429134079370058 1106 92-93 0.22530005410684595 1106 94-95 0.3070157713076931 1106 96-97 0.3184645367546999 1106 98-99 0.4018835015229456 1106 100-101 0.686046090062657 1107 1 0.02896154574363763 1107 2 0.08706571159617837 1107 3 0.05754299565530374 1107 4 0.13284446754405366 1107 5 0.0021054302431551264 1107 6 0.0366262329937328 1107 7 -0.04113158417136731 1107 8 0.00476459123328965 1107 9 0.013695795098385588 1107 10-11 0.0384993477904203 1107 12-13 0.0825597012328032 1107 14-15 0.1287628020819227 1107 16-17 0.0788790216897084 1107 18-19 0.15376309784210207 1107 20-21 0.22686828759236022 1107 22-23 0.1647780010479991 1107 24-25 0.09091998059717099 1107 26-27 0.1113780401397193 1107 28-29 0.0014546431208160016 1107 30-31 0.10637523077783584 1107 32-33 0.19556689181978015 1107 34-35 0.2518937582685794 1107 36-37 0.3126849178558615 1107 38-39 0.27650959418537013 1107 40-41 0.30247471860091935 1107 42-43 0.3752510734428398 1107 44-45 0.37741827017766383 1107 46-47 0.39742875851186454 1107 48-49 0.3853114903653889 1107 50-51 0.4590081713480316 1107 52-53 0.4338582487320721 1107 54-55 0.2983472502224629 1107 56-57 0.2402948314479687 1107 58-59 0.30004813282382514 1107 60-61 0.21838436657490945 1107 62-63 0.26408900552123526 1107 64-65 0.21554674266884177 1107 66-67 0.29202696200251665 1107 68-69 0.19354190376576952 1107 70-71 0.22796026897700017 1107 72-73 0.12155843206837957 1107 74-75 0.1495116749988945 1107 76-77 0.14595199663824943 1107 78-79 0.1394563496301764 1107 80-81 0.0814905067311571 1107 82-83 0.22670213902368985 1107 84-85 0.19227401225262142 1107 86-87 0.21794662904117956 1107 88-89 0.2189575098895702 1107 90-91 0.2966569945777948 1107 92-93 0.4243546441188819 1107 94-95 0.49555979229851843 1107 96-97 0.49472746479068164 1107 98-99 0.5222744384032474 1107 100-101 0.4355449166891425 1108 1 0.10127752278127033 1108 2 0.03313019796961214 1108 3 -0.037936915476020516 1108 4 -0.02100264715995337 1108 5 0.11763299992403375 1108 6 0.1057782652614776 1108 7 0.02671704441756617 1108 8 0.110562514285661 1108 9 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2316 92-93 -2.211248747243996 2316 94-95 -2.2594331184015495 2316 96-97 -2.3562156269276606 2316 98-99 -2.5966657202566275 2316 100-101 -3.0634747322686025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11517.0 3 3352.0 4 3536.0 5 4130.0 6 4842.0 7 5929.0 8 7385.0 9 9236.0 10 11658.0 11 15261.0 12 19922.0 13 25963.0 14 27595.0 15 26897.0 16 33984.0 17 42714.0 18 36537.0 19 26424.0 20 27520.0 21 30135.0 22 33346.0 23 36972.0 24 42439.0 25 49061.0 26 57026.0 27 67129.0 28 78677.0 29 93309.0 30 112086.0 31 134952.0 32 166634.0 33 210487.0 34 278802.0 35 393821.0 36 574967.0 37 816680.0 38 688801.0 39 108746.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.482500448801595 18.578623313282996 13.782012774261842 23.156863463653565 2 15.728114069377 20.561849812269394 39.485451949934486 24.22458416841912 3 19.06727494210506 25.194497338408684 29.733293902350837 26.004933817135417 4 12.961063056263578 16.185595352119076 36.76543537259171 34.08790621902564 5 13.836109070194421 37.5464422861348 33.56893072787423 15.048517915796555 6 31.96122054604058 35.54179440338286 17.494073186246734 15.002911864329823 7 28.589286181740764 32.92399935413432 20.930820325865646 17.55589413825927 8 26.728653903180373 33.9176365927793 20.02504355400697 19.32866595003336 9 27.490905232558006 16.031625673897768 20.27504735904819 36.202421734496035 10-11 25.365536728464665 26.003150422784238 27.044928216624175 21.586384632126922 12-13 26.368131195380194 23.769814530561142 27.604783323214882 22.257270950843782 14-15 23.53098439723673 24.75884858932477 26.145997698587202 25.56416931485129 16-17 22.576645160587848 28.049870596334113 26.666931539825434 22.7065527032526 18-19 22.49350837702012 26.44611211556479 29.205445734943464 21.854933772471625 20-21 23.38526152977522 25.615867575743152 28.982135759792328 22.016735134689302 22-23 23.13667748149885 25.599592926070947 28.89609697479747 22.367632617632733 24-25 22.874992525943664 25.754091727181418 29.015063062495162 22.355852684379755 26-27 22.584643366762933 25.900444952870778 29.091259256718498 22.423652423647788 28-29 22.463027577850127 26.153387322489618 28.988965105875337 22.39461999378492 30-31 22.698260104132853 25.987845220146376 28.863269269223345 22.450625406497423 32-33 22.44162610426103 26.179198045359076 28.78553267312924 22.593643177250655 34-35 22.864425739146917 26.03964915625849 28.716172160217234 22.37975294437736 36-37 22.91424516669381 25.86245725054091 28.634692785519857 22.588604797245424 38-39 22.697858138124964 26.127565506125627 28.678531384430418 22.49604497131899 40-41 22.740061329906013 25.993297288196842 28.732795873079137 22.533845508818004 42-43 22.61541433577027 26.212993657775268 28.21905616912227 22.95253583733219 44-45 22.64332989386021 26.131318548833132 28.28468229422744 22.940669263079215 46-47 22.803440746251074 26.300320762712015 27.937051553617692 22.959186937419226 48-49 22.947341088636893 26.43215551929031 27.602838275443105 23.017665116629697 50-51 22.617446939831197 26.673469200214917 27.651298501058008 23.057785358895877 52-53 22.86380189535804 26.65471573991728 27.447815999157775 23.03366636556691 54-55 22.478362199243644 26.466152347239593 27.662663871270322 23.39282158224644 56-57 22.56256742153982 26.668716545077242 27.45599312654991 23.312722906833024 58-59 22.765608513285294 26.34642048443872 27.551221270423543 23.33674973185244 60-61 22.67322429701774 26.520987094387266 27.76866952734825 23.037119081246743 62-63 22.593576124118965 26.330573358561672 27.941839907972156 23.134010609347207 64-65 22.378178064698677 26.337786533084635 27.907540608863023 23.37649479335366 66-67 22.458069281801162 26.477698671715448 27.471489182579177 23.592742863904206 68-69 22.39308291141774 26.330442059562476 27.77289072455916 23.503584304460624 70-71 22.40385733070509 26.240523265494474 27.660481547325872 23.69513785647456 72-73 22.331313168449316 26.245987137198984 27.53170878584992 23.890990908501784 74-75 22.168336425752678 26.57675867995711 27.495701073704904 23.75920382058531 76-77 22.237060181203 26.137776206745382 27.67852396776251 23.946639644289107 78-79 22.12379985110863 26.234662293108553 27.69473733417462 23.946800521608203 80-81 22.276341945998173 26.418547554483396 27.443801297865505 23.86130920165293 82-83 22.30767602486992 26.102969312229867 27.572284682353537 24.01706998054668 84-85 22.12434676954987 26.007611567451853 27.5125726472277 24.355469015770574 86-87 22.099044459263 25.874378920620828 27.757928594350968 24.268648025765206 88-89 22.09540622847562 25.789005738128374 27.63482251842263 24.480765514973378 90-91 22.12167830475696 25.77704378546453 27.724353157640515 24.37692475213799 92-93 22.244017106495857 25.694062878855124 27.612779219244192 24.449140795404826 94-95 22.131345511776875 25.418182689683306 27.74864852224549 24.701823276294338 96-97 22.050785119750728 25.185481901589856 27.89768098032032 24.866051998339096 98-99 22.040212334528093 25.120412477154723 27.780608594592522 25.058766593724663 100-101 22.250816126294996 24.737155861656635 28.08981954783143 24.92220846421694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4741.0 1 2893.5 2 1418.0 3 1714.5 4 1418.5 5 1457.5 6 2543.0 7 4740.5 8 7225.5 9 8027.0 10 6751.5 11 5875.0 12 6155.5 13 6533.5 14 6967.5 15 7310.0 16 7669.0 17 8203.5 18 8524.0 19 8937.0 20 9596.5 21 10505.0 22 11554.0 23 12658.5 24 14317.0 25 17031.5 26 20995.5 27 25431.5 28 29858.5 29 35047.5 30 41233.0 31 49009.0 32 57750.0 33 64798.5 34 70646.5 35 76609.0 36 85702.0 37 93549.0 38 98022.5 39 102971.5 40 107455.5 41 114324.5 42 120327.5 43 127709.0 44 137077.0 45 142907.0 46 147443.0 47 154659.5 48 170648.0 49 176617.0 50 168415.5 51 164667.5 52 162554.5 53 161420.0 54 158266.5 55 150737.0 56 140717.5 57 128318.5 58 118632.5 59 107389.5 60 92048.0 61 76848.5 62 64173.0 63 50035.5 64 36984.5 65 28422.5 66 20349.5 67 15522.0 68 11418.0 69 7628.0 70 5722.5 71 4072.0 72 2848.0 73 2184.0 74 1707.5 75 1338.5 76 944.5 77 569.5 78 354.0 79 233.0 80 162.0 81 129.5 82 113.0 83 72.5 84 58.5 85 48.0 86 34.5 87 27.5 88 19.5 89 19.0 90 13.0 91 12.0 92 14.5 93 8.0 94 7.0 95 8.5 96 8.5 97 8.5 98 4.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16214023254456889 2 0.09394500477482376 3 0.0873454666035581 4 0.10262860763175226 5 0.12353872331123607 6 0.0384857278619071 7 0.041611824890401355 8 0.046405173667425886 9 0.04610414210171903 10-11 0.04447162476461647 12-13 0.04891762942736386 14-15 0.046428329941711026 16-17 0.04521262554174104 18-19 0.04983230226162699 20-21 0.05176585116443641 22-23 0.0649765056441103 24-25 0.08239002390653757 26-27 0.08440461976934498 28-29 0.07347485830675765 30-31 0.061387283129913185 32-33 0.050770131370175275 34-35 0.2939110113641732 36-37 0.4673862401713194 38-39 0.444843607154733 40-41 0.4199390341610621 42-43 0.4501695502403158 44-45 0.45989518544007574 46-47 0.4590384032915254 48-49 0.47521306087969756 50-51 0.4651053471542328 52-53 0.4712649161140807 54-55 0.4628476104114314 56-57 0.4409417749376865 58-59 0.4202053313153412 60-61 0.3896853618075232 62-63 0.448085485554653 64-65 0.4839661325594815 66-67 0.48508921186231097 68-69 0.49591477009061513 70-71 0.49358756452495833 72-73 0.4839082418737687 74-75 0.4772160786053624 76-77 0.45906155956581063 78-79 0.43313811050359335 80-81 0.43911242926916016 82-83 0.45096844170315326 84-85 0.4485254547660707 86-87 0.45892262192009975 88-89 0.4701997274969642 90-91 0.47535199852540844 92-93 0.47666032802251895 94-95 0.4812105359195495 96-97 0.48228730267380865 98-99 0.47583828028539643 100-101 0.46258131325715224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4318484.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.08494091343394 #Duplication Level Percentage of deduplicated Percentage of total 1 62.58020752027783 18.827218455979928 2 12.99536076116023 7.819293212965269 3 6.526375641939065 5.890368766998337 4 3.999488768670293 4.8129753315755375 5 2.734202613166085 4.112916203122917 6 1.9213847887768027 3.4682848705392524 7 1.4101776843972549 2.9697578618774156 8 1.1415364273431543 2.7474444773721016 9 0.9023099954921059 2.443134860998277 >10 5.316243306871783 28.505354132629712 >50 0.31079634102366666 6.410877163386405 >100 0.1517967442473804 8.164472881569429 >500 0.007492995819717594 1.4835609220295785 >1k 0.0023174213071369787 1.182883990047139 >5k 1.5449475380913163E-4 0.3678399928184462 >10k+ 1.5449475380913163E-4 0.7936168760902907 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20055 0.464399080788536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14094 0.32636452977480057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9995 0.23144696148000085 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5833 0.13507054790523712 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.3156274285142657E-5 0.0 10-11 0.0 0.0 0.0 3.4734411427713983E-5 0.0 12-13 0.0 0.0 0.0 6.946882285542797E-5 0.0 14-15 0.0 0.0 0.0 8.104695999799929E-5 0.0 16-17 0.0 0.0 0.0 1.6209391999599858E-4 0.0 18-19 0.0 0.0 0.0 2.3156274285142657E-4 0.0 20-21 0.0 0.0 0.0 3.473441142771398E-4 0.0 22-23 0.0 0.0 0.0 0.0010420323428314195 2.3156274285142657E-5 24-25 0.0 0.0 0.0 0.0018525019428114126 2.3156274285142657E-5 26-27 0.0 0.0 0.0 0.003855519668476252 2.3156274285142657E-5 28-29 0.0 0.0 0.0 0.010952917736872476 2.3156274285142657E-5 30-31 0.0 0.0 0.0 0.03259245605633829 2.3156274285142657E-5 32-33 1.1578137142571328E-5 0.0 0.0 0.07129816852395424 2.3156274285142657E-5 34-35 2.3156274285142657E-5 0.0 0.0 0.1307982152996283 2.3156274285142657E-5 36-37 2.3156274285142657E-5 0.0 0.0 0.22720936328581975 2.3156274285142657E-5 38-39 2.3156274285142657E-5 0.0 0.0 0.3723413123679513 2.3156274285142657E-5 40-41 2.3156274285142657E-5 0.0 0.0 0.5424588814037519 2.3156274285142657E-5 42-43 2.3156274285142657E-5 0.0 0.0 0.7054558960968711 2.3156274285142657E-5 44-45 2.3156274285142657E-5 0.0 0.0 0.9018326801720233 2.3156274285142657E-5 46-47 2.3156274285142657E-5 0.0 0.0 1.1381540373890466 2.3156274285142657E-5 48-49 2.3156274285142657E-5 0.0 0.0 1.3908700367999511 2.3156274285142657E-5 50-51 2.3156274285142657E-5 0.0 0.0 1.6640214482674938 2.3156274285142657E-5 52-53 2.3156274285142657E-5 0.0 0.0 1.9342667473122512 2.3156274285142657E-5 54-55 2.3156274285142657E-5 0.0 0.0 2.2221455492251447 2.3156274285142657E-5 56-57 2.3156274285142657E-5 0.0 0.0 2.5591619651711106 2.3156274285142657E-5 58-59 2.3156274285142657E-5 0.0 0.0 2.929453947264827 2.3156274285142657E-5 60-61 2.3156274285142657E-5 0.0 0.0 3.334711903529109 2.3156274285142657E-5 62-63 2.3156274285142657E-5 0.0 0.0 3.742030768204768 2.3156274285142657E-5 64-65 2.3156274285142657E-5 0.0 0.0 4.155624983211701 4.6312548570285314E-5 66-67 2.3156274285142657E-5 0.0 0.0 4.612035149371863 4.6312548570285314E-5 68-69 2.3156274285142657E-5 0.0 0.0 5.124819265279205 4.6312548570285314E-5 70-71 2.3156274285142657E-5 0.0 0.0 5.683012835059711 4.6312548570285314E-5 72-73 2.3156274285142657E-5 0.0 0.0 6.253120307959923 4.6312548570285314E-5 74-75 2.3156274285142657E-5 0.0 0.0 6.8251613297629445 4.6312548570285314E-5 76-77 2.3156274285142657E-5 0.0 0.0 7.425962444228114 4.6312548570285314E-5 78-79 2.3156274285142657E-5 0.0 0.0 8.102936122954258 4.6312548570285314E-5 80-81 2.3156274285142657E-5 0.0 0.0 8.8280748521935 4.6312548570285314E-5 82-83 2.3156274285142657E-5 0.0 0.0 9.562626606929655 4.6312548570285314E-5 84-85 2.3156274285142657E-5 0.0 0.0 10.30708924705985 4.6312548570285314E-5 86-87 2.3156274285142657E-5 0.0 0.0 11.059343973486992 4.6312548570285314E-5 88-89 2.3156274285142657E-5 0.0 0.0 11.855942965170184 4.6312548570285314E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 27020 0.0 29.721916 1 GGTATCA 18550 0.0 28.802404 1 GTACATG 22190 0.0 28.500158 1 TACATGG 22890 0.0 27.26101 2 ACATGGG 22950 0.0 25.352316 3 ATCAACG 32780 0.0 24.369722 3 TCAACGC 32735 0.0 24.330853 4 TATCAAC 33005 0.0 24.232304 2 CAACGCA 32965 0.0 24.117975 5 AACGCAG 33420 0.0 23.716413 6 CATGGGA 12455 0.0 22.78689 4 GAGTACT 15615 0.0 22.181707 12-13 AGTACTT 16255 0.0 22.022036 12-13 CATGGGG 10655 0.0 21.522558 4 ACGCAGA 36800 0.0 21.294394 7 CGCAGAG 36850 0.0 21.111065 8 AGAGTAC 28845 0.0 19.837465 10-11 GCAGAGT 38295 0.0 19.733353 9 GTACTTT 17575 0.0 19.641172 14-15 TACTTTT 19585 0.0 18.229845 14-15 >>END_MODULE