FastQCFastQC Report
Thu 26 May 2016
SRR938835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318484
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187150.4333696732464448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145780.33757216652880967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102240.23674974829129852No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60860.1409290852993782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA259650.029.8482721
GGTATCA178700.028.921791
GTACATG212000.026.7156641
TACATGG217950.025.6904952
ATCAACG314950.024.3922883
TCAACGC315150.024.3918674
ACATGGG219550.024.2929463
CAACGCA317050.024.2307285
TATCAAC319300.024.1640072
AACGCAG320800.023.9474816
GAGTACT162100.022.1466712-13
CATGGGG107400.021.295614
CATGGGA114400.021.1124734
ACGCAGA361300.021.0923487
AGTACTT168600.020.98324212-13
CGCAGAG361950.020.897158
GTACTTT176300.020.18791414-15
AGAGTAC289200.019.69078310-11
GCAGAGT385100.019.38249
ATGGGGA50950.018.4403155
Produced by FastQC (version 0.11.5)