##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938835_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4318484 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.003176577706434 33.0 31.0 34.0 30.0 34.0 2 32.21645489481957 34.0 31.0 34.0 30.0 34.0 3 32.30432299853374 34.0 31.0 34.0 30.0 34.0 4 35.946106087228756 37.0 35.0 37.0 35.0 37.0 5 35.85141776604938 37.0 35.0 37.0 35.0 37.0 6 35.88030290259267 37.0 35.0 37.0 35.0 37.0 7 35.80645545983266 37.0 35.0 37.0 33.0 37.0 8 35.775926690940615 37.0 35.0 37.0 33.0 37.0 9 37.42161994811142 39.0 37.0 39.0 34.0 39.0 10-11 37.34931864978543 39.0 37.0 39.0 34.0 39.0 12-13 37.274472476915506 39.0 37.0 39.0 33.0 39.0 14-15 38.49565321997257 40.0 38.0 41.0 34.0 41.0 16-17 38.43294626077114 40.0 38.0 41.0 33.0 41.0 18-19 38.45181214055673 40.0 38.0 41.0 33.5 41.0 20-21 38.51365039212835 40.0 38.0 41.0 34.0 41.0 22-23 38.46451092559333 40.0 38.0 41.0 34.0 41.0 24-25 38.38642310588623 40.0 38.0 41.0 34.0 41.0 26-27 38.22113859863785 40.0 38.0 41.0 33.0 41.0 28-29 38.05842733236941 40.0 37.0 41.0 33.0 41.0 30-31 37.82539370297539 40.0 37.0 41.0 33.0 41.0 32-33 37.59524465066908 40.0 36.5 41.0 32.0 41.0 34-35 37.56714601698189 40.0 37.0 41.0 32.0 41.0 36-37 37.47878885275481 40.0 36.5 41.0 32.0 41.0 38-39 37.37593574504386 40.0 36.0 41.0 32.0 41.0 40-41 37.20435655197518 40.0 36.0 41.0 31.0 41.0 42-43 37.00883168723098 40.0 36.0 41.0 31.0 41.0 44-45 36.73785974429916 39.5 35.5 41.0 30.5 41.0 46-47 36.410766139228485 39.0 35.0 41.0 30.0 41.0 48-49 36.109728205546205 39.0 35.0 41.0 29.5 41.0 50-51 35.851119744799334 39.0 35.0 41.0 28.5 41.0 52-53 35.62752646530588 39.0 35.0 40.5 28.0 41.0 54-55 35.36959370464265 38.0 35.0 40.0 28.0 41.0 56-57 35.095383240970676 38.0 34.0 40.0 27.0 41.0 58-59 34.77982180783812 38.0 34.0 40.0 26.0 41.0 60-61 34.41725267478124 37.0 34.0 40.0 26.0 41.0 62-63 34.037366006218846 37.0 33.0 40.0 26.0 41.0 64-65 33.66538523704152 36.0 33.0 39.0 25.0 41.0 66-67 33.27091879928234 36.0 33.0 39.0 24.5 40.0 68-69 32.82210099655342 35.0 32.0 38.5 23.0 40.0 70-71 32.35331750679174 35.0 32.0 37.5 22.0 40.0 72-73 32.02676795838539 35.0 31.5 37.0 21.5 39.0 74-75 31.62122934807678 35.0 31.0 36.5 20.0 39.0 76-77 29.59109990913478 33.0 28.5 35.0 17.5 36.5 78-79 30.66022347194062 34.0 30.5 35.0 18.0 37.0 80-81 30.729063717730575 34.0 31.0 35.0 18.0 37.0 82-83 30.441631947692755 34.0 31.0 35.0 17.0 37.0 84-85 30.082603293192705 34.0 30.5 35.0 11.5 36.0 86-87 29.580305959220876 34.0 30.0 35.0 6.5 36.0 88-89 29.097226711966513 34.0 29.0 35.0 2.0 35.5 90-91 28.71078797559514 34.0 29.0 35.0 2.0 35.0 92-93 28.44586572510168 34.0 29.0 35.0 2.0 35.0 94-95 28.079844454674372 34.0 29.0 35.0 2.0 35.0 96-97 27.59049321474851 33.0 27.5 35.0 2.0 35.0 98-99 26.891052276678575 33.0 26.5 35.0 2.0 35.0 100-101 23.885256955913235 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06700239828108323 1101 2 0.14228195650249376 1101 3 0.10337291744871635 1101 4 0.08668515648182051 1101 5 0.08853319567198525 1101 6 0.15200479841139014 1101 7 0.10335087472931548 1101 8 0.15229944994482736 1101 9 0.2097490052073141 1101 10-11 0.18357560303296339 1101 12-13 0.20087900290401706 1101 14-15 0.2634208601889867 1101 16-17 0.20905162308850578 1101 18-19 0.22565146031560346 1101 20-21 0.18098269888846374 1101 22-23 0.2021088105074682 1101 24-25 0.2025031507282904 1101 26-27 0.26140081643217883 1101 28-29 0.20144317122316835 1101 30-31 0.22072514096164042 1101 32-33 0.25430132889157164 1101 34-35 0.14118917602841918 1101 36-37 0.20569954883038832 1101 38-39 0.19686307538770365 1101 40-41 0.27462392155282345 1101 42-43 0.2840536498792616 1101 44-45 0.3079236032170414 1101 46-47 0.33554583495618573 1101 48-49 0.23859338567448418 1101 50-51 0.3086726968437361 1101 52-53 0.23840113115287664 1101 54-55 0.12288268984924144 1101 56-57 0.10479426812924686 1101 58-59 0.17624391675807516 1101 60-61 0.21465519342950756 1101 62-63 0.17751944678936127 1101 64-65 0.2251937503317194 1101 66-67 0.11033989399465582 1101 68-69 0.07723592262667722 1101 70-71 -0.02486910315774793 1101 72-73 0.05072706039727848 1101 74-75 0.08995272387688757 1101 76-77 0.05420486308250361 1101 78-79 0.09154664978878202 1101 80-81 0.12286109057052741 1101 82-83 0.1348451294960924 1101 84-85 0.15025689304236423 1101 86-87 0.2653994082377622 1101 88-89 0.32539620700366356 1101 90-91 0.3837568226052035 1101 92-93 0.38396572309392596 1101 94-95 0.341921462053584 1101 96-97 0.36521785670428386 1101 98-99 0.24703777372591063 1101 100-101 0.786294145006508 1102 1 0.07398684217094598 1102 2 0.1050420959554188 1102 3 0.14528290829806423 1102 4 0.09988847491698039 1102 5 0.0634439503361719 1102 6 0.08404596878747128 1102 7 0.06716813391967236 1102 8 0.06039422348507628 1102 9 0.1925207304457217 1102 10-11 0.22492481766493455 1102 12-13 0.14813403387254453 1102 14-15 0.19912189852342266 1102 16-17 0.20943047524874459 1102 18-19 0.17788662453580173 1102 20-21 0.23209779292844246 1102 22-23 0.12621235657456964 1102 24-25 0.08304444588601001 1102 26-27 0.18264103998036774 1102 28-29 0.07829891609387829 1102 30-31 0.16218053341878402 1102 32-33 0.24669775635562985 1102 34-35 0.3004495612633491 1102 36-37 0.23085337811398432 1102 38-39 0.22760031552049753 1102 40-41 0.28013808993996747 1102 42-43 0.2765440946312552 1102 44-45 0.22484341778396555 1102 46-47 0.1960092361253558 1102 48-49 0.24882880702046606 1102 50-51 0.2787009050551177 1102 52-53 0.315241781227364 1102 54-55 0.37864574087358704 1102 56-57 0.2438297741754809 1102 58-59 0.25502465652409256 1102 60-61 0.17742616241559972 1102 62-63 0.10484347821920892 1102 64-65 0.15114627281132442 1102 66-67 0.18750863657392358 1102 68-69 0.28609054824396907 1102 70-71 0.28037641415665604 1102 72-73 0.1386984438096448 1102 74-75 0.23505270799558886 1102 76-77 0.0875743755266356 1102 78-79 0.1002703227550299 1102 80-81 0.15369528225023998 1102 82-83 0.25499036019085963 1102 84-85 0.306317998229499 1102 86-87 0.23097522631370637 1102 88-89 0.38243091142244623 1102 90-91 0.48549505342853294 1102 92-93 0.404604574800409 1102 94-95 0.4046745532349263 1102 96-97 0.36572723801426577 1102 98-99 0.4069347419103764 1102 100-101 0.6544744053085552 1103 1 -6.79824495719572E-4 1103 2 0.04540573231905398 1103 3 -0.04865648564133096 1103 4 -0.008717585689076657 1103 5 0.09544395033616837 1103 6 0.03798536272686448 1103 7 0.07977419452573287 1103 8 0.09409119318204517 1103 9 0.10888436680935598 1103 10-11 0.004076332816445927 1103 12-13 0.10025524599375757 1103 14-15 0.07475826215978287 1103 16-17 -0.018205888387619495 1103 18-19 0.03982601847519618 1103 20-21 0.07852203535268387 1103 22-23 -0.04215127978905997 1103 24-25 0.09601414285570797 1103 26-27 0.017307706647031296 1103 28-29 0.1258140676090349 1103 30-31 -0.0014558302175800009 1103 32-33 0.03469775635562655 1103 34-35 -0.0023383175245328403 1103 36-37 -0.08684359158298349 1103 38-39 0.06808516400534614 1103 40-41 0.025471423273302207 1103 42-43 0.10975621584337603 1103 44-45 0.03175250869306723 1103 46-47 0.12219105430717292 1103 48-49 0.10422274641440055 1103 50-51 0.20124635960057446 1103 52-53 0.12748420546979133 1103 54-55 0.07076695299479496 1103 56-57 0.0531631075088157 1103 58-59 -0.09709655559712616 1103 60-61 0.018274647264085786 1103 62-63 -0.006308036932310301 1103 64-65 -0.020611302946257126 1103 66-67 -0.011521666456374646 1103 68-69 -0.06809126993784531 1103 70-71 -0.12847207069183497 1103 72-73 -0.08324095012975263 1103 74-75 0.041113314056197225 1103 76-77 -0.06430441235215412 1103 78-79 -0.14433573785102993 1103 80-81 -0.13454714199218287 1103 82-83 -0.06658539738489822 1103 84-85 -0.15937897146747204 1103 86-87 0.009520680859157693 1103 88-89 0.22182485081638603 1103 90-91 0.23979808373156075 1103 92-93 0.23866518086101607 1103 94-95 0.21631091687128645 1103 96-97 0.2900908743779027 1103 98-99 0.48366201463764824 1103 100-101 0.84150470833886 1104 1 0.007208532761055153 1104 2 0.06658595560453762 1104 3 -0.0017665334882224215 1104 4 -0.07410833529035443 1104 5 0.05170870312723963 1104 6 0.07118791456100126 1104 7 0.05139460919878047 1104 8 -0.008680736642517672 1104 9 0.02314911960042565 1104 10-11 0.04818478576700613 1104 12-13 0.03117231138451615 1104 14-15 -0.00881749541597543 1104 16-17 0.08429969374953572 1104 18-19 0.03384994192016677 1104 20-21 0.12898774508155242 1104 22-23 0.07894441399083973 1104 24-25 0.05509069787963483 1104 26-27 0.11570005114463555 1104 28-29 0.14049349344634976 1104 30-31 0.05943571683186377 1104 32-33 0.10471370531894308 1104 34-35 0.0937988435599948 1104 36-37 0.039390858177782206 1104 38-39 0.1477406663976879 1104 40-41 0.15231033874379563 1104 42-43 0.10645956512566812 1104 44-45 0.07845904776802826 1104 46-47 0.11277638126092171 1104 48-49 0.07362147049733636 1104 50-51 0.16869293057346368 1104 52-53 0.09471227564522877 1104 54-55 0.06629645857693589 1104 56-57 0.07538001340993361 1104 58-59 0.07683167406794666 1104 60-61 0.21126826767875428 1104 62-63 0.02987856593851035 1104 64-65 0.3343583940234467 1104 66-67 0.19596158713213896 1104 68-69 0.1913505163460414 1104 70-71 0.023614053710083738 1104 72-73 -0.06680075874219682 1104 74-75 -0.04293293793742592 1104 76-77 -0.025468686674322782 1104 78-79 0.10456856836906425 1104 80-81 0.11715301749744711 1104 82-83 0.02614984982403712 1104 84-85 0.1245444735086032 1104 86-87 0.2586036154684095 1104 88-89 0.4566302734639116 1104 90-91 0.4997438572562807 1104 92-93 0.46296661626771396 1104 94-95 0.4252200077803785 1104 96-97 0.4015693432774228 1104 98-99 0.44670826663126917 1104 100-101 0.7841027944632621 1105 1 -0.035479349999871346 1105 2 0.010219708378578218 1105 3 -0.01936715101391684 1105 4 -0.011715536729731468 1105 5 0.02796546440928438 1105 6 -0.019382048035559762 1105 7 -0.022528835777301026 1105 8 0.09041255627920464 1105 9 0.035645501285792136 1105 10-11 0.0694968008418968 1105 12-13 -0.014482163690267669 1105 14-15 0.07078457511135383 1105 16-17 -0.004358805458686277 1105 18-19 -0.0625710482777464 1105 20-21 0.012751303119316049 1105 22-23 -0.0213006381412697 1105 24-25 -0.0921036183866093 1105 26-27 -0.06539390016845203 1105 28-29 0.02798121955554933 1105 30-31 -0.0822068277372594 1105 32-33 0.005289797766181437 1105 34-35 0.085505098845573 1105 36-37 -0.026660695001503143 1105 38-39 -0.005638981362928064 1105 40-41 0.055350858191879126 1105 42-43 -0.05885243292186004 1105 44-45 -0.04964078366108282 1105 46-47 0.0988359373008123 1105 48-49 0.05034719373458785 1105 50-51 0.005962848331300563 1105 52-53 -0.019323947231598027 1105 54-55 -0.03622884124654746 1105 56-57 -0.04593966397829519 1105 58-59 -0.003365077111197934 1105 60-61 0.03215019994390644 1105 62-63 -0.11080108125452881 1105 64-65 -0.12102282025457356 1105 66-67 -0.1480677680416278 1105 68-69 -0.0623779085661198 1105 70-71 -0.09973358260814535 1105 72-73 -0.22088874480245835 1105 74-75 -0.10721279399944805 1105 76-77 -0.13286906241146568 1105 78-79 -0.21725582843660263 1105 80-81 -0.16655576918942572 1105 82-83 -0.19428085732234734 1105 84-85 -0.14452078102209143 1105 86-87 -0.12009217366472669 1105 88-89 -0.03619822693622865 1105 90-91 0.12143983936620018 1105 92-93 0.13898740667790221 1105 94-95 0.06458698718294542 1105 96-97 0.014810813987519822 1105 98-99 0.14928479043836163 1105 100-101 0.5556442532542043 1106 1 -0.07680138195061303 1106 2 -0.09834200142867644 1106 3 -0.11435621800772822 1106 4 -0.0708690145071742 1106 5 -0.07301309878754836 1106 6 -0.12607213533063089 1106 7 -0.05685932010778316 1106 8 -0.08797321154902704 1106 9 -0.09055653796487917 1106 10-11 -0.0910394402993191 1106 12-13 -0.04372170298318423 1106 14-15 0.04141128814613637 1106 16-17 -0.10410254728427759 1106 18-19 -0.19772150807233402 1106 20-21 -0.13963232880168164 1106 22-23 -0.19665287695733014 1106 24-25 -0.11573462689305813 1106 26-27 -0.12687186686587637 1106 28-29 -0.12637094490931133 1106 30-31 -0.06610523020031422 1106 32-33 0.020577925569128297 1106 34-35 -0.021552942405818953 1106 36-37 0.006579614840227066 1106 38-39 0.09271185829870632 1106 40-41 0.017227617696157438 1106 42-43 0.12289029164411858 1106 44-45 0.22344205371594228 1106 46-47 0.17663618941897852 1106 48-49 0.16540525526358607 1106 50-51 0.2199272712814917 1106 52-53 0.2277050463573005 1106 54-55 0.23660162449613864 1106 56-57 0.0991956552080282 1106 58-59 0.15854421104364036 1106 60-61 0.22290885256496296 1106 62-63 0.11591254495494496 1106 64-65 0.10256611223116607 1106 66-67 -0.01476409069879736 1106 68-69 0.11945124927134287 1106 70-71 0.1583065704201445 1106 72-73 0.11529198606984892 1106 74-75 0.23375226769514867 1106 76-77 0.17244772439998357 1106 78-79 0.1431374556221634 1106 80-81 0.11094530383359569 1106 82-83 0.35020904274287545 1106 84-85 0.14251000375483613 1106 86-87 0.26571942039123186 1106 88-89 0.4096338812920841 1106 90-91 0.4475654151655597 1106 92-93 0.40902674522257954 1106 94-95 0.4163427739031462 1106 96-97 0.36380994543030454 1106 98-99 0.45063257494154385 1106 100-101 0.8430467095475294 1107 1 -0.1273661310969203 1107 2 -0.09856065590265217 1107 3 -0.14859909622224166 1107 4 -0.12436647580605609 1107 5 -0.08785201610873372 1107 6 -0.08722741031569115 1107 7 -0.08907080444692639 1107 8 -0.014226758369950687 1107 9 -0.0954506456958697 1107 10-11 -0.10254153158864909 1107 12-13 -0.11055961290620786 1107 14-15 -0.12252400167567856 1107 16-17 -0.13213686521221746 1107 18-19 -0.19099192953424904 1107 20-21 -0.13614035190544627 1107 22-23 -0.1753272102415906 1107 24-25 -0.19742696922370584 1107 26-27 -0.11682386391007071 1107 28-29 -0.08205372417365453 1107 30-31 -0.14578999250918656 1107 32-33 -0.16097961591748344 1107 34-35 -0.12967180673035728 1107 36-37 -0.29943814596477125 1107 38-39 -0.25789770419028457 1107 40-41 -0.25973327273990776 1107 42-43 -0.25969586399168776 1107 44-45 -0.33967620443777946 1107 46-47 -0.323544940194779 1107 48-49 -0.5922545068288017 1107 50-51 -0.5608304855400945 1107 52-53 -0.4800549504097873 1107 54-55 -0.4367462713694934 1107 56-57 -0.4369627949575161 1107 58-59 -0.3823409282457533 1107 60-61 -0.39256163701900704 1107 62-63 -0.40020979474896023 1107 64-65 -0.34975855285946267 1107 66-67 -0.35576440244379626 1107 68-69 -0.33122629094520306 1107 70-71 -0.31831652411075595 1107 72-73 -0.3228356532989274 1107 74-75 -0.30442891676263173 1107 76-77 -0.3548429518268641 1107 78-79 -0.2932470818262054 1107 80-81 -0.2281441265678943 1107 82-83 -0.2786679247865713 1107 84-85 -0.41000026506749165 1107 86-87 -0.2986739613780358 1107 88-89 -0.1193468569085141 1107 90-91 -0.043764538043824075 1107 92-93 0.030644095563335583 1107 94-95 0.06825675826393862 1107 96-97 0.08576949363098763 1107 98-99 0.13158033744958786 1107 100-101 0.6258315304529134 1108 1 -0.08875288385645064 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1711.0 9 3238.0 10 5171.0 11 7955.0 12 11005.0 13 15264.0 14 17860.0 15 23632.0 16 30070.0 17 40097.0 18 37025.0 19 25536.0 20 24106.0 21 25016.0 22 27258.0 23 29809.0 24 34437.0 25 41292.0 26 49654.0 27 61562.0 28 75005.0 29 91486.0 30 111601.0 31 135395.0 32 167840.0 33 211601.0 34 282011.0 35 397225.0 36 598634.0 37 916617.0 38 744458.0 39 73590.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14822645197179 19.376469278267503 14.331183021657857 23.144121248102852 2 16.04382463846109 21.17465295691729 38.53187831655738 24.249644088064237 3 19.00247864759948 25.297442343192657 30.027690272790174 25.67238873641769 4 13.324004442299659 16.84009944230429 36.17832091076406 33.657575204631996 5 13.775551790859941 37.803405083821076 33.60334321025619 14.817699915062784 6 32.035501347232035 35.92200411070181 17.10929113086907 14.933203411197077 7 28.5261216667701 33.03309679971027 20.76471743324741 17.676064100272228 8 26.824089194263546 34.287124833622165 19.72192093336458 19.16686503874971 9 27.16337492508945 16.066216755694825 20.172912531342018 36.597495787873704 10-11 25.369574137590877 26.235155670369508 26.87995370597645 21.515316486063167 12-13 26.243225631957884 23.859831366748146 27.585652742953314 22.31129025834066 14-15 23.325641574861496 24.962839891396847 26.031110519338423 25.680408014403234 16-17 22.521979276516735 28.17074481067695 26.650304071937985 22.656971840868326 18-19 22.38916895808047 26.589434954665148 29.130238406993765 21.89115768026062 20-21 23.270863108442686 25.751085334575745 28.933208968702907 22.044842588278666 22-23 23.05006803313385 25.735582208941842 28.847160253459315 22.367189504464992 24-25 22.83168121035067 25.954744766913574 28.942494634691247 22.27107938804451 26-27 22.58798272677476 26.03592401994639 29.05783534027228 22.31825791300657 28-29 22.419244809057997 26.285069019591134 28.9827055049874 22.312980666363476 30-31 22.69544127059403 26.086133467207475 28.801542393117586 22.416882869080908 32-33 22.422776140886477 26.274787633808533 28.791897804877824 22.510538420427167 34-35 22.80454348452615 26.139139321460085 28.73301105719257 22.323306136821195 36-37 22.904220619241244 25.928015935263975 28.669567349152718 22.498196096342063 38-39 22.638948619736862 26.23032616401045 28.65483891661404 22.47588629963865 40-41 22.73081538208403 26.056785614499343 28.725125218452746 22.487273784963875 42-43 22.574854396956603 26.327239431600173 28.248443525725836 22.84946264571738 44-45 22.65763614899428 26.237068688035414 28.219761791073257 22.88553337189705 46-47 22.803984016785826 26.370358225970207 27.88387982555135 22.941777931692613 48-49 22.908762174124572 26.5872877119118 27.500999772373767 23.00295034158986 50-51 22.63048438253667 26.74878608543263 27.57087179316179 23.04985773886891 52-53 22.88274079514941 26.758800078916583 27.336514387919465 23.021944738014543 54-55 22.463473292942616 26.575587636772536 27.531918608474644 23.42902046181021 56-57 22.548398928883376 26.77850606833324 27.30160630443461 23.37148869834877 58-59 22.683672408447173 26.545335690036016 27.366993766069086 23.40399813544773 60-61 22.619739555063198 26.79693853543952 27.51668651944121 23.066635390056074 62-63 22.52995495641526 26.565954626669914 27.642107739660492 23.261982677254334 64-65 22.279751412764295 26.53778501900204 27.61639269706684 23.566070871166826 66-67 22.34131262579809 26.722467064403993 27.193367586538436 23.74285272325948 68-69 22.270448149531532 26.60327891021935 27.44303681392871 23.683236126320413 70-71 22.345655383890755 26.503043432903855 27.281526835250077 23.86977434795531 72-73 22.318642375426194 26.58061254829241 26.998421205219238 24.102323871062158 74-75 22.154302296824536 26.963628903105814 26.88215355203354 23.999915248036118 76-77 22.190633013438994 26.542700366252483 27.175361483386283 24.09130513692224 78-79 22.13609869615555 26.635573553959464 27.043873348767157 24.184454401117833 80-81 22.240821862772396 26.884235376662986 26.80116221381006 24.073780546754563 82-83 22.311093430009237 26.58753627430367 26.842046884971673 24.259323410715428 84-85 22.125727454356667 26.48010273975775 26.723093103968893 24.671076701916693 86-87 22.11744974303042 26.42807066530086 26.888623788233225 24.56585580343549 88-89 22.058718131386833 26.401390759224576 26.709957911784226 24.829933197604365 90-91 22.06132151159537 26.361880421705564 26.724049250223565 24.852748816475508 92-93 22.121848778413906 26.34537953596679 26.583657598360904 24.949114087258398 94-95 22.02180326580072 26.113449831844825 26.718280488176227 25.146466414178224 96-97 21.974100170337554 26.017614051597736 26.77675776962471 25.231528008439994 98-99 21.89058637597763 26.039435217293143 26.588552671950165 25.481425734779062 100-101 21.974329924897866 25.744129407467142 26.87952455190835 25.40201611572664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2888.0 1 1921.5 2 1220.5 3 1536.0 4 1319.5 5 1354.5 6 2347.5 7 4406.0 8 6565.5 9 7082.0 10 5975.0 11 4977.5 12 4944.0 13 5310.0 14 5685.0 15 6018.5 16 6380.0 17 6712.5 18 7150.0 19 7688.0 20 8357.0 21 9393.5 22 10599.0 23 11986.0 24 13806.5 25 16627.0 26 20553.0 27 24934.0 28 29820.0 29 35370.0 30 41688.0 31 49604.5 32 58590.5 33 65208.0 34 70347.0 35 76495.0 36 85307.5 37 92864.0 38 97589.0 39 102332.0 40 106669.5 41 113345.0 42 119496.5 43 127260.5 44 136872.5 45 143697.0 46 148645.5 47 155963.5 48 171784.0 49 176930.0 50 170193.5 51 168295.5 52 165814.5 53 163961.5 54 160757.0 55 153589.0 56 144176.5 57 131364.0 58 121396.0 59 110234.5 60 93410.0 61 76951.0 62 63914.0 63 49516.5 64 36091.5 65 27667.0 66 19742.5 67 14708.0 68 10615.5 69 6740.0 70 4815.5 71 3430.0 72 2489.5 73 1968.5 74 1474.0 75 1149.5 76 818.5 77 415.0 78 223.0 79 138.0 80 89.0 81 52.5 82 36.5 83 28.5 84 17.5 85 8.0 86 4.5 87 4.5 88 4.5 89 2.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38501937253906693 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.084064685662839E-4 16-17 0.0022693148799439807 18-19 1.7367205713856993E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.9682833142371258E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007977336491231645 36-37 0.01770297169099156 38-39 0.011647605965426756 40-41 0.007653148651239648 42-43 0.00348501927991397 44-45 0.002084064685662839 46-47 0.0018177675313836984 48-49 2.3156274285142657E-5 50-51 3.9365666284742515E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.862817599879958E-4 60-61 6.483756799839943E-4 62-63 0.0 64-65 0.0 66-67 0.0012967513599679886 68-69 8.567821485502782E-4 70-71 0.0020261739999499827 72-73 0.0 74-75 0.0 76-77 0.09834469688900085 78-79 0.03607747533625226 80-81 3.4734411427713983E-5 82-83 0.0 84-85 0.0 86-87 0.15040000148200156 88-89 0.32586666987767005 90-91 0.2794267617988165 92-93 0.0 94-95 5.7890685712856644E-5 96-97 0.0 98-99 2.084064685662839E-4 100-101 7.294226399819937E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4318484.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.134454325080014 #Duplication Level Percentage of deduplicated Percentage of total 1 59.61205489143724 16.77152635567304 2 13.910875391575495 7.827497766523206 3 6.981903553595063 5.892961398922025 4 4.30989470499837 4.850261428947233 5 2.881588086268118 4.053595419840255 6 2.144954337584288 3.620827190408845 7 1.6038974952254623 3.1587346575071713 8 1.1932992425838025 2.685825842930125 9 0.9802269380087005 2.4820335014056947 >10 5.8596999496249 29.524103067422324 >50 0.3391126677239448 6.536054041958238 >100 0.1706078562451731 8.539313216458734 >500 0.008831129985013635 1.6173521304102965 >1k 0.0027236194530836652 1.2881090667792698 >5k 8.253392282071693E-5 0.14131977323055586 >10k+ 2.4760176846215076E-4 1.0104851415829936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18715 0.4333696732464448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14578 0.33757216652880967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10224 0.23674974829129852 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6086 0.1409290852993782 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.3156274285142657E-5 0.0 10-11 0.0 0.0 0.0 3.4734411427713983E-5 0.0 12-13 0.0 0.0 0.0 6.946882285542797E-5 0.0 14-15 0.0 0.0 0.0 8.104695999799929E-5 0.0 16-17 0.0 0.0 0.0 1.6209391999599858E-4 0.0 18-19 0.0 0.0 0.0 2.3156274285142657E-4 0.0 20-21 0.0 0.0 0.0 3.7050038856228245E-4 0.0 22-23 0.0 0.0 0.0 0.0010767667542591335 0.0 24-25 0.0 0.0 0.0 0.0018988144913816979 0.0 26-27 0.0 0.0 0.0 0.003948144765616822 0.0 28-29 0.0 0.0 0.0 0.011184480479723903 0.0 30-31 0.0 0.0 0.0 0.03328714428489257 0.0 32-33 0.0 0.0 0.0 0.07300015468391222 0.0 34-35 0.0 0.0 0.0 0.133426452430992 0.0 36-37 0.0 0.0 0.0 0.23087963276001483 0.0 38-39 0.0 0.0 0.0 0.3782345841735201 0.0 40-41 0.0 0.0 0.0 0.5503783272092706 0.0 42-43 0.0 0.0 0.0 0.7152394219823438 0.0 44-45 0.0 0.0 0.0 0.9136076456460184 0.0 46-47 0.0 0.0 0.0 1.1525456618572627 0.0 48-49 0.0 0.0 0.0 1.4080867267309547 0.0 50-51 0.0 0.0 0.0 1.6834842968041563 0.0 52-53 0.0 0.0 0.0 1.9562883641574218 0.0 54-55 0.0 0.0 0.0 2.247154325453099 0.0 56-57 0.0 0.0 0.0 2.587551557444696 0.0 58-59 0.0 0.0 0.0 2.9611780430354724 0.0 60-61 0.0 0.0 0.0 3.3705115035739395 0.0 62-63 0.0 0.0 0.0 3.7816395753695047 0.0 64-65 0.0 0.0 0.0 4.199181935142054 0.0 66-67 0.0 0.0 0.0 4.658394010490719 0.0 68-69 0.0 0.0 0.0 5.1756935998836635 0.0 70-71 0.0 0.0 0.0 5.739965691664019 0.0 72-73 0.0 0.0 2.3156274285142657E-5 6.316232733524079 0.0 74-75 0.0 0.0 2.3156274285142657E-5 6.895556403589778 0.0 76-77 0.0 2.3156274285142657E-5 2.3156274285142657E-5 7.503119150146208 0.0 78-79 0.0 2.3156274285142657E-5 2.3156274285142657E-5 8.185916631855068 0.0 80-81 0.0 2.3156274285142657E-5 2.3156274285142657E-5 8.91699494544845 0.0 82-83 0.0 2.3156274285142657E-5 2.3156274285142657E-5 9.657185252973033 0.0 84-85 0.0 2.3156274285142657E-5 2.3156274285142657E-5 10.40543394394885 0.0 86-87 0.0 2.3156274285142657E-5 2.3156274285142657E-5 11.16159050259304 0.0 88-89 0.0 2.3156274285142657E-5 2.3156274285142657E-5 11.964302750687509 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 25965 0.0 29.848272 1 GGTATCA 17870 0.0 28.92179 1 GTACATG 21200 0.0 26.715664 1 TACATGG 21795 0.0 25.690495 2 ATCAACG 31495 0.0 24.392288 3 TCAACGC 31515 0.0 24.391867 4 ACATGGG 21955 0.0 24.292946 3 CAACGCA 31705 0.0 24.230728 5 TATCAAC 31930 0.0 24.164007 2 AACGCAG 32080 0.0 23.947481 6 GAGTACT 16210 0.0 22.14667 12-13 CATGGGG 10740 0.0 21.29561 4 CATGGGA 11440 0.0 21.112473 4 ACGCAGA 36130 0.0 21.092348 7 AGTACTT 16860 0.0 20.983242 12-13 CGCAGAG 36195 0.0 20.89715 8 GTACTTT 17630 0.0 20.187914 14-15 AGAGTAC 28920 0.0 19.690783 10-11 GCAGAGT 38510 0.0 19.3824 9 ATGGGGA 5095 0.0 18.440315 5 >>END_MODULE