FastQCFastQC Report
Thu 26 May 2016
SRR938834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324390
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199760.46193798431686317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141720.3277225227141863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99170.22932714209402943No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57480.1329204812701907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA266750.030.5391621
GGTATCA178550.029.6801261
GTACATG215450.026.97931
TACATGG220650.025.931822
TATCAAC321650.025.2528172
ATCAACG320950.025.2191963
TCAACGC321450.025.1652134
CAACGCA322600.025.0166745
ACATGGG220300.024.810053
AACGCAG327200.024.634836
CATGGGA115550.022.7062174
GTACGGT1703.18423E-622.3270153
GAGTACT162150.022.00092512-13
ACGCAGA365450.021.88797
CGCAGAG365450.021.6931678
AGTACTT168750.021.23890112-13
GCAGAGT381700.020.196949
CATGGGG106200.020.05914
GTACTTT177850.019.93875914-15
AGAGTAC290300.019.92368310-11
Produced by FastQC (version 0.11.5)