FastQCFastQC Report
Thu 26 May 2016
SRR938834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324390
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188190.4351827656617465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145260.33590864838740264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102250.23644953392270357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60080.13893288995673378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA265800.030.1766681
GGTATCA180950.028.5522231
GTACATG218400.025.2899671
TCAACGC322250.024.5943894
ATCAACG322850.024.5486833
TATCAAC324300.024.5120932
CAACGCA326250.024.2783035
TACATGG224950.024.2196792
AACGCAG331500.023.9009366
ACATGGG221700.023.1833043
GAGTACT165350.021.66455712-13
ACGCAGA372850.021.033217
CGCAGAG374000.020.8543728
AGTACTT171000.020.85162512-13
GTACTTT179100.019.90858714-15
CATGGGG110500.019.8423084
CATGGGA119200.019.7875794
AGAGTAC298400.019.541610-11
GCAGAGT397950.019.229719
ATGGGGA48400.018.434235
Produced by FastQC (version 0.11.5)