##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938834_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4324390 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.072831543870926 33.0 31.0 34.0 30.0 34.0 2 32.26444770245052 34.0 31.0 34.0 30.0 34.0 3 32.34376617280125 34.0 31.0 34.0 30.0 34.0 4 35.973205931934906 37.0 35.0 37.0 35.0 37.0 5 35.875609970423575 37.0 35.0 37.0 35.0 37.0 6 35.89938696556046 37.0 35.0 37.0 35.0 37.0 7 35.811427507694724 37.0 35.0 37.0 33.0 37.0 8 35.7989392723598 37.0 35.0 37.0 33.0 37.0 9 37.441372077911566 39.0 37.0 39.0 34.0 39.0 10-11 37.37183729959601 39.0 37.0 39.0 34.0 39.0 12-13 37.291960947093116 39.0 37.0 39.0 33.5 39.0 14-15 38.50708365804195 40.0 38.0 41.0 34.0 41.0 16-17 38.44219103734862 40.0 38.0 41.0 33.0 41.0 18-19 38.56942620808947 40.0 38.0 41.0 34.0 41.0 20-21 38.56845890403039 40.0 38.0 41.0 34.0 41.0 22-23 38.48738041665992 40.0 38.0 41.0 34.0 41.0 24-25 38.402383110681505 40.0 38.0 41.0 34.0 41.0 26-27 38.223618938162375 40.0 38.0 41.0 33.0 41.0 28-29 38.05196837010538 40.0 37.0 41.0 33.0 41.0 30-31 37.81962230511124 40.0 37.0 41.0 33.0 41.0 32-33 37.66331817898016 40.0 37.0 41.0 32.5 41.0 34-35 37.63643010921771 40.0 37.0 41.0 32.5 41.0 36-37 37.5182995752002 40.0 37.0 41.0 32.0 41.0 38-39 37.38705875279519 40.0 36.0 41.0 32.0 41.0 40-41 37.20139187261094 40.0 36.0 41.0 31.0 41.0 42-43 36.98794974551324 40.0 36.0 41.0 31.0 41.0 44-45 36.71827783803033 39.5 35.5 41.0 30.5 41.0 46-47 36.37300613496933 39.0 35.0 41.0 30.0 41.0 48-49 36.04678879564516 39.0 35.0 41.0 29.5 41.0 50-51 35.78868337499624 39.0 35.0 41.0 28.0 41.0 52-53 35.55363565728345 39.0 35.0 40.0 28.0 41.0 54-55 35.2623372313783 38.0 34.5 40.0 27.0 41.0 56-57 34.97420480113958 38.0 34.0 40.0 26.5 41.0 58-59 34.65281866344155 38.0 34.0 40.0 26.0 41.0 60-61 34.27287282136902 37.0 34.0 40.0 26.0 41.0 62-63 33.89915502533305 36.5 33.0 39.5 25.5 41.0 64-65 33.49047033223183 36.0 33.0 39.0 24.5 40.5 66-67 33.078851121198596 35.5 32.5 39.0 23.0 40.0 68-69 32.74309555798621 35.0 32.0 38.5 22.5 40.0 70-71 32.32078801865697 35.0 32.0 37.5 22.0 40.0 72-73 31.849909004506994 35.0 31.0 37.0 20.5 39.0 74-75 31.375727674885937 35.0 31.0 36.5 19.0 39.0 76-77 29.44726897897738 32.5 28.5 35.0 16.0 36.5 78-79 30.406239839607437 34.0 30.0 35.0 16.0 37.0 80-81 30.47211294540964 34.0 31.0 35.0 16.5 37.0 82-83 30.175538284012312 34.0 30.0 35.0 11.5 37.0 84-85 29.80217938252563 34.0 30.0 35.0 7.0 36.0 86-87 29.250971119626122 34.0 29.0 35.0 2.0 36.0 88-89 28.678606809284084 34.0 29.0 35.0 2.0 35.0 90-91 28.18051944898587 33.5 28.0 35.0 2.0 35.0 92-93 27.89685204155962 33.0 27.0 35.0 2.0 35.0 94-95 27.567559586438783 33.0 27.0 35.0 2.0 35.0 96-97 26.974728574434778 33.0 26.5 35.0 2.0 35.0 98-99 26.178508992019683 33.0 25.0 35.0 2.0 35.0 100-101 22.659429306792404 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07017265788370253 1101 2 0.1264280135753495 1101 3 0.03391387004903379 1101 4 0.0072880930932583965 1101 5 -0.10898929884258735 1101 6 0.06494588208772001 1101 7 0.11860321356591186 1101 8 0.14882967763664823 1101 9 0.15350548031432254 1101 10-11 0.17763083789621703 1101 12-13 0.11847568182262336 1101 14-15 0.17320613178296895 1101 16-17 0.27275367648409343 1101 18-19 0.28053549336856065 1101 20-21 0.21158261325552274 1101 22-23 0.13633291305169593 1101 24-25 0.17606803693833228 1101 26-27 0.020571828002260872 1101 28-29 0.10984622890156714 1101 30-31 0.1543851295341625 1101 32-33 0.23281911530936128 1101 34-35 0.2545158826799039 1101 36-37 0.24749361138233184 1101 38-39 0.28020670214029053 1101 40-41 0.28956329239377965 1101 42-43 0.2941676481979627 1101 44-45 0.23953381193484802 1101 46-47 0.17955951723002528 1101 48-49 0.1649520796265378 1101 50-51 0.054595202833510825 1101 52-53 -0.09085019463152122 1101 54-55 -0.0020586662986019633 1101 56-57 -0.014888686346502311 1101 58-59 0.06406438611422516 1101 60-61 0.11898466214442038 1101 62-63 -0.06437546050172926 1101 64-65 -0.09817633303619999 1101 66-67 -0.09667932780594413 1101 68-69 0.002753231327410788 1101 70-71 0.022621287283278946 1101 72-73 -0.09527811099674821 1101 74-75 -0.028264589213979008 1101 76-77 -0.07219320220967873 1101 78-79 -0.15203641105636478 1101 80-81 -0.028874553371633027 1101 82-83 0.08426763867325704 1101 84-85 0.11783919519831443 1101 86-87 0.09193602016078728 1101 88-89 0.21751969848009267 1101 90-91 0.07179863104616757 1101 92-93 0.10258059287003363 1101 94-95 -0.04249207065975469 1101 96-97 -0.23575271487995408 1101 98-99 -0.32538991404522477 1101 100-101 0.2809472743409316 1102 1 0.20678755053268816 1102 2 0.15472633389479284 1102 3 0.17935900054740017 1102 4 0.14935098996623708 1102 5 -0.13105971868998978 1102 6 -0.06450050829612053 1102 7 0.10753672499587452 1102 8 0.07496348688071919 1102 9 0.1779828609944829 1102 10-11 0.21464442979049636 1102 12-13 0.22492829981943885 1102 14-15 0.3257667518407388 1102 16-17 0.36205478675528724 1102 18-19 0.24524628059746334 1102 20-21 0.26065205340282915 1102 22-23 0.22647756931633722 1102 24-25 0.216380031086004 1102 26-27 0.2385030077356305 1102 28-29 0.23624504992658757 1102 30-31 0.26510863394769757 1102 32-33 0.3236099286099474 1102 34-35 0.3282362884950345 1102 36-37 0.23304310676403617 1102 38-39 0.2820675516073763 1102 40-41 0.39658510700719773 1102 42-43 0.39248390831601654 1102 44-45 0.31468806194678933 1102 46-47 0.31633845027975127 1102 48-49 0.2923633455854784 1102 50-51 0.3693789285308213 1102 52-53 0.21205509138496126 1102 54-55 0.31190112005674564 1102 56-57 0.2744734345343076 1102 58-59 0.4300148311938301 1102 60-61 0.3225654404272831 1102 62-63 0.21849016028136958 1102 64-65 0.27201527224659827 1102 66-67 0.35209395415834166 1102 68-69 0.2991950208178693 1102 70-71 0.36122298344837134 1102 72-73 0.3020701727629067 1102 74-75 0.1728123449290031 1102 76-77 0.13713752847962368 1102 78-79 0.13168431272001158 1102 80-81 0.40984091886951646 1102 82-83 0.37906735672845926 1102 84-85 0.5929171287630446 1102 86-87 0.5554479842717583 1102 88-89 0.4604268773146565 1102 90-91 0.3137646906100251 1102 92-93 0.4011640784232391 1102 94-95 0.3871710220957496 1102 96-97 0.4911611573549379 1102 98-99 0.6765746609328787 1102 100-101 1.232625273096339 1103 1 0.1778106924885492 1103 2 0.18556435376982705 1103 3 0.16406333601755563 1103 4 0.020911920123488414 1103 5 0.00907072776590212 1103 6 0.08491628781789728 1103 7 0.11714565750516925 1103 8 0.08688832185168138 1103 9 0.12857175261326148 1103 10-11 0.17759285372974887 1103 12-13 0.20904176158211385 1103 14-15 0.19824489145951674 1103 16-17 0.1704605608437717 1103 18-19 0.013670768049621529 1103 20-21 0.14527083380723838 1103 22-23 0.21560955179366914 1103 24-25 0.16897800450733058 1103 26-27 0.16169768214962232 1103 28-29 0.12165035537478985 1103 30-31 0.1756787655000167 1103 32-33 0.3575857644745426 1103 34-35 0.26806958288158 1103 36-37 0.2389619071783855 1103 38-39 0.17692202709972094 1103 40-41 0.3042272237400425 1103 42-43 0.4112711068807684 1103 44-45 0.3541427468122009 1103 46-47 0.31926329598123004 1103 48-49 0.3514557758024708 1103 50-51 0.39361945635687334 1103 52-53 0.3660378915253162 1103 54-55 0.3412619417634488 1103 56-57 0.15478721835720677 1103 58-59 0.27360326351289643 1103 60-61 0.26843201266535743 1103 62-63 0.2498886414510011 1103 64-65 0.34507419999388134 1103 66-67 0.2514542482347011 1103 68-69 0.26682617796236485 1103 70-71 0.2062078570258663 1103 72-73 0.3499016148277825 1103 74-75 0.3236359514330793 1103 76-77 0.22147315097251052 1103 78-79 0.08763585234702376 1103 80-81 0.27045535413519417 1103 82-83 0.2765282093646384 1103 84-85 0.3558732475431441 1103 86-87 0.3209165464268082 1103 88-89 0.20945589014496946 1103 90-91 0.2459596600855818 1103 92-93 0.17547606429986828 1103 94-95 0.27061975858231335 1103 96-97 0.3665961396470756 1103 98-99 0.5909540166978999 1103 100-101 1.3041507369315042 1104 1 0.041514629596186126 1104 2 0.07278023657438126 1104 3 0.04058848487508726 1104 4 0.05011108620456639 1104 5 -0.0727573585635497 1104 6 -0.015192828523126423 1104 7 0.004388048328394234 1104 8 -0.013322658636582219 1104 9 -0.05671046192330209 1104 10-11 0.013179368650511947 1104 12-13 -0.07638583026441381 1104 14-15 -0.04413134401838903 1104 16-17 0.054006250081428675 1104 18-19 0.038373323906100154 1104 20-21 -0.023258312926309088 1104 22-23 -0.02793120261787152 1104 24-25 -0.011837025372535948 1104 26-27 0.05594444179440927 1104 28-29 -0.07473646830603542 1104 30-31 -0.16167661783678255 1104 32-33 -0.13881923137004293 1104 34-35 -0.08612806190597411 1104 36-37 -0.024211286045897396 1104 38-39 -0.028861555767420555 1104 40-41 0.03822292410607986 1104 42-43 0.011254351812361563 1104 44-45 0.06778012211823636 1104 46-47 -0.018631917320796276 1104 48-49 0.03506148319562641 1104 50-51 -0.05308933972060714 1104 52-53 -0.12354362354966497 1104 54-55 -0.07166313473886277 1104 56-57 -0.16429572089858624 1104 58-59 0.04256237364371884 1104 60-61 0.016849492286269196 1104 62-63 0.006287785525408651 1104 64-65 -0.03613511582415896 1104 66-67 -0.13375861198885275 1104 68-69 -0.051957920894210474 1104 70-71 -0.14170880443089828 1104 72-73 -0.1425420160372184 1104 74-75 -0.12487213893092175 1104 76-77 -0.030412377733252782 1104 78-79 -0.07953813445064739 1104 80-81 -0.06008188337915499 1104 82-83 -0.039935770989419694 1104 84-85 -0.06311007824623971 1104 86-87 -0.010432019847826268 1104 88-89 -0.057433427206863286 1104 90-91 0.042495612211101985 1104 92-93 0.06649876533359844 1104 94-95 -0.1072858912100827 1104 96-97 -0.11814024310870508 1104 98-99 0.2799251804749403 1104 100-101 1.1679967170071457 1105 1 0.02474656345452786 1105 2 0.0912146611283049 1105 3 0.09623976153503122 1105 4 0.02918418143570989 1105 5 -0.040177301650054176 1105 6 0.01575137094018686 1105 7 0.013543041887494667 1105 8 0.062045705376171156 1105 9 0.08066116634135057 1105 10-11 0.06648466705699718 1105 12-13 0.04874963943417754 1105 14-15 0.02915654612623797 1105 16-17 0.04877857986821965 1105 18-19 0.08508165358905018 1105 20-21 0.026946351774022048 1105 22-23 0.0669766779870784 1105 24-25 -0.016838371957568654 1105 26-27 0.015981691905551543 1105 28-29 0.0799468895254094 1105 30-31 0.04380657905341678 1105 32-33 0.11569766908326073 1105 34-35 0.05133506924492082 1105 36-37 0.026021364909531997 1105 38-39 0.1013365057990967 1105 40-41 0.11594189018835266 1105 42-43 0.09179522472393842 1105 44-45 0.09441314038311788 1105 46-47 0.05098482511156277 1105 48-49 0.037847291811822004 1105 50-51 0.07097543264162454 1105 52-53 0.10901680242547229 1105 54-55 0.12663716670149938 1105 56-57 0.13168391159727122 1105 58-59 0.18797717050406249 1105 60-61 0.15867028615492984 1105 62-63 0.12921144875586066 1105 64-65 0.011384085180431214 1105 66-67 0.059659588649076056 1105 68-69 0.04778330764560934 1105 70-71 0.008567488858567174 1105 72-73 0.12727703862764272 1105 74-75 0.18379930081183105 1105 76-77 0.11335690844688884 1105 78-79 -0.08040700627197594 1105 80-81 -0.011178295622368495 1105 82-83 0.16292693898861543 1105 84-85 0.07588191362882313 1105 86-87 0.20551114128486603 1105 88-89 0.18186625123512457 1105 90-91 0.12388775399514529 1105 92-93 0.3157725828534801 1105 94-95 0.3533596746314345 1105 96-97 0.3904675988833297 1105 98-99 0.5426437571832388 1105 100-101 1.1582624485272923 1106 1 0.11356718854022319 1106 2 0.09074873111332238 1106 3 0.053667292694008495 1106 4 0.027789658345803048 1106 5 -0.006053230372764062 1106 6 0.10234414012120396 1106 7 0.06627081451074446 1106 8 0.09839441745953081 1106 9 0.07698210826983143 1106 10-11 0.07986580372087815 1106 12-13 0.04236214600179267 1106 14-15 0.00512018377590806 1106 16-17 0.10256178639183844 1106 18-19 -0.023144165809945605 1106 20-21 -0.015235560371650081 1106 22-23 8.616237131136018E-4 1106 24-25 -0.02009822265668504 1106 26-27 0.04448395585352927 1106 28-29 0.10585830256539452 1106 30-31 0.03736699583806313 1106 32-33 0.045298625564718975 1106 34-35 0.05573721336306647 1106 36-37 0.01604952917327296 1106 38-39 0.09712208195409744 1106 40-41 0.21626730483754386 1106 42-43 0.15521465217503305 1106 44-45 0.11615026645418425 1106 46-47 0.06632097507869616 1106 48-49 0.025665957346781454 1106 50-51 0.02778021994418367 1106 52-53 0.06043500316088313 1106 54-55 0.07162913725202458 1106 56-57 0.08746056740934449 1106 58-59 0.09227307117037498 1106 60-61 0.07853869839196648 1106 62-63 0.12180720631916842 1106 64-65 0.02126926882375102 1106 66-67 0.019620228582297727 1106 68-69 0.07031093312105696 1106 70-71 0.1446021480363342 1106 72-73 0.15166488385404264 1106 74-75 0.27181882185340456 1106 76-77 0.29312569826095825 1106 78-79 0.21643368745918323 1106 80-81 0.11807421676976304 1106 82-83 0.25777042902585023 1106 84-85 0.3199635653100188 1106 86-87 0.4145451586874671 1106 88-89 0.421876317605129 1106 90-91 0.42367526709148606 1106 92-93 0.542977637486306 1106 94-95 0.6037504387525097 1106 96-97 0.7762144808081075 1106 98-99 1.0555021291859106 1106 100-101 1.512829407933875 1107 1 0.04799999308944791 1107 2 0.027815060325430352 1107 3 0.027289000616050885 1107 4 0.0065590715756371765 1107 5 -0.04280114056466289 1107 6 -0.06496734975472407 1107 7 -0.009092382742018401 1107 8 0.030925112970457747 1107 9 0.03429275304730339 1107 10-11 -0.032902231340557364 1107 12-13 0.10327581728236623 1107 14-15 0.05522717772807795 1107 16-17 0.014359697392102078 1107 18-19 0.06555781651969994 1107 20-21 -0.0033023316156359783 1107 22-23 0.03246830581527149 1107 24-25 0.05197031467476165 1107 26-27 0.042999856888371824 1107 28-29 0.08534446842613619 1107 30-31 0.061357081512277034 1107 32-33 0.08211163944846334 1107 34-35 0.10169980778573517 1107 36-37 0.10870906624190724 1107 38-39 0.058620350216294526 1107 40-41 -0.10556309272644882 1107 42-43 -0.036554354112105614 1107 44-45 0.028381666548725093 1107 46-47 -0.01890123659750742 1107 48-49 0.13591364055552901 1107 50-51 -0.060884174119969714 1107 52-53 -0.12748852466052796 1107 54-55 -0.05768892949932791 1107 56-57 0.010648411374489797 1107 58-59 0.06561689623693212 1107 60-61 0.14824379065331073 1107 62-63 0.02559909728076093 1107 64-65 -0.05145525259271011 1107 66-67 0.04258721981199187 1107 68-69 -0.0782974365769249 1107 70-71 -0.11550446697307137 1107 72-73 0.011209266134759588 1107 74-75 0.11616999662440719 1107 76-77 0.04802266637718233 1107 78-79 0.1119385899227332 1107 80-81 0.273698790682289 1107 82-83 0.25575514717735714 1107 84-85 0.22472997158061503 1107 86-87 0.25076579272755595 1107 88-89 0.2661436710541203 1107 90-91 0.3880561524027506 1107 92-93 0.5779882761894761 1107 94-95 0.7473714015394641 1107 96-97 0.7820725635321821 1107 98-99 0.903654104918477 1107 100-101 1.6389507380606965 1108 1 0.11892006671894251 1108 2 0.05795650514287587 1108 3 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-1.0793697604201355 2316 86-87 -1.2250702985557496 2316 88-89 -1.6377022774324779 2316 90-91 -2.1531679209488104 2316 92-93 -1.760427133143228 2316 94-95 -1.6284596387652286 2316 96-97 -1.823152725658872 2316 98-99 -1.8543343601963613 2316 100-101 -1.840357506762988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 25.0 4 59.0 5 138.0 6 315.0 7 738.0 8 1772.0 9 3230.0 10 5313.0 11 8085.0 12 11430.0 13 15730.0 14 18401.0 15 24101.0 16 30652.0 17 41327.0 18 38417.0 19 26681.0 20 25395.0 21 26679.0 22 28756.0 23 31972.0 24 37057.0 25 43896.0 26 52942.0 27 63793.0 28 78152.0 29 94879.0 30 114553.0 31 139348.0 32 171833.0 33 216838.0 34 290350.0 35 408637.0 36 613018.0 37 911143.0 38 689373.0 39 59358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13642001334631 19.377553860984666 14.309919497967341 23.176106627701685 2 16.01966982626451 21.137755845333096 38.56791362481182 24.274660703590563 3 18.995395882425036 25.230494936858143 29.99343259974239 25.780676580974426 4 13.328931941846134 16.832269984899604 36.14828912285895 33.69050895039531 5 13.76911493596164 37.8263504495523 33.563849842361776 14.840684772124288 6 32.07014189425758 35.89327150464155 17.122634768093842 14.91395183300703 7 28.528694220456526 33.04096069040951 20.74521493204822 17.68513015708574 8 26.84082610495353 34.250888564629925 19.744657628012273 19.16362770240427 9 27.14972516354908 16.054888666378382 20.166705593158802 36.62868057691374 10-11 25.354547115315683 26.192330016487876 26.898741787859098 21.554381080337343 12-13 26.25403814179572 23.818388258228328 27.59759179907455 22.3299818009014 14-15 23.33884085385453 24.92426677519835 26.040828879911388 25.69606349103573 16-17 22.530022408406506 28.182490917542154 26.6214991200827 22.665987553968648 18-19 22.390753377933073 26.60940618214361 29.125657029083875 21.87418341083945 20-21 23.29526245320149 25.711094512752087 28.9262184955566 22.06742453848982 22-23 23.03745730611716 25.72281870969085 28.85815109183029 22.3815728923617 24-25 22.8400074230423 25.934826382324832 28.933076417445534 22.292089777187325 26-27 22.58334854765211 26.03008261057129 29.034632106209397 22.3519367355672 28-29 22.442309782420182 26.273659406297767 28.91429773910309 22.369733072178967 30-31 22.68842541953894 26.077203952464973 28.794118939318608 22.440251688677478 32-33 22.46479850337273 26.253483150224653 28.76130506267936 22.520413283723254 34-35 22.83407962028402 26.144707514256233 28.708713125631057 22.312499739828688 36-37 22.915069575451376 25.941402513706414 28.628389827911388 22.515138082930825 38-39 22.645114318476487 26.20912216528154 28.646470070988517 22.499293445253453 40-41 22.728182027583692 26.05927284063567 28.69653913363632 22.516005998144312 42-43 22.607399160670063 26.289784945552714 28.20865973974135 22.89415615403588 44-45 22.669434177026236 26.179162571037317 28.222963036022534 22.92844021591391 46-47 22.814513627265608 26.415737252430326 27.846086481860276 22.923662638443783 48-49 22.93139060926771 26.559718484307076 27.510032823764092 22.998858082661126 50-51 22.66449032787992 26.729042352351364 27.5406369641207 23.06583035564801 52-53 22.913856058311115 26.7237460081075 27.320893813925203 23.041504119656185 54-55 22.49475648588587 26.561665344707574 27.513475889084933 23.43010228032162 56-57 22.563483112679187 26.76951321346453 27.23117914668577 23.43582452717051 58-59 22.709907622435395 26.515511788126485 27.392182333968684 23.382398255469436 60-61 22.675288693002422 26.74671248245514 27.50421544798219 23.073783376560247 62-63 22.53753368860072 26.55558403768799 27.62059995858336 23.28628231512793 64-65 22.289893842121547 26.548511509571227 27.57418864587539 23.587406002431834 66-67 22.398537694456724 26.69883575437883 27.153065801321212 23.749560749843234 68-69 22.314877776106375 26.59465941876748 27.39993101900515 23.690531786120992 70-71 22.39865321889702 26.48164999184855 27.235953783023483 23.883743006230944 72-73 22.34190255735491 26.553467656709962 26.982649576009564 24.12198020992556 74-75 22.211234104035547 26.937890114809843 26.79731465962194 24.053561121532674 76-77 22.22541399238618 26.537670304976146 27.149348697638224 24.087567004999446 78-79 22.156435694006383 26.644562856335654 27.030644287282378 24.168357162375585 80-81 22.271189203907877 26.870674559195994 26.740955416921764 24.117180819974365 82-83 22.341478451438586 26.572623994964328 26.802936118245775 24.28296143535131 84-85 22.173508864834115 26.48609399244749 26.669079338357548 24.671317804360847 86-87 22.11864317518572 26.412371489608805 26.877736432324795 24.59124890288068 88-89 22.109901458255372 26.366603138135392 26.681391361860257 24.84210404174898 90-91 22.12912950838473 26.32677527478982 26.71421812412565 24.829877092699807 92-93 22.163320899922994 26.300559387841467 26.589306650448503 24.946813061787037 94-95 22.043449998415955 26.11392449263743 26.68627336263546 25.15635214631115 96-97 22.017609906983285 25.988730418020232 26.729471758289737 25.26418791670675 98-99 21.898834286454274 25.98349131322568 26.560948480594952 25.556725919725093 100-101 22.090839432427273 25.538529596797847 26.911697252612505 25.45893371816237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3029.0 1 1985.0 2 1191.5 3 1445.5 4 1248.5 5 1318.5 6 2297.0 7 4280.0 8 6369.5 9 6905.5 10 5778.0 11 4860.0 12 4951.0 13 5258.5 14 5659.5 15 6033.0 16 6326.0 17 6614.5 18 6943.5 19 7532.5 20 8276.5 21 9248.0 22 10589.0 23 12025.0 24 13806.5 25 16717.5 26 20602.5 27 24912.0 28 29607.0 29 35139.5 30 41550.5 31 49273.5 32 58382.0 33 65304.0 34 70224.5 35 76361.5 36 85543.0 37 93240.5 38 98298.0 39 102885.0 40 106982.0 41 113572.0 42 119154.0 43 127117.5 44 136797.0 45 143056.0 46 148424.5 47 156632.5 48 172377.5 49 176989.0 50 169761.0 51 167935.5 52 165783.0 53 163910.0 54 160951.5 55 153984.0 56 144687.0 57 131914.0 58 122063.0 59 110803.0 60 93999.0 61 77766.0 62 64206.0 63 49800.0 64 36737.0 65 28103.0 66 19928.5 67 14726.0 68 10696.5 69 7023.0 70 5089.0 71 3532.0 72 2520.5 73 2009.0 74 1541.5 75 1205.5 76 858.5 77 464.5 78 265.0 79 167.5 80 104.5 81 69.0 82 58.0 83 40.0 84 25.0 85 18.5 86 16.0 87 12.0 88 7.5 89 5.0 90 3.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23330458168666565 2 0.0 3 0.0 4 0.0 5 0.02774957855327572 6 0.004254935378168944 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027749578553275724 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.046813539019376E-4 26-27 0.0014915398472385702 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007307389019029273 36-37 0.01791004049126004 38-39 0.013689792086282689 40-41 0.008926114434637023 42-43 0.007989566158463968 44-45 0.0038502540242670064 46-47 6.128031930515055E-4 48-49 0.0019424704987293007 50-51 4.624929758879287E-5 52-53 0.0 54-55 0.0 56-57 0.0011793570885142183 58-59 4.046813539019376E-4 60-61 0.0026940215845471845 62-63 9.59672924967452E-4 64-65 0.0017574733083741292 66-67 0.006000846362145875 68-69 2.4280881234116258E-4 70-71 1.1562324397198218E-4 72-73 0.0 74-75 5.087422734767216E-4 76-77 0.05806599312272945 78-79 0.14175409710965015 80-81 0.0010984208177338306 82-83 9.481106005702538E-4 84-85 0.0 86-87 0.10532121293407856 88-89 0.3538649381762514 90-91 0.39117655900601006 92-93 4.6249297588792874E-4 94-95 3.931190295047394E-4 96-97 1.387478927663786E-4 98-99 0.0 100-101 0.001052171520145038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4324390.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.005214250782295 #Duplication Level Percentage of deduplicated Percentage of total 1 59.319789002361304 16.612634003223274 2 13.99746448664513 7.840039838324266 3 7.021252413921625 5.898950344820924 4 4.294947974612457 4.8112375289994125 5 2.987013643402527 4.182597852674879 6 2.1578195063283148 3.6258118553545042 7 1.594000470700831 3.1248227288477217 8 1.2501084554889643 2.8007644106146388 9 0.978748450685901 2.4669054053171897 >10 5.877466849369343 29.544150121141634 >50 0.3395904228565672 6.525546221785892 >100 0.1700334811109533 8.53891622972436 >500 0.00886505745048385 1.6411431788504454 >1k 0.0025683810579665675 1.2361417486376454 >5k 8.285100186988922E-5 0.13940122430015336 >10k+ 2.4855300560966767E-4 1.010937307383103 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18819 0.4351827656617465 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14526 0.33590864838740264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10225 0.23644953392270357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6008 0.13893288995673378 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3124648794396436E-5 0.0 12-13 0.0 0.0 0.0 4.624929758879287E-5 0.0 14-15 0.0 0.0 0.0 5.781162198599109E-5 0.0 16-17 0.0 0.0 0.0 1.6187254156077504E-4 0.0 18-19 0.0 0.0 0.0 2.8905810992995546E-4 0.0 20-21 0.0 0.0 0.0 3.5843205631314474E-4 0.0 22-23 0.0 0.0 0.0 8.440496809954699E-4 0.0 24-25 0.0 0.0 0.0 0.0017459109839769308 0.0 26-27 0.0 0.0 0.0 0.0035265089411454565 0.0 28-29 0.0 0.0 0.0 0.010648900769819558 0.0 30-31 0.0 0.0 0.0 0.03109109030406601 0.0 32-33 0.0 0.0 0.0 0.0687380185413434 0.0 34-35 0.0 0.0 0.0 0.12639933031017092 0.0 36-37 0.0 0.0 0.0 0.22044727695698124 0.0 38-39 0.0 0.0 0.0 0.36561225976380485 0.0 40-41 0.0 0.0 0.0 0.52901102814501 0.0 42-43 0.0 0.0 0.0 0.6890914094242193 0.0 44-45 0.0 0.0 0.0 0.8790488369457888 0.0 46-47 0.0 0.0 0.0 1.1082719181202436 0.0 48-49 0.0 0.0 0.0 1.3539019376143226 0.0 50-51 0.0 0.0 0.0 1.6209916311896013 0.0 52-53 0.0 0.0 0.0 1.8924171964138294 0.0 54-55 0.0 0.0 0.0 2.1770700607484526 0.0 56-57 0.0 0.0 0.0 2.5078797240766906 0.0 58-59 0.0 0.0 0.0 2.8748332134705703 0.0 60-61 0.0 0.0 0.0 3.2778727173081057 0.0 62-63 0.0 0.0 0.0 3.683756552947352 0.0 64-65 0.0 0.0 0.0 4.092970338012991 0.0 66-67 0.0 0.0 0.0 4.5500174591098395 0.0 68-69 0.0 0.0 0.0 5.060147211514225 0.0 70-71 0.0 0.0 0.0 5.620827446183161 0.0 72-73 0.0 0.0 0.0 6.181634866420467 0.0 74-75 0.0 0.0 0.0 6.7507787225481515 0.0 76-77 0.0 0.0 0.0 7.353719252888847 0.0 78-79 0.0 0.0 0.0 8.018865088486468 0.0 80-81 0.0 0.0 0.0 8.741163493579442 0.0 82-83 0.0 0.0 0.0 9.47288519305613 0.0 84-85 0.0 0.0 0.0 10.213822065077387 0.0 86-87 0.0 0.0 0.0 10.969639648597838 0.0 88-89 0.0 0.0 0.0 11.768480641200261 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 26580 0.0 30.176668 1 GGTATCA 18095 0.0 28.552223 1 GTACATG 21840 0.0 25.289967 1 TCAACGC 32225 0.0 24.594389 4 ATCAACG 32285 0.0 24.548683 3 TATCAAC 32430 0.0 24.512093 2 CAACGCA 32625 0.0 24.278303 5 TACATGG 22495 0.0 24.219679 2 AACGCAG 33150 0.0 23.900936 6 ACATGGG 22170 0.0 23.183304 3 GAGTACT 16535 0.0 21.664557 12-13 ACGCAGA 37285 0.0 21.03321 7 CGCAGAG 37400 0.0 20.854372 8 AGTACTT 17100 0.0 20.851625 12-13 GTACTTT 17910 0.0 19.908587 14-15 CATGGGG 11050 0.0 19.842308 4 CATGGGA 11920 0.0 19.787579 4 AGAGTAC 29840 0.0 19.5416 10-11 GCAGAGT 39795 0.0 19.22971 9 ATGGGGA 4840 0.0 18.43423 5 >>END_MODULE